Sensors & diagnostics最新文献_第9页

Aggregation-induced emission-active azines for chemosensing applications: a five-year update 用于化学传感应用的聚集诱导发射活性偶氮：五年更新

Sensors & diagnostics Pub Date : 2024-04-11 DOI: 10.1039/D3SD00348E

Akhil A. Bhosle, Mainak Banerjee and Amrita Chatterjee

{"title":"Aggregation-induced emission-active azines for chemosensing applications: a five-year update","authors":"Akhil A. Bhosle, Mainak Banerjee and Amrita Chatterjee","doi":"10.1039/D3SD00348E","DOIUrl":"10.1039/D3SD00348E","url":null,"abstract":"Azines are an important class of compounds that display solvent-dependent fluorescence emission depending upon the substituents in the aromatic scaffolds. They are affordable, easy to synthesize, stable, and well-suited for numerous applications. Unlike most other AIE fluorophores, aromatic units of AIE-active azine derivatives are bridged by rotatable N–N bonds rather than C–C bonds. Their derivatives have been widely used in pharmaceuticals, drug delivery, organometallics, optoelectronics, dyes, etc., and most importantly in several sensing applications. This comprehensive review encapsulates the recent developments in the field of AIE-active azine molecules and their applications as chemosensors in the detection of various analytes. The review discusses the different chemosensing strategies involved in the detection of metal ions (Cu2+, Zn2+, Al3+, Fe3+, Cr3+, Hg2+, UO22+, etc.), anions (F−, CN−, ClO−, ONOO−, HSO3−), small molecules (thiols, hydrazine, hydrogen peroxide), and bio-analytes (protamine/heparin, HSA/BSA, neuraminidase, β-lactamase, β-galactosidase, etc.) with a focus on the development in the last five years. The review also highlights the advancements in azine-based systems for their use in imaging, supramolecular host–guest recognitions, AIE polymers, COFs/MOFs, etc.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d3sd00348e?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-scale validation of a plasmonic sensor for SARS-CoV-2 pseudo-neutralization with a cohort of food and retail workers† 利用食品和零售业工人群体大规模验证 SARS-CoV-2 伪中性的质子传感器

Sensors & diagnostics Pub Date : 2024-04-09 DOI: 10.1039/D3SD00333G

Julien Coutu, Pierre Ricard, Abdelhadi Djaïleb, Étienne Lavallée, Henintsoa Rabezanahary, Matthew Stuible, Yves Durocher, Caroline Gilbert, Nicholas Brousseau, Kim Santerre, Mathieu Thériault, Sylvie Trottier, Denis Boudreau, Marc-André Langlois, Joelle N. Pelletier, Mariana Baz and Jean-Francois Masson

{"title":"Large-scale validation of a plasmonic sensor for SARS-CoV-2 pseudo-neutralization with a cohort of food and retail workers†","authors":"Julien Coutu, Pierre Ricard, Abdelhadi Djaïleb, Étienne Lavallée, Henintsoa Rabezanahary, Matthew Stuible, Yves Durocher, Caroline Gilbert, Nicholas Brousseau, Kim Santerre, Mathieu Thériault, Sylvie Trottier, Denis Boudreau, Marc-André Langlois, Joelle N. Pelletier, Mariana Baz and Jean-Francois Masson","doi":"10.1039/D3SD00333G","DOIUrl":"10.1039/D3SD00333G","url":null,"abstract":"Plasmonic sensors are candidates for numerous clinical applications, but few examples demonstrate their performance on large sample cohorts, a necessary step for clinical translation. The COVID-19 pandemic provided an unprecedented opportunity to validate a surface plasmon resonance (SPR) sensor for SARS-CoV-2 inhibition with a cohort of over 1000 clinical samples from the longitudinal study of a food and retail worker population. The SPR sensor provided an in vitro model to assess the level of neutralizing antibodies by measuring the inhibition of the SARS-CoV-2 spike protein interaction with ACE-2 following exposure of the spike protein to naive and immune sera (from vaccination and/or infection). In conjunction with population data on vaccination and infection, and epidemiological data from the local jurisdiction of the study cohort, it is shown that the SPR sensor performed well in assessing the level of “pseudo-neutralization” of participant sera and that the response of the SPR sensor correlates (r = 0.74) with a live virus microneutralization assay as well as with metadata of relevant events (vaccination, waves of infection, etc.) that occurred during the study period. Using these data, the article details the challenges and opportunities of using plasmonic sensors in clinical practice.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d3sd00333g?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fiber-optic probes for real-time pH monitoring† 用于实时 pH 值监测的光纤探头

