PHAGE (New Rochelle, N.Y.)最新文献_第10页

Genome Sequence and Characterization of Acinetobacter Phage DMU1. 不动杆菌噬菌体DMU1的基因组序列与特性研究。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0043

Bailey M Pehde, Devon Niewohner, Faithe E Keomanivong, Michael D Carruthers

{"title":"Genome Sequence and Characterization of Acinetobacter Phage DMU1.","authors":"Bailey M Pehde, Devon Niewohner, Faithe E Keomanivong, Michael D Carruthers","doi":"10.1089/phage.2020.0043","DOIUrl":"https://doi.org/10.1089/phage.2020.0043","url":null,"abstract":"Background: There has been a recent resurgence of research on the characterization of Acinetobacter phage for therapeutic use due to the morbidity and mortality associated with treatment failures in cases of multidrug-resistant Acinetobacter baumannii infections. Materials and Methods: A bacteriophage isolated from activated sludge that targets A. baumannii ATCC19606 was characterized by electron microscopy, genome sequencing, comparative genomics, and a host range analysis. Results: The morphology of Acinetobacter phage DMU1 resembles phages in Siphoviridae. Comparative genomic and phylogenetic analyses reveal that DMU1 is a siphophage and is most closely related to Acinetobacter phage SH-Ab 15497. Out of the strains tested, DMU1 was found to only infect A. baumannii strains ATCC19606 and ATCC17978. Conclusion: Acinetobacter phage DMU1 belongs to the Siphoviridae family and is most closely related to Acinetobacter phage SH-Ab 15497. Small-scale host-range analysis of DMU1 indicates a host range that is likely limited to specific A. baumannii strains.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"2 1","pages":"50-56"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041440/pdf/phage.2020.0043.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33478037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Call for Special Issue Papers: Phage Informatics and AI: Deadline for Manuscript Submission: August 31, 2021. 特刊论文征集:噬菌体信息学和人工智能:稿件提交截止日期:2021年8月31日。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2021.29011.cfp

Martha R J Clokie

引用次数: 0

Virus-Like Particle: Evolving Meanings in Different Disciplines. 类病毒粒子:在不同学科中的演变意义。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0026

Paul Hyman, Gareth Trubl, Stephen T Abedon

引用次数: 6

inPhocus: "Virus Amigos?" The Journey of the Development of Phage-Based Biocontrol in the Latin American Poultry and Aquaculture Industries. inPhocus:“病毒朋友?”以噬菌体为基础的生物防治在拉丁美洲家禽和水产养殖业的发展历程。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2021.29012.grp

Guillermo Rangel-Pineros, Thomas Sicheritz-Pontén, Martha R J Clokie

引用次数: 0

Tightening Bonds in Latin America Through Phage Discovery. 通过噬菌体发现拉紧拉丁美洲的纽带。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0028

Florencia Payaslian, Victoria Gradaschi, Liliana Rondón Salazar, Maria Eugenia Dieterle, Estefanía Urdániz, Matias Di Paola, Fabio Zon, Mariana Allievi, Carmen Sanchez Rivas, Raúl Ricardo Raya, Alejandro Reyes, Mariana Piuri

引用次数: 0

The Effect of Oxygen Availability on Bacteriophage Infection: A Review. 氧气对噬菌体感染的影响：综述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0041

