Identification and Characterization of Salmonella Phages and Absence of Salmonella Strains from Three Different Study Areas of Cattle Livestock.

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-12-18 eCollection Date: 2024-12-01 DOI:10.1089/phage.2023.0048

Dácil Rivera, Kasim Allel, Carla Salazar, Christopher Hamilton-West, Fernando Dueñas, Luis Pablo Hervé, Andrea I Moreno-Switt

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Abstract

Background: Salmonella represents a significant risk to both public and animal health. Although Salmonella strains have not been isolated from cattle livestock, Salmonella phages have been successfully identified.

Materials and methods: Our study was conducted through (i) investigating the presence of Salmonella strains and Salmonella phages in three study areas, (ii) phenotypic screening by lytic profile (LP); (iii) selecting nine phages for sequencing; and (iv) genomic comparison to evaluate their relative diversity.

Results: A total of 307 samples were analyzed, resulting in a total of 162 virus-like particles (VLPs) analyzed. The LP was performed to identify Salmonella strains susceptible to phage infection, including the most frequent serovars: Dublin, Enteritidis, and Javiana. From the VLPs, nine phages were selected for genomic comparison. These phages represent three morphotypes: siphoviruses, myoviruses, and podoviruses, originating from different geographic and productive sites.

Conclusions: This study enhances the understanding of the presence and diversity of Salmonella phages in cattle livestock, even in the absence of Salmonella strains.

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沙门菌噬菌体的鉴定与特征及三个不同研究区沙门菌的缺失。

背景：沙门氏菌对公众和动物健康都构成重大风险。虽然沙门氏菌还没有从牛中分离出来，但已经成功地鉴定出了沙门氏菌噬菌体。材料和方法：我们的研究通过(i)调查沙门氏菌菌株和沙门氏菌噬菌体在三个研究区域的存在，（ii）通过裂解谱（LP）进行表型筛选；（iii）选择9个噬菌体进行测序；(4)基因组比较，评价其相对多样性。结果：共分析了307份样本，分析了162个病毒样颗粒（vlp）。采用LP鉴定对噬菌体感染敏感的沙门氏菌菌株，包括最常见的血清型：Dublin、Enteritidis和Javiana。从VLPs中选择9个噬菌体进行基因组比较。这些噬菌体代表三种形态：虹膜病毒、肌病毒和足病毒，起源于不同的地理和生产地点。结论：本研究提高了对沙门菌噬菌体在没有沙门菌的情况下存在和多样性的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PHAGE (New Rochelle, N.Y.)

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