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Replication of seizure-suppressing effects of alpha-linolenic acid on the Drosophila melanogaster para Shudderer mutant. α -亚麻酸对黑腹果蝇颤振突变体癫痫抑制作用的复制。
microPublication biology Pub Date : 2025-09-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001815
Samantha Dinnel, Brady Weimer, Allyson Davis, Katie Gerovac, Leah M Hawbaker, Matthew Maser, Meghan Shannon, Douglas J Brusich
{"title":"Replication of seizure-suppressing effects of alpha-linolenic acid on the <i>Drosophila melanogaster para <sup>Shudderer</sup></i> mutant.","authors":"Samantha Dinnel, Brady Weimer, Allyson Davis, Katie Gerovac, Leah M Hawbaker, Matthew Maser, Meghan Shannon, Douglas J Brusich","doi":"10.17912/micropub.biology.001815","DOIUrl":"https://doi.org/10.17912/micropub.biology.001815","url":null,"abstract":"<p><p>Voltage-gated sodium channels are essential for healthy nervous system function. Mutations in voltage-gated sodium channels are associated with a range of seizure conditions. The genetics of seizure conditions are complex and often challenging to study or replicate in animal models. The <i>Drosophila melanogaster</i> gene <i>paralytic</i> ( <i>para</i> ) is the sole voltage-gated sodium channel gene in flies. The <i>para <sup>Shudderer</sup></i> allele causes dominant seizure activity manifest in adult morphology and behavior. In this study we replicated previous findings of <i>para <sup>Shudderer</sup></i> hyperexcitability and the ability to suppress this activity with dietary supplementation of an omega-3 fatty acid, alpha-linolenic acid (ALA). Our results support the robustness of the <i>para <sup>Shudderer</sup></i> phenotype and the replicability of findings across separate lab environments.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145253831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The two coiled-coil domains of the fission yeast kinesin-6 Klp9 are required for motor tetramerization and spindle elongation. 裂变酵母激酶-6 Klp9的两个盘绕结构域是电机四聚化和纺锤体伸长所必需的。
microPublication biology Pub Date : 2025-09-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001829
Mason T Nguyen, Liang Ji, Phong T Tran
{"title":"The two coiled-coil domains of the fission yeast kinesin-6 Klp9 are required for motor tetramerization and spindle elongation.","authors":"Mason T Nguyen, Liang Ji, Phong T Tran","doi":"10.17912/micropub.biology.001829","DOIUrl":"https://doi.org/10.17912/micropub.biology.001829","url":null,"abstract":"<p><p>The fission yeast kinesin-6 Klp9 localizes to the spindle midzone at anaphase to produce sliding forces to elongate the bipolar spindle. In the absence of Klp9 , anaphase spindle elongation is attenuated by half its normal rate. Klp9 functions as a microtubule plus end-directed tetrameric motor. Tetramerization is the key to its microtubule sliding function, as tetramerization allows Klp9 to bind antiparallel microtubules at the midzone. The amino acid sequence of Klp9 indicates two alpha-helical coiled-coils domains CC1 and CC2, important for protein-protein interactions. We seeked the potential oligomerization states of Klp9 via its coiled-coils using AlphaFold3. AlphaFold predicted that CC1 can form dimers and together with CC2 can form tetramers. The different oligomeric states enabled precise experimental verifications. We measured Klp9 motor GFP intensity and anaphase spindle elongation rate for the full-length Klp9 , Klp9-deletion (Klp9Δ), and truncated Klp9 containing no coiled-coils, or only CC1, or both CC1 and CC2. The results indicate that: 1) GFP intensity increases with increasing oligomeric state, and 2) attenuated anaphase spindle velocity is restored only in the Klp9 truncation containing both CC1 and CC2. The experimental data are consistent with prediction, indicating that CC1 contributes to Klp9 dimerization, and that CC1 and CC2 together contribute to Klp9 tetramerization.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145253888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exclusion of the endoplasmic reticulum from the C. elegans meiotic spindle controls spindle size. 从秀丽隐杆线虫减数分裂纺锤体中排除内质网控制纺锤体大小。
microPublication biology Pub Date : 2025-09-19 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001812
Alma Aquino, Francis McNally
{"title":"Exclusion of the endoplasmic reticulum from the <i>C. elegans</i> meiotic spindle controls spindle size.","authors":"Alma Aquino, Francis McNally","doi":"10.17912/micropub.biology.001812","DOIUrl":"10.17912/micropub.biology.001812","url":null,"abstract":"<p><p>The nuclear envelope is composed of sheet-like ER that separates chromatin from tubulin during interphase. During oocyte maturation in <i>C. elegans</i> , a fenestrated envelope of sheet-like ER continues to envelope the meiotic spindle through metaphase I. ER is thus excluded from the nuclear/spindle volume during spindle assembly. To test the importance of this exclusion, we forced ER into the meiotic spindle by coupling kinesin motor domains to the ER. Forcing ER into the spindle interior caused a statistically significant increase in metaphase spindle width. Exclusion of ER from the spindle thus affects spindle geometry.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12495482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cells stably expressing shRNA against MYO10 display altered cell motility. 稳定表达shRNA对抗MYO10的细胞表现出改变的细胞运动性。
