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DLGR-1, a homolog of vertebrate DLGAP proteins, regulates spindle length and anaphase velocity during C. elegans meiosis. DLGR-1是脊椎动物DLGAP蛋白的同源物，它在秀丽隐杆线虫减数分裂过程中调节纺锤体长度和无丝分裂速度。
microPublication biology Pub Date : 2024-08-16 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001305
Meghana Mahantesh Magadum, Francis McNally
{"title":"DLGR-1, a homolog of vertebrate DLGAP proteins, regulates spindle length and anaphase velocity during <i>C. elegans</i> meiosis.","authors":"Meghana Mahantesh Magadum, Francis McNally","doi":"10.17912/micropub.biology.001305","DOIUrl":"10.17912/micropub.biology.001305","url":null,"abstract":"<p><p>Chromosome segregation requires a large number of microtubule-binding proteins that mediate spindle assembly and function during mitosis and meiosis. BLAST revealed a single <i>C. elegans</i> homolog of HURP/DLGAP5, a microtubule-binding protein that regulates mitotic and meiotic spindles in vertebrates. This homolog, W03A5.6 , was named DLGR-1 (DLGAP related). Time-lapse imaging of an endogenously tagged DLGR-1::GFP during <i>C. elegans</i> meiosis revealed plasma membrane localization specifically during anaphase I and anaphase II when the meiotic spindle is closely apposed to the plasma membrane. Time-lapse imaging of microtubules and chromosomes during meiosis in a strain with a CRISPR deletion of the DLGR-1 coding sequence revealed metaphase spindles that were significantly shorter than controls and chromosome separation velocities that were significantly slower than controls. Extrusion of chromosomes into polar bodies proceeded normally, consistent with the high progeny viability of the homozygous deletion strain. Thus DLGR-1 may play an accessory or redundant role in meiotic spindle function during <i>C. elegans</i> meiosis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Silencing of a NRDE-3 transgene in C. elegans germ cells and early embryos is mediated by the RNAi pathway. 通过 RNAi 途径抑制秀丽隐杆线虫生殖细胞和早期胚胎中的 NRDE-3 转基因。
microPublication biology Pub Date : 2024-08-16 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001308
Shihui Chen, Carolyn M Phillips
{"title":"Silencing of a NRDE-3 transgene in <i>C. elegans</i> germ cells and early embryos is mediated by the RNAi pathway.","authors":"Shihui Chen, Carolyn M Phillips","doi":"10.17912/micropub.biology.001308","DOIUrl":"10.17912/micropub.biology.001308","url":null,"abstract":"<p><p>Transgenes are highly susceptible to gene silencing in the <i>C. elegans</i> germline. Here, we examine the expression of the nuclear Argonaute protein NRDE-3 , comparing two GFP::NRDE-3 strains, one constructed by bombardment and one by CRISPR. We found that the GFP::NRDE-3 strain constructed by bombardment displays transgene silencing in germline and early embryos and that NRDE-3 expression can be restored in a <i>rde-3</i> mutant, which disrupts the RNAi pathway. This finding reveals that NRDE-3 is not a soma-specific Argonaute protein and is, in fact, expressed in the proximal germline and early embryos.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of a highly specific and potent antibody against Ce-lamin/LMN-1. 生成针对 Ce-lamin/LMN-1 的高度特异性强效抗体。
microPublication biology Pub Date : 2024-08-15 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001294
Adam Zahand, Byron Williams, Jun Liu
{"title":"Generation of a highly specific and potent antibody against Ce-lamin/LMN-1.","authors":"Adam Zahand, Byron Williams, Jun Liu","doi":"10.17912/micropub.biology.001294","DOIUrl":"10.17912/micropub.biology.001294","url":null,"abstract":"<p><p>High quality antibodies are useful tools for research in cell and developmental biology. We have obtained highly specific and potent guinea pig-derived polyclonal antibodies against Ce-lamin/ LMN-1 . Western blotting experiments using these antibodies demonstrated that maternally loaded Ce-lamin/ LMN-1 protein is very stable, can perdure through embryonic and larval development and remain detectable in homozygous null <i>lmn-1</i> mutant adults.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nuclear localization of Pretaporter in Drosophila melanogaster third-instar larvae salivary gland and its deficiency-associated nuclear phenotype. 黑腹果蝇三龄幼虫唾液腺中 Pretaporter 的核定位及其缺失相关的核表型
microPublication biology Pub Date : 2024-08-13 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001246
María Constanza Silvera, María José Ferreiro
