PHAGE (New Rochelle, N.Y.)最新文献_第2页

Celebrating Progress and Overcoming Challenges in Phage Research. 庆祝噬菌体研究的进步和克服挑战。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.2908.edt

Martha Clokie, Thomas Sicheritz-Pontén

引用次数: 0

SalmoFree^® Phage Additive Proves Its Safety for Laying Hens. SalmoFree® 噬菌体添加剂证明了其对产蛋鸡的安全性。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI: 10.1089/phage.2024.0010

Santiago Hernández Villamizar, Juliana Arias Bonilla, Álvaro Hugo Jaramillo, Ricardo Piñeros, Antonio Ripoll, Laura Fonseca, Karen Riveros, Martha J Vives, Paola Barato, Viviana Clavijo

{"title":"SalmoFree® Phage Additive Proves Its Safety for Laying Hens.","authors":"Santiago Hernández Villamizar, Juliana Arias Bonilla, Álvaro Hugo Jaramillo, Ricardo Piñeros, Antonio Ripoll, Laura Fonseca, Karen Riveros, Martha J Vives, Paola Barato, Viviana Clavijo","doi":"10.1089/phage.2024.0010","DOIUrl":"10.1089/phage.2024.0010","url":null,"abstract":"Background: The avian pathogen Salmonella Gallinarum causes avian typhosis in laying hens, leading to high mortality rates among adult birds, which poses a significant problem in the poultry industry. Various products, such as vaccines, antibiotics, probiotics, and disinfectants, are commonly used to prevent and control the disease on farms. An alternative to these products is the use of bacteriophages, which may effectively prevent the colonization of S. Gallinarum.Materials and methods: This study evaluated the safety of SalmoFree®, a bacteriophage cocktail, administered to 276 laying hens from the first week of age until the 28th week. The hens were divided into two groups: a control group (138 birds) and a treatment group (138 birds). Over the 28-week period, eight doses of SalmoFree® (∼1010 UFP per bird) were administered via drinking water in a controlled environment.Results: The results indicate that the consumption of SalmoFree® has no adverse effects on bird health or zootechnical parameters. Additionally, there is a trend toward improving weight homogeneity (up to 19%), feed conversion (up to 68%), and egg weight (up to 2.7%). The detection of phages by PCR in cloacal swabs suggests that they persist in birds for 2 to 8 weeks post-ingestion. Furthermore, phages were detected in organs and eggshells, indicating that they provide protection beyond the gut.Conclusion: The study demonstrates that SalmoFree® is safe for use in laying hens and may offer additional benefits, such as improved zootechnical parameters and extended protection against S. Gallinarum through the persistence of bacteriophages in the birds.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 3","pages":"143-152"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Invade: Characterizing Phages through Their Actions and Genomes. Cartoon by Ellie Jameson. 入侵：通过噬菌体的作用和基因组来描述噬菌体的特征。漫画：Ellie Jameson。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2024.0019

引用次数: 0

Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3. 介绍分枝杆菌噬菌体 B3 亚簇成员 Casbah、Kronus 和 MmasiCarm。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2024.0004

Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia

{"title":"Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3.","authors":"Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia","doi":"10.1089/phage.2024.0004","DOIUrl":"10.1089/phage.2024.0004","url":null,"abstract":"Background: As part of a large science education effort, bacteriophages that lyse Mycobacterium smegmatis mc2155 continue to be discovered.Materials and methods: Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.Results: Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.Conclusions: Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and Gordonia DR phages.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 2","pages":"84-90"},"PeriodicalIF":0.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Biochemical Characterization of a New Phage-Encoded Muramidase, KTN6 Gp46. 一种新的噬菌体编码喃喃苷酶 KTN6 Gp46 的结构和生化特征。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2023.0040

Marta Sanz-Gaitero, Vincent De Maesschalck, Ankur Patel, Hannelore Longin, Vera Van Noort, Lorena Rodriguez-Rubio, Michael van Ryne, Katarzyna Danis-Wlodarczyk, Zuzanna Drulis-Kawa, Stephane Mesnage, Mark van Raaij, Rob Lavigne

{"title":"Structural and Biochemical Characterization of a New Phage-Encoded Muramidase, KTN6 Gp46.","authors":"Marta Sanz-Gaitero, Vincent De Maesschalck, Ankur Patel, Hannelore Longin, Vera Van Noort, Lorena Rodriguez-Rubio, Michael van Ryne, Katarzyna Danis-Wlodarczyk, Zuzanna Drulis-Kawa, Stephane Mesnage, Mark van Raaij, Rob Lavigne","doi":"10.1089/phage.2023.0040","DOIUrl":"10.1089/phage.2023.0040","url":null,"abstract":"Background: Endolysins are phage-encoded lytic enzymes that degrade bacterial peptidoglycan at the end of phage lytic cycles to release new phage particles. These enzymes are being explored as an alternative to small-molecule antibiotics.Methods: The crystal structure of KTN6 Gp46 was determined and compared with a ColabFold model. Cleavage specificity was examined using a peptidoglycan digest and reversed-phase high-performance liquid chromatography coupled to mass spectrometry (HPLC/MS).Results: The structure of KTN6 Gp46 could be determined at 1.4 Å resolution, and key differences in loops of the putative peptidoglycan binding domain were identified in comparison with its closest known homologue, the endolysin of phage SPN1S. Reversed-phase HPLC/MS analysis of the reaction products following peptidoglycan digestion confirmed the muramidase activity of Gp46, consistent with structural predictions.Conclusion: These insights into the structure and function of endolysins further expand the toolbox for endolysin engineering and explore their potential in enzyme-based antibacterial design strategies.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 2","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141908549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial June 2024. 社论 2024 年 6 月。

