PHAGE (New Rochelle, N.Y.)最新文献_第5页

Call for Papers: PHAGE: Therapy, Applications, and Research. 征文:噬菌体:治疗、应用和研究。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-09-01 DOI: 10.1089/phage.2022.29033.cfp

Martha R J Clokie, Thomas Sicheritz-Pontén

引用次数: 0

Comparative Genomics of Six Lytic Bacillus subtilis Phages from the Southwest United States. 美国西南部6种枯草芽孢杆菌噬菌体的比较基因组学研究。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-09-01 DOI: 10.1089/phage.2022.0030

Albert C Vill, Véronique A Delesalle, Brianne E Tomko, Katherine B Lichty, Madison S Strine, Alexandra A Guffey, Elizabeth A Burton, Natalie T Tanke, Greg P Krukonis

{"title":"Comparative Genomics of Six Lytic Bacillus subtilis Phages from the Southwest United States.","authors":"Albert C Vill, Véronique A Delesalle, Brianne E Tomko, Katherine B Lichty, Madison S Strine, Alexandra A Guffey, Elizabeth A Burton, Natalie T Tanke, Greg P Krukonis","doi":"10.1089/phage.2022.0030","DOIUrl":"https://doi.org/10.1089/phage.2022.0030","url":null,"abstract":"Background: Despite their importance to microbial dynamics involving Bacillus subtilis, we have a limited understanding of the diversity of phages that can lyse this model organism.Materials and methods: Phages were isolated from soil samples collected from various sites in the southwest U.S. deserts on a wild B. subtilis strain. Their genomes were assembled, characterized, and bioinformatically compared.Results: Six Siphoviruses with high nucleotide and amino acid similarity to each other (>80%) but very limited similarity to phages currently in GenBank were isolated. These phages have double-stranded DNA genomes (55,312 to 56,127 bp) with 86-91 putative protein coding genes, and a low GC content. Comparative genomics reveal differences in loci encoding proteins that are putatively involved in bacterial adsorption with evidence for genomic mosaicism and a possible role for small genes.Conclusions: A comparative approach provides insights into phage evolution, including the role of indels in protein folding.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 3","pages":"171-178"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917325/pdf/phage.2022.0030.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10190529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mucosal Environment Induces Phage Susceptibility in Streptococcus mutans. 粘膜环境诱导变形链球菌噬菌体易感性。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-09-01 DOI: 10.1089/phage.2022.0021

Lotta-Riina Sundberg, Noora Rantanen, Gabriel Magno de Freitas Almeida

{"title":"Mucosal Environment Induces Phage Susceptibility in Streptococcus mutans.","authors":"Lotta-Riina Sundberg, Noora Rantanen, Gabriel Magno de Freitas Almeida","doi":"10.1089/phage.2022.0021","DOIUrl":"https://doi.org/10.1089/phage.2022.0021","url":null,"abstract":"Pathogenic bacteria are attracted toward mucosa, as it is their way of entry into the body. However, we know surprisingly little about the phage-bacterium interactions in the mucosal environment. Here, we explored the effect of the mucosal environment on growth characteristics and phage-bacterium interactions in Streptococcus mutans, a causative agent of dental caries. We found that although mucin supplementation increased bacterial growth and survival, it decreased S. mutans biofilm formation. More importantly, the presence of mucin had a significant effect on S. mutans phage susceptibility. In two experiments done in Brain Heart Infusion Broth, phage M102 replication was detected only with 0.2% mucin supplementation. In 0.1 × Tryptic Soy Broth, 0.5% mucin supplementation led to a 4-log increase in phage titers compared with the control. These results suggest that the mucosal environment can have a major role in the growth, phage sensitivity, and phage resistance of S. mutans, and underline the importance of understanding the effect of mucosal environment on phage-bacterium interactions.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 3","pages":"128-135"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9917281/pdf/phage.2022.0021.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10190530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Setting Up a Better Infection: Overexpression of the Early Bacteriophage T4 Gene motB During Infection Results in a More Favorable tRNA Pool for the Phage. 建立更好的感染:在感染过程中，早期噬菌体T4基因motB的过表达导致噬菌体有更有利的tRNA池。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-09-01 Epub Date: 2022-09-19 DOI: 10.1089/phage.2022.0023

Bokyung Son, Jennifer Patterson-West, Christine O Thompson, James R Iben, Deborah M Hinton

{"title":"Setting Up a Better Infection: Overexpression of the Early Bacteriophage T4 Gene motB During Infection Results in a More Favorable tRNA Pool for the Phage.","authors":"Bokyung Son, Jennifer Patterson-West, Christine O Thompson, James R Iben, Deborah M Hinton","doi":"10.1089/phage.2022.0023","DOIUrl":"https://doi.org/10.1089/phage.2022.0023","url":null,"abstract":"Background: : Although many bacteriophage T4 early genes are nonessential with unknown functions, they are believed to aid in the takeover of the Escherichia coli host. Understanding the functions of these genes could be helpful to develop novel antibacterial strategies. MotB, encoded by a previously uncharacterized T4 early gene, is a DNA-binding protein that compacts the host nucleoid and alters host gene expression.Methods: : MotB structure was predicted by AlphaFold 2. RNA-seq and mass spectrometry (MS) analyses were performed to determine RNA and protein changes when motB was overexpressed in E. coli BL21(DE3) ±5 min T4 infection.Results: : MotB structure is predicted to be a two-domain protein with N-terminal Kyprides-Onzonis-Woese and C-terminal oligonucleotide/oligosaccharide-fold domains. In E. coli B, motB overexpression during infection does not affect T4 RNAs, but affects the expression of host genes, including the downregulation of 21 of the 84 chargeable host tRNAs. Many of these tRNAs are used less frequently by T4 or have a counterpart encoded within the T4 genome. The MS analyses indicate that the levels of multiple T4 proteins are changed by motB overexpression.Conclusion: : Our results suggest that in this E. coli B host, motB is involved in establishing a more favorable tRNA pool for the phage during infection.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":" ","pages":"141-152"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527043/pdf/phage.2022.0023.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33489061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriophage Dark Matter - Phage Cartoon by Ellie Jameson. 噬菌体暗物质-噬菌体卡通艾莉·詹姆森。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-09-01 DOI: 10.1089/phage.2022.29035.car

引用次数: 0

RNA Management During T7 Infection. T7感染期间的RNA管理。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-09-01 DOI: 10.1089/phage.2022.0029

Aline Tabib-Salazar, Sivaramesh Wigneshweraraj

引用次数: 0

Phage Therapy: Insights from the Past, the Great Need of the Present, and Glimpses into the Future. 噬菌体治疗:从过去的洞察，现在的巨大需求，以及对未来的一瞥。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-06-01 DOI: 10.1089/phage.2022.29031.mcl

Martha R J Clokie, Thomas E Sicheritz-Pontén

引用次数: 1

Further Considerations on How to Improve Phage Therapy Experimentation, Practice, and Reporting: Pharmacodynamics Perspectives. 关于如何改进噬菌体疗法实验、实践和报告的进一步思考：药效学视角。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-06-01 Epub Date: 2022-06-16 DOI: 10.1089/phage.2022.0019

Stephen T Abedon

引用次数: 0

Professor Giorgi Eliava and the Eliava Institute of Bacteriophage. Giorgi Eliava教授和Eliava噬菌体研究所。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-06-01 DOI: 10.1089/phage.2022.0016

Nina Chanishvili, Dmitriy Myelnikov, Timothy K Blauvelt

{"title":"Professor Giorgi Eliava and the Eliava Institute of Bacteriophage.","authors":"Nina Chanishvili, Dmitriy Myelnikov, Timothy K Blauvelt","doi":"10.1089/phage.2022.0016","DOIUrl":"https://doi.org/10.1089/phage.2022.0016","url":null,"abstract":"In the history of medicine little is known about Prof. Giorgi (George) Eliava, who must be recognized as one of the central figures in the story of bacteriophages. Today it may be said without any exaggeration that without the support that Eliava provided to Felix d'Herelle, much of our knowledge about phage therapy would never have been acquired. Eliava played a central role in developing and promoting therapeutic uses of bacteriophages in the Union of Soviet Socialist Republics and beyond, and it was largely due to his efforts-and the institute that he established-that phage therapy survived in Soviet Georgia during the Cold War when it was largely abandoned in the West. Because of his progressive thinking, tireless activities, and close collaborations with many foreign scientists, including d'Herelle, Eliava became a victim of Stalin's regime in 1937, declared an \"Enemy of the People\" and executed. Most photographs and documents belonging to Eliava were destroyed by the Committee for State Security (Komitet gosudarstvennoi bezopasnosti), so it is difficult to ascertain many of the details of his life, and his scientific activities are largely unknown. His memory was restored only after the reassessment of the outcomes of the Great Terror and Stalin's regime in later periods. In this article, we bring the remarkable but little-known story of Giorgi Eliava to a wide readership and thus pay tribute to his talent and his dedication to science, and celebrate his contributions to phage research and phage therapy.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 2","pages":"71-80"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9436386/pdf/phage.2022.0016.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9912786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Discovery of the First Lytic Staphylococcus pseudintermedius/Staphylococcus aureus Polyvalent Bacteriophages. 发现首个溶解性假中间葡萄球菌/金黄色葡萄球菌多价噬菌体。

PHAGE (New Rochelle, N.Y.) Pub Date : 2022-06-01 Epub Date: 2022-06-16 DOI: 10.1089/phage.2022.0004

Haroldo Hernandez Santos, Justin Clark, Austen Terwilliger, Anthony Maresso

{"title":"Discovery of the First Lytic Staphylococcus pseudintermedius/Staphylococcus aureus Polyvalent Bacteriophages.","authors":"Haroldo Hernandez Santos, Justin Clark, Austen Terwilliger, Anthony Maresso","doi":"10.1089/phage.2022.0004","DOIUrl":"10.1089/phage.2022.0004","url":null,"abstract":"Background: There are no verified lytic Staphylococcus pseudintermedius phages in the literature and few temperate phage genomes in databases. S. pseudintermedius is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.Materials and methods: We discovered phages against canine-derived S. pseudintermedius isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.Results: Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are S. pseudintermedius and Staphylococcus aureus polyvalent phages, with DH2 and DS10 being strong killers of both species.Conclusions: We report discovery of the first verified lytic S. pseudintermedius phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-Staphylococcal phage cocktail.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"3 2","pages":"116-124"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248872/pdf/phage.2022.0004.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9912784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0