Detection of foetal cells in maternal blood and prenatal sex determination by in situ hybridization. Procedure verification

Genetic analysis, techniques and applications Pub Date : 1999-04-01 DOI:10.1016/S1050-3862(98)00036-9

J. Chiesa , C. Ferrer , M. Hoffet , P. Mares , J.P. Bureau

引用次数: 0

Abstract

We describe an enrichment of foetal cells from maternal blood with a combination of double density gradient and Magnetic Activated Cell Sorting (MACS) of CD71, glycophorin A (GPA), CD34 and CD36 antibodies labeled cells followed by fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes for determination of foetal sex.

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原位杂交检测母体血液中胎儿细胞及产前性别测定。过程验证

研究人员利用双密度梯度和CD71、糖蛋白a (GPA)、CD34和CD36抗体标记细胞的磁活化细胞分选(MACS)技术，对母体血液中的胎儿细胞进行富集，然后用染色体特异性DNA探针进行荧光原位杂交(FISH)测定胎儿性别。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Genetic analysis, techniques and applications

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