microPublication biology Pub Date : 2025-09-19 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001764
Joanna A Mas, Chase E Cristella, Vu Hao M N Phan, Lillian S Wendt, Charlotte A Rose, Abigail Ali, David F Carpio, Christine Cole, Paige Embley, Jack E Hoskins-Harris, Delia Johnson, Noelle Ledoux, Hannah W Lwin, Sarah Salah, Erin Weisbart, Stacey J Criswell, Omar Alberto Quintero-Carmona
{"title":"Cells stably expressing shRNA against MYO10 display altered cell motility.","authors":"Joanna A Mas, Chase E Cristella, Vu Hao M N Phan, Lillian S Wendt, Charlotte A Rose, Abigail Ali, David F Carpio, Christine Cole, Paige Embley, Jack E Hoskins-Harris, Delia Johnson, Noelle Ledoux, Hannah W Lwin, Sarah Salah, Erin Weisbart, Stacey J Criswell, Omar Alberto Quintero-Carmona","doi":"10.17912/micropub.biology.001764","DOIUrl":"10.17912/micropub.biology.001764","url":null,"abstract":"<p><p>Myosin-X (MYO10) is an actin-based motor protein involved in cytoskeletal dynamics, membrane interactions, and integrin-mediated adhesion. To investigate MYO10's cellular roles, we generated MYO10 knockdown (MYO10 <sup>KD</sup> ) HeLa and COS7 cell lines using lentiviral shRNA. Compared to wild-type cells, both MYO10 <sup>KD</sup> lines showed reduced proliferation and impaired cell migration in wound assays. Additionally, there were fewer edge filopodia in HeLa cells. Furthermore, MYO10 <sup>KD</sup> cells demonstrated increased spreading on laminin-coated substrates, suggesting altered integrin activation and cytoskeletal linkage. Our results reinforce MYO10's importance in cell proliferation, adhesion, and migration. These MYO10 <sup>KD</sup> lines provide an accessible cell culture model for future study of MYO10.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12495483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aberrant expression of MAPK1 and MCTS1 in chronic myeloid leukemia (CML). MAPK1和MCTS1在慢性髓性白血病（CML）中的异常表达。
microPublication biology Pub Date : 2025-09-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001681
Leo Kortendick, Corinna Meyer, Stefan Nagel
{"title":"Aberrant expression of MAPK1 and MCTS1 in chronic myeloid leukemia (CML).","authors":"Leo Kortendick, Corinna Meyer, Stefan Nagel","doi":"10.17912/micropub.biology.001681","DOIUrl":"10.17912/micropub.biology.001681","url":null,"abstract":"<p><p>Genomic amplification may result in aberrant gene expression and support development of cancer, including chronic myeloid leukemia (CML). In CML cell line K-562, we recently reported overexpression of TBX1 located at chromosomal position 22q11, focally co-amplified together with BCR, part of the CML hallmark fusion gene BCR::ABL1. Here, we extended that study, by identifying genomically amplified and overexpressed MAPK1/ERK2 at 22q11 together with MCTS1 at Xq22. Using pharmacological inhibitors and siRNA-mediated knockdown assays, our data collectively revealed novel regulatory connections between TBX1, MAPK1 and MCTS1, which may play a role in drug resistance.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The chromosome complement of male Chrysoperla rufilabris (Burmeister, 1839), with evidence of distance segregation in meiosis I. 雄性蝶蛹（Chrysoperla rufilabris）的染色体补体（Burmeister, 1839），与减数分裂I中距离分离的证据。
microPublication biology Pub Date : 2025-09-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001770
Valoria Maslin, Allison Eisenhauer, Leocadia Paliulis
{"title":"The chromosome complement of male <i>Chrysoperla rufilabris</i> (Burmeister, 1839), with evidence of distance segregation in meiosis I.","authors":"Valoria Maslin, Allison Eisenhauer, Leocadia Paliulis","doi":"10.17912/micropub.biology.001770","DOIUrl":"10.17912/micropub.biology.001770","url":null,"abstract":"<p><p>Neuropteran insects display a phenomenon called distance segregation during male meiosis I, where the X and Y chromosomes are unconnected from prometaphase I through anaphase I. Here we investigate chromosome number and patterns of chromosome segregation of a previously unstudied Neuropteran: the green lacewing, <i>Chrysoperla rufilabris</i> (Burmeister, 1839). Testes of male <i>C. rufilabris</i> were isolated and prepared following a chromosome squash method. Living meiosis I spermatocytes were also imaged. The data revealed a chromosome number of 2n=12 in males (2n=10+XY) and evidence of distance segregation of X and Y chromosomes in meiosis I spermatocytes.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Starvation-Induced Disruption of Nocturnal activity in the tropical house cricket, Gryllodes sigillatus. 饥饿引起的热带蟋蟀夜间活动中断。
microPublication biology Pub Date : 2025-09-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001701
Hugo Moebel, Eshani Yeragi, Isaac Jones, Alex Keene
