Sensors & diagnostics最新文献_第9页

Synthesis and characterization of La QDs: sensors for anions and H2O2† La QDs 的合成与表征：阴离子和 H2O2 传感器

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-26 DOI: 10.1039/D4SD00142G

Amit Sahoo and Achyuta N. Acharya

{"title":"Synthesis and characterization of La QDs: sensors for anions and H2O2†","authors":"Amit Sahoo and Achyuta N. Acharya","doi":"10.1039/D4SD00142G","DOIUrl":"10.1039/D4SD00142G","url":null,"abstract":"The development of sensitive and accurate fluorescence sensors for the detection of anions and reactive oxygen species (ROS, H2O2) is essential as they play significant roles in biological and chemical processes. In this work, semiconductor La QDs were synthesized. The synthesized La QDs were determined to be pure with 100% La element using EDS technique. La QDs were observed in both cubic and hexagonal lattice configurations through powder XRD analysis. The morphology of the La QDs was characterized using HRTEM and FESEM data as tiny, spherical, homogenous QDs with a diameter ranging from 2 to 6 nm. The fluorescence characteristics of the synthesized La QDs were examined by studying their sensing properties that increased with an increase in anion concentration and decreased with an increase in [H2O2]. The variation in emission intensity at 315 nm and 440.5 nm satisfied the Stern–Volmer equation. The LOD and LOQ of H2O2 and anion sensing with La QDs were studied in the μM range. The Langmuir binding plots and FTIR spectra supported the concept that the surface functionalization of La QDs occurred in the presence of anions. With two band gap energies of about 3.26 eV and 4.66 eV, the synthesized La QDs are a mixture of two (binary) semiconductors.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1476-1493"},"PeriodicalIF":3.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00142g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized gadolinium-DO3A loading in RAFT-polymerized copolymers for superior MR imaging of aging blood–brain barrier† 优化 RAFT 聚合共聚物中的钆-DOTA 负载，为老化血脑屏障提供优质磁共振成像

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-25 DOI: 10.1039/D4SD00063C

Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest M. Kievit and Anthony J. Convertine

{"title":"Optimized gadolinium-DO3A loading in RAFT-polymerized copolymers for superior MR imaging of aging blood–brain barrier†","authors":"Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest M. Kievit and Anthony J. Convertine","doi":"10.1039/D4SD00063C","DOIUrl":"10.1039/D4SD00063C","url":null,"abstract":"The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. The resulting copolymers were analysed for their relaxivity properties, indicating that specific gadolinium-DO3A loading contents between 12–30 mole percent yield optimal MRI contrast enhancement. Inductively coupled plasma (ICP) measurements corroborated these findings, revealing a non-linear relationship between gadolinium content and relaxivity. Optimized copolymers were synthesized with the claudin-1 targeting peptide, C1C2, to image BBB targeting in aged mice to show imaging utility. This study presents a promising pathway for the development of more efficient GBCA addition to copolymers for targeted drug delivery and bioimaging application.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1513-1521"},"PeriodicalIF":3.5,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00063c?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141780330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper-based sensing of pancreatic-cancer biomarker α-chymotrypsin through turn-on lanthanide-luminescence† 基于纸张的胰腺癌生物标记物 α-Chymotrypsin 触发镧系元素发光传感技术

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-18 DOI: 10.1039/D4SD00124A

Ananya Biswas and Uday Maitra

引用次数: 0

An automated screening platform for improving the responsiveness of genetically encoded Ca2+ biosensors in mammalian cells† 提高哺乳动物细胞中基因编码 Ca2+ 生物传感器响应性的自动筛选平台

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-18 DOI: 10.1039/D4SD00138A

Yufeng Zhao, Yi Shen, Teodor Veres and Robert E. Campbell

{"title":"An automated screening platform for improving the responsiveness of genetically encoded Ca2+ biosensors in mammalian cells†","authors":"Yufeng Zhao, Yi Shen, Teodor Veres and Robert E. Campbell","doi":"10.1039/D4SD00138A","DOIUrl":"10.1039/D4SD00138A","url":null,"abstract":"Genetically-encoded, fluorescent protein (FP)-based biosensors are powerful tools for imaging dynamic cellular activities. Directed evolution is a highly effective method for developing enhanced versions of FP-based biosensors, but the screening process is laborious and time-consuming. Mammalian cell-based screening with electrical stimulation methods has been successful in accurately selecting variants of biosensors for imaging neuronal activities. We introduce an automated mammalian cell screening platform utilizing a fluorescence microscope and a liquid dispenser to enable the screening of biosensor responsiveness to chemical stimulation. We demonstrated the effectiveness of this platform in improving the response of a red fluorescent biosensor for Ca2+, K-GECO, for detection of histamine-induced changes in Ca2+ concentration. This method should be applicable to any FP-based biosensor that responds to pharmacological treatment or other exogenous chemical stimulation, simplifying efforts to develop biosensors tailored for specific applications in diverse biological contexts.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 9","pages":" 1494-1504"},"PeriodicalIF":3.5,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00138a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141743525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visually distinguishing between tumor tissue and healthy tissue within ten minutes using proteolytic probes† 利用蛋白水解探针在十分钟内目测区分肿瘤组织和健康组织