{"title":"Nuclear localization of Pretaporter in <i>Drosophila melanogaster</i> third-instar larvae salivary gland and its deficiency-associated nuclear phenotype.","authors":"María Constanza Silvera, María José Ferreiro","doi":"10.17912/micropub.biology.001246","DOIUrl":"https://doi.org/10.17912/micropub.biology.001246","url":null,"abstract":"<p><p>The <i>Drosophila melanogaster</i> protein Pretaporter, is thought to reside in the endoplasmic reticulum and relocate to the plasma membrane during apoptosis. However, very little is known about its subcellular distribution in different cell types and conditions. Here, we present the first report of Pretaporter´s subcellular distribution in the salivary gland cells of <i>Drosophila</i> third-instar larvae, finding it enriched in cell membranes, apical granules, and unexpectedly within cell nuclei. Pretaporter loss-of-function mutants exhibited hypotrophied nuclei, suggesting a potential role in DNA endoreplication control. These findings prompt a reevaluation of Pretaporter's functions and encourage future research aimed at unraveling novel roles.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11350429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Partial in-vitro dispersal of S. mutans UA159 biofilms by silver-(I)cyanoximate compounds. 银-(I)氰氧化合物在体外部分驱散变异单胞菌 UA159 生物膜。
microPublication biology Pub Date : 2024-08-12 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001262
Brendaliz Santiago Narvaez, Sarah Hameer, Jamie L Perry, Tiffany Rojas, Laurel G Habgood
{"title":"Partial in-vitro dispersal of <i>S. mutans</i> UA159 biofilms by silver-(I)cyanoximate compounds.","authors":"Brendaliz Santiago Narvaez, Sarah Hameer, Jamie L Perry, Tiffany Rojas, Laurel G Habgood","doi":"10.17912/micropub.biology.001262","DOIUrl":"10.17912/micropub.biology.001262","url":null,"abstract":"<p><p>Silver(I) cyanoximate compounds have antibacterial activity against the oral pathogen <i>Streptococcus mutans,</i> a resident of oral plaque biofilm. As oral biofilm strategies focus on the inhibition of attachment or physical removal of the existing microbes, we were interested in exploring the ability of six different silver(I) cyanoximate compounds to target and disperse a pre-existing biofilm. Here we report that these compounds were only able to partially disperse <i>S. mutans</i> biofilms as the compounds were more effective at inhibiting biofilm formation. None of the six compounds were able to outperform silver nitrate, a commonly used antibacterial in dentistry.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Atypical soluble guanylyl cyclases control brain size in Drosophila. 非典型可溶性鸟苷酸环化酶控制果蝇的大脑大小
microPublication biology Pub Date : 2024-08-11 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001252
Daniel Prieto, Boris Egger, Rafael Cantera
{"title":"Atypical soluble guanylyl cyclases control brain size in <i>Drosophila</i>.","authors":"Daniel Prieto, Boris Egger, Rafael Cantera","doi":"10.17912/micropub.biology.001252","DOIUrl":"10.17912/micropub.biology.001252","url":null,"abstract":"<p><p>Hypoxia-induced proliferation of neural stem cells has a crucial role in brain development. In the brain of <i>Drosophila melanogaster</i> , the optic lobe exhibits progressive hypoxia during larval development. Here, we investigate an alternative oxygen-sensing mechanism within this brain compartment, distinct from the canonical hypoxia signaling pathway mediated by HIF. Using genetic tools, immunostaining, and confocal microscopy, we demonstrate that the loss of the atypical soluble guanylyl cyclase (asGC) subunit <i>Gyc88E</i> , or the ectopic expression of <i>Gyc89Db</i> in neural stem cells leads to increased optic lobe volume. We propose the existence of a link between cGMP signaling and neurogenesis in the developing brain.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11344882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An RNAi screen for ribosome biogenesis genes required for Drosophila border cell collective migration. 通过 RNAi 筛选果蝇边界细胞集体迁移所需的核糖体生物基因。
microPublication biology Pub Date : 2024-08-10 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001292
Emily Burghardt, Jocelyn A McDonald
{"title":"An RNAi screen for ribosome biogenesis genes required for <i>Drosophila</i> border cell collective migration.","authors":"Emily Burghardt, Jocelyn A McDonald","doi":"10.17912/micropub.biology.001292","DOIUrl":"10.17912/micropub.biology.001292","url":null,"abstract":"<p><p>Ribosome biogenesis is critical for the proper production of proteins in cells and has emerged as a regulator of cell invasion and migration in development and in cancer. The <i>Drosophila</i> border cells form a collective that invades and migrates through the surrounding tissue during oogenesis. We previously found that a significant number of ribosome biogenesis genes are differentially expressed from early to late migration stages. Here, we performed a small-scale RNAi screen of a subset of these ribosome genes. Knockdown of seven genes disrupted border cell migration, thus revealing a role for ribosome biogenesis genes in regulating collective cell migration.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11344226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reciprocal restriction fragment length polymorphism (RFLP) analysis reveals mitochondrial heteroplasmy in Caenorhabditis briggsae hybrids. 对等限制性片段长度多态性（RFLP）分析揭示了Caenorhabditis briggsae杂交种的线粒体异型性。
