PHAGE (New Rochelle, N.Y.)最新文献

{"title":"Acknowledgment of Reviewers 2024.","authors":"","doi":"10.1089/phage.2024.25632.revack","DOIUrl":"https://doi.org/10.1089/phage.2024.25632.revack","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"48"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cartoon by Dr. Ellie Jameson, Bangor University.","authors":"","doi":"10.1089/phage.2025.7150","DOIUrl":"https://doi.org/10.1089/phage.2025.7150","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144060198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New <i>Morganella morganii</i> Phage vB_Mm5 with Tolerance to Cu²⁺ Ions.","authors":"Rusudan Goliadze, Luka Kamashidze, Natia Karumidze, Sophio Rigvava, Olia Rcheulishvili, Aleksander Rcheulishvili, Marine Goderdzishvili, Ia Kusradze","doi":"10.1089/phage.2024.0036","DOIUrl":"https://doi.org/10.1089/phage.2024.0036","url":null,"abstract":"<p><strong>Background: </strong>Research on phages targeting <i>Morganella morganii</i> is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling <i>M. morganii</i> spread in the environment.</p><p><strong>Materials and methods: </strong>This study investigates the biology and genetics of the novel <i>M. morganii</i>-infecting myophage <i>vB_Mm5</i> and evaluates its stability under Cu²⁺ exposure.</p><p><strong>Results: </strong>Phage <i>vB_Mm5</i> has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu²⁺. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.</p><p><strong>Conclusions: </strong><i>vB_Mm5</i> is highly distinct from other sequenced <i>M. morganii</i> phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against <i>M. morganii</i> infections.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"41-47"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phage-Encoded Antimicrobial Peptide gp28 Demonstrates LL-37-Like Antimicrobial Activity Against Multidrug-Resistant <i>Pseudomonas aeruginosa</i>.","authors":"Rachael C Wilkinson, Nerissa E Thomas, Amita Bhatti, Matthew R Burton, Naomi Joyce, Rowena E Jenkins","doi":"10.1089/phage.2024.0009","DOIUrl":"https://doi.org/10.1089/phage.2024.0009","url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) is a gram-negative bacterial pathogen commonly associated with nosocomial infections. Treatment of <i>P. aeruginosa</i> infections is notoriously difficult due to biofilm formation and antibiotic resistance. Antimicrobial peptides (AMPs) are thought to be promising new antimicrobials. Gp28, a phage-derived AMP, is a novel class of characterized phage AMPs with activity against <i>Escherichia coli</i> in a manner similar to the human peptide LL-37. LL-37 exhibits strong antimicrobial activity against <i>P. aeruginosa</i> as well as biofilm disruption and synergy with certain antibiotics posing the question whether gp28 could act similarly.</p><p><strong>Methods: </strong>Antibacterial activity of gp28 against <i>P. aeruginosa</i> was established using growth inhibition assays, with minimum inhibitory concentration calculated. Biofilm disruption was assessed using crystal violet staining and scanning electron microscopy. Combined treatment of gp28 with tobramycin against <i>P. aeruginosa</i> was measured using a modified time-kill assay at sublethal concentrations.</p><p><strong>Results: </strong>Gp28 inhibits <i>P. aeruginosa</i> planktonic growth, with a minimum inhibitory concentration of 109 μg mL^-1 and disrupts established biofilms. We demonstrate that gp28 increases the susceptibility of <i>P. aeruginosa</i> to tobramycin.</p><p><strong>Conclusions: </strong>Gp28 demonstrates potential for development as a putative therapeutic agent against a clinically resistant <i>P. aeruginosa</i> strain either alone or in combination with the frontline antibiotic tobramycin.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"12-19"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semantics Count in the Description of the Interactions Between Bacteria and Bacteriophage.","authors":"Brandon A Berryhill, Bruce R Levin","doi":"10.1089/phage.2024.0063","DOIUrl":"https://doi.org/10.1089/phage.2024.0063","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"3-4"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"taxMyPhage: Automated Taxonomy of dsDNA Phage Genomes at the Genus and Species Level.","authors":"Andrew Millard, Rémi Denise, Maria Lestido, Moi Taiga Thomas, Deven Webster, Dann Turner, Thomas Sicheritz-Pontén","doi":"10.1089/phage.2024.0050","DOIUrl":"https://doi.org/10.1089/phage.2024.0050","url":null,"abstract":"<p><strong>Background: </strong>Bacteriophages are classified into genera and species based on genomic similarity, a process regulated by the International Committee on the Taxonomy of Viruses. With the rapid increase in phage genomic data there is a growing need for automated classification systems that can handle large-scale genome analyses and place phages into new or existing genera and species.