Rusudan Goliadze, Luka Kamashidze, Natia Karumidze, Sophio Rigvava, Olia Rcheulishvili, Aleksander Rcheulishvili, Marine Goderdzishvili, Ia Kusradze
{"title":"耐Cu2+离子的新型摩根氏摩根菌噬菌体vB_Mm5","authors":"Rusudan Goliadze, Luka Kamashidze, Natia Karumidze, Sophio Rigvava, Olia Rcheulishvili, Aleksander Rcheulishvili, Marine Goderdzishvili, Ia Kusradze","doi":"10.1089/phage.2024.0036","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Research on phages targeting <i>Morganella morganii</i> is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling <i>M. morganii</i> spread in the environment.</p><p><strong>Materials and methods: </strong>This study investigates the biology and genetics of the novel <i>M. morganii</i>-infecting myophage <i>vB_Mm5</i> and evaluates its stability under Cu<sup>2+</sup> exposure.</p><p><strong>Results: </strong>Phage <i>vB_Mm5</i> has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu<sup>2+</sup>. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.</p><p><strong>Conclusions: </strong><i>vB_Mm5</i> is highly distinct from other sequenced <i>M. morganii</i> phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against <i>M. morganii</i> infections.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"6 1","pages":"41-47"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060847/pdf/","citationCount":"0","resultStr":"{\"title\":\"New <i>Morganella morganii</i> Phage vB_Mm5 with Tolerance to Cu<sup>2+</sup> Ions.\",\"authors\":\"Rusudan Goliadze, Luka Kamashidze, Natia Karumidze, Sophio Rigvava, Olia Rcheulishvili, Aleksander Rcheulishvili, Marine Goderdzishvili, Ia Kusradze\",\"doi\":\"10.1089/phage.2024.0036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Research on phages targeting <i>Morganella morganii</i> is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling <i>M. morganii</i> spread in the environment.</p><p><strong>Materials and methods: </strong>This study investigates the biology and genetics of the novel <i>M. morganii</i>-infecting myophage <i>vB_Mm5</i> and evaluates its stability under Cu<sup>2+</sup> exposure.</p><p><strong>Results: </strong>Phage <i>vB_Mm5</i> has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu<sup>2+</sup>. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.</p><p><strong>Conclusions: </strong><i>vB_Mm5</i> is highly distinct from other sequenced <i>M. morganii</i> phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against <i>M. morganii</i> infections.</p>\",\"PeriodicalId\":74428,\"journal\":{\"name\":\"PHAGE (New Rochelle, N.Y.)\",\"volume\":\"6 1\",\"pages\":\"41-47\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12060847/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PHAGE (New Rochelle, N.Y.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/phage.2024.0036\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2024.0036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
New Morganella morganii Phage vB_Mm5 with Tolerance to Cu2+ Ions.
Background: Research on phages targeting Morganella morganii is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling M. morganii spread in the environment.
Materials and methods: This study investigates the biology and genetics of the novel M. morganii-infecting myophage vB_Mm5 and evaluates its stability under Cu2+ exposure.
Results: Phage vB_Mm5 has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu2+. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.
Conclusions: vB_Mm5 is highly distinct from other sequenced M. morganii phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against M. morganii infections.
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