PHAGE (New Rochelle, N.Y.)最新文献_第4页

PHAGE Impact and the Impact of Phages. 噬菌体的影响和噬菌体的影响。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.29046.editorial

Martha Clokie, Thomas Sicheritz-Pontén

引用次数: 0

Cooling waters: Phages to treat urinary tract infections-Phage Cartoon by Ellie Jameson. 凉水：治疗尿路感染的噬菌体艾丽詹姆森的噬菌体漫画。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.29045.car

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Correction to: In Vitro Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol by Kok et al. Phage (New Rochelle) 2023;4(2):68-81. DOI: 10.1089/phage.2023.0005. 更正：体外进化以增加困难噬菌体的滴度：Kok等人的RAMP-UP方案。Phage（New Rochelle）2023；4（2）：68-81.DOI:10.1089/phage.2023.0005。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.0005.correx

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Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater. 从田纳西州废水中分离的多种噬菌体沙门氏菌的特性。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 DOI: 10.1089/phage.2023.0004

Daniel W Bryan, Lauren K Hudson, Jia Wang, Thomas G Denes

{"title":"Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.","authors":"Daniel W Bryan, Lauren K Hudson, Jia Wang, Thomas G Denes","doi":"10.1089/phage.2023.0004","DOIUrl":"https://doi.org/10.1089/phage.2023.0004","url":null,"abstract":"Background: Salmonella enterica is one of the most prevalent bacterial foodborne pathogens. Salmonella phages are currently used in biocontrol applications and have potential for use as therapeutics.Materials and methods: Phages were enriched and purified from a diversity of Salmonella host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic analysis was performed to determine taxonomy.Results: Ten phages were isolated and characterized. Phages showed activity against 23 out of the 24 Salmonella serovars evaluated. Two phages also showed activity against Escherichia coli strain B. Phages belonged to five different genera (Ithacavirus, Gelderlandvirus, Kuttervirus, Tlsvirus, and Epseptimavirus), two established species, and eight novel species.Conclusions: The phages described here further demonstrate the diversity of S. enterica phages present in wastewater effluent. This work contributes a collection of characterized phages from eastern Tennessee that may be of use in future phage-based applications targeting S. enterica.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"90-98"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Can Play at That Game: Phage-Antibiotic Synergy-Phage Cartoon by Ellie Jameson. 两个人也能玩的游戏噬菌体与抗生素的协同作用--噬菌体漫画》，作者艾莉-詹姆森。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 Epub Date: 2023-06-19 DOI: 10.1089/phage.2023.29042.car

引用次数: 0

A Summer of Phage Conferences Reflects the Burgeoning Interest in Phages. 夏季噬菌体会议反映了人们对噬菌体的浓厚兴趣。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 Epub Date: 2023-06-19 DOI: 10.1089/phage.2023.29043.editorial

Martha R J Clokie, Thomas Sicheritz-Pontén

引用次数: 0

In Vitro Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol. 体外进化以增加困难噬菌体的滴度：RAMP-UP方案。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 Epub Date: 2023-06-19 DOI: 10.1089/phage.2023.0005

Danielle N Kok, Joanne Turnbull, Nobuto Takeuchi, Philippos K Tsourkas, Heather L Hendrickson

{"title":"In Vitro Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol.","authors":"Danielle N Kok, Joanne Turnbull, Nobuto Takeuchi, Philippos K Tsourkas, Heather L Hendrickson","doi":"10.1089/phage.2023.0005","DOIUrl":"10.1089/phage.2023.0005","url":null,"abstract":"Background: Bacteriophages are becoming increasingly important in the race to find alternatives to antibiotics. Unfortunately, bacteriophages that might otherwise be useful are sometimes discarded due to low titers making them unsuitable for downstream applications.Methods: Here, we present two distinct approaches used to experimentally evolve novel New Zealand Paenibacillus larvae bacteriophages. The first approach uses the traditional agar-overlay method, whereas the other was a 96-well plate liquid infection protocol that improved phage titers in as little as four days. We also used a mathematical model to probe the parameters and limits of the RAMP-UP approach to rapidly select mutants that improve bacteriophage titers.Results: Both experimental approaches resulted in an increase in plaque-forming units (PFU/mL). The liquid infection approach developed here, which we call RAMP-UP for Rapid Adaptive Mutation of Phage - UP, was significantly faster and simpler, and allowed us to evolve high titer bacteriophages in as little as four days. Titers were increased from 100-100,000-fold relative to their ancestors. The resultant titers were sufficient to extract and sequence DNA from these bacteriophages. An analysis of these phage genomes is provided.Conclusion: The RAMP-UP protocol is an effective method for experimentally evolving previously intractable bacteriophages in a high-throughput and expeditious manner.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"68-81"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Two Is Better Than One": The Multifactorial Nature of Phage-Antibiotic Combinatorial Treatments Against ESKAPE-Induced Infections. "二胜于一"：噬菌体-抗生素联合疗法对抗 ESKAPE 感染的多因素性质。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 Epub Date: 2023-06-19 DOI: 10.1089/phage.2023.0007

Gale Bernice N Fungo, John Christian W Uy, Kristiana Louise J Porciuncula, Chiarah Mae A Candelario, Deneb Philip S Chua, Tracey Antaeus D Gutierrez, Martha Rebecca Jane Clokie, Donna May D Papa

{"title":"\"Two Is Better Than One\": The Multifactorial Nature of Phage-Antibiotic Combinatorial Treatments Against ESKAPE-Induced Infections.","authors":"Gale Bernice N Fungo, John Christian W Uy, Kristiana Louise J Porciuncula, Chiarah Mae A Candelario, Deneb Philip S Chua, Tracey Antaeus D Gutierrez, Martha Rebecca Jane Clokie, Donna May D Papa","doi":"10.1089/phage.2023.0007","DOIUrl":"10.1089/phage.2023.0007","url":null,"abstract":"Phage-antibiotic synergy (PAS) has been extensively explored over the past decade, with the aim of developing more effective treatments against multidrug-resistant organisms. However, it remains unclear how to effectively combine these two approaches. To address this uncertainty, we assessed four main aspects of PAS interactions in this review, seeking to identify commonalities of combining treatments within and between bacterial species. We examined all literature on PAS efficacy toward ESKAPE pathogens and present an analysis of the data in papers focusing on: (1) order of treatment, (2) dose of both phage and antibiotics, (3) mechanism of action, and (4) viability of transfer from in vivo or animal model trials to clinical applications. Our analysis indicates that there is little consistency within phage-antibiotic therapy regimens, suggesting that highly individualized treatment regimens should be used. We propose a set of experimental studies to address these research gaps. We end our review with suggestions on how to improve studies on phage-antibiotic combination therapy to advance this field.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"55-67"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of Phages That Bypass the Requirement for RNA-Mediated Antitermination. 绕过 RNA 介导的反凋亡要求的噬菌体的分离和特征描述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 Epub Date: 2023-06-19 DOI: 10.1089/phage.2023.0008

Rodney A King, Millicent Babbs, Kimberly Baugh, Courtney Hamilton

{"title":"Isolation and Characterization of Phages That Bypass the Requirement for RNA-Mediated Antitermination.","authors":"Rodney A King, Millicent Babbs, Kimberly Baugh, Courtney Hamilton","doi":"10.1089/phage.2023.0008","DOIUrl":"10.1089/phage.2023.0008","url":null,"abstract":"Introduction: The rpoCY75N mutation in the zinc-binding domain of the β' subunit of Escherichia coli RNA polymerase blocks the RNA-based mechanism of transcription antitermination utilized by bacteriophage HK022.Materials and methods: Mutant phages that overcome the block imposed by the rpoCY75N mutation are described. These phages, designated \"orc\" (overcomes rpoC), carry mutations that create new promoters. Promoter activity was assessed by cloning the respective regions from the wild-type and orc phages into a promoterless lacZ reporter vector.Results: Reporter assays showed that the sequence originating from orc phages had significant promoter activity when compared with the equivalent sequence cloned from the parental phage.Conclusions: The newly created promoters facilitate the expression of phage genes that are essential for growth on the rpoCY75N strain by bypassing transcription terminators. The small plaque phenotype of orc phages, when grown on the mutant host, suggests that suppression of the rpoCY75N mutation is incomplete.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"82-89"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9710350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Bacteriophages Members of the Ackermannviridae Family Specific for Klebsiella pneumoniae ST258. 对肺炎克雷伯氏菌 ST258 有特异性的阿克曼病毒科新噬菌体成员。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-06-01 Epub Date: 2023-06-19 DOI: 10.1089/phage.2022.0039

Estefanía Tisalema-Guanopatín, Fausto Cabezas-Mera, Karla Nolivos-Rodríguez, Isabel Fierro, Lourdes Pazmiño, Daniel Garzon-Chavez, Alexis Debut, Karla Vizuete, Jorge Aníbal Reyes

{"title":"New Bacteriophages Members of the Ackermannviridae Family Specific for Klebsiella pneumoniae ST258.","authors":"Estefanía Tisalema-Guanopatín, Fausto Cabezas-Mera, Karla Nolivos-Rodríguez, Isabel Fierro, Lourdes Pazmiño, Daniel Garzon-Chavez, Alexis Debut, Karla Vizuete, Jorge Aníbal Reyes","doi":"10.1089/phage.2022.0039","DOIUrl":"10.1089/phage.2022.0039","url":null,"abstract":"Background: Carbapenem-resistant Klebsiella pneumoniae, particularly isolates classified as sequence-type 258 (ST258), are multidrug-resistant strains that are strongly associated with poor-prognosis nosocomial infections, as current therapeutic options are limited and ineffective. In recent years, phage therapy has emerged as a promising treatment option for these scenarios.Methodology and results: We report the isolation and characterization of three new phages against Klebsiella pneumoniae ST258 strains recovered from Machángara river wastewater. These new members of the Ackermannviridae family showed stability over a wide temperature and pH range and burst sizes ranging from 6 to 44 plaque-forming units per bacteria. Their genomes were about 157 kilobases, with an average guanine-cytosine content of 46.4% and showed presence of several transfer RNAs, which also allowed us to predict in silico a lytic replicative cycle due to the presence of endolysins and lysozymes.Conclusion: Three lytic phages of Ackermannviridae family were recovered against Klebsiella pneumoniae ST258 strains from sewage; however, further characterization is needed for future consideration as therapeutic alternatives.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"99-107"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0