Sensors & diagnostics Pub Date : 2024-04-08 DOI: 10.1039/D4SD00012A

Mohamed Elsherif, Fahad Alam, Ahmed E. Salih, Xinyu Wang, Peter R. Corridon, Khalil B. Ramadi and Haider Butt

{"title":"Fiber-optic probes for real-time pH monitoring†","authors":"Mohamed Elsherif, Fahad Alam, Ahmed E. Salih, Xinyu Wang, Peter R. Corridon, Khalil B. Ramadi and Haider Butt","doi":"10.1039/D4SD00012A","DOIUrl":"10.1039/D4SD00012A","url":null,"abstract":"While pH determination is a commonplace laboratory practice, conventional commercial pH probes exhibit drawbacks of bulkiness, slow response times, and signal drift. These become particularly limiting in specialized fields like tissue engineering and bio-industrial processing, where unique pH probe specifications surpass the capabilities of standard laboratory equipment. Here, we present the development of compact pH fiber probes by integrating silica optical fiber with a colorimetric pH indicator. Our approach involves cross-linking the pH indicator with a biocompatible synthetic hydrogel matrix, facilitating colorimetric and precise pH measurements. Two distinct designs of optical fiber sensors were devised to cater to a broad spectrum of applications. The first design involved attaching the hydrogel sensor to the fiber tip during the photopolymerization process, while the second design was crafted by folding the hydrogel sensor onto the bare terminal of the fiber using the casting process. The fiber sensor exhibited high sensitivity (17 nm pH−1) within physiological and pathophysiological pH ranges (6–8) when tested in reflection configuration. Validation of the developed fiber sensors was carried out on cancerous tissue phantoms derived from an ovine extracellular matrix. The unique specifications of these fiber sensors position them as promising candidates for applications in tissue engineering, cell growth, and continuous blood pH monitoring.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00012a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-powered triboelectric nanogenerator with enhanced surface charge density for dynamic multidirectional pressure sensing 具有增强表面电荷密度的自供电三电纳米发电机，用于动态多向压力传感

Sensors & diagnostics Pub Date : 2024-04-02 DOI: 10.1039/d4sd00019f

Jiaqi Wu, Yu Zhang, Xin Ting Zheng

{"title":"Self-powered triboelectric nanogenerator with enhanced surface charge density for dynamic multidirectional pressure sensing","authors":"Jiaqi Wu, Yu Zhang, Xin Ting Zheng","doi":"10.1039/d4sd00019f","DOIUrl":"https://doi.org/10.1039/d4sd00019f","url":null,"abstract":"In the rapidly evolving landscape of the internet of things (IoT) and the burgeoning field of biomedical applications, development in human–machine interfaces and human motion monitoring has accentuated the need for real-time pressure sensing. However, the challenge of developing a sensor that combines high sensitivity in the low pressure range with real-time remote sensing capability has remained a significant obstacle in both fields. Herein, a self-powered triboelectric nanogenerator (TENG) based pressure sensor (STEPs) with real-time remote sensing ability is proposed to meet these critical demands, offering high sensitivity in the range of 0–70 mmHg, catering to the needs of human–machine interface and biomedical applications. The STEPs introduces an innovative composite material, blending polydimethylsiloxane (PDMS), carbon black (CB), and polyvinylpyrrolidone (PVP) for improved sensing performance, then this is subjected to ultrasonication and degassing to ensure homogeneous dispersion. The doping of CB and PVP at optimal percentages into the PDMS matrix of the STEPs, together with a unique three-dimensional structure of the sensor, achieves an optimized surface charge density, leading to a high sensitivity 2.61 ± 0.02 mV mmHg−1, as compared with previous works. A wireless measurement and data transfer system, established between a STEPs array for multidirectional pressure sensing and a remote readout device following the Transmission Control Protocol (TCP), further enables real-time remote display of pressure readings. This research underscores the novelty and broad applicability of this sensor, with the potential to revolutionize self-powered wearable sensors in both human–machine interface and biomedical applications.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible & transparent breath sensor and conducting electrodes based on a highly interconnected Au nanoparticle network 基于高度互联金纳米粒子网络的柔性透明呼吸传感器和导电电极