{"title":"Starvation-Induced Disruption of Nocturnal activity in the tropical house cricket, <i>Gryllodes sigillatus</i>.","authors":"Hugo Moebel, Eshani Yeragi, Isaac Jones, Alex Keene","doi":"10.17912/micropub.biology.001701","DOIUrl":"10.17912/micropub.biology.001701","url":null,"abstract":"<p><p>Feeding state potently modulates foraging behavior and locomotor activity. Here, we examined activity patterns across developmental stages in the nocturnal tropical house cricket or banded cricket, <i>Gryllodes sigillatus</i> . Adult males and females exhibited robust nocturnal behavior, with nocturnality emerging earlier in females during development. Starvation significantly reduced overall activity and abolished nocturnal activity patterns in females, but not in males. These results reveal sex-dependent differences in the developmental and feeding-state regulation of nocturnal behavior in <i>G. sigillatus</i> and lay the groundwork for future studies on how nutrient stress modulates behavior in this species.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Validating and Optimizing a Drosophila Larval Model of Parkinson's Synucleopathy. 帕金森突触病果蝇幼虫模型的验证和优化。
microPublication biology Pub Date : 2025-09-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001592
Sarah Perry, Nicholas More
{"title":"Validating and Optimizing a <i>Drosophila</i> Larval Model of Parkinson's Synucleopathy.","authors":"Sarah Perry, Nicholas More","doi":"10.17912/micropub.biology.001592","DOIUrl":"10.17912/micropub.biology.001592","url":null,"abstract":"<p><p>Parkinson's disease (PD) is a prevalent neurodegenerative disorder with complex genetic and environmental underpinnings. Misexpression of human alpha-synuclein (αSyn) in <i>Drosophila</i> larval dopaminergic neurons has been shown to induce PD-like phenotypes, yet this model has not been fully optimized to assess different αSyn variants and their effects on various neuronal subtypes. By expanding the use of the <i>Drosophila</i> larval model, we demonstrate that dopaminergic neurons exhibit pronounced vulnerability to αSyn, particularly to E46K and A53T variants. Additionally, we show that vitamin C effectively mitigates locomotor impairments, highlighting oxidative stress as a key contributor to αSyn-induced dysfunction.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ogremorphin inhibits GPR68 mediated MUC5AC expression. Ogremorphin抑制GPR68介导的MUC5AC表达。
microPublication biology Pub Date : 2025-09-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001639
Leif R Neitzel, Hussain F Basrai, Charles C Hong, Charles H Williams
{"title":"Ogremorphin inhibits GPR68 mediated MUC5AC expression.","authors":"Leif R Neitzel, Hussain F Basrai, Charles C Hong, Charles H Williams","doi":"10.17912/micropub.biology.001639","DOIUrl":"10.17912/micropub.biology.001639","url":null,"abstract":"<p><p>MUC5AC hypersecretion exacerbates airway obstruction in lung diseases, such as asthma, and promotes tumor growth. The expression of MUC5AC is driven by the pH-sensing receptor, GPR68. Herein we demonstrate that acidification (pH 6.4) increased MUC5AC mRNA and protein expression in a GPR68-dependent manner in A549 cells. Treatment with Ogremorphin (OGM), a specific GPR68 inhibitor, reduced MUC5AC mRNA and protein expression irrespective of pH. These findings were confirmed by shRNA-mediated knockdown of GPR68. These data suggest GPR68 inhibition by OGM as a potential therapy for mucus hypersecretion to improve airway clearance and reduce tumor progression in lung diseases.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reduced insulin-like signaling restores motor and chemosensory neuron function in C. elegans expressing TDP-43, an ALS-associated protein. 减少胰岛素样信号可以恢复秀丽隐杆线虫表达TDP-43（一种als相关蛋白）的运动和化学感觉神经元功能。
microPublication biology Pub Date : 2025-09-16 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001788
Vincent Lotesto, Cindy Voisine
{"title":"Reduced insulin-like signaling restores motor and chemosensory neuron function in <i>C. elegans</i> expressing TDP-43, an ALS-associated protein.","authors":"Vincent Lotesto, Cindy Voisine","doi":"10.17912/micropub.biology.001788","DOIUrl":"10.17912/micropub.biology.001788","url":null,"abstract":"<p><p>TAR DNA-binding Protein 43 (TDP-43) is linked to the pathology of neurodegenerative diseases. We used the roundworm <i>Caenorhabditis elegans</i> to examine the neurotoxic impact of the pan-neuronal expression of wild-type human TDP-43 (hTDP-43) fused to a yellow fluorescent protein. Using the <i>daf-2 ( e1370 )</i> mutant allele, we sought to determine whether activating cellular stress responses in the insulin-like signaling (ILS) pathway could restore neuronal function in hTDP-43 expressing <i>C. elegans</i> . Using well characterized behavioral assays, our data show that manipulating the ILS pathway significantly improves functionality of motor and chemosensory neurons in animals expressing hTDP-43.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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