发布求助
文献互助 智能选刊 最新文献

microPublication biology最新文献

筛选
英文 中文
MEMO1-ERα interaction may occur independent of Y537 phosphorylation which differs from MEMO1-ErbB2 binding. MEMO1-ERα相互作用可能独立于Y537磷酸化发生，这与MEMO1-ErbB2结合不同。
microPublication biology Pub Date : 2026-09-29 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002363
Paxton Mills, Haley Salus, Khanh Vu, Lauren Shin, Catherine A Bayas, Joshua Pandian, Carol A Parish, Julie A Pollock
{"title":"MEMO1-ERα interaction may occur independent of Y537 phosphorylation which differs from MEMO1-ErbB2 binding.","authors":"Paxton Mills, Haley Salus, Khanh Vu, Lauren Shin, Catherine A Bayas, Joshua Pandian, Carol A Parish, Julie A Pollock","doi":"10.17912/micropub.biology.002363","DOIUrl":"10.17912/micropub.biology.002363","url":null,"abstract":"<p><p>MEMO1 is a protein implicated in cancer-associated signaling through interactions with other proteins, including estrogen receptor α (ERα). We used fluorescence polarization, molecular dynamics simulations, and protein pull-down assays to characterize the interaction between MEMO1 and ERα. MEMO1 bound to an ERα peptide containing phosphorylated Y537 but unexpectedly showed comparable affinity for the non-phosphorylated peptide. Computational modeling identified distinct binding modes and suggested contributions from MEMO1 residues. Pull-down experiments confirmed interaction between MEMO1 and the ERα ligand-binding domain. These findings suggest that MEMO1 interacts with ERα in a phosphorylation-independent manner, highlighting diverse binding modes that may support its scaffolding functions.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The limit of detection of a Phytophthora agathidicida LAMP assay is higher under alternative laboratory conditions. 在不同的实验室条件下，一种抗疫疫霉菌LAMP法的检出限较高。
microPublication biology Pub Date : 2026-09-29 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002163
Jade Tt Palmer, Monica L Gerth
{"title":"The limit of detection of a <i>Phytophthora agathidicida</i> LAMP assay is higher under alternative laboratory conditions.","authors":"Jade Tt Palmer, Monica L Gerth","doi":"10.17912/micropub.biology.002163","DOIUrl":"https://doi.org/10.17912/micropub.biology.002163","url":null,"abstract":"<p><p>Surveillance of <i>Phytophthora agathidicida</i> , the causal agent of kauri dieback, has included soil baiting followed by loop-mediated isothermal amplification (LAMP)-based detection. This approved assay was reported to detect 1 fg of DNA using OptiGene Isothermal Master Mix on a BioRanger instrument. We evaluated its analytical robustness using the same master mix on an alternative platform (QuantStudio 3), and separately tested an alternative NEB master mix. Both assays yielded a reliable detection limit of 1,000 fg, with variable detection at 100 fg and none below. Diagnostic laboratories should verify assay performance under their operational conditions prior to implementation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome Analysis of Two Arthrobacter Bacteriophages, Andrew and RedFox, from Subcluster AS3 Isolated from Soil in New York and Pennsylvania. 纽约和宾夕法尼亚土壤AS3亚群中两个节肢细菌噬菌体Andrew和RedFox的基因组分析
microPublication biology Pub Date : 2026-09-29 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002367
Paige Baldwin, Megan Dennis, Niran Isik, Hanan Isik, Deborah Jacobs-Sera, Juliana Magna, Mia Mazakas, Gabriella Rice, Sarah Swerdlow, Megan Ulbrich, Matthew Farber, Marina Bogush
{"title":"Genome Analysis of Two <i>Arthrobacter</i> Bacteriophages, Andrew and RedFox, from Subcluster AS3 Isolated from Soil in New York and Pennsylvania.","authors":"Paige Baldwin, Megan Dennis, Niran Isik, Hanan Isik, Deborah Jacobs-Sera, Juliana Magna, Mia Mazakas, Gabriella Rice, Sarah Swerdlow, Megan Ulbrich, Matthew Farber, Marina Bogush","doi":"10.17912/micropub.biology.002367","DOIUrl":"https://doi.org/10.17912/micropub.biology.002367","url":null,"abstract":"<p><p>Novel bacteriophages Andrew and RedFox were isolated from soil samples collected in New York and Pennsylvania and propagated using <i>Arthrobacter globiformis</i> B-2979. Both phages are assigned to actinobacteriophage subcluster AS3 based on gene content. Although these phages exhibit conserved genomic content characteristics of the AS3 subcluster, there are notable differences. In particular, phage Andrew gene <i>25</i> in the central region of the genome and genes <i>64, 65,</i> and <i>66</i> near the end of the genome lack homologs in cluster AS3 or in the actinobacteriophage database more broadly.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13679992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149229128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of two Mutator insertion alleles of Chr101 during maize vegetative development. 玉米Chr101两个突变插入等位基因在营养发育中的特性分析。