Sensors & diagnostics Pub Date : 2024-03-28 DOI: 10.1039/d3sd00330b

Namuni Sneha, S. Kiruthika

{"title":"Flexible & transparent breath sensor and conducting electrodes based on a highly interconnected Au nanoparticle network","authors":"Namuni Sneha, S. Kiruthika","doi":"10.1039/d3sd00330b","DOIUrl":"https://doi.org/10.1039/d3sd00330b","url":null,"abstract":"In this study, transparent humidity sensors and conducting electrodes (TCEs) were fabricated using a highly interconnected gold nanoparticle network (Au nanonetwork) via a simple solution approach. Here, the direct reduction of metal anions at lower temperatures (5 °C) followed by the addition of a non-polar solvent facilitates the cold welding of nanoparticles at junctions and yields an interconnected Au nanonetwork at the liquid–liquid interface. The formed Au nanonetwork is cautiously transferred to the desired flexible, stretchable, and transparent substrates for various applications. As the synthesis involves no capping agents, the prepared nanonetworks offer high conductivity without further chemical or thermal treatments. The fabricated Au nanonetwork is highly crystalline and thermally stable, with excellent mechanical robustness towards various deformations. Using an Au-1L (1-layer) nanonetwork, a highly transparent (>85%) humidity sensor is fabricated with short response and recovery times (1.1 s and 1.3 s). The Au-1L sensor is studied systematically for various humidity changes (40% to 90%) and breath conditions (normal/deep, hydrated/dehydrated, breathing/blowing, etc.) and exhibits high selectivity toward humidity. Notably, the sensing device offered a stable response for more than a year demonstrating its robustness for prolonged use.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140316193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved point-of-care detection of P. gingivalis using optimized surface-enhanced Raman scattering in lateral flow assays† 利用优化的表面增强拉曼散射技术在侧流检测中改进牙龈脓疱病的护理点检测

Sensors & diagnostics Pub Date : 2024-03-28 DOI: 10.1039/D4SD00056K

Lyndsay N. Kissell, Daewoo Han, Der Vang, Alexander W. R. Cikanek, Andrew J. Steckl and Pietro Strobbia

{"title":"Improved point-of-care detection of P. gingivalis using optimized surface-enhanced Raman scattering in lateral flow assays†","authors":"Lyndsay N. Kissell, Daewoo Han, Der Vang, Alexander W. R. Cikanek, Andrew J. Steckl and Pietro Strobbia","doi":"10.1039/D4SD00056K","DOIUrl":"10.1039/D4SD00056K","url":null,"abstract":"The introduction of surface-enhanced Raman scattering (SERS) in lateral flow assays (LFA) has been recently exploited to increase the sensitivity and quantification capabilities of these rapid tests. Herein, we took advantage of a SERS–LFA combination to improve LFAs designed to test for P. gingivalis, a biomarker for oral health. We have demonstrated a limit of detection (LOD) < 10 ng mL−1, which is within the range of concentration needed to monitor oral health. By comparison, conventional colorimetric LFAs achieve an LOD ≈ 100 ng mL−1, generating a lower diagnostic sensitivity. To achieve this enhanced sensitivity, we optimized the materials used in the SERS–LFA, investigating nanostars (NS) differing in size and material composition and comparing them to commercial gold nanoparticles, as a benchmark. We found that large (56 nm) NSs with a silver coating were the most sensitive nanomaterials for SERS–LFA. To prove the applicability of this SERS–LFA to point-of-care (POC) settings, we tested the optimized LFA with a portable Raman system prototype designed to work on LFAs with 3D-printed cartridges and a line-shaped laser illumination. Using this prototype, we achieved the same LOD observed with the traditional benchtop Raman system. The use of a portable Raman system has brought the SERS–LFA technology closer to the POC use.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00056k?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140324723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The integrated on-chip isolation and detection of circulating tumour cells 片上分离和检测循环肿瘤细胞的集成方法

