PHAGE (New Rochelle, N.Y.)最新文献

{"title":"Novel <i>Escherichia coli</i>-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates.","authors":"Janet Yakubu Nale,&nbsp;Benjamin Chan,&nbsp;Nnaemeka Emmanuel Nnadi,&nbsp;Jeffrey Kwok Jone Cheng,&nbsp;Susan Matts,&nbsp;Neda Nezam-Abadi,&nbsp;Christopher Jason Richard Turkington,&nbsp;Lucie Manon Charreton,&nbsp;Harroop Bola,&nbsp;Ramez Nazir,&nbsp;Abubakar Shaaban Hoza,&nbsp;Samuel Posian Wamala,&nbsp;Ivan Ibanda,&nbsp;Alice Nyambura Maina,&nbsp;Auleria Ajiambo Apopo,&nbsp;Venance Theophil Msoffe,&nbsp;Nyambura Moremi,&nbsp;Grace Wanjiru Moore,&nbsp;Ismail Asiimwe,&nbsp;Alice Namatovu,&nbsp;Paul Mutumba,&nbsp;Deus Kamya,&nbsp;Ritah Nabunje,&nbsp;Immaculate Nakabugo,&nbsp;Rudovick Ruben Kazwala,&nbsp;Erastus Kangethe,&nbsp;Abel Abera Negash,&nbsp;Arthur Kalyebi Watelo,&nbsp;Nelson Bukamba,&nbsp;Gideon Muhindo,&nbsp;Nathan Musisi Lubowa,&nbsp;Ngalla Jillani,&nbsp;Atunga Nyachieo,&nbsp;George Nasinyama,&nbsp;Jesca Nakavuma,&nbsp;Andrew Millard,&nbsp;Tobi Elaine Nagel,&nbsp;Martha Rebecca Jane Clokie","doi":"10.1089/phage.2023.0012","DOIUrl":"10.1089/phage.2023.0012","url":null,"abstract":"<p><strong>Background: </strong>The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.</p><p><strong>Materials and methods: </strong>During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel <i>Escherichia coli</i> lytic phages were isolated and characterized.</p><p><strong>Results: </strong>The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera <i>Dhakavirus</i> and <i>Tunavirus</i>, respectively.</p><p><strong>Conclusion: </strong>The phages isolated have therapeutic potential for further development against <i>E. coli</i> infections.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"141-149"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574529/pdf/phage.2023.0012.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHAGE Impact and the Impact of Phages.","authors":"Martha Clokie,&nbsp;Thomas Sicheritz-Pontén","doi":"10.1089/phage.2023.29046.editorial","DOIUrl":"10.1089/phage.2023.29046.editorial","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"110"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574526/pdf/phage.2023.29046.editorial.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cooling waters: Phages to treat urinary tract infections-Phage Cartoon by Ellie Jameson.","authors":"","doi":"10.1089/phage.2023.29045.car","DOIUrl":"10.1089/phage.2023.29045.car","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"111"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574528/pdf/phage.2023.29045.car.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Correction to: In Vitro</i> Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol by Kok et al. <i>Phage (New Rochelle)</i> 2023;4(2):68-81. DOI: 10.1089/phage.2023.0005.","authors":"","doi":"10.1089/phage.2023.0005.correx","DOIUrl":"10.1089/phage.2023.0005.correx","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1089/phage.2023.0005.].</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"150"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574524/pdf/phage.2023.0005.correx.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.","authors":"Daniel W Bryan,&nbsp;Lauren K Hudson,&nbsp;Jia Wang,&nbsp;Thomas G Denes","doi":"10.1089/phage.2023.0004","DOIUrl":"https://doi.org/10.1089/phage.2023.0004","url":null,"abstract":"<p><strong>Background: </strong><i>Salmonella enterica</i> is one of the most prevalent bacterial foodborne pathogens. <i>Salmonella</i> phages are currently used in biocontrol applications and have potential for use as therapeutics.</p><p><strong>Materials and methods: </strong>Phages were enriched and purified from a diversity of <i>Salmonella</i> host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic analysis was performed to determine taxonomy.</p><p><strong>Results: </strong>Ten phages were isolated and characterized. Phages showed activity against 23 out of the 24 <i>Salmonella</i> serovars evaluated. Two phages also showed activity against <i>Escherichia coli</i> strain B. Phages belonged to five different genera (<i>Ithacavirus</i>, <i>Gelderlandvirus</i>, <i>Kuttervirus</i>, <i>Tlsvirus</i>, and <i>Epseptimavirus</i>), two established species, and eight novel species.</p><p><strong>Conclusions: </strong>The phages described here further demonstrate the diversity of <i>S. enterica</i> phages present in wastewater effluent. This work contributes a collection of characterized phages from eastern Tennessee that may be of use in future phage-based applications targeting <i>S. enterica</i>.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"90-98"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Can Play at That Game: Phage-Antibiotic Synergy-Phage Cartoon by Ellie Jameson.","authors":"","doi":"10.1089/phage.2023.29042.car","DOIUrl":"10.1089/phage.2023.29042.car","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"54"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10059530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Summer of Phage Conferences Reflects the Burgeoning Interest in Phages.","authors":"Martha R J Clokie, Thomas Sicheritz-Pontén","doi":"10.1089/phage.2023.29043.editorial","DOIUrl":"10.1089/phage.2023.29043.editorial","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"53"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10059531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol.","authors":"Danielle N Kok, Joanne Turnbull, Nobuto Takeuchi, Philippos K Tsourkas, Heather L Hendrickson","doi":"10.1089/phage.2023.0005","DOIUrl":"10.1089/phage.2023.0005","url":null,"abstract":"<p><strong>Background: </strong>Bacteriophages are becoming increasingly important in the race to find alternatives to antibiotics. Unfortunately, bacteriophages that might otherwise be useful are sometimes discarded due to low titers making them unsuitable for downstream applications.