Francesca E Hodges, Thomas Sicheritz-Pontén, Martha R J Clokie

{"title":"The Effect of Oxygen Availability on Bacteriophage Infection: A Review.","authors":"Francesca E Hodges, Thomas Sicheritz-Pontén, Martha R J Clokie","doi":"10.1089/phage.2020.0041","DOIUrl":"10.1089/phage.2020.0041","url":null,"abstract":"Bacteriophages offer a viable solution to addressing the global issue of bacterial resistance to antimicrobials. Although knowledge of bacteriophages has increased greatly since their discovery in 1915, a significant amount of what is currently known is based on studies conducted in model conditions and aerobic environments. There are a variety of environments in which bacteriophages could be applied to successfully replace or supplement antimicrobials in agriculture, food production, and human medicine where the amount of oxygen is limited. There is a need to use phages in oxygen-limited environments, but few studies have examined the impact oxygen-limited environments have on the ability of phages to kill their hosts. The work that has been done is, however, insightful and will likely stimulate this area that is growing in importance as our need to use phages grows. This review summarizes the studies to date that have reported the characteristics of phages in both oxygen-rich and oxygen-limited environments. We also discuss the importance of considering the ultimate environment a phage will be applied to when designing experiments to isolate and characterize phages for use in phage-based antimicrobial products.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"2 1","pages":"16-25"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041485/pdf/phage.2020.0041.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33478042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of vB_MsmS_Celfi: A New Mycobacterium tuberculosis Bacteriophage. vB_MsmS_Celfi：一种新的结核分枝杆菌噬菌体

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0030

Florencia Payaslian, Victoria Gradaschi, Liliana Rondón Salazar, Maria Eugenia Dieterle, Estefanía Urdániz, Matias Di Paola, José Peña Cárcamo, Fabio Zon, Ezequiel Sosa, Darío Fernandez Do Porto, Matthew Dunne, Pauline Goeller, Jochen Klumpp, Raúl Ricardo Raya, Alejandro Reyes, Mariana Piuri

{"title":"Isolation and Characterization of vB_MsmS_Celfi: A New Mycobacterium tuberculosis Bacteriophage.","authors":"Florencia Payaslian, Victoria Gradaschi, Liliana Rondón Salazar, Maria Eugenia Dieterle, Estefanía Urdániz, Matias Di Paola, José Peña Cárcamo, Fabio Zon, Ezequiel Sosa, Darío Fernandez Do Porto, Matthew Dunne, Pauline Goeller, Jochen Klumpp, Raúl Ricardo Raya, Alejandro Reyes, Mariana Piuri","doi":"10.1089/phage.2020.0030","DOIUrl":"10.1089/phage.2020.0030","url":null,"abstract":"Introduction: Because of the clinical relevance of Mycobacteria, and from a therapeutic perspective, there is an increasing interest to study phages that infect bacteria belonging to this genus. Materials and Methods: A phage was isolated from a soil sample, using Mycobacterium smegmatis as host. Its characterization included sequencing, annotation, and analysis of the genome, host range determination, and electron microscopy imaging. Results: Mycobacterium phage vB_MsmS_Celfi is a temperate phage able to infect Mycobacterium tuberculosis with high efficiency. From electron microscopy images, Celfi belongs to the Siphoviridae family. Genome analysis classified phage Celfi into cluster L, subcluster L2 of Actinobacteriophage clusters. Mycobacterium phage Celfi exhibits a Lysin B distant to those present in other members of the subcluster and other mycobacteriophages. Conclusions: The discovery of new phages that infect M. tuberculosis could contribute to the development of novel tools for detection systems and future treatment of the disease.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"2 1","pages":"43-49"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041486/pdf/phage.2020.0030.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33478041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Sequence and Characterization of Lactobacillus casei Phage, vB_LcaM_Lbab1 Isolated from Raw Milk. 从生乳中分离出的干酪乳杆菌噬菌体 vB_LcaM_Lbab1 的基因组序列和特征。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0029

Victoria Gradaschi, Florencia Payaslian, Maria Eugenia Dieterle, Liliana Rondón Salazar, Estefanía Urdániz, Matias Di Paola, José Peña Cárcamo, Fabio Zon, Mariana Allievi, Ezequiel Sosa, Darío Fernandez Do Porto, Matthew Dunne, Pauline Goeller, Jochen Klumpp, Raúl Ricardo Raya, Alejandro Reyes, Mariana Piuri

