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Isolation and Annotation of Arthrobacter globiformis Phage AWGoat.
microPublication biology Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001433
Caitlyn Bolling, Chloe Chervenic, Kate Drake, Zoe Griffin, Isha Jain, Hala Khabir, Tyler Ordon, Anthony Padilla, Rachel Showers, Jose Vences, Caelan Walsh, Wyatt Workman, Sharon Bullock, Michelle Pass, Tonya Bates, Ellen Wisner
{"title":"Isolation and Annotation of <i>Arthrobacter globiformis</i> Phage AWGoat.","authors":"Caitlyn Bolling, Chloe Chervenic, Kate Drake, Zoe Griffin, Isha Jain, Hala Khabir, Tyler Ordon, Anthony Padilla, Rachel Showers, Jose Vences, Caelan Walsh, Wyatt Workman, Sharon Bullock, Michelle Pass, Tonya Bates, Ellen Wisner","doi":"10.17912/micropub.biology.001433","DOIUrl":"10.17912/micropub.biology.001433","url":null,"abstract":"<p><p>AWGoat, a lytic bacteriophage that infects <i>Arthrobacter globiformis,</i> was isolated from a goat pen in Charlotte, NC. Its genome is 65,496 bp long, with a GC content of 67.1%. Based on gene content similarity, AWGoat is assigned to actinobacteriophage cluster AP. It encodes a putative toxin from a broadly distributed toxin/antitoxin pair and an unusually large minor tail protein.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete Genome Sequences of Two Arthrobacter Phages, assigned to clusters AT and AZ.
microPublication biology Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001417
Sarah J Swerdlow, Melinda Harrison
{"title":"Complete Genome Sequences of Two Arthrobacter Phages, assigned to clusters AT and AZ.","authors":"Sarah J Swerdlow, Melinda Harrison","doi":"10.17912/micropub.biology.001417","DOIUrl":"10.17912/micropub.biology.001417","url":null,"abstract":"<p><p>Two bacteriophages, BeatusComedenti and Cyan, were isolated using <i>Arthrobacter sp.</i> and <i>Arthrobacter globiformis</i> , respectively. BeatusComedenti and Cyan, which contain 100 and 70 putative genes, are assigned to actinobacteriophage clusters AT and AZ1, respectively.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete Genome Sequences of Mycobacterium smegmatis Phages Dove and Issimir.
microPublication biology Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001432
Victoria Frost, Israel Bellinger, Riley Burn, Chastity Chisholm, Fisher Cobb, Hannah Duncan, Kalli Green, Madelynn Harding, Amari Johnson, Rachel Leek, Breanna Menard, Ciaran Murphy, Destiny Thompson, Gwendolyn Tomlin, Kristi Westover
{"title":"Complete Genome Sequences of <i>Mycobacterium smegmatis</i> Phages Dove and Issimir.","authors":"Victoria Frost, Israel Bellinger, Riley Burn, Chastity Chisholm, Fisher Cobb, Hannah Duncan, Kalli Green, Madelynn Harding, Amari Johnson, Rachel Leek, Breanna Menard, Ciaran Murphy, Destiny Thompson, Gwendolyn Tomlin, Kristi Westover","doi":"10.17912/micropub.biology.001432","DOIUrl":"10.17912/micropub.biology.001432","url":null,"abstract":"<p><p>We announce the discovery of two mycobacteriophages isolated from soil in Rock Hill, South Carolina. Phage Dove has a genome sequence length of 108,976bp, a siphovirus morphology, and a predicted temperate lifecycle. Phage Issimir has a genome sequence length of 155,564bp, a myovirus morphology, and a predicted lytic lifecycle.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fecundities of Hawaiian Caenorhabditis briggsae wild strains are not correlated with natural niche temperatures.
