Viki De Neef, Wim Van Caeneghem, Malvine Marichal, Marc Van Montagu, Dominique Van Der Straeten
{"title":"基于聚合酶链反应的转基因拟南芥筛选方法研究","authors":"Viki De Neef, Wim Van Caeneghem, Malvine Marichal, Marc Van Montagu, Dominique Van Der Straeten","doi":"10.1016/S1050-3862(98)00012-6","DOIUrl":null,"url":null,"abstract":"<div><p>A simple and rapid PCR-based method for screening transformed <em>Arabidopsis</em> plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 1","pages":"Pages 1-4"},"PeriodicalIF":0.0000,"publicationDate":"1999-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00012-6","citationCount":"4","resultStr":"{\"title\":\"A polymerase chain reaction-based screening method for transgenic Arabidopsis\",\"authors\":\"Viki De Neef, Wim Van Caeneghem, Malvine Marichal, Marc Van Montagu, Dominique Van Der Straeten\",\"doi\":\"10.1016/S1050-3862(98)00012-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A simple and rapid PCR-based method for screening transformed <em>Arabidopsis</em> plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"15 1\",\"pages\":\"Pages 1-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00012-6\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386298000126\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386298000126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A polymerase chain reaction-based screening method for transgenic Arabidopsis
A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.