PHAGE (New Rochelle, N.Y.)最新文献

Aerosolic Application of Phages Against S. infantis on Plates and Chicken Skin. 在平板和鸡皮上气溶胶应用噬菌体抗婴儿沙门氏菌

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2023.0037

Lisa Winkelmayer, Krista Rathammer, Susanne Richter, Theres Requat, Monika Matt, Davul Ljuhar, Philippe Jäger, Friedrich Kernmauner, Seyed Naemi, Michael Dieter Mansfeld, Georg G Duscher

引用次数: 0

Expanding the Phage Galaxy: Isolation and Characterization of Five Novel Streptomyces Siphoviruses Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo. 扩展噬菌体银河系：五种新型链霉菌虹彩病毒 Ankus、Byblos、DekoNeimoidia、Mandalore 和 Naboo 的分离与特征描述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.0012

Sebastian H Erdrich, Tom Luthe, Larissa Kever, Biel Badia Roigé, Borjana Arsova, Eva Davoudi, Julia Frunzke

{"title":"Expanding the Phage Galaxy: Isolation and Characterization of Five Novel Streptomyces Siphoviruses Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo.","authors":"Sebastian H Erdrich, Tom Luthe, Larissa Kever, Biel Badia Roigé, Borjana Arsova, Eva Davoudi, Julia Frunzke","doi":"10.1089/phage.2024.0012","DOIUrl":"10.1089/phage.2024.0012","url":null,"abstract":"Background: Key features of the actinobacterial genus Streptomyces are multicellular, filamentous growth, and production of a broad portfolio of bioactive molecules. These characteristics appear to play an important role in phage-host interactions and are modulated by phages during infection. To accelerate research of such interactions and the investigation of novel immune systems in multicellular bacteria, phage isolation, sequencing, and characterization are needed. This is a prerequisite for establishing systematic collections that appropriately cover phage diversity for comparative analyses.Material & methods: As part of a public outreach program within the priority program SPP 2330, involving local schools, we describe the isolation and characterization of five novel Streptomyces siphoviruses infecting S. griseus, S. venezuelae, and S. olivaceus.Results: All isolates are virulent members of two existing genera and, additionally, establish a new genus in the Stanwilliamsviridae family. In addition to an extensive set of tRNAs and proteins involved in phage replication, about 80% of phage genes encode hypothetical proteins, underlining the yet underexplored phage diversity and genomic dark matter still found in bacteriophages infecting actinobacteria.Conclusions: Taken together, phages Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo expand the phage diversity and contribute to ongoing research in the field of Streptomyces phage-host interactions.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 3","pages":"153-161"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives of Success. Cartoon by Ellie Jameson. 成功的视角。漫画：Ellie Jameson。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.57915.crt

引用次数: 0

Celebrating Progress and Overcoming Challenges in Phage Research. 庆祝噬菌体研究的进步和克服挑战。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.2908.edt

Martha Clokie, Thomas Sicheritz-Pontén

引用次数: 0

SalmoFree^® Phage Additive Proves Its Safety for Laying Hens. SalmoFree® 噬菌体添加剂证明了其对产蛋鸡的安全性。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.0010

Santiago Hernández Villamizar, Juliana Arias Bonilla, Álvaro Hugo Jaramillo, Ricardo Piñeros, Antonio Ripoll, Laura Fonseca, Karen Riveros, Martha J Vives, Paola Barato, Viviana Clavijo

{"title":"SalmoFree® Phage Additive Proves Its Safety for Laying Hens.","authors":"Santiago Hernández Villamizar, Juliana Arias Bonilla, Álvaro Hugo Jaramillo, Ricardo Piñeros, Antonio Ripoll, Laura Fonseca, Karen Riveros, Martha J Vives, Paola Barato, Viviana Clavijo","doi":"10.1089/phage.2024.0010","DOIUrl":"10.1089/phage.2024.0010","url":null,"abstract":"Background: The avian pathogen Salmonella Gallinarum causes avian typhosis in laying hens, leading to high mortality rates among adult birds, which poses a significant problem in the poultry industry. Various products, such as vaccines, antibiotics, probiotics, and disinfectants, are commonly used to prevent and control the disease on farms. An alternative to these products is the use of bacteriophages, which may effectively prevent the colonization of S. Gallinarum.Materials and methods: This study evaluated the safety of SalmoFree®, a bacteriophage cocktail, administered to 276 laying hens from the first week of age until the 28th week. The hens were divided into two groups: a control group (138 birds) and a treatment group (138 birds). Over the 28-week period, eight doses of SalmoFree® (∼1010 UFP per bird) were administered via drinking water in a controlled environment.Results: The results indicate that the consumption of SalmoFree® has no adverse effects on bird health or zootechnical parameters. Additionally, there is a trend toward improving weight homogeneity (up to 19%), feed conversion (up to 68%), and egg weight (up to 2.7%). The detection of phages by PCR in cloacal swabs suggests that they persist in birds for 2 to 8 weeks post-ingestion. Furthermore, phages were detected in organs and eggshells, indicating that they provide protection beyond the gut.Conclusion: The study demonstrates that SalmoFree® is safe for use in laying hens and may offer additional benefits, such as improved zootechnical parameters and extended protection against S. Gallinarum through the persistence of bacteriophages in the birds.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 3","pages":"143-152"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invade: Characterizing Phages through Their Actions and Genomes. Cartoon by Ellie Jameson. 入侵：通过噬菌体的作用和基因组来描述噬菌体的特征。漫画：Ellie Jameson。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2024.0019

