发布求助
文献互助 智能选刊 最新文献

microPublication biology最新文献

筛选
英文 中文
The impact of IgSF11-PKM2 pathway on gene expression in osteoclasts.
microPublication biology Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001469
Hyunsoo Kim, Noriko Takegahara, Yongwon Choi
{"title":"The impact of IgSF11-PKM2 pathway on gene expression in osteoclasts.","authors":"Hyunsoo Kim, Noriko Takegahara, Yongwon Choi","doi":"10.17912/micropub.biology.001469","DOIUrl":"https://doi.org/10.17912/micropub.biology.001469","url":null,"abstract":"<p><p>Osteoclasts are primary bone resorbing cells. Previously, we described metabolic regulation of osteoclasts through IgSF11-mediated phosphorylation of the glycolytic enzyme PKM2. Here, we report the impact of IgSF11-PKM2-mediated regulation on gene expression in osteoclasts, utilizing RNA sequencing on osteoclasts engineered to express a chimeric protein, lacking IgSF11, and pharmacologically modulating PKM2 activity. Our analysis identified osteoclast-related genes whose expression is altered by the absence of IgSF11 and by changes in PKM2 activity. This study reveals gene expression changes associated with the IgSF11-PKM2 pathway, providing new insight into its role in osteoclasts.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143804955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adaptability in the aperiodic Drosophila populations and evolution of the life-history traits.
microPublication biology Pub Date : 2025-03-23 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001436
Khushboo Sharma, Nalini Mishra, Mallikarjun N Shakarad
{"title":"Adaptability in the aperiodic <i>Drosophila</i> populations and evolution of the life-history traits.","authors":"Khushboo Sharma, Nalini Mishra, Mallikarjun N Shakarad","doi":"10.17912/micropub.biology.001436","DOIUrl":"https://doi.org/10.17912/micropub.biology.001436","url":null,"abstract":"<p><p>Exposure of diurnal animals to constant light for extended periods dampens the clock gene circadian rhythms, which in turn affect the life history traits. However, animals are expected to maintain some form of rhythm for the body to function effectively. In this study, we used three populations of <i>Drosophila melanogaster</i> that were maintained under constant light for 312 generations. We entrained three other populations (derived from those in constant light for 312 generations) to 12L:12D cycles. The adaptive evolution under aperiodic conditions has compromised on longevity and fecundity- two most important fitness traits for an iteroparous species.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation of MAP Kinase signaling by the insulin-like growth factor pathway during C. elegans vulval development.
microPublication biology Pub Date : 2025-03-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001557
Matthew Eroglu, W Brent Derry
{"title":"Regulation of MAP Kinase signaling by the insulin-like growth factor pathway during <i>C. elegans</i> vulval development.","authors":"Matthew Eroglu, W Brent Derry","doi":"10.17912/micropub.biology.001557","DOIUrl":"10.17912/micropub.biology.001557","url":null,"abstract":"<p><p>Organ development depends on multiple signaling pathways working in concert to specify cell fates. Improper activity or inactivity of specific signaling pathways such as EGF-Ras-MAPK can lead to dedifferentiation and cancer. In <i>C. elegans</i> , gain of function mutations in Ras/ <i>let-60</i> lead to ectopic development of multiple ventral vulva-like lesions resembling tumors. However, this phenotype depends on normal insulin-like growth factor (IGF) signaling. Here, we probe how factors downstream of the IGF receptor <i>daf-2</i> modify Ras signaling. These investigations led us to identify regulators of cell fate such as the Zinc finger protein encoding gene <i>mstr-1</i> ( <i>F22D6.2</i> ), homologous to mammalian <i>Zfand3</i> / <i>5</i> / <i>6</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neopterin extends C. elegans lifespan in an ATFS-1-dependent manner.