microPublication biology Pub Date : 2024-08-09 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001306
Kevin Helwick, Joseph Ross
{"title":"Reciprocal restriction fragment length polymorphism (RFLP) analysis reveals mitochondrial heteroplasmy in <i>Caenorhabditis briggsae</i> hybrids.","authors":"Kevin Helwick, Joseph Ross","doi":"10.17912/micropub.biology.001306","DOIUrl":"10.17912/micropub.biology.001306","url":null,"abstract":"<p><p>Although mitochondria are typically inherited maternally, exceptions exist. We previously demonstrated that within-species crosses of <i>Caenorhabditis briggsae</i> result in paternal mitochondrial transmission, and it would be useful to know whether hybrids have only paternal mitochondria (homoplasmy) or paternal and maternal mitochondria (heteroplasmy). We developed a reciprocal restriction fragment length polymorphism analysis to separately detect paternal and maternal mitochondrial DNA. Using new hybrid lines, this approach revealed that some hybrids are heteroplasmous and others have become homoplasmous for the paternal mitotype. These results motivate additional investigation of how paternal mitochondrial transmission is apparently facile in <i>C. briggsae</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11344225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polyethylene glycol as an improved barrier to prevent fleeing in C. elegans. 聚乙二醇作为一种改进的屏障，可防止 elegans 的逃逸。
microPublication biology Pub Date : 2024-08-08 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001288
Safa Beydoun, Aditya Sridhar, Mira Bhandari, Elizabeth S Kitto, Scott F Leiser
{"title":"Polyethylene glycol as an improved barrier to prevent fleeing in <i>C. elegans</i>.","authors":"Safa Beydoun, Aditya Sridhar, Mira Bhandari, Elizabeth S Kitto, Scott F Leiser","doi":"10.17912/micropub.biology.001288","DOIUrl":"10.17912/micropub.biology.001288","url":null,"abstract":"<p><p><i>Caenorhabditis elegans</i> studies can be constrained by worms escaping standard solid nematode growth medium (NGM) plates. When worms are in search of food or are avoiding pathogens, chemicals, and environmental stressors, they often exhibit a behavior known as \"fleeing\". Palmitic acid (PA) is sometimes used as a barrier \"fence\" to reduce fleeing under limited food and oxygen conditions. Here, we evaluate the efficacy of palmitic acid, polyethylene glycol (PEG) and copper as potential barriers to reduce fleeing under various environmental conditions. Our results indicate that PA and PEG each reduce fasted flee rate and do not obviously alter overall health and lifespan of the worms, while copper blunts worm growth and development. We also find that PEG is a more optimal tool than PA since it is more effective in fasted conditions, reduces flee rate in a pathogenic environment, and does not alter worm size.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Naming internal insertion alleles created using CRISPR in Saccharomyces cerevisiae. 为利用 CRISPR 技术在酿酒酵母中创建的内部插入等位基因命名。
microPublication biology Pub Date : 2024-08-08 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001258
Xheni Mucelli, Linda S Huang
{"title":"Naming internal insertion alleles created using CRISPR in <i>Saccharomyces cerevisiae</i>.","authors":"Xheni Mucelli, Linda S Huang","doi":"10.17912/micropub.biology.001258","DOIUrl":"10.17912/micropub.biology.001258","url":null,"abstract":"<p><p>The budding yeast <i>Saccharomyces cerevisiae</i> is a powerful model organism, partly because of the ease of genome alterations due to the combination of a fast generation time and many molecular genetic tools. Recent advances in CRISPR-based systems allow for the easier creation of alleles with internally inserted sequences within the coding regions of genes, such as the internal insertion of sequences that code for epitopes or fluorescent proteins. Here we briefly summarize some exisiting nomenclature standards and suggest nomenclature guidelines for internal insertion alleles which are informative, consistent, and computable.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11342080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142057510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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