Introducing Casbah, Kronus, and MmasiCarm, Members of the Mycobacteriophage Subcluster B3.

PHAGE (New Rochelle, N.Y.) Pub Date : 2024-06-21 eCollection Date: 2024-06-01 DOI:10.1089/phage.2024.0004

Véronique A Delesalle, Ruusu E Ankeriasniemi, Colin M Lewis, Jehan M Mody, Abigail M Roy, Ward A Sarvis, Duy D Vo, Allison E Walsh, Rose J Zappia

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Abstract

Background: As part of a large science education effort, bacteriophages that lyse Mycobacterium smegmatis mc²155 continue to be discovered.

Materials and methods: Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.

Results: Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.

Conclusions: Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and Gordonia DR phages.

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介绍分枝杆菌噬菌体 B3 亚簇成员 Casbah、Kronus 和 MmasiCarm。

背景：作为大型科学教育活动的一部分，不断发现能溶解烟曲霉分枝杆菌 mc2155 的噬菌体：从美国东北部城市的土壤样本中分离出噬菌体。对它们的基因组进行了测序、组装和生物信息学比较：结果：分离出三种属于 B3 亚群的溶解性虹吸病毒，它们与其他 B3 分枝杆菌噬菌体之间具有高度相似性。这些噬菌体含有双链 DNA 基因组（68,754 至 69,495 bp），GC 含量高（67.4%-67.5%），有 102-104 个假定的蛋白质编码基因。显著特点包括一种类似 HicA 的毒素和 33 个 B3 亚群独有的基因。一种噬菌体的终止酶序列中含有一个内切蛋白：结论：对这些噬菌体的基因组分析为基因组进化和水平基因转移（HGT）提供了见解。HGT 网络显然非常庞大，而且具有基因特异性。有趣的是，在 B3 和 Gordonia DR 噬菌体中都发现了一些基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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PHAGE (New Rochelle, N.Y.)

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