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI: 10.1089/phage.2024.0020

Martha Rebecca Jane Clokie, Thomas Sicheritz-Pontén

引用次数: 0

Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of Nocardia seriolae. 鱼感染诺卡菌致病性菌株NS-I的分离及全基因组测序

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-12-14 eCollection Date: 2023-12-01 DOI: 10.1089/phage.2023.0019

Muhammad Akmal, Kaho Araki, Issei Nishiki, Terutoyo Yoshida

{"title":"Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of Nocardia seriolae.","authors":"Muhammad Akmal, Kaho Araki, Issei Nishiki, Terutoyo Yoshida","doi":"10.1089/phage.2023.0019","DOIUrl":"10.1089/phage.2023.0019","url":null,"abstract":"Background: Nocardiosis, an infection caused by bacterium Nocardia seriolae, contributes to severe economic losses in the aqua-cultured fish species Seriola quinqueradiata and Seriola dumerili in Japan. Phage therapy is a potential solution for combating this infection.Materials and methods: A lytic bacteriophage infecting N. seriolae (JCM3359) was isolated from water samples collected at various fish farms. The bacteriophage was characterized by electron microscopy, host range analysis, and genomic profiling.Results: The isolated lytic bacteriophage, NS-I (accession no. OP429095.1), had a siphovirus morphotype with a broad lytic spectrum, as it infected all the tested N. seriolae strains (15/15). Its genome was a 43,361 bp double-stranded DNA molecule, exhibiting a GC content of 67% with 94% similarity to Gordonia bacteriophage Gustav at the genome level.Conclusion: Lytic bacteriophage NS-I shows promise as a biocontrol agent for nocardiosis infection in fish farms and warrants further investigation.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 4","pages":"151-158"},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride. 利用渗透休克和苯扎氯铵从噬菌体中生产泰洛菌素。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.0014

Cedric Woudstra, Lone Brøndsted

引用次数: 1

Phage Therapy in the Management of Urinary Tract Infections: A Comprehensive Systematic Review. 噬菌体治疗在尿路感染治疗中的应用：一项全面的系统综述。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.0024

Amany M Al-Anany, Payton B Hooey, Jonathan D Cook, Lori L Burrows, Julia Martyniuk, Alexander P Hynes, Greg J German

{"title":"Phage Therapy in the Management of Urinary Tract Infections: A Comprehensive Systematic Review.","authors":"Amany M Al-Anany, Payton B Hooey, Jonathan D Cook, Lori L Burrows, Julia Martyniuk, Alexander P Hynes, Greg J German","doi":"10.1089/phage.2023.0024","DOIUrl":"10.1089/phage.2023.0024","url":null,"abstract":"Urinary tract infections (UTIs) are a problem worldwide, affecting almost half a billion people each year. Increasing antibiotic resistance and limited therapeutic options have led to the exploration of alternative therapies for UTIs, including bacteriophage (phage) therapy. This systematic review aims at evaluating the efficacy of phage therapy in treating UTIs. We employed a comprehensive search strategy for any language, any animal, and any publication date. A total of 55 in vivo and clinical studies were included. Of the studies, 22% were published in a non-English language, 32.7% were before the year 1996, and the rest were after 2005. The results of this review suggest that phage therapy for UTIs can be effective; more than 72% of the included articles reported microbiological and clinical improvements. On the other hand, only 5 randomized controlled trials have been completed, and case reports and case series information were frequently incomplete for analysis. Overall, this comprehensive systematic review identifies preliminary evidence supporting the potential of phage therapy as a safe and viable option for the treatment of UTIs.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"112-127"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring Effective Phage Cocktails for Long-Term Lysis of Escherichia coli Based on Physiological Properties of Constituent Phages. 基于组成噬菌体的生理特性，定制用于大肠杆菌长期裂解的高效噬菌体鸡尾酒。

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI: 10.1089/phage.2023.0016

Tomoyoshi Kaneko, Toshifumi Osaka, Satoshi Tsuneda

{"title":"Tailoring Effective Phage Cocktails for Long-Term Lysis of Escherichia coli Based on Physiological Properties of Constituent Phages.","authors":"Tomoyoshi Kaneko, Toshifumi Osaka, Satoshi Tsuneda","doi":"10.1089/phage.2023.0016","DOIUrl":"10.1089/phage.2023.0016","url":null,"abstract":"Background: Bacteriophage (phage) therapy has regained attention as an alternative to antimicrobial agents for eliminating bacteria; however, the emergence of phage-resistant bacteria during the therapy is a major concern. One method to control this emergence is to create a cocktail composed of multiple phages.Materials and methods: In this study, we isolated 28 phages infecting Escherichia coli and evaluated their bacteriolysis (lysis) activity, lytic spectrum, adsorption rate constant, burst size, and titer of a 1-day incubation, followed by clustering of the phages based on these physiological characteristics.Results: The variation in lysis onset time and duration was more significant for cocktails of phages from different clusters than for phage cocktails from the same cluster.Conclusions: This suggests that a combination of phages with different physiological characteristics is necessary to create a cocktail that rapidly and continuously lyses bacteria over a prolonged duration while suppressing the emergence of resistant bacterial strains.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"128-135"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574527/pdf/phage.2023.0016.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0