PHAGE (New Rochelle, N.Y.)最新文献

Isolation and Characterization of Three Lytic Bacteriophages to Overcome Multidrug-, Extensive Drug-, and Pandrug-Resistant Pseudomonas aeruginosa. 三种抗多药、广泛耐药和泛耐药铜绿假单胞菌的噬菌体的分离和鉴定。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2021.0018

Marwan Mahmood Saleh, Majeed Arsheed Sabbah, Zahraa Kamel Zedan

{"title":"Isolation and Characterization of Three Lytic Bacteriophages to Overcome Multidrug-, Extensive Drug-, and Pandrug-Resistant Pseudomonas aeruginosa.","authors":"Marwan Mahmood Saleh, Majeed Arsheed Sabbah, Zahraa Kamel Zedan","doi":"10.1089/phage.2021.0018","DOIUrl":"10.1089/phage.2021.0018","url":null,"abstract":"Background: The worrisome spread of multidrug-resistant (MDR) pathogens necessitates research on nonantibiotic therapeutics. Among these therapeutics, phage treatment uses bacteriophages (phages) as alternative antimicrobial agents.Objectives: This project evaluates the lytic efficiency of phage cocktails in vitro versus MDR, extensive drug-resistant (XDR), and pandrug-resistant (PDR) P. aeruginosa isolates.Methods: We utilized host range and genetic information to generate a three-phage cocktail capable of killing multiple clinical strains of P. aeruginosa and examined the effectiveness of the cocktail in this study. The isolates (114) had variable resistance to 13 antibiotics. A phage-enrichment approach was used to purify the bacteriophage cocktail; a phage lysate with a high titer (5 × 109 PFU/mL) was prepared and tested against 114 P. aeruginosa isolates.Findings: The results showed that a cocktail of three phages (MMS1, MMS2, and MMS3) could lyse P. aeruginosa in both planktonic liquid and dish cultures. The MMS cocktail phages were shown to be viable between 4 and 50°C at pH 4-9. A one-step growth curve showed that the MMS phages had a latent period of 15 min and a burst period of approximately 18 min based on the size of approximately 265 offspring phages per host cell. The MMS3 phage was sequenced and shown to lack genes associated with bacterial pathogenicity or antibiotic resistance.Conclusions: Notably, XDR and PDR isolates were sensitive to the phage cocktail, a prospective substitute for antibiotics that does not contribute to the growth of antibiotic resistance, suggesting that the phage cocktail might be useful for generating personalized phage therapeutics.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 4","pages":"230-240"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Correction to: Addressing the Research and Development Gaps in Modern Phage Therapy, by Paul E. Turner et al., PHAGE: Ther Appl Res 2024;5(1):30-39; doi: 10.1089/phage.2023.0045. 更正：解决现代噬菌体治疗的研究和发展差距，由Paul E. Turner等人，噬菌体：Ther apple Res 2024；5(1):30-39；doi: 10.1089 / phage.2023.0045。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2023.0045.correx

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A New Insight into Phage Combination Therapeutic Approaches Against Drug-Resistant Mixed Bacterial Infections. 噬菌体联合治疗耐药混合细菌感染的新认识

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2024.0011

Mohammadreza Rahimian, Davoud Jafari-Gharabaghlou, Elham Mohammadi, Nosratollah Zarghami

{"title":"A New Insight into Phage Combination Therapeutic Approaches Against Drug-Resistant Mixed Bacterial Infections.","authors":"Mohammadreza Rahimian, Davoud Jafari-Gharabaghlou, Elham Mohammadi, Nosratollah Zarghami","doi":"10.1089/phage.2024.0011","DOIUrl":"10.1089/phage.2024.0011","url":null,"abstract":"Rising antibiotic resistance among bacterial pathogens has become a substantial health issue for human civilization. The emergence of these pathogens in high-risk diseases such as cystic fibrosis (CF) has led to financial and nonfinancial losses, necessitating alternative therapies. This study presents an overview of such approaches, including phage therapy, antimicrobial peptides (AMPs), nanotechnology, monoclonal antibodies (mAbs), microbial therapies (probiotic therapy), clustered regularly interspaced short palindromic repeats technology (CRISPR), and aptamers focusing on their mechanisms of action and exploring the impact of combining phage and phage derivatives with the mentioned approaches. Although alternative approaches and their combinations with phages show promise, the phage-antibiotic combination has been the subject of most studies, and It has been proven to be highly effective in combating antibiotic-resistant infections. Other combinations also appear promising, but further studies are needed to determine their effectiveness. This emphasizes the need for more thorough research into different phage combination treatments beyond the well-established phage-antibiotic strategy.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 4","pages":"203-222"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Editorial. 社论。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2024.0065

