Visually distinguishing between tumor tissue and healthy tissue within ten minutes using proteolytic probes†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Debora Reinhardt, Björn ter Mors, Marc D. Driessen, Marcus Gutmann, Julian Faber, Lukas Haug, Anna-Maria Faber, Anna Herrmann, Prisca Hamm, Tessa Lühmann, Christian Linz and Lorenz Meinel
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引用次数: 0

Abstract

Accurately identifying tumor tissue is crucial during surgery, especially when removing head and neck squamous cell carcinomas (HNSCC). Our tumor-responsive probes are tailored for ex vivo diagnostics, streamlining today's complex surgical workflows and potentially enabling pathologists and surgeons to rapidly and objectively distinguish between healthy and tumor tissue. Designed based on insights from biological furin substrates and cleavage site screening, the probes detect HNSCC-associated protease activity. Within ten minutes of incubation, tumor tissue is differentiated from healthy tissue by visible fluorescence in biopsy supernatant.

Abstract Image

Abstract Image

利用蛋白水解探针在十分钟内目测区分肿瘤组织和健康组织
在手术过程中,尤其是切除头颈部鳞状细胞癌 (HNSCC) 时,准确识别肿瘤组织至关重要。我们的肿瘤反应探针专为体内外诊断量身定制,可简化当今复杂的手术工作流程,使病理学家和外科医生能够快速、客观地区分健康组织和肿瘤组织。探针的设计基于对生物呋喃底物和裂解位点筛选的深入了解,可检测 HNSCC 相关蛋白酶的活性。在孵育十分钟内,肿瘤组织就能通过活检上清液中的可见荧光与健康组织区分开来。
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来源期刊
CiteScore
2.30
自引率
0.00%
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