PHAGE (New Rochelle, N.Y.)最新文献

Characterization of Enterococcus faecium-Targeting Minhovirus Bacteriophages. 以粪肠球菌为靶点的微小病毒噬菌体的鉴定。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2026-06-01 Epub Date: 2026-01-13 DOI: 10.1177/26416549251413133

Eric Evans, Shinjiro Ojima, Wakana Yamashita, Kotaro Kiga, Daria Van Tyne

{"title":"Characterization of Enterococcus faecium-Targeting Minhovirus Bacteriophages.","authors":"Eric Evans, Shinjiro Ojima, Wakana Yamashita, Kotaro Kiga, Daria Van Tyne","doi":"10.1177/26416549251413133","DOIUrl":"10.1177/26416549251413133","url":null,"abstract":"Background: Enterococcus faecium are gram-positive, multidrug-resistant bacteria that are endemic in healthcare settings. Bacteriophage therapy could be an adjunctive treatment for E. faecium infections.Materials and methods: We isolated four new Minhovirus phages using E. faecium strain ATCC 19434 as a host.Results: Phage genomes were each ~19 kb in length, and were highly related to each other and to other previously isolated Minhovirus phages. Host range testing suggested a narrow spectrum of activity. In vitro-evolved phage-resistant mutants acquired mutations in the enterococcal polysaccharide antigen (epa) and capsular polysaccharide (cps) loci. Additional testing found that some phages were able to infect E. faecium clinical isolates with similar epa loci, further implicating epa as a putative receptor for Minhovirus phage infection.Conclusions: Overall, this study increases our understanding of phages that target E. faecium and increases the number of characterized phages that are active against this important pathogen.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"7 2","pages":"147-155"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13460231/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148715066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jumping Over Hurdles. 跳过跨栏。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2026-04-06 eCollection Date: 2026-03-01 DOI: 10.1177/26416549261432842

Ellie Jameson

引用次数: 0

Integrating Past and Present PHAGE Research. 整合过去和现在的噬菌体研究。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2026-03-09 eCollection Date: 2026-03-01 DOI: 10.1177/26416549261430532

Martha Clokie, Thomas Sicheritz-Pontén

引用次数: 0

Discovery and characterization of a novel Pseudomonas phage of a new genus. 新属假单胞菌噬菌体的发现和鉴定。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2026-02-08 DOI: 10.1177/26416549251406410

Paul Nicholls, Justin R Clark, Anthony Maresso

{"title":"Discovery and characterization of a novel Pseudomonas phage of a new genus.","authors":"Paul Nicholls, Justin R Clark, Anthony Maresso","doi":"10.1177/26416549251406410","DOIUrl":"10.1177/26416549251406410","url":null,"abstract":"Background: Bacteriophages exhibit great diversity and are classically divided into lytic and temperate lifestyles. Commonly, bioinformatics is used to predict the temperate lifestyle. As the nosocomial pathogen Pseudomonas aeruginosa is common in freshwater, this biome represents an ideal location for the discovery of Pseudomonas phages.Materials and methods: We characterized a genetically distinct species of Pseudomonas phage using next-generation sequencing and conventional microbiological methods. We characterized its lifestyle by purifying infected colonies and identifying persistent phage production.Results: Genetics revealed a phage of a novel genus. Characterization showed a narrow host range and bioinformatics suggested a lytic lifestyle. Experimental data showed lysogeny and analysis was consistent with an episomal lifestyle instead of the classic model of integration into the host genome.Conclusions: We identify and characterize a new Pseudomonas phage, show potential limitations of bioinformatic predictions of lifestyle, and further contribute to the diversity of the phage tree of life.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12927667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147286670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KP-C01: A Novel Phage with Tail-Associated Depolymerase Activity Against KL102 Carbapenem-Resistant Klebsiella pneumoniae. 具有抗KL102耐碳青霉烯肺炎克雷伯菌尾部相关解聚合酶活性的新型噬菌体KP-C01

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2026-01-14 eCollection Date: 2026-03-01 DOI: 10.1177/26416549251406409

Lan Yang, Jie Li, Jia Dai, Jianfang Liu, Demeng Tan, Nannan Wu, Tongyu Zhu, Mengjun Cheng