microPublication biology Pub Date : 2025-03-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001543
Miina Pitkänen, Geoffray Monteuuis, Christopher B Jackson, Olli Matilainen
{"title":"Neopterin extends <i>C. elegans</i> lifespan in an ATFS-1-dependent manner.","authors":"Miina Pitkänen, Geoffray Monteuuis, Christopher B Jackson, Olli Matilainen","doi":"10.17912/micropub.biology.001543","DOIUrl":"10.17912/micropub.biology.001543","url":null,"abstract":"<p><p>Neopterin, a byproduct of tetrahydrobiopterin synthesis, is commonly used as a biomarker for immune system activation. In addition to its role in immune responses, neopterin levels are known to increase with age. Its impact on longevity, however, remains unclear. Here, we demonstrate that neopterin supplementation extends lifespan in <i>Caenorhabditis elegans .</i> Additionally, neopterin shows moderate activation of the mitochondrial unfolded protein response (UPR <sup>mt</sup> ), and that the neopterin-mediated lifespan extension is dependent on ATFS-1 , the primary transcription factor regulating UPR <sup>mt</sup> . These findings highlight the need for further investigation into the biological functions and health-promoting effects of neopterin.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Disruption of the EmrAB-TolC efflux pump of Escherichia coli induces global metabolic changes in multiple growth conditions.
microPublication biology Pub Date : 2025-03-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001437
Dana E Harmon, Klay Adamson, Joseph Wiersma
{"title":"Disruption of the EmrAB-TolC efflux pump of <i>Escherichia coli</i> induces global metabolic changes in multiple growth conditions.","authors":"Dana E Harmon, Klay Adamson, Joseph Wiersma","doi":"10.17912/micropub.biology.001437","DOIUrl":"10.17912/micropub.biology.001437","url":null,"abstract":"<p><p>Multidrug efflux pumps are transporters that are important for the removal of exogenous toxic molecules in bacteria. Recently, efflux pumps have been implicated in the regulation of metabolic homeostasis in <i>Escherichia coli</i> . Here, we investigated the contribution of EmrAB-TolC to metabolism in various conditions. Deletion of EmrB led to changes in several metabolic pathways, both in standard growth conditions and during nutrient stress. The pathways impacted include the tricarboxylic acid (TCA) cycle, and carbohydrate and amino acid metabolism. Our findings suggest that EmrAB-TolC contributes to maintaining metabolic homeostasis and adapts metabolism based on cellular needs.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation on possible resistance of Candida auris to irradiation with UVC (254 nm).
microPublication biology Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001421
Anna-Maria Gierke, Blende Demelezi, Martin Hessling
{"title":"Investigation on possible resistance of <i>Candida auris</i> to irradiation with UVC (254 nm).","authors":"Anna-Maria Gierke, Blende Demelezi, Martin Hessling","doi":"10.17912/micropub.biology.001421","DOIUrl":"10.17912/micropub.biology.001421","url":null,"abstract":"<p><p><i>Candida auris</i> is a yeast that is increasingly pathogenic due to increased multiresistance to antimycotics. UV (ultraviolet) radiation is still capable of reducing <i>C. auris</i> and offers possible applications in radiation disinfection of surfaces and air. However, it is unknown whether <i>C. auris</i> could also develop a resistance against UV radiation. For this reason, the extent to which <i>C. auris</i> can develop resistance to UVC radiation (254 nm) over several irradiation cycles is being examined. The yeast is irradiated with a UVC dose of 18.2 mJ/cm <sup>2</sup> per cycle, followed by a recovery phase under dark conditions. This irradiation-recovery process was repeated for 16 consecutive cycles, ensuring that the UVC dose of 18.2 mJ/cm <sup>2</sup> remained constant throughout all cycles. It was observed that the log reduction (a log 1 reduction corresponds to a 90% reduction) decreased with increasing number of cycles. Resistance development against UVC could be presented.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome characteristics of cluster EA Microbacterium bacteriophages HungryHenry, CaptainRex, and ChikPic isolated from soil in Tennessee.
microPublication biology Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001499
Sergei Markov, Clayton Barnes, Ariel Hensley, Nicholas More
{"title":"Genome characteristics of cluster EA <i>Microbacterium</i> bacteriophages HungryHenry, CaptainRex, and ChikPic isolated from soil in Tennessee.","authors":"Sergei Markov, Clayton Barnes, Ariel Hensley, Nicholas More","doi":"10.17912/micropub.biology.001499","DOIUrl":"10.17912/micropub.biology.001499","url":null,"abstract":"<p><p>Bacteriophages HungryHenry, CaptainRex, and ChikPic were isolated from soil collected in Tennessee using the bacterium <i>Microbacterium foliorum</i> . These bacteriophages have genomes that are 41,516 bp, 39,941 bp, and 40,333 bp in length and encode 62, 61, and 63 putative genes, respectively. Based on gene content similarity, all three bacteriophages are assigned to actinobacteriophage cluster EA (subclusters EA1, EA2 and EA5, respectively).</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A commonly used anti-SOX15 antibody fails to demonstrate specificity in mouse embryos.