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-17 DOI: 10.1039/D4SD00047A

Debora Reinhardt, Björn ter Mors, Marc D. Driessen, Marcus Gutmann, Julian Faber, Lukas Haug, Anna-Maria Faber, Anna Herrmann, Prisca Hamm, Tessa Lühmann, Christian Linz and Lorenz Meinel

引用次数: 0

CRISPR/Cas13a-assisted amplification-free miRNA biosensor via dark-field imaging and magnetic gold nanoparticles† 通过暗场成像和磁性金纳米粒子实现 CRISPR/Cas13a 辅助的无扩增 miRNA 生物传感器

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-11 DOI: 10.1039/D4SD00081A

Jae-Jun Kim, Jae-Sang Hong, Hyunho Kim, Moonhyun Choi, Ursula Winter, Hakho Lee and Hyungsoon Im

{"title":"CRISPR/Cas13a-assisted amplification-free miRNA biosensor via dark-field imaging and magnetic gold nanoparticles†","authors":"Jae-Jun Kim, Jae-Sang Hong, Hyunho Kim, Moonhyun Choi, Ursula Winter, Hakho Lee and Hyungsoon Im","doi":"10.1039/D4SD00081A","DOIUrl":"10.1039/D4SD00081A","url":null,"abstract":"MicroRNAs (miRNAs) are short (about 18–24 nucleotides) non-coding RNAs and have emerged as potential biomarkers for various diseases, including cancers. Due to their short lengths, the specificity often becomes an issue in conventional amplification-based methods. Next-generation sequencing techniques could be an alternative, but the long analysis time and expensive costs make them less suitable for routine clinical diagnosis. Therefore, it is essential to develop a rapid, selective, and accurate miRNA detection assay using a simple, affordable system. In this work, we report a CRISPR/Cas13a-based miRNA biosensing using point-of-care dark-field (DF) imaging. We utilized magnetic-gold nanoparticle (MGNPs) complexes as signal probes, which consist of 200 nm-sized magnetic beads and 60 nm-sized gold nanoparticles (AuNPs) linked by DNA hybridization. Once the CRISPR/Cas13a system recognized the target miRNAs (miR-21-5p), the activated Cas13a cleaved the bridge linker containing RNA sequences, releasing 60 nm-AuNPs detected and quantified by a portable DF imaging system. The combination of CRISPR/Cas13a, MGNPs, and DF imaging demonstrated amplification-free detection of miR-21-5p within 30 min at a detection limit of 500 attomoles (25 pM) and with single-base specificity. The CRISPR/Cas13a-assisted MGNP-DF assay achieved rapid, selective, and accurate detection of miRNAs with simple equipment, thus providing a potential application for cancer diagnosis.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 8","pages":" 1310-1318"},"PeriodicalIF":3.5,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00081a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141587095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A holistic pathway to biosensor translation 生物传感器转化的整体途径

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-11 DOI: 10.1039/D4SD00088A

Laena D'Alton, Dênio Emanuel Pires Souto, Chamindie Punyadeera, Brian Abbey, Nicolas H. Voelcker, Conor Hogan and Saimon M. Silva

引用次数: 0

Cu2+-integrated carbon dots as an efficient bioprobe for the selective sensing of guanine nucleobase† Cu2+ 集成碳点作为选择性感知鸟嘌呤核碱基的高效生物探针

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-09 DOI: 10.1039/D4SD00137K

Monalisa Chowdhury, Debolina Basu and Prasanta Kumar Das

{"title":"Cu2+-integrated carbon dots as an efficient bioprobe for the selective sensing of guanine nucleobase†","authors":"Monalisa Chowdhury, Debolina Basu and Prasanta Kumar Das","doi":"10.1039/D4SD00137K","DOIUrl":"10.1039/D4SD00137K","url":null,"abstract":"This present work aimed to craft copper (Cu2+)-doped carbon dots (CuCDs) for the selective and sensitive detection of a guanine nucleobase. By employing a hydrothermal method, we synthesized blue-emitting CuCDs having emission maxima at 423 nm. CuCDs were used as a fluorescence turn-on ratiometric probe to detect guanine, a critical purine base in DNA involved in energy transduction, cell signalling, and metabolic processes. In the presence of guanine, the fluorescence intensity of CuCDs significantly increased due to the stable non-covalent interaction between Cu2+ and guanine. CuCDs achieved a very low limit of detection (LOD) of 0.59 nM for guanine as a highly sensitive probe. CuCDs demonstrated selectivity for guanine with no interference from other nucleobases (adenine, thymine, and cytosine) and various biomolecules and metal ions commonly found in the cellular environment. In addition, CuCDs demonstrated a higher affinity for guanine-enriched oligonucleotide cMYC G 27-mer over dsDNA 26-mer devoid of a large guanine population. Furthermore, the fluorescence intensity of CuCDs increased in guanine-treated mammalian cells and G-quadruplex-enriched cancer cells compared with that in non-cancerous cells. Hence, we developed a highly sensitive ratiometric fluorescence probe, CuCDs, for the selective detection of guanine both in vitro and within mammalian cells via a “fluorescence turn-on mechanism”.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 8","pages":" 1329-1343"},"PeriodicalIF":3.5,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00137k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141574612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic measurement of intracellular mRNA with a molecular beacon probe towards point-of-care radiation triage† 利用分子信标探针对细胞内 mRNA 进行微流控测量，实现医护点辐射分诊