Sensors & diagnostics Pub Date : 2024-03-26 DOI: 10.1039/d3sd00302g

Sophia Marie Abusamra, Robert Barber, Mohamed Sharafeldin, Claire M. Edwards, Jason J Davis

{"title":"The integrated on-chip isolation and detection of circulating tumour cells","authors":"Sophia Marie Abusamra, Robert Barber, Mohamed Sharafeldin, Claire M. Edwards, Jason J Davis","doi":"10.1039/d3sd00302g","DOIUrl":"https://doi.org/10.1039/d3sd00302g","url":null,"abstract":"Circulating tumour cells (CTCs) are cancer cells shed from a primary tumour which intravasate into the blood stream and have the potential to extravasate into distant tissues, seeding metastatic lesions. As such, they can offer important insight into cancer progression with their presence generally associated with a poor prognosis. The detection and enumeration of CTCs is, therefore, critical to guiding clinical decisions during treatment and providing information on disease state. CTC isolation has been investigated using a plethora of methodologies, of which immunomagnetic capture and microfluidic size-based filtration are the most impactful to date. However, the isolation and detection of CTCs from whole blood comes with many technical barriers, such as those presented by the phenotypic heterogeneity of cell surface markers, morphological similarity to healthy blood cells, and their low relative abundance (~1 CTC/1 billion blood cells). At present, the majority of reported methods dissociate CTC isolation from detection, a workflow which undoubtedly contributes to loss from an already sparse population. This review focuses on developments wherein isolation and detection have been integrated into a single-step, microfluidic configuration, reducing CTC loss, increasing throughput, and enabling an on-chip CTC analysis with minimal operator intervention. Particular attention is given to immune-affinity, microfluidic CTC isolation, coupled to optical, physical, and electrochemical CTC detection (quantitative or otherwise).","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140316034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivity-based amino-1,8-naphthalimide fluorescent chemosensors for the detection and monitoring of phosgene 基于反应性的氨基-1,8-萘二甲酰亚胺荧光化学传感器用于检测和监测光气

Sensors & diagnostics Pub Date : 2024-03-26 DOI: 10.1039/D4SD00048J

Mannanthara Kunhumon Noushija, Alenthwar Vamshi Krishna, Thorfinnur Gunnlaugsson and Sankarasekaran Shanmugaraju

{"title":"Reactivity-based amino-1,8-naphthalimide fluorescent chemosensors for the detection and monitoring of phosgene","authors":"Mannanthara Kunhumon Noushija, Alenthwar Vamshi Krishna, Thorfinnur Gunnlaugsson and Sankarasekaran Shanmugaraju","doi":"10.1039/D4SD00048J","DOIUrl":"10.1039/D4SD00048J","url":null,"abstract":"Phosgene (carbonyl dichloride, COCl2) is an extremely toxic and hazardous chemical warfare agent (CWA; schedule 3 substance) that poses significant threats to public safety and human well-being. Simultaneously, it is a commonly employed reagent in chemical synthesis in laboratories and industrial settings. Therefore, monitoring phosgene concentration levels is essential for handling them within the workplace and for safeguarding public security. Activity-based fluorescent probes are the most effective real-time detection methods currently used for the detection of CWAs. Reaction-based sensing offers excellent temporal and spatial resolution, minimal side effects, and quick response times. Recently, a plethora of reaction-based fluorescent probes have been designed for the detection of phosgene. This review provides an overview of the latest developments using amino-1,8-naphthalimide-based small-molecule fluorescent probes designed for phosgene detection/sensing. Additionally, we investigate the existing challenges and prospects in the field of reaction-based fluorescent probes for phosgene detection. Herein, various fluorescence sensors are categorized based on their reactions with phosgene, and each section highlights the reaction sites, sensing mechanisms, structure–function relationships, photophysical performances, and practical applications of these sensors. It is our aspiration that this review will provide valuable insights into the advancement of cutting-edge fluorescent probes tailored for phosgene detection and sensing.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d4sd00048j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140300783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Analyte Size on SERS Enhancement Location, Enhancement Factor, Excitation Wavelength, and Spectrum 分析物尺寸对 SERS 增强位置、增强因子、激发波长和光谱的影响