引用次数: 0

Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3. 介绍分枝杆菌噬菌体 B3 亚簇成员 Casbah、Kronus 和 MmasiCarm。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2024.0004

Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia

{"title":"Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3.","authors":"Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia","doi":"10.1089/phage.2024.0004","DOIUrl":"10.1089/phage.2024.0004","url":null,"abstract":"Background: As part of a large science education effort, bacteriophages that lyse Mycobacterium smegmatis mc2155 continue to be discovered.Materials and methods: Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.Results: Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.Conclusions: Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and Gordonia DR phages.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 2","pages":"84-90"},"PeriodicalIF":0.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Biochemical Characterization of a New Phage-Encoded Muramidase, KTN6 Gp46. 一种新的噬菌体编码喃喃苷酶 KTN6 Gp46 的结构和生化特征。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2023.0040

Marta Sanz-Gaitero, Vincent De Maesschalck, Ankur Patel, Hannelore Longin, Vera Van Noort, Lorena Rodriguez-Rubio, Michael van Ryne, Katarzyna Danis-Wlodarczyk, Zuzanna Drulis-Kawa, Stephane Mesnage, Mark van Raaij, Rob Lavigne

{"title":"Structural and Biochemical Characterization of a New Phage-Encoded Muramidase, KTN6 Gp46.","authors":"Marta Sanz-Gaitero, Vincent De Maesschalck, Ankur Patel, Hannelore Longin, Vera Van Noort, Lorena Rodriguez-Rubio, Michael van Ryne, Katarzyna Danis-Wlodarczyk, Zuzanna Drulis-Kawa, Stephane Mesnage, Mark van Raaij, Rob Lavigne","doi":"10.1089/phage.2023.0040","DOIUrl":"10.1089/phage.2023.0040","url":null,"abstract":"Background: Endolysins are phage-encoded lytic enzymes that degrade bacterial peptidoglycan at the end of phage lytic cycles to release new phage particles. These enzymes are being explored as an alternative to small-molecule antibiotics.Methods: The crystal structure of KTN6 Gp46 was determined and compared with a ColabFold model. Cleavage specificity was examined using a peptidoglycan digest and reversed-phase high-performance liquid chromatography coupled to mass spectrometry (HPLC/MS).Results: The structure of KTN6 Gp46 could be determined at 1.4 Å resolution, and key differences in loops of the putative peptidoglycan binding domain were identified in comparison with its closest known homologue, the endolysin of phage SPN1S. Reversed-phase HPLC/MS analysis of the reaction products following peptidoglycan digestion confirmed the muramidase activity of Gp46, consistent with structural predictions.Conclusion: These insights into the structure and function of endolysins further expand the toolbox for endolysin engineering and explore their potential in enzyme-based antibacterial design strategies.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 2","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial June 2024. 社论 2024 年 6 月。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2024.0020

Martha Rebecca Jane Clokie, Thomas Sicheritz-Pontén

引用次数: 0

Phage Therapy in the Management of Urinary Tract Infections: A Comprehensive Systematic Review. 噬菌体治疗在尿路感染治疗中的应用：一项全面的系统综述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.0024

Amany M Al-Anany, Payton B Hooey, Jonathan D Cook, Lori L Burrows, Julia Martyniuk, Alexander P Hynes, Greg J German

{"title":"Phage Therapy in the Management of Urinary Tract Infections: A Comprehensive Systematic Review.","authors":"Amany M Al-Anany, Payton B Hooey, Jonathan D Cook, Lori L Burrows, Julia Martyniuk, Alexander P Hynes, Greg J German","doi":"10.1089/phage.2023.0024","DOIUrl":"https://doi.org/10.1089/phage.2023.0024","url":null,"abstract":"Urinary tract infections (UTIs) are a problem worldwide, affecting almost half a billion people each year. Increasing antibiotic resistance and limited therapeutic options have led to the exploration of alternative therapies for UTIs, including bacteriophage (phage) therapy. This systematic review aims at evaluating the efficacy of phage therapy in treating UTIs. We employed a comprehensive search strategy for any language, any animal, and any publication date. A total of 55 in vivo and clinical studies were included. Of the studies, 22% were published in a non-English language, 32.7% were before the year 1996, and the rest were after 2005. The results of this review suggest that phage therapy for UTIs can be effective; more than 72% of the included articles reported microbiological and clinical improvements. On the other hand, only 5 randomized controlled trials have been completed, and case reports and case series information were frequently incomplete for analysis. Overall, this comprehensive systematic review identifies preliminary evidence supporting the potential of phage therapy as a safe and viable option for the treatment of UTIs.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"112-127"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1