microPublication biology Pub Date : 2026-09-29 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002431
Alexia Gulino, Gursimer Anand, Mya Goodson, Navjot Nijjar, Ketzia Obi, Maria Salazar, Dafang Wang
{"title":"Characterization of two Mutator insertion alleles of <i>Chr101</i> during maize vegetative development.","authors":"Alexia Gulino, Gursimer Anand, Mya Goodson, Navjot Nijjar, Ketzia Obi, Maria Salazar, Dafang Wang","doi":"10.17912/micropub.biology.002431","DOIUrl":"10.17912/micropub.biology.002431","url":null,"abstract":"<p><p>CHR101 ( <i>ZmDDM1A</i> ) encodes a chromatin remodeling factor involved in DNA methylation maintenance and transposable element silencing in maize. We analyzed two <i>Mutator (Mu)</i> insertion alleles of <i>Chr101</i> : <i>chr101-m1</i> , containing an insertion in the 5' untranslated region, and <i>chr101-m3</i> , containing an insertion in exon 3. Quantitative RT-PCR revealed that <i>Chr101</i> transcript abundance was reduced by approximately 76-93% in both homozygous mutant lines at juvenile (V4) and adult (V7) vegetative stages. Reduced expression of <i>Aspartic Proteinase A1</i> ( <i>Apa1</i> ) was also observed in the mutant backgrounds examined.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149217635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum: Erratum: Pubertal development is unaffected in female Mecp2 -heterozygous mice. 雌性Mecp2杂合小鼠的青春期发育不受影响。
microPublication biology Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002442
{"title":"Erratum: Erratum: Pubertal development is unaffected in female <i>Mecp2</i> -heterozygous mice.","authors":"","doi":"10.17912/micropub.biology.002442","DOIUrl":"https://doi.org/10.17912/micropub.biology.002442","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.17912/micropub.biology.002400.].</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete Genome Sequences of Bacteriophages Pulchra and Vanisius Isolated on Microbacterium foliorum . 叶状微杆菌分离的Pulchra和Vanisius噬菌体全基因组序列。
microPublication biology Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.001860
Elvira R Eivazova, Gregory S Markov, Tessa C Cote Allen, Ava E Brittain, Jenna St Pierre, Madalyn N Ellis, Michael J Pollack
{"title":"Complete Genome Sequences of Bacteriophages Pulchra and Vanisius Isolated on <i>Microbacterium foliorum</i> .","authors":"Elvira R Eivazova, Gregory S Markov, Tessa C Cote Allen, Ava E Brittain, Jenna St Pierre, Madalyn N Ellis, Michael J Pollack","doi":"10.17912/micropub.biology.001860","DOIUrl":"https://doi.org/10.17912/micropub.biology.001860","url":null,"abstract":"<p><p>Actinobacteriophages Pulchra and Vanisius were isolated from soil samples using <i>Microbacterium foliorum</i> NRRL B-24224. Pulchra and Vanisius have genomes of 53312 bp and 17453bp encoding 91 and 25 predicted protein-coding genes, respectively. Both phages exhibit a siphovirus morphology and have the same GC content of 68.8%. Based on gene content, Pulchra and Vanisius are assigned to actinobacteriophage clusters EC and EE, correspondingly.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete Genome Sequence of Arthrobacter globiformis Bacteriophage Ren19. 球形节杆菌噬菌体Ren19全基因组序列。
microPublication biology Pub Date : 2026-09-28 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002350
Lauren A Holt, Jazmine N Taylor, Kera S Ridley, Devin-Danielle V Webb, David S I Davenport, He Fu