</p><p><strong>Materials and methods: </strong>We developed <i>taxMyPhage</i>, a tool system for the rapid automated classification of dsDNA bacteriophage genomes. The system integrates a MASH database, built from ICTV-classified phage genomes to identify closely related phages, followed by BLASTn to calculate intergenomic similarity, conforming to ICTV guidelines for genus and species classification. taxMyPhage is available as a git repository at https://github.com/amillard/tax_myPHAGE, a conda package, a pip-installable tool, and a web service at https://phagecompass.ku.dk.</p><p><strong>Results: </strong><i>taxMyPhage</i> enables rapid classification of bacteriophages to the genus and species level. Benchmarking on 705 genomes pending ICTV classification showed a 96.7% accuracy at the genus level and 97.9% accuracy at the species level. The system also detected inconsistencies in current ICTV classifications, identifying cases where genera did not adhere to ICTV's 70% average nucleotide identity (ANI) threshold for genus classification or 95% ANI for species. The command line version classified 705 genomes within 48 h, demonstrating its scalability for large datasets.</p><p><strong>Conclusions: </strong><i>taxMyPhage</i> significantly enhances the speed and accuracy of bacteriophage genome classification at the genus and species levels, making it compatible with current sequencing outputs. The tool facilitates the integration of bacteriophage classification into standard workflows, thereby accelerating research and ensuring consistent taxonomy.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"5-11"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144054876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradation of Preformed Gram-Positive and Gram-Negative Bacterial Biofilms Using Disintegrated and Intact Phages.","authors":"Sahd Ali, Abdulkerim Karaynir, Hanife Salih Doğan, Ramesh Nachimuthu, Kingsley Badu, Bülent Bozdoğan","doi":"10.1089/phage.2024.0029","DOIUrl":"https://doi.org/10.1089/phage.2024.0029","url":null,"abstract":"<p><strong>Introduction: </strong>Biofilm is a major challenge across several sectors and contributes to serious risks to public health. This study aimed to evaluate the antibiofilm efficacy of disintegrated phages, whose lytic activities have been eliminated, against bacterial biofilms.</p><p><strong>Methods: </strong>A total of seven lytic phages were disintegrated by sonication and confirmed to have completely lost their lytic activities by the spot test. Subsequently, both the disintegrated and intact phages were tested on the biofilms produced by five different biofilm-producing bacteria. The effects of heat and proteinase K on the ability of disintegrated phages to disrupt biofilms were determined. Moreover, the structural proteins released after the disintegration of phages were screened for their presence of lipase, amylase, protease, and DNase activities. Genome analysis of all the seven phages were screened for the presence of genes encoding proteins with enzymatic activities.</p><p><strong>Results: </strong>The disintegrated phages showed more effectiveness in degrading the bacterial biofilm when compared with intact phages. The amylase test results were positive for all the seven disintegrated phages tested, confirming the presence of starch-degrading enzymes. Genomic analysis of five phages revealed the presence of genes encoding transglycosylases, amidases, and glycosaminidases, which could contribute to biofilm degradation. However, only four of these proteins were also structural proteins of phages.</p><p><strong>Conclusions: </strong>Our study demonstrated that disintegrated phages without lytic effects can still possess biofilm degrading ability, probably associated with the enzymatic activities of their structural proteins. This study showed that phages may have activities beyond lytic phage activities exhibited by their structural enzymes.