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-02 DOI: 10.1039/D4SD00079J

Xin Meng, Kechun Wen, Jingyang Zhao, Yaru Han, Shanaz A. Ghandhi, Salan P. Kaur, David J. Brenner, Helen C. Turner, Sally A. Amundson and Qiao Lin

{"title":"Microfluidic measurement of intracellular mRNA with a molecular beacon probe towards point-of-care radiation triage†","authors":"Xin Meng, Kechun Wen, Jingyang Zhao, Yaru Han, Shanaz A. Ghandhi, Salan P. Kaur, David J. Brenner, Helen C. Turner, Sally A. Amundson and Qiao Lin","doi":"10.1039/D4SD00079J","DOIUrl":"10.1039/D4SD00079J","url":null,"abstract":"In large-scale radiation exposure events, the ability to triage potential victims by the received radiation dosage is crucial. This can be evaluated by radiation-induced biological changes. Radiation-responsive mRNA is a class of biomarkers that has been explored for dose-dependency with methods such as RT-qPCR. However, these methods are challenging to implement for point-of-care devices. We have designed and used molecular beacons as probes for the measurement of radiation-induced changes of intracellular mRNA in a microfluidic device towards determining radiation dosage. Our experiments, in which fixed TK6 cells labeled with a molecular beacon specific to BAX mRNA exhibited dose-dependent fluorescence in a manner consistent with RT-qPCR analysis, demonstrate that such intracellular molecular probes can potentially be used in point-of-care radiation biodosimetry. This proof of concept could readily be extended to any RNA-based test to provide direct measurements at the bedside.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 8","pages":" 1344-1352"},"PeriodicalIF":3.5,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00079j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and accurate identification of pathogenic bacteria using excitation–emission spectroscopy and machine learning† 利用激发-发射光谱和机器学习快速准确地识别病原菌

IF 3.5

Sensors & diagnostics Pub Date : 2024-07-02 DOI: 10.1039/D4SD00070F

Jacob Henry, Jennifer L. Endres, Marat R. Sadykov, Kenneth W. Bayles and Denis Svechkarev

{"title":"Fast and accurate identification of pathogenic bacteria using excitation–emission spectroscopy and machine learning†","authors":"Jacob Henry, Jennifer L. Endres, Marat R. Sadykov, Kenneth W. Bayles and Denis Svechkarev","doi":"10.1039/D4SD00070F","DOIUrl":"10.1039/D4SD00070F","url":null,"abstract":"Fast and reliable identification of pathogenic bacteria is of upmost importance to human health and safety. Methods that are currently used in clinical practice are often time consuming, require expensive equipment, trained personnel, and therefore have limited applications in low resource environments. Molecular identification methods address some of these shortcomings. At the same time, they often use antibodies, their fragments, or other biomolecules as recognition units, which makes such tests specific to a particular target. In contrast, array-based methods use a combination of reporters that are not specific to a single pathogen. These methods provide a more data-rich and universal response that can be used for identification of a variety of bacteria of interest. In this report, we demonstrate the application of the excitation–emission spectroscopy of an environmentally sensitive fluorescent dye for identification of pathogenic bacterial species. 2-(4′-Dimethylamino)-3-hydroxyflavone (DMAF) interacts with the bacterial cell envelope resulting in a distinct spectral response that is unique to each bacterial species. The dynamics of dye–bacteria interaction were thoroughly investigated, and the limits of detection and identification were determined. Neural network classification algorithm was used for pattern recognition analysis and classification of spectral data. The sensor successfully discriminated between eight representative pathogenic bacteria, achieving a classification accuracy of 85.8% at the species level and 98.3% at the Gram status level. The proposed method based on excitation–emission spectroscopy of an environmentally sensitive fluorescent dye is a powerful and versatile diagnostic tool with high accuracy in identification of bacterial pathogens.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":" 8","pages":" 1253-1262"},"PeriodicalIF":3.5,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00070f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0