PHAGE (New Rochelle, N.Y.)最新文献

{"title":"Producing Tailocins from Phages Using Osmotic Shock and Benzalkonium Chloride.","authors":"Cedric Woudstra,&nbsp;Lone Brøndsted","doi":"10.1089/phage.2023.0014","DOIUrl":"10.1089/phage.2023.0014","url":null,"abstract":"<p><p>In the light of the worldwide antimicrobial resistance crisis, new substitutes to antibiotics are urgently needed. Tailocins or phage tail-like bacteriocin particles, produced by bacteria for environmental competition, are a potential antimicrobial alternative to antibiotic treatment. Yet, the availability of characterized Tailocins is limited. We explored the possibility to produce new Tailocins from phage particles, using osmotic shock or chemical treatment by the ammonium quaternary compound benzalkonium chloride on <i>Ackermannviridae</i> phage S117 and using <i>Straboviridae</i> phage T4 as control. We report that phage S117 was resistant to such treatment, while successful production of Tailocins by osmotic shock was achieved for phage T4. Finally, chemical treatment with benzalkonium chloride was inefficient on phage S117 but successfully inactivated phage T4 without production of Tailocins. Further studies are needed to implement such treatments of phages for producing Tailocins with killing activity.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"136-140"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574523/pdf/phage.2023.0014.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailoring Effective Phage Cocktails for Long-Term Lysis of <i>Escherichia coli</i> Based on Physiological Properties of Constituent Phages.","authors":"Tomoyoshi Kaneko,&nbsp;Toshifumi Osaka,&nbsp;Satoshi Tsuneda","doi":"10.1089/phage.2023.0016","DOIUrl":"10.1089/phage.2023.0016","url":null,"abstract":"<p><strong>Background: </strong>Bacteriophage (phage) therapy has regained attention as an alternative to antimicrobial agents for eliminating bacteria; however, the emergence of phage-resistant bacteria during the therapy is a major concern. One method to control this emergence is to create a cocktail composed of multiple phages.</p><p><strong>Materials and methods: </strong>In this study, we isolated 28 phages infecting <i>Escherichia coli</i> and evaluated their bacteriolysis (lysis) activity, lytic spectrum, adsorption rate constant, burst size, and titer of a 1-day incubation, followed by clustering of the phages based on these physiological characteristics.</p><p><strong>Results: </strong>The variation in lysis onset time and duration was more significant for cocktails of phages from different clusters than for phage cocktails from the same cluster.</p><p><strong>Conclusions: </strong>This suggests that a combination of phages with different physiological characteristics is necessary to create a cocktail that rapidly and continuously lyses bacteria over a prolonged duration while suppressing the emergence of resistant bacterial strains.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"128-135"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574527/pdf/phage.2023.0016.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel <i>Escherichia coli</i>-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates.","authors":"Janet Yakubu Nale,&nbsp;Benjamin Chan,&nbsp;Nnaemeka Emmanuel Nnadi,&nbsp;Jeffrey Kwok Jone Cheng,&nbsp;Susan Matts,&nbsp;Neda Nezam-Abadi,&nbsp;Christopher Jason Richard Turkington,&nbsp;Lucie Manon Charreton,&nbsp;Harroop Bola,&nbsp;Ramez Nazir,&nbsp;Abubakar Shaaban Hoza,&nbsp;Samuel Posian Wamala,&nbsp;Ivan Ibanda,&nbsp;Alice Nyambura Maina,&nbsp;Auleria Ajiambo Apopo,&nbsp;Venance Theophil Msoffe,&nbsp;Nyambura Moremi,&nbsp;Grace Wanjiru Moore,&nbsp;Ismail Asiimwe,&nbsp;Alice Namatovu,&nbsp;Paul Mutumba,&nbsp;Deus Kamya,&nbsp;Ritah Nabunje,&nbsp;Immaculate Nakabugo,&nbsp;Rudovick Ruben Kazwala,&nbsp;Erastus Kangethe,&nbsp;Abel Abera Negash,&nbsp;Arthur Kalyebi Watelo,&nbsp;Nelson Bukamba,&nbsp;Gideon Muhindo,&nbsp;Nathan Musisi Lubowa,&nbsp;Ngalla Jillani,&nbsp;Atunga Nyachieo,&nbsp;George Nasinyama,&nbsp;Jesca Nakavuma,&nbsp;Andrew Millard,&nbsp;Tobi Elaine Nagel,&nbsp;Martha Rebecca Jane Clokie","doi":"10.1089/phage.2023.0012","DOIUrl":"10.1089/phage.2023.0012","url":null,"abstract":"<p><strong>Background: </strong>The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.</p><p><strong>Materials and methods: </strong>During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel <i>Escherichia coli</i> lytic phages were isolated and characterized.</p><p><strong>Results: </strong>The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera <i>Dhakavirus</i> and <i>Tunavirus</i>, respectively.</p><p><strong>Conclusion: </strong>The phages isolated have therapeutic potential for further development against <i>E. coli</i> infections.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"141-149"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574529/pdf/phage.2023.0012.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHAGE Impact and the Impact of Phages.","authors":"Martha Clokie,&nbsp;Thomas Sicheritz-Pontén","doi":"10.1089/phage.2023.29046.editorial","DOIUrl":"10.1089/phage.2023.29046.editorial","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"110"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574526/pdf/phage.2023.29046.editorial.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cooling waters: Phages to treat urinary tract infections-Phage Cartoon by Ellie Jameson.","authors":"","doi":"10.1089/phage.2023.29045.car","DOIUrl":"10.1089/phage.2023.29045.car","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"111"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574528/pdf/phage.2023.29045.car.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Correction to: In Vitro</i> Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol by Kok et al. <i>Phage (New Rochelle)</i> 2023;4(2):68-81. DOI: 10.1089/phage.2023.0005.","authors":"","doi":"10.1089/phage.2023.0005.correx","DOIUrl":"10.1089/phage.2023.0005.correx","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1089/phage.2023.0005.].</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 3","pages":"150"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574524/pdf/phage.2023.0005.correx.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a Diverse Collection of Salmonella Phages Isolated from Tennessee Wastewater.","authors":"Daniel W Bryan,&nbsp;Lauren K Hudson,&nbsp;Jia Wang,&nbsp;Thomas G Denes","doi":"10.1089/phage.2023.0004","DOIUrl":"https://doi.org/10.1089/phage.2023.0004","url":null,"abstract":"<p><strong>Background: </strong><i>Salmonella enterica</i> is one of the most prevalent bacterial foodborne pathogens. <i>Salmonella</i> phages are currently used in biocontrol applications and have potential for use as therapeutics.</p><p><strong>Materials and methods: </strong>Phages were enriched and purified from a diversity of <i>Salmonella</i> host isolates. Morphology was determined with transmission electron microscopy, host ranges were characterized using an efficiency of plaquing assay, and comparative genomic analysis was performed to determine taxonomy.</p><p><strong>Results: </strong>Ten phages were isolated and characterized. Phages showed activity against 23 out of the 24 <i>Salmonella</i> serovars evaluated. Two phages also showed activity against <i>Escherichia coli</i> strain B. Phages belonged to five different genera (<i>Ithacavirus</i>, <i>Gelderlandvirus</i>, <i>Kuttervirus</i>, <i>Tlsvirus</i>, and <i>Epseptimavirus</i>), two established species, and eight novel species.</p><p><strong>Conclusions: </strong>The phages described here further demonstrate the diversity of <i>S. enterica</i> phages present in wastewater effluent. This work contributes a collection of characterized phages from eastern Tennessee that may be of use in future phage-based applications targeting <i>S. enterica</i>.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"90-98"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two Can Play at That Game: Phage-Antibiotic Synergy-Phage Cartoon by Ellie Jameson.","authors":"","doi":"10.1089/phage.2023.29042.car","DOIUrl":"10.1089/phage.2023.29042.car","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"54"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10059530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Summer of Phage Conferences Reflects the Burgeoning Interest in Phages.","authors":"Martha R J Clokie, Thomas Sicheritz-Pontén","doi":"10.1089/phage.2023.29043.editorial","DOIUrl":"10.1089/phage.2023.29043.editorial","url":null,"abstract":"","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"53"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10059531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Evolution to Increase the Titers of Difficult Bacteriophages: RAMP-UP Protocol.","authors":"Danielle N Kok, Joanne Turnbull, Nobuto Takeuchi, Philippos K Tsourkas, Heather L Hendrickson","doi":"10.1089/phage.2023.0005","DOIUrl":"10.1089/phage.2023.0005","url":null,"abstract":"<p><strong>Background: </strong>Bacteriophages are becoming increasingly important in the race to find alternatives to antibiotics. Unfortunately, bacteriophages that might otherwise be useful are sometimes discarded due to low titers making them unsuitable for downstream applications.</p><p><strong>Methods: </strong>Here, we present two distinct approaches used to experimentally evolve novel New Zealand Paenibacillus larvae bacteriophages. The first approach uses the traditional agar-overlay method, whereas the other was a 96-well plate liquid infection protocol that improved phage titers in as little as four days. We also used a mathematical model to probe the parameters and limits of the RAMP-UP approach to rapidly select mutants that improve bacteriophage titers.</p><p><strong>Results: </strong>Both experimental approaches resulted in an increase in plaque-forming units (PFU/mL). The liquid infection approach developed here, which we call RAMP-UP for Rapid Adaptive Mutation of Phage - UP, was significantly faster and simpler, and allowed us to evolve high titer bacteriophages in as little as four days. Titers were increased from 100-100,000-fold relative to their ancestors. The resultant titers were sufficient to extract and sequence DNA from these bacteriophages. An analysis of these phage genomes is provided.</p><p><strong>Conclusion: </strong>The RAMP-UP protocol is an effective method for experimentally evolving previously intractable bacteriophages in a high-throughput and expeditious manner.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 2","pages":"68-81"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9715645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}