microPublication biology Pub Date : 2025-01-15 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001356
Nikita S Jhaveri, Erik C Andersen
{"title":"Fecundities of Hawaiian <i>Caenorhabditis briggsae</i> wild strains are not correlated with natural niche temperatures.","authors":"Nikita S Jhaveri, Erik C Andersen","doi":"10.17912/micropub.biology.001356","DOIUrl":"10.17912/micropub.biology.001356","url":null,"abstract":"<p><p>Adaptation to the local environment is crucial for organisms to survive and reproduce in the natural niche. The nematode <i>Caenorhabditis briggsae</i> has been studied extensively and hypothesized to be adapted to temperatures at the geographic sites where nematodes were isolated from nature. We empirically tested if this correlation is biologically meaningful by measuring the fitness of <i>C. briggsae</i> wild strains isolated from different temperatures across the Hawaiian islands. We did not observe a correlation between lifetime fecundity and the temperature of the natural niche from where the strains were isolated, indicating that this fitness trait might not be adapted to local environmental temperatures.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quick Oil Red O, Paint3D and Biopython as an economical tool to measure total lipid levels in Caenorhabditis elegans.
microPublication biology Pub Date : 2025-01-14 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001320
Wenqing Wang, Elizabeth R Wong, Michael L Cato, Patrick C Daly, Chandler G Doody, Townshend Gillespie, Caroline N Ksor, Arooj Zainab, Zunaira Zainab, Matt Crook
{"title":"Quick Oil Red O, Paint3D and Biopython as an economical tool to measure total lipid levels in <i>Caenorhabditis elegans</i>.","authors":"Wenqing Wang, Elizabeth R Wong, Michael L Cato, Patrick C Daly, Chandler G Doody, Townshend Gillespie, Caroline N Ksor, Arooj Zainab, Zunaira Zainab, Matt Crook","doi":"10.17912/micropub.biology.001320","DOIUrl":"10.17912/micropub.biology.001320","url":null,"abstract":"<p><p>Quick Oil Red O staining is a well established method to assay total lipid levels in <i>Caenorhabditis elegans</i> , but the software to clean up and analyse the images is either laborious, expensive or both. We have developed a process that uses an existing protocol to stain the animals, followed by Magic Select in Paint3D to remove background and then a custom script in Biopython to quantify average pixel intensity <i>per</i> animal. The software is free, accessible and relatively easy to use for undergraduate researchers.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The AWC OFF neuron is important for attraction to 1-butanol in Caenorhabditis elegans.
microPublication biology Pub Date : 2025-01-14 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001370
Vaughn E Brown, Ella Bradley, Tymmaa A Asaed, Sokhna B Lo, Zach S Bellini, Dylan J Blackett, Jeremy J Callaway, Jacob Hallesy, Zoey E Joshlin, Taryn L Kaneko, Catie H Kaneshiro, Kae R Kidd, Jacinda Lee, Kaitlyn M Leung, Janelle S Li, Ben P Luo, Charlene C Mbaeri, Alanna O'Neill, Precious Omomofe, James D Schmidt, Minh Truong, Elizabeth E Glater
{"title":"The AWC <sup>OFF</sup> neuron is important for attraction to 1-butanol in <i>Caenorhabditis elegans</i>.","authors":"Vaughn E Brown, Ella Bradley, Tymmaa A Asaed, Sokhna B Lo, Zach S Bellini, Dylan J Blackett, Jeremy J Callaway, Jacob Hallesy, Zoey E Joshlin, Taryn L Kaneko, Catie H Kaneshiro, Kae R Kidd, Jacinda Lee, Kaitlyn M Leung, Janelle S Li, Ben P Luo, Charlene C Mbaeri, Alanna O'Neill, Precious Omomofe, James D Schmidt, Minh Truong, Elizabeth E Glater","doi":"10.17912/micropub.biology.001370","DOIUrl":"10.17912/micropub.biology.001370","url":null,"abstract":"<p><p><i>C. elegans</i> uses chemosensation to recognize a variety of odors, many of which are released by bacteria, the major food source of <i>C. elegans</i> . Specific amphid sensory neurons are known to detect different odorants. Here we show that the AWC <sup>OFF</sup> neuron detects the attractive odorant 1-butanol. Because few odorants that are specifically recognized by the AWC <sup>OFF</sup> neuron have been identified, we hope that the identification of this additional odorant will facilitate studies of the role of the AWC <sup>OFF</sup> neuron in odor detection and discrimination.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Orsay virus infection rate declines with age in C. elegans. 奥赛病毒感染率随 elegans 年龄增长而下降