Sensors & diagnostics Pub Date : 2024-03-22 DOI: 10.1039/d4sd00014e

Yanjun Yang, Xinyi Chen, Bin Ai, Yiping Zhao

{"title":"The Impact of Analyte Size on SERS Enhancement Location, Enhancement Factor, Excitation Wavelength, and Spectrum","authors":"Yanjun Yang, Xinyi Chen, Bin Ai, Yiping Zhao","doi":"10.1039/d4sd00014e","DOIUrl":"https://doi.org/10.1039/d4sd00014e","url":null,"abstract":"The study systematically explores the connection between analyte particle size and the hot-spot in Au nanoparticle (NP) dimer systems. Contrary to the conventional understanding tied to localized surface plasmon resonance (LSPR), we show that depending on the analyte particle’s size, the location to produce surface-enhanced Raman scattering (SERS), defined as effective hot-spot, is different from the gap based hot-spot, where the electric field reaches maximum intensity, and the corresponding resonant wavelength is also shifted significantly from LSPR wavelength. This effective hot-spot occurs primarily at the point where the Au NP contacts the analyte particle, covering a larger area than the traditional hotspot and having a significantly smaller enhancement factor. Moreover, different effective hot-spots can be activated under various polarizations. The local electric field versus distance relationship decays significantly slower, complicating the interpretation of SERS spectra of large analyte particles. This complexity offers tunability, allowing for a more precise representation of unique molecular features of the analyte. Consequently, our findings demonstrate the necessity for SERS substrate design rules to be contingent on analyte particle size. Although interpreting SERS spectra is intricate, it can be refined to effectively capture distinctive molecular characteristics. These insights pave a new way to tailor SERS substrate design specifically catering to large analyte particles.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140198780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An approach to use machine learning to optimize paper immunoassays for SARS-CoV-2 IgG and IgM antibodies 利用机器学习优化 SARS-CoV-2 IgG 和 IgM 抗体纸质免疫测定的方法

Sensors & diagnostics Pub Date : 2024-03-15 DOI: 10.1039/d3sd00327b

Josselyn Mata Calidonio, Kimberly Hamad-Schifferli

{"title":"An approach to use machine learning to optimize paper immunoassays for SARS-CoV-2 IgG and IgM antibodies","authors":"Josselyn Mata Calidonio, Kimberly Hamad-Schifferli","doi":"10.1039/d3sd00327b","DOIUrl":"https://doi.org/10.1039/d3sd00327b","url":null,"abstract":"We developed a COVID-19 paper immunoassay that can detect IgG and IgM antibodies for both SARS CoV-2 spike and nucleocapsid proteins. The test is a multicolor assay that uses as the label gold nanoparticles of different shapes (spheres vs. stars), which result in visually distinct colors of red and blue at the test areas. The assay uses spike and nucleocapsid pro-teins immobilized on nitrocellulose strip at different locations, and the nanoparticle-antibody conjugates run are red nanopar-ticles conjugated to anti-IgG, and blue nanostars conjugated to anti-IgM. Spatial location of signal indicates whether the antibody present is anti-spike or an anti-nucleocapsid, and test area color indicates which antibody type (IgG vs. IgM). Line-ar discriminant analysis (LDA) and machine learning (ML) were used to evaluate the test accuracy, and then used iteratively to modify the assay running conditions (present of quencher molecules, nanoparticle types, washes,) until the test accuracy reached 100 %. The resulting assay could distinguish between 9 different antibody profiles indicative of different disease cases (prior infection vs. vaccinated, early/mid/late stage post infection). Results show that expanding test beyond a specific sensing to selective sensing as an array can enable rapid immunoassays to obtain more complex information, and supervised learning can accelerate test development.","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140155193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0