{"title":"Complete Genome Sequence of <i>Arthrobacter globiformis</i> Bacteriophage Ren19.","authors":"Lauren A Holt, Jazmine N Taylor, Kera S Ridley, Devin-Danielle V Webb, David S I Davenport, He Fu","doi":"10.17912/micropub.biology.002350","DOIUrl":"https://doi.org/10.17912/micropub.biology.002350","url":null,"abstract":"<p><p>Bacteriophage Ren19, isolated from a soil sample collected in Greensboro, North Carolina, USA, exhibits a siphovirus morphology and infects <i>Arthrobacter globiformis</i> B-2979. Its 68,603-bp genome has a GC content of 65.4% and contains 118 predicted genes. Based on gene-content similarity, Ren19 is assigned to actinobacteriophage cluster AP2.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome and Characteristics of Bacteriophages Stardrop and Laphuphu24k. 噬菌体Stardrop和Laphuphu24k的基因组和特性。
microPublication biology Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002329
Rylee G Oberbeck, Oliver F Jansson, Shravani Khadilkar, Trey H Pyle, Lucas A Rossi, Sophia R Salazar, J Trevor O'Neill, Stephanie L Mathews
{"title":"Genome and Characteristics of Bacteriophages Stardrop and Laphuphu24k.","authors":"Rylee G Oberbeck, Oliver F Jansson, Shravani Khadilkar, Trey H Pyle, Lucas A Rossi, Sophia R Salazar, J Trevor O'Neill, Stephanie L Mathews","doi":"10.17912/micropub.biology.002329","DOIUrl":"https://doi.org/10.17912/micropub.biology.002329","url":null,"abstract":"<p><p>Bacteriophages (phages) are viruses that infect bacteria and are the most abundant biological entity on Earth. Two novel phages: Stardrop and Laphuphu24k, capable of infecting <i>Arthrobacter globiformis</i> B-2979, were isolated from soil in Raleigh, North Carolina. Both phages have siphovirus morphology as examined by transmission electron microscopy. Stardrop is a lytic AU6 cluster actinobacteriophage with 94 genes and Laphuphu24k is a lysogenic AS3 cluster actinobacteriophage with 68 genes.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13653268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Validation of CRISPRoff guide RNAs for stable repression of human AKT1. CRISPRoff引导rna稳定抑制人类AKT1的验证。
microPublication biology Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002320
Yudai Takahashi, Hidenori Matsuzaki
{"title":"Validation of CRISPRoff guide RNAs for stable repression of human AKT1.","authors":"Yudai Takahashi, Hidenori Matsuzaki","doi":"10.17912/micropub.biology.002320","DOIUrl":"https://doi.org/10.17912/micropub.biology.002320","url":null,"abstract":"<p><p>CRISPRoff enables stable gene repression through targeted DNA methylation, but its efficiency depends on guide RNA selection. To identify effective guide RNAs for human AKT1, we evaluated ten guide RNAs targeting the AKT1 promoter region in HEK293 cells. Guide RNA g1 produced the strongest reduction in Akt1 protein expression, whereas g4, g5, g7, and g9 also reproducibly reduced Akt1 levels. Interestingly, closely spaced or partially overlapping guide RNAs exhibited markedly different silencing efficiencies. These validated guide RNAs provide a useful resource for future studies of Akt signaling.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13653269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The efficacy of calcein as a tagging agent for the long-spined sea urchin, Diadema antillarum. 钙黄蛋白对长刺海胆的标记作用。
microPublication biology Pub Date : 2026-09-25 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002242
Benjamin A Belgrad, Lindsay J Spiers, William C Sharp
{"title":"The efficacy of calcein as a tagging agent for the long-spined sea urchin, <i>Diadema antillarum</i>.","authors":"Benjamin A Belgrad, Lindsay J Spiers, William C Sharp","doi":"10.17912/micropub.biology.002242","DOIUrl":"https://doi.org/10.17912/micropub.biology.002242","url":null,"abstract":"<p><p>The sea urchin <i>Diadema antillarum</i> is a restoration target due to its function as a keystone reef herbivore. However, restocking efforts are hindered by a lack of long-term tagging methods. We tested the effectiveness of calcein marking for urchin releases. Cultured individuals were tagged using a 150 mg/L calcein bath and monitored for 11 weeks. Dye markings remained fully visible in all individuals for two weeks but began to fade within three weeks. Only individuals with white spines maintained markings beyond five weeks. Unlike with other urchin species, calcein only seems suitable for short-term external tagging of <i>D. antillarum</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13653267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书