Martha Clokie, Thomas Sicheritz-Pontén

引用次数: 0

Beyond Antibiotics: Exploring the Potential of Bacteriophages and Phage Therapy. 超越抗生素：探索噬菌体和噬菌体治疗的潜力。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2024.0005

Sudhangshu Kumar Biswas, Md Mehedi Hasan Sumon, Sabbir Ahmed, Rumana Akter Ruma, Anzana Parvin, Dipak Kumar Paul, Apurba Kumar Roy, Swee-Seong Tang

{"title":"Beyond Antibiotics: Exploring the Potential of Bacteriophages and Phage Therapy.","authors":"Sudhangshu Kumar Biswas, Md Mehedi Hasan Sumon, Sabbir Ahmed, Rumana Akter Ruma, Anzana Parvin, Dipak Kumar Paul, Apurba Kumar Roy, Swee-Seong Tang","doi":"10.1089/phage.2024.0005","DOIUrl":"10.1089/phage.2024.0005","url":null,"abstract":"The escalating global threat of antibiotic resistance has prompted a critical need for innovative approaches to bacterial infection treatment. In terms of management, bacterial-associated disorders have reached a critical point in the world due to the advent of drug-resistant types of bacteria. Nonetheless, continued bacteriophage research presents a promising frontier in the battle against bacterial infections. In this study, we explored the effectiveness of bacteriophage therapy against antibiotic-resistant bacteria. According to our viewpoint, phage therapy could replace antibiotics in terms of safety, efficacy, and specificity. Furthermore, phage therapy offers versatility in administering single phage, phage products, or modified phage against various resistant bacteria. Moreover, Phage cocktails, with their synergistic combinations, showcase a holistic approach, mitigating the risk of resistance and expanding treatment possibilities. Similarly, the synergy between phages and antibiotics holds promise for overcoming antibiotic resistance and forging a path toward more effective and sustainable antimicrobial strategies. The review provides insights into the transformative impact of bacteriophage therapy on current bacterial infection treatment paradigms, its application with methodological challenges and limitations, as well as insights to scientists and policymakers on the best areas to study phages in order to combat antimicrobial resistance.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 4","pages":"186-202"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Bacteriophage Dosing and Its Effect on Bacterial Growth Suppression in a Staphylococcus epidermidis Model: An In Vitro Study. 噬菌体剂量及其对表皮葡萄球菌模型细菌生长抑制作用的体外研究

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2024.0001

Jason Young, Mohammad Javad Shariyate, Ahmad Hedayatzadeh Razavi, Ara Nazarian, Edward K Rodriguez

{"title":"Bacteriophage Dosing and Its Effect on Bacterial Growth Suppression in a Staphylococcus epidermidis Model: An In Vitro Study.","authors":"Jason Young, Mohammad Javad Shariyate, Ahmad Hedayatzadeh Razavi, Ara Nazarian, Edward K Rodriguez","doi":"10.1089/phage.2024.0001","DOIUrl":"10.1089/phage.2024.0001","url":null,"abstract":"Background: Phages are an emerging therapy in the treatment of prosthetic joint infections, though many challenges remain, including an incomplete understanding of optimal phage dosing.Materials and methods: We performed an in vitro assessment of how phage dosing as measured by multiplicity of infection (MOI) impacts bacterial growth in planktonic and biofilm conditions using a Staphylococcus epidermidis model. Staphylococcus epidermidis ATCC 35984 was combined in planktonic and biofilm forms with phage vB_SepM_Alex at varying concentrations, and growth was monitored via spectrophotometry.Results: Planktonic bacterial growth was significantly higher when MOI ≤ 0.01 compared with MOI ≥ 10 (p < 0.05). Biofilms with phage dosing at ≤ 104 plaque-forming units (PFU)/mL had significantly greater spectrophotometer readings than those dosed at 1010 PFU/mL (p < 0.05).Conclusions: Our findings suggest lower, not higher, phage dosing is associated with greater bacterial persistence. Our study helps inform the dosing and delivery of this alternative form of antibiosis.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 4","pages":"223-229"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization of Salmonella Phages and Absence of Salmonella Strains from Three Different Study Areas of Cattle Livestock. 沙门菌噬菌体的鉴定与特征及三个不同研究区沙门菌的缺失。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI: 10.1089/phage.2023.0048