</p><p><strong>Methods: </strong>Here, we present two distinct approaches used to experimentally evolve novel New Zealand Paenibacillus larvae bacteriophages. The first approach uses the traditional agar-overlay method, whereas the other was a 96-well plate liquid infection protocol that improved phage titers in as little as four days. We also used a mathematical model to probe the parameters and limits of the RAMP-UP approach to rapidly select mutants that improve bacteriophage titers.</p><p><strong>Results: </strong>Both experimental approaches resulted in an increase in plaque-forming units (PFU/mL). The liquid infection approach developed here, which we call RAMP-UP for Rapid Adaptive Mutation of Phage - UP, was significantly faster and simpler, and allowed us to evolve high titer bacteriophages in as little as four days. Titers were increased from 100-100,000-fold relative to their ancestors. The resultant titers were sufficient to extract and sequence DNA from these bacteriophages. An analysis of these phage genomes is provided.</p><p><strong>Conclusion: </strong>The RAMP-UP protocol is an effective method for experimentally evolving previously intractable bacteriophages in a high-throughput and expeditious manner.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"68-81"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Two Is Better Than One\": The Multifactorial Nature of Phage-Antibiotic Combinatorial Treatments Against ESKAPE-Induced Infections.","authors":"Gale Bernice N Fungo, John Christian W Uy, Kristiana Louise J Porciuncula, Chiarah Mae A Candelario, Deneb Philip S Chua, Tracey Antaeus D Gutierrez, Martha Rebecca Jane Clokie, Donna May D Papa","doi":"10.1089/phage.2023.0007","DOIUrl":"10.1089/phage.2023.0007","url":null,"abstract":"<p><p>Phage-antibiotic synergy (PAS) has been extensively explored over the past decade, with the aim of developing more effective treatments against multidrug-resistant organisms. However, it remains unclear how to effectively combine these two approaches. To address this uncertainty, we assessed four main aspects of PAS interactions in this review, seeking to identify commonalities of combining treatments within and between bacterial species. We examined all literature on PAS efficacy toward ESKAPE pathogens and present an analysis of the data in papers focusing on: (1) order of treatment, (2) dose of both phage and antibiotics, (3) mechanism of action, and (4) viability of transfer from <i>in vivo</i> or animal model trials to clinical applications. Our analysis indicates that there is little consistency within phage-antibiotic therapy regimens, suggesting that highly individualized treatment regimens should be used. We propose a set of experimental studies to address these research gaps. We end our review with suggestions on how to improve studies on phage-antibiotic combination therapy to advance this field.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"55-67"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Phages That Bypass the Requirement for RNA-Mediated Antitermination.","authors":"Rodney A King, Millicent Babbs, Kimberly Baugh, Courtney Hamilton","doi":"10.1089/phage.2023.0008","DOIUrl":"10.1089/phage.2023.0008","url":null,"abstract":"<p><strong>Introduction: </strong>The <i>rpoC</i>Y75N mutation in the zinc-binding domain of the β' subunit of <i>Escherichia coli</i> RNA polymerase blocks the RNA-based mechanism of transcription antitermination utilized by bacteriophage HK022.</p><p><strong>Materials and methods: </strong>Mutant phages that overcome the block imposed by the <i>rpoC</i>Y75N mutation are described. These phages, designated \"<i>orc</i>\" (overcomes <i>rpoC</i>), carry mutations that create new promoters. Promoter activity was assessed by cloning the respective regions from the wild-type and <i>orc</i> phages into a promoterless <i>lacZ</i> reporter vector.</p><p><strong>Results: </strong>Reporter assays showed that the sequence originating from <i>orc</i> phages had significant promoter activity when compared with the equivalent sequence cloned from the parental phage.</p><p><strong>Conclusions: </strong>The newly created promoters facilitate the expression of phage genes that are essential for growth on the <i>rpoC</i>Y75N strain by bypassing transcription terminators. The small plaque phenotype of <i>orc</i> phages, when grown on the mutant host, suggests that suppression of the <i>rpoC</i>Y75N mutation is incomplete.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"82-89"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9710350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}