Sophia Mihalatos, Kevin Tompkins, Victoria Busch, Olivia Fanuele, Nuranjalie Outar, Aaron N Saran, Pukhraj Kaur, Daniel Koulta, Gurninder Ahluwalia, Vanessa Froehlich, Stephanie Lee, Sicong Wang, Dafang F Wang
{"title":"Examination of gene expression in <i>Mu</i> insertion alleles of <i>oil yellow1</i> during vegetative development in <i>Zea mays</i>.","authors":"Sophia Mihalatos, Kevin Tompkins, Victoria Busch, Olivia Fanuele, Nuranjalie Outar, Aaron N Saran, Pukhraj Kaur, Daniel Koulta, Gurninder Ahluwalia, Vanessa Froehlich, Stephanie Lee, Sicong Wang, Dafang F Wang","doi":"10.17912/micropub.biology.001643","DOIUrl":null,"url":null,"abstract":"<p><p>The <i>oil yellow1</i> ( <i>oy1</i> ) gene encodes the I subunit of magnesium chelatase, a key enzyme in chlorophyll biosynthesis in Z <i>ea mays</i> . Using Uniform <i>Mu</i> insertion lines, we examined how <i>Mu</i> insertions influence OY1 expression across vegetative development. Transcription remained unchanged during juvenile stages (V3-V5) but was consistently upregulated in adult stages (V7-V9). Related genes CHLD1 and CHLH1 showed similar patterns. Although transposon insertions often disrupt gene expression, our findings show they can also enhance transcription in a stage-specific manner.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12365686/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001643","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The oil yellow1 ( oy1 ) gene encodes the I subunit of magnesium chelatase, a key enzyme in chlorophyll biosynthesis in Z ea mays . Using Uniform Mu insertion lines, we examined how Mu insertions influence OY1 expression across vegetative development. Transcription remained unchanged during juvenile stages (V3-V5) but was consistently upregulated in adult stages (V7-V9). Related genes CHLD1 and CHLH1 showed similar patterns. Although transposon insertions often disrupt gene expression, our findings show they can also enhance transcription in a stage-specific manner.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
