Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates.

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-09-01 Epub Date: 2023-09-20 DOI:10.1089/phage.2023.0012

Janet Yakubu Nale, Benjamin Chan, Nnaemeka Emmanuel Nnadi, Jeffrey Kwok Jone Cheng, Susan Matts, Neda Nezam-Abadi, Christopher Jason Richard Turkington, Lucie Manon Charreton, Harroop Bola, Ramez Nazir, Abubakar Shaaban Hoza, Samuel Posian Wamala, Ivan Ibanda, Alice Nyambura Maina, Auleria Ajiambo Apopo, Venance Theophil Msoffe, Nyambura Moremi, Grace Wanjiru Moore, Ismail Asiimwe, Alice Namatovu, Paul Mutumba, Deus Kamya, Ritah Nabunje, Immaculate Nakabugo, Rudovick Ruben Kazwala, Erastus Kangethe, Abel Abera Negash, Arthur Kalyebi Watelo, Nelson Bukamba, Gideon Muhindo, Nathan Musisi Lubowa, Ngalla Jillani, Atunga Nyachieo, George Nasinyama, Jesca Nakavuma, Andrew Millard, Tobi Elaine Nagel, Martha Rebecca Jane Clokie

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Abstract

Background: The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available.

Materials and methods: During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized.

Results: The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively.

Conclusion: The phages isolated have therapeutic potential for further development against E. coli infections.

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乌干达分离的新型大肠杆菌感染噬菌体，靶向人类临床分离株。

背景：抗微生物耐药性灾难是一个日益严重的全球健康威胁，预计发展中国家的情况会更糟。全球健康噬菌体组织（PGH）正在培训这些地区的科学家，以分离出当地致病细菌的相关治疗噬菌体，从而为噬菌体技术的普及做出贡献。材料和方法：在东非首届PGH研讨会期间，收集了乌干达城市污水处理设施的样本，分离并鉴定了两种新型大肠杆菌裂解噬菌体。结果：噬菌体，UP19（衣壳直径～100 nm，可收缩尾巴～120/20 nm）和UP30（衣壳直径～70 nm，约170/20的非实用尾部 nm），分别裂解了所检查的11个临床分离株中的～82%和～36%。UP19的基因组（171.402 kb，282 CDS）和UP30（49.834 kb，75CDS）分别与Dhakavirus属和Tunavirus属紧密匹配。结论：分离的噬菌体具有进一步开发抗大肠杆菌感染的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PHAGE (New Rochelle, N.Y.)

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