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"20-31"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling and Classifying the Hidden Potential of Bacteriophages.","authors":"Martha R J Clokie, Thomas Sicheritz-Pontén","doi":"10.1089/phage.2025.7130","DOIUrl":"https://doi.org/10.1089/phage.2025.7130","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144058821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Six Novel <i>Pseudomonas aeruginosa</i> Phages: Genomic Insights and Therapeutic Potential.","authors":"Amit Rimon, Ortal Yerushalmy, Jonathan Belin, Sivan Alkalay-Oren, Lilach Gavish, Shunit Coppenhagen-Glazer, Ronen Hazan","doi":"10.1089/phage.2024.0037","DOIUrl":"https://doi.org/10.1089/phage.2024.0037","url":null,"abstract":"<p><strong>Introduction: </strong><i>Pseudomonas aeruginosa</i> is an opportunistic pathogen that causes health care-associated infections. The rise of antibiotic-resistant bacterial strains necessitates alternative treatment strategies, with bacteriophage therapy being a promising approach.</p><p><strong>Methods: </strong>Six bacteriophages were isolated from sewage samples. Phage isolation involved centrifugation, filtration, and plaque assays. The morphology of each sample was examined using transmission electron microscopy (TEM). Genomic DNA was sequenced and compared among the isolates. The phages' lytic activities were assessed using growth curve analysis.</p><p><strong>Results: </strong>The phages displayed distinct genomic characteristics, grouping into three genomic clusters. No known virulence or antibiotic resistance genes were detected, indicating their safety for therapeutic use. Taxonomic analysis identified the phages as belonging to the genera <i>Pbunavirus</i>, <i>Nipunavirus</i>, <i>Abidjanvirus</i>, and a novel genus. TEM analysis revealed their diverse morphologies. Temperate phages showed less effective lytic activities.</p><p><strong>Conclusion: </strong>Several of the isolated bacteriophages show potential as candidates for phage therapy research and could be effective against <i>P. aeruginosa</i> infections.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"32-40"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Three Lytic Bacteriophages to Overcome Multidrug-, Extensive Drug-, and Pandrug-Resistant <i>Pseudomonas aeruginosa</i>.","authors":"Marwan Mahmood Saleh, Majeed Arsheed Sabbah, Zahraa Kamel Zedan","doi":"10.1089/phage.2021.0018","DOIUrl":"10.1089/phage.2021.0018","url":null,"abstract":"<p><strong>Background: </strong>The worrisome spread of multidrug-resistant (MDR) pathogens necessitates research on nonantibiotic therapeutics. Among these therapeutics, phage treatment uses bacteriophages (phages) as alternative antimicrobial agents.</p><p><strong>Objectives: </strong>This project evaluates the lytic efficiency of phage cocktails <i>in vitro</i> versus MDR, extensive drug-resistant (XDR), and pandrug-resistant (PDR) <i>P. aeruginosa</i> isolates.</p><p><strong>Methods: </strong>We utilized host range and genetic information to generate a three-phage cocktail capable of killing multiple clinical strains of <i>P. aeruginosa</i> and examined the effectiveness of the cocktail in this study. The isolates (114) had variable resistance to 13 antibiotics. A phage-enrichment approach was used to purify the bacteriophage cocktail; a phage lysate with a high titer (5 × 10⁹ PFU/mL) was prepared and tested against 114 <i>P. aeruginosa</i> isolates.</p><p><strong>Findings: </strong>The results showed that a cocktail of three phages (MMS1, MMS2, and MMS3) could lyse <i>P. aeruginosa</i> in both planktonic liquid and dish cultures. The MMS cocktail phages were shown to be viable between 4 and 50°C at pH 4-9. A one-step growth curve showed that the MMS phages had a latent period of 15 min and a burst period of approximately 18 min based on the size of approximately 265 offspring phages per host cell. The MMS3 phage was sequenced and shown to lack genes associated with bacterial pathogenicity or antibiotic resistance.</p><p><strong>Conclusions: </strong>Notably, XDR and PDR isolates were sensitive to the phage cocktail, a prospective substitute for antibiotics that does not contribute to the growth of antibiotic resistance, suggesting that the phage cocktail might be useful for generating personalized phage therapeutics.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 4","pages":"230-240"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}