microPublication biology Pub Date : 2025-01-12 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001434
Shivaani Tishadas, Kentaro Noma
{"title":"Orsay virus infection rate declines with age in <i>C. elegans</i>.","authors":"Shivaani Tishadas, Kentaro Noma","doi":"10.17912/micropub.biology.001434","DOIUrl":"10.17912/micropub.biology.001434","url":null,"abstract":"<p><p>The intracellular pathogen response is regulated by multiple <i>pals</i> genes in <i>C. elegans</i> . How such responses change with age is largely unknown. Thus, we investigated potential age-dependent changes in the immune response to the <i>C. elegans</i> -specific Orsay virus . When animals were exposed to equal viral concentrations, the expression of known immune-response <i>pals</i> genes and viral RNAs was lower in aged populations than in young adults. However, when young and aged populations were infected with equal viral loads, <i>pals</i> gene expression did not change with age. Therefore, aged <i>C. elegans</i> experience a decline in viral infection rate.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11764689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perception of Enterococcus faecalis without infection induces fmo-2 in C. elegans. 未感染粪肠球菌会诱导 elegans 中的 fmo-2。
microPublication biology Pub Date : 2025-01-10 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001422
Megan L Schaller, Madeline M Sykes, Sarah A Easow, Faith R Carranza, Angela M Tuckowski, Yatrik M Shah, Scott F Leiser
{"title":"Perception of <i>Enterococcus faecalis</i> without infection induces <i>fmo-2</i> in <i>C. elegans</i>.","authors":"Megan L Schaller, Madeline M Sykes, Sarah A Easow, Faith R Carranza, Angela M Tuckowski, Yatrik M Shah, Scott F Leiser","doi":"10.17912/micropub.biology.001422","DOIUrl":"10.17912/micropub.biology.001422","url":null,"abstract":"<p><p><i>C. elegans</i> pathogenic susceptibility is influenced by the worm's detection of its environment and its capacity to resist and resolve damage following infection. Here, we use a model where worms can sense, but not ingest, the pathogen <i>Enterococcus faecalis</i> (EF) <i>.</i> We identify that perception of EF without infection induces the stress-response gene <i>fmo-2. We</i> further identify that neural and intestinal signaling genes are necessary for <i>fmo-2</i> induction without active infection. Finally, we show that <i>fmo-2</i> overexpression is sufficient to extend lifespan with EF exposure, while <i>fmo-2</i> KO is not detrimental, suggesting that additional <i>fmo-2</i> expression benefits worms in this condition.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alternative mating pattern in Enoploteuthis chunii is associated with polyandry and male-biased sex ratio.
microPublication biology Pub Date : 2025-01-08 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001424
Yuu Moriwaki, Satoshi Kusama, Anri Yamane, Md Nur E Alam, Noriyosi Sato, Noritaka Hirohashi
{"title":"Alternative mating pattern in <i>Enoploteuthis chunii</i> is associated with polyandry and male-biased sex ratio.","authors":"Yuu Moriwaki, Satoshi Kusama, Anri Yamane, Md Nur E Alam, Noriyosi Sato, Noritaka Hirohashi","doi":"10.17912/micropub.biology.001424","DOIUrl":"10.17912/micropub.biology.001424","url":null,"abstract":"<p><p>Although cephalopods are primarily polyandrous, genetic evidence revealed rare monogamy in <i>Watasenia scintillans</i> . Here, we studied the sister species <i>Enoploteuthis chunii</i> . We found that copulation began in early July, with egg spawning occurring in early August. The sex ratio was female-biased until late August, then shifted to male-biased. After early August, the average number of sperm sacs in females exceeded that of males. As the season progresses, females began storing sperm sacs in a cryptic sperm pocket on the right lateral trunk within the mantle. This behavior is associated with male-biased sex ratio. These results suggest polyandry in <i>E. chunii.</i></p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome Sequence of Gordonia terrae Bacteriophage Wheezy.
microPublication biology Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001407
Rachel M Heyne, Catherine P Chia
{"title":"Genome Sequence of