microPublication biology Pub Date : 2025-03-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001558
Farina Aziz, Bin Gu, Amy Ralston
{"title":"A commonly used anti-SOX15 antibody fails to demonstrate specificity in mouse embryos.","authors":"Farina Aziz, Bin Gu, Amy Ralston","doi":"10.17912/micropub.biology.001558","DOIUrl":"10.17912/micropub.biology.001558","url":null,"abstract":"<p><p>SOX15 is a broadly conserved transcription factor involved in many critical processes, including mammalian cell fate specification and pluripotency. We investigated the specificity of a commercially available, polyclonal anti-SOX15 antibody advertised as knockout-validated. We generated a new mouse line carrying a null allele of <i>Sox15</i> , and then evaluated anti-SOX15 activity in <i>Sox15</i> null mouse embryos. Nuclear signal was detected in both wild type and null embryos, even when potential maternally or paternally-contributed <i>Sox15</i> was eliminated. We conclude that this SOX15-detecting reagent may not be suitable for all applications, and caution the growing community of users accordingly.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11962655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143774881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone morphogenetic protein 8B (BMP8B) increases the glucose sensitivity of ventromedial hypothalamus (VMH) glucose-inhibited (GI) neurons in female mice.
microPublication biology Pub Date : 2025-03-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001496
Pamela R Hirschberg, Munir Rahbe, Kevin Knapp, Hamad Wajid, Vanessa H Routh, Pallabi Sarkar
{"title":"Bone morphogenetic protein 8B (BMP8B) increases the glucose sensitivity of ventromedial hypothalamus (VMH) glucose-inhibited (GI) neurons in female mice.","authors":"Pamela R Hirschberg, Munir Rahbe, Kevin Knapp, Hamad Wajid, Vanessa H Routh, Pallabi Sarkar","doi":"10.17912/micropub.biology.001496","DOIUrl":"10.17912/micropub.biology.001496","url":null,"abstract":"<p><p>In female, but not male, mice, bone morphogenetic protein (BMP) 8B affects energy homeostasis by inhibiting AMP activated protein kinase (AMPK) in the ventromedial hypothalamus (VMH). VMH glucose-inhibited (GI) neurons that express neuronal nitric oxide synthase (nNOS) increase blood glucose in an AMPK dependent fashion. We tested the hypothesis that BMP8B increases glucose inhibition on VMH nNOS-GI neurons. We found that more VMH nNOS neurons expressed the BMP8B receptor in females than males. Moreover, BMP8B blunted activation of VMH GI neurons in low glucose. Thus, VMH nNOS-GI neurons may mediate some of the metabolic effects of BMP8B in females.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SR Protein Kinase is expressed in Drosophila ovarian germline stem cells but is not essential for their self-renewal.
microPublication biology Pub Date : 2025-03-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001550
Victoria E Garrido, William G Outlaw, Amanda M Powell, Elizabeth T Ables
{"title":"SR Protein Kinase is expressed in <i>Drosophila</i> ovarian germline stem cells but is not essential for their self-renewal.","authors":"Victoria E Garrido, William G Outlaw, Amanda M Powell, Elizabeth T Ables","doi":"10.17912/micropub.biology.001550","DOIUrl":"10.17912/micropub.biology.001550","url":null,"abstract":"<p><p>Germline stem cells (GSCs) are necessary for oocyte production in <i>Drosophila</i> . GSC maintenance is regulated by intrinsic factors that promote their self-renewal. One such factor, the beta-importin, <i>Transportin-Serine/Arginine rich</i> , mediates nuclear import of serine/arginine-rich (SR) proteins, which are phosphorylated by SR protein kinases. Here, we investigate whether the kinase encoded by <i>SR protein kinase</i> ( <i>SRPK</i> ) is essential for GSC self-renewal. We find that SRPK is expressed in GSCs and their mitotically-dividing daughters, but is not necessary for GSC establishment or maintenance. We conclude that SRPK is dispensable for GSC self-renewal, and postulate that other protein kinases can compensate for its absence.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信