Dácil Rivera, Kasim Allel, Carla Salazar, Christopher Hamilton-West, Fernando Dueñas, Luis Pablo Hervé, Andrea I Moreno-Switt

{"title":"Identification and Characterization of Salmonella Phages and Absence of Salmonella Strains from Three Different Study Areas of Cattle Livestock.","authors":"Dácil Rivera, Kasim Allel, Carla Salazar, Christopher Hamilton-West, Fernando Dueñas, Luis Pablo Hervé, Andrea I Moreno-Switt","doi":"10.1089/phage.2023.0048","DOIUrl":"10.1089/phage.2023.0048","url":null,"abstract":"Background: Salmonella represents a significant risk to both public and animal health. Although Salmonella strains have not been isolated from cattle livestock, Salmonella phages have been successfully identified.Materials and methods: Our study was conducted through (i) investigating the presence of Salmonella strains and Salmonella phages in three study areas, (ii) phenotypic screening by lytic profile (LP); (iii) selecting nine phages for sequencing; and (iv) genomic comparison to evaluate their relative diversity.Results: A total of 307 samples were analyzed, resulting in a total of 162 virus-like particles (VLPs) analyzed. The LP was performed to identify Salmonella strains susceptible to phage infection, including the most frequent serovars: Dublin, Enteritidis, and Javiana. From the VLPs, nine phages were selected for genomic comparison. These phages represent three morphotypes: siphoviruses, myoviruses, and podoviruses, originating from different geographic and productive sites.Conclusions: This study enhances the understanding of the presence and diversity of Salmonella phages in cattle livestock, even in the absence of Salmonella strains.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 4","pages":"241-252"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aerosolic Application of Phages Against S. infantis on Plates and Chicken Skin. 在平板和鸡皮上气溶胶应用噬菌体抗婴儿沙门氏菌

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2023.0037

Lisa Winkelmayer, Krista Rathammer, Susanne Richter, Theres Requat, Monika Matt, Davul Ljuhar, Philippe Jäger, Friedrich Kernmauner, Seyed Naemi, Michael Dieter Mansfeld, Georg G Duscher

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Expanding the Phage Galaxy: Isolation and Characterization of Five Novel Streptomyces Siphoviruses Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo. 扩展噬菌体银河系：五种新型链霉菌虹彩病毒 Ankus、Byblos、DekoNeimoidia、Mandalore 和 Naboo 的分离与特征描述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.0012

Sebastian H Erdrich, Tom Luthe, Larissa Kever, Biel Badia Roigé, Borjana Arsova, Eva Davoudi, Julia Frunzke

{"title":"Expanding the Phage Galaxy: Isolation and Characterization of Five Novel Streptomyces Siphoviruses Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo.","authors":"Sebastian H Erdrich, Tom Luthe, Larissa Kever, Biel Badia Roigé, Borjana Arsova, Eva Davoudi, Julia Frunzke","doi":"10.1089/phage.2024.0012","DOIUrl":"10.1089/phage.2024.0012","url":null,"abstract":"Background: Key features of the actinobacterial genus Streptomyces are multicellular, filamentous growth, and production of a broad portfolio of bioactive molecules. These characteristics appear to play an important role in phage-host interactions and are modulated by phages during infection. To accelerate research of such interactions and the investigation of novel immune systems in multicellular bacteria, phage isolation, sequencing, and characterization are needed. This is a prerequisite for establishing systematic collections that appropriately cover phage diversity for comparative analyses.Material & methods: As part of a public outreach program within the priority program SPP 2330, involving local schools, we describe the isolation and characterization of five novel Streptomyces siphoviruses infecting S. griseus, S. venezuelae, and S. olivaceus.Results: All isolates are virulent members of two existing genera and, additionally, establish a new genus in the Stanwilliamsviridae family. In addition to an extensive set of tRNAs and proteins involved in phage replication, about 80% of phage genes encode hypothetical proteins, underlining the yet underexplored phage diversity and genomic dark matter still found in bacteriophages infecting actinobacteria.Conclusions: Taken together, phages Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo expand the phage diversity and contribute to ongoing research in the field of Streptomyces phage-host interactions.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 3","pages":"153-161"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Perspectives of Success. Cartoon by Ellie Jameson. 成功的视角。漫画：Ellie Jameson。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.57915.crt

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