{"title":"KP-C01: A Novel Phage with Tail-Associated Depolymerase Activity Against KL102 Carbapenem-Resistant Klebsiella pneumoniae.","authors":"Lan Yang, Jie Li, Jia Dai, Jianfang Liu, Demeng Tan, Nannan Wu, Tongyu Zhu, Mengjun Cheng","doi":"10.1177/26416549251406409","DOIUrl":"10.1177/26416549251406409","url":null,"abstract":"Background: The emergence and spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) represent a major threat to global public health, necessitating effective therapeutic strategies to prevent infection.Materials and methods: A novel bacteriophage, φKP-C01, was isolated and characterized using ST307KL102 CRKP as the host.Results: One-step growth curve analysis and complete genome sequencing demonstrated φKP-C01 to be a highly efficient lytic phage. The absence of genes encoding virulence factors, drug resistance determinants, or lysogeny-related functions indicated its potential safety for therapeutic applications. Bioinformatic analysis identified orf5 as encoding Depo-C01, a putative tail fiber protein with predicted depolymerase activity. To validate this function, orf5 was cloned, and recombinant Depo-C01 was expressed and purified. Depolymerase activity specifically targeting the KL102 capsular polysaccharide of the CRKP host was confirmed via spot assay and transmission electron microscopy.Conclusion: Lytic phage φKP-C01 and its depolymerase Depo-C01 show strong potential for phage-based or enzymatic strategies targeting CRKP infections.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"7 1","pages":"35-42"},"PeriodicalIF":1.8,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jumping Over Hurdles: Challenges and Solutions in Phage Therapy. 跨越障碍：噬菌体治疗的挑战和解决方案。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2025-12-22 eCollection Date: 2026-03-01 DOI: 10.1177/26416549251409584

Mohammed A Imam

{"title":"Jumping Over Hurdles: Challenges and Solutions in Phage Therapy.","authors":"Mohammed A Imam","doi":"10.1177/26416549251409584","DOIUrl":"10.1177/26416549251409584","url":null,"abstract":"Bacteriophage therapy, which employs bacteriophages to eliminate bacteria, is gaining attention as a promising alternative to antibiotics amid mounting antimicrobial resistance. It has shown potential in treating chronic, multidrug-resistant infections, especially in vulnerable populations such as transplant patients. Moreover, advances in genomics, synthetic biology, and CRISPR-associated protein 9 have enabled the engineering of phages with broader host ranges and improved safety, while tools such as phage libraries and innovative delivery systems have enhanced its therapeutic effectiveness. Personalized phage therapy, guided by individual microbiota profiles, has demonstrated encouraging clinical outcomes as well. However, challenges remain, including regulatory barriers, safety concerns, and the emergence of phage-resistant bacteria. In response, continued research is essential to optimize treatment protocols and validate their clinical efficacy in standardized trials. Future priorities should include refining phage engineering, building robust regulatory frameworks, and establishing clear clinical guidelines. With coordinated global efforts, phage therapy could become a powerful, widely accessible tool in the ongoing battle against antibiotic-resistant infections.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"7 1","pages":"51-61"},"PeriodicalIF":1.8,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriophages for Controlling Multidrug-Resistant Klebsiella pneumoniae on Common Clinical Surface Materials. 临床常用表面材料控制多重耐药肺炎克雷伯菌的噬菌体研究。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2025-12-19 eCollection Date: 2026-03-01 DOI: 10.1177/26416549251406406

Andrea Katherine Álvarez Osorio, Juliana Valentina Arias Bonilla, Juliana Vanessa Rincón López, Eliana Maldonado Lara, Lina María Prieto Garzón, Luis Alberto Chica Cárdenas, Adriana Marcela Celis Ramírez, Martha Josefina Vives Flórez

