Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of Nocardia seriolae.

PHAGE (New Rochelle, N.Y.) Pub Date : 2023-12-14 eCollection Date: 2023-12-01 DOI:10.1089/phage.2023.0019

Muhammad Akmal, Kaho Araki, Issei Nishiki, Terutoyo Yoshida

{"title":"Isolation and Complete Genome Sequencing of NS-I, a Lytic Bacteriophage Infecting Fish Pathogenic Strains of Nocardia seriolae.","authors":"Muhammad Akmal, Kaho Araki, Issei Nishiki, Terutoyo Yoshida","doi":"10.1089/phage.2023.0019","DOIUrl":null,"url":null,"abstract":"Background: Nocardiosis, an infection caused by bacterium Nocardia seriolae, contributes to severe economic losses in the aqua-cultured fish species Seriola quinqueradiata and Seriola dumerili in Japan. Phage therapy is a potential solution for combating this infection.Materials and methods: A lytic bacteriophage infecting N. seriolae (JCM3359) was isolated from water samples collected at various fish farms. The bacteriophage was characterized by electron microscopy, host range analysis, and genomic profiling.Results: The isolated lytic bacteriophage, NS-I (accession no. OP429095.1), had a siphovirus morphotype with a broad lytic spectrum, as it infected all the tested N. seriolae strains (15/15). Its genome was a 43,361 bp double-stranded DNA molecule, exhibiting a GC content of 67% with 94% similarity to Gordonia bacteriophage Gustav at the genome level.Conclusion: Lytic bacteriophage NS-I shows promise as a biocontrol agent for nocardiosis infection in fish farms and warrants further investigation.","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"4 4","pages":"151-158"},"PeriodicalIF":0.0000,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932517/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2023.0019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Nocardiosis, an infection caused by bacterium Nocardia seriolae, contributes to severe economic losses in the aqua-cultured fish species Seriola quinqueradiata and Seriola dumerili in Japan. Phage therapy is a potential solution for combating this infection.

Materials and methods: A lytic bacteriophage infecting N. seriolae (JCM3359) was isolated from water samples collected at various fish farms. The bacteriophage was characterized by electron microscopy, host range analysis, and genomic profiling.

Results: The isolated lytic bacteriophage, NS-I (accession no. OP429095.1), had a siphovirus morphotype with a broad lytic spectrum, as it infected all the tested N. seriolae strains (15/15). Its genome was a 43,361 bp double-stranded DNA molecule, exhibiting a GC content of 67% with 94% similarity to Gordonia bacteriophage Gustav at the genome level.

Conclusion: Lytic bacteriophage NS-I shows promise as a biocontrol agent for nocardiosis infection in fish farms and warrants further investigation.

查看原文本刊更多论文

鱼感染诺卡菌致病性菌株NS-I的分离及全基因组测序

背景：诺卡菌病是由诺卡菌引起的一种感染，在日本造成了严重的水产养殖鱼类的经济损失。噬菌体治疗是对抗这种感染的潜在解决方案。材料与方法：从各养鱼场采集的水样中分离到一株感染梭状奈瑟菌的裂解噬菌体（JCM3359）。通过电子显微镜、宿主范围分析和基因组图谱对噬菌体进行了表征。结果：分离到的裂解噬菌体ns - 1(鉴定号：ns - 1；OP429095.1)，具有广泛的裂解谱的siphovirus形态型，因为它感染了所有测试的seriolae菌株（15/15）。其基因组为43,361 bp的双链DNA分子，GC含量为67%，与Gordonia噬菌体Gustav在基因组水平上相似度为94%。结论：裂解噬菌体ns - 1作为诺卡菌病的生物防治剂在养鱼场具有广阔的应用前景，值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

PHAGE (New Rochelle, N.Y.)

自引率

0.00%

发文量