{"title":"Genome Sequence and Characterization of Lactobacillus casei Phage, vB_LcaM_Lbab1 Isolated from Raw Milk.","authors":"Victoria Gradaschi, Florencia Payaslian, Maria Eugenia Dieterle, Liliana Rondón Salazar, Estefanía Urdániz, Matias Di Paola, José Peña Cárcamo, Fabio Zon, Mariana Allievi, Ezequiel Sosa, Darío Fernandez Do Porto, Matthew Dunne, Pauline Goeller, Jochen Klumpp, Raúl Ricardo Raya, Alejandro Reyes, Mariana Piuri","doi":"10.1089/phage.2020.0029","DOIUrl":"10.1089/phage.2020.0029","url":null,"abstract":"Introduction: Only a few Lactobacillus casei phages have so far been characterized. As several L. casei strains are part of probiotic formulations, bacteriophage outbreaks targeting these strains can lead to critical losses within the dairy industry. Materials and Methods: A new L. casei phage was isolated from raw milk obtained from a milking yard from the province of Buenos Aires. The phage genome was sequenced, annotated, and analyzed. Morphology was determined by electron microscopy and the host range was established. Results: Lactobacillus phage vB_LcaM_Lbab1 is a member of the Herelleviridae family and features a host range including L. casei/Lactobacillus paracasei and Lactobacillus kefiri strains. We further analyzed the baseplate proteins in silico and found putative carbohydrate binding modules that are responsible for host recognition in other Lactobacillus phages. Conclusions: A new Lactobacillus phage was isolated and characterized. The focus was made on its host recognition mechanism, pointing toward the development of future strategies to avoid deleterious infections in the dairy industry.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"2 1","pages":"57-63"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041484/pdf/phage.2020.0029.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33478043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of Klebsiella Phages for Phage Therapy. 用于噬菌体疗法的克雷伯氏菌噬菌体的分离和特征描述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2020.0046

Eleanor M Townsend, Lucy Kelly, Lucy Gannon, George Muscatt, Rhys Dunstan, Slawomir Michniewski, Hari Sapkota, Saija J Kiljunen, Anna Kolsi, Mikael Skurnik, Trevor Lithgow, Andrew D Millard, Eleanor Jameson

{"title":"Isolation and Characterization of Klebsiella Phages for Phage Therapy.","authors":"Eleanor M Townsend, Lucy Kelly, Lucy Gannon, George Muscatt, Rhys Dunstan, Slawomir Michniewski, Hari Sapkota, Saija J Kiljunen, Anna Kolsi, Mikael Skurnik, Trevor Lithgow, Andrew D Millard, Eleanor Jameson","doi":"10.1089/phage.2020.0046","DOIUrl":"10.1089/phage.2020.0046","url":null,"abstract":"Introduction: Klebsiella is a clinically important pathogen causing a variety of antimicrobial resistant infections in both community and nosocomial settings, particularly pneumonia, urinary tract infection, and sepsis. Bacteriophage (phage) therapy is being considered a primary option for the treatment of drug-resistant infections of these types. Methods: We report the successful isolation and characterization of 30 novel, genetically diverse Klebsiella phages. Results: The isolated phages span six different phage families and nine genera, representing both lysogenic and lytic lifestyles. Individual Klebsiella phage isolates infected up to 11 of the 18 Klebsiella capsule types tested, and all 18 capsule-types were infected by at least one of the phages. Conclusions: Of the Klebsiella-infecting phages presented in this study, the lytic phages are most suitable for phage therapy, based on their broad host range, high virulence, short lysis period and given that they encode no known toxin or antimicrobial resistance genes. Phage isolates belonging to the Sugarlandvirus and Slopekvirus genera were deemed most suitable for phage therapy based on our characterization. Importantly, when applied alone, none of the characterized phages were able to suppress the growth of Klebsiella for more than 12 h, likely due to the inherent ease of Klebsiella to generate spontaneous phage-resistant mutants. This indicates that for successful phage therapy, a cocktail of multiple phages would be necessary to treat Klebsiella infections.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"2 1","pages":"26-42"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25556246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: Happy Reading! 社论:阅读愉快!

PHAGE (New Rochelle, N.Y.) Pub Date : 2021-03-01 Epub Date: 2021-03-17 DOI: 10.1089/phage.2021.29013.mco

Martha R J Clokie

引用次数: 0