{"title":"Bacteriophages for Controlling Multidrug-Resistant Klebsiella pneumoniae on Common Clinical Surface Materials.","authors":"Andrea Katherine Álvarez Osorio, Juliana Valentina Arias Bonilla, Juliana Vanessa Rincón López, Eliana Maldonado Lara, Lina María Prieto Garzón, Luis Alberto Chica Cárdenas, Adriana Marcela Celis Ramírez, Martha Josefina Vives Flórez","doi":"10.1177/26416549251406406","DOIUrl":"10.1177/26416549251406406","url":null,"abstract":"Introduction: The prevalence of Klebsiella pneumoniae on surfaces, its increased antibiotic resistance, and strain-specific disinfectant resistance underscore the need for alternative decontamination methods.Materials and methods: This study investigated the potential capacity of three newly isolated bacteriophages to control multidrug-resistant K. pneumoniae on common clinical surface materials (steel, fabric, PVC, and polystyrene). To the best of our knowledge, no survival studies of K. pneumoniae on these materials have yet been conducted.Results: K. pneumoniae remained viable on surfaces for up to 24 h. After 24 h, reductions in bacterial counts were lower at 24°C (0.49-1.59 log colony-forming units [CFU]) than those observed at 37°C (1.69-3.88 log CFU). These results are noteworthy as temperatures in intensive care units (ICUs) and surgical wards are typically 20-26°C and 20-23°C, respectively. Applying the phages at multiplicity of infection (MOI) 1000 achieved reductions of up to 4.99 log CFU at hour six across the surfaces at 37°C, while in the control tests (without phages) the counts decreased by <1 log CFU. Comparison with a commercial disinfectant revealed a faster initial reduction in bacterial counts; however, regrowth occurred over time. In contrast, the phages steadily decreased the clinical strain's bacterial counts over 6 h at 24°C.Conclusions: These findings suggest that bacteriophages can be used as effective disinfectants and as valuable tools for outbreak control in health care settings.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"7 1","pages":"21-34"},"PeriodicalIF":1.8,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129561/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Forgotten Phage Thinker: Bacteriophage Research in USSR in 1930s and the Contribution by Professor M.I. Lurie. 被遗忘的噬菌体思考者：20世纪30年代苏联的噬菌体研究及M.I. Lurie教授的贡献。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2025-12-19 eCollection Date: 2026-03-01 DOI: 10.1177/26416549251408686

Andrey V Letarov

引用次数: 0

Simple Catheter Biofilm Flow Model: Klebsiella Phages Disrupt E. coli Biofilms on Urinary Catheters Under Static and Dynamic Flow Conditions. 简单导尿管生物膜流动模型：克雷伯菌噬菌体在静态和动态流动条件下破坏导尿管上的大肠杆菌生物膜。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2025-12-03 eCollection Date: 2025-12-01 DOI: 10.1177/26416549251366989

Hoda Bseikri, Slawomir Michniewski, Eduardo Goicoechea Serrano, Eleanor Jameson

{"title":"Simple Catheter Biofilm Flow Model: Klebsiella Phages Disrupt E. coli Biofilms on Urinary Catheters Under Static and Dynamic Flow Conditions.","authors":"Hoda Bseikri, Slawomir Michniewski, Eduardo Goicoechea Serrano, Eleanor Jameson","doi":"10.1177/26416549251366989","DOIUrl":"https://doi.org/10.1177/26416549251366989","url":null,"abstract":"Background: Biofilms pose a significant challenge in medical settings, leading to persistent infections. Phage therapy shows promise in biofilm eradication, but its effectiveness under dynamic flow conditions remains unclear.Methods: We used two novel phages isolated on Klebsiella, Llofrudd and Samara, and characterized their genomes, host range, virulence, and impact on biofilms. In this study, we built a simple catheterized bladder model with flow to investigate the impact of phage treatment on biofilm viability in a flow-based catheter model.Results: Our analyses demonstrate that phages Llofrudd and Samara are the same species and infect a limited number of strains (3/221), but crucially across three species: Klebsiella aerogenes, Klebsiella pneumoniae, and E. coli.Conclusions: Phage treatment significantly reduced E. coli biofilm viability in catheters both in static conditions and under flow and liberated bacteria from the biofilms, highlighting the potential of phage therapy as an intervention strategy for catheter-associated urinary tract infections (CAUTI).","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 4","pages":"242-249"},"PeriodicalIF":1.8,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12929005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147291917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Catheters to Capsids-New Insights from the Phage Frontier. 从导管到衣壳——噬菌体前沿的新见解。

IF 1.8

PHAGE (New Rochelle, N.Y.) Pub Date : 2025-12-03 eCollection Date: 2025-12-01 DOI: 10.1177/26416549251401292

Martha Clokie, Thomas Sicheritz-Pontén

引用次数: 0