Aerosolic Application of Phages Against S. infantis on Plates and Chicken Skin.

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-09-16 eCollection Date: 2024-09-01 DOI:10.1089/phage.2023.0037

Lisa Winkelmayer, Krista Rathammer, Susanne Richter, Theres Requat, Monika Matt, Davul Ljuhar, Philippe Jäger, Friedrich Kernmauner, Seyed Naemi, Michael Dieter Mansfeld, Georg G Duscher

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Abstract

Phages are known as a promising method to combat antimicrobial resistance (AMR) in the human and veterinary sector. Use of phage aerosols enormously increases the application field, although the impact on the infectivity of phages during nebulization needs to be evaluated. In this study S. infantis was treated on plates and chicken skin with nebulized phage particles of the Myoviridae type, identified by transmission electron microscopy, using a commercial nebulizer primarily used for H₂O₂ disinfection. The reduction of bacterial number by aerosol applied phage particles was evaluated. It could clearly be shown that the phage particles were able to infect Salmonella after being nebulized using ultrasound technology. Further studies on other types of phages as well as other conditions must be performed to standardize the aerosolic application of phages.

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在平板和鸡皮上气溶胶应用噬菌体抗婴儿沙门氏菌

众所周知，噬菌体是对抗人类和兽医领域抗菌药耐药性（AMR）的有效方法。噬菌体气溶胶的使用大大拓宽了应用领域，但雾化过程中对噬菌体感染性的影响仍有待评估。本研究使用主要用于 H2O2 消毒的商用雾化器，在平板和鸡皮上用经透射电子显微镜鉴定的 Myoviridae 型噬菌体颗粒雾化处理婴儿噬菌体。对雾化噬菌体颗粒减少细菌数量的效果进行了评估。结果清楚地表明，噬菌体颗粒在使用超声波技术雾化后能够感染沙门氏菌。必须对其他类型的噬菌体和其他条件进行进一步研究，以规范噬菌体的气溶胶应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PHAGE (New Rochelle, N.Y.)

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