Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia
{"title":"介绍分枝杆菌噬菌体 B3 亚簇成员 Casbah、Kronus 和 MmasiCarm。","authors":"Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia","doi":"10.1089/phage.2024.0004","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>As part of a large science education effort, bacteriophages that lyse <i>Mycobacterium smegmatis</i> mc<sup>2</sup>155 continue to be discovered.</p><p><strong>Materials and methods: </strong>Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.</p><p><strong>Results: </strong>Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.</p><p><strong>Conclusions: </strong>Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and <i>Gordonia</i> DR phages.</p>","PeriodicalId":74428,"journal":{"name":"PHAGE (New Rochelle, N.Y.)","volume":"5 2","pages":"84-90"},"PeriodicalIF":0.0000,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304909/pdf/","citationCount":"0","resultStr":"{\"title\":\"Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3.\",\"authors\":\"Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia\",\"doi\":\"10.1089/phage.2024.0004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>As part of a large science education effort, bacteriophages that lyse <i>Mycobacterium smegmatis</i> mc<sup>2</sup>155 continue to be discovered.</p><p><strong>Materials and methods: </strong>Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.</p><p><strong>Results: </strong>Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.</p><p><strong>Conclusions: </strong>Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and <i>Gordonia</i> DR phages.</p>\",\"PeriodicalId\":74428,\"journal\":{\"name\":\"PHAGE (New Rochelle, N.Y.)\",\"volume\":\"5 2\",\"pages\":\"84-90\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304909/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PHAGE (New Rochelle, N.Y.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/phage.2024.0004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PHAGE (New Rochelle, N.Y.)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/phage.2024.0004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3.
Background: As part of a large science education effort, bacteriophages that lyse Mycobacterium smegmatis mc2155 continue to be discovered.
Materials and methods: Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.
Results: Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.
Conclusions: Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and Gordonia DR phages.
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