New Morganella morganii Phage vB_Mm5 with Tolerance to Cu²⁺ Ions.

PHAGE (New Rochelle, N.Y.) Pub Date : 2025-03-17 eCollection Date: 2025-03-01 DOI:10.1089/phage.2024.0036

Rusudan Goliadze, Luka Kamashidze, Natia Karumidze, Sophio Rigvava, Olia Rcheulishvili, Aleksander Rcheulishvili, Marine Goderdzishvili, Ia Kusradze

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Abstract

Background: Research on phages targeting Morganella morganii is still emerging, with limited studies compared with other phage-host systems. Interest in these phages has increased due to rising antibiotic resistance and their potential for controlling M. morganii spread in the environment.

Materials and methods: This study investigates the biology and genetics of the novel M. morganii-infecting myophage vB_Mm5 and evaluates its stability under Cu²⁺ exposure.

Results: Phage vB_Mm5 has a 10-min latent period and a burst size of 30 (±5). It shows high tolerance to elevated temperatures and Cu²⁺. The phage genome, comprising 163,232 bp dsDNA with 229 open reading frames, encodes genes that not only enhance the phage's predatory capabilities but also confer resistance to host defense mechanisms.

Conclusions: vB_Mm5 is highly distinct from other sequenced M. morganii phages, does not contain any known virulence genes, and holds potential as a therapeutic agent against M. morganii infections.

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耐Cu2+离子的新型摩根氏摩根菌噬菌体vB_Mm5

背景：针对摩根氏摩根菌的噬菌体研究尚处于起步阶段，与其他噬菌体-宿主系统相比研究有限。对这些噬菌体的兴趣随着抗生素耐药性的增加和它们在控制莫氏分枝杆菌在环境中的传播方面的潜力而增加。材料与方法：本研究对新型morganii感染噬菌体vB_Mm5的生物学和遗传学进行了研究，并对其在Cu2+暴露下的稳定性进行了评价。结果：噬菌体vB_Mm5潜伏期为10 min，爆发大小为30（±5）。它对高温和Cu2+具有很高的耐受性。噬菌体基因组由163232 bp的dsDNA和229个开放阅读框组成，其编码的基因不仅增强了噬菌体的捕食能力，还赋予了对宿主防御机制的抵抗力。结论：vB_Mm5与其他已测序的摩根氏分枝杆菌噬菌体高度不同，不含任何已知的毒力基因，具有作为摩根氏分枝杆菌感染治疗药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PHAGE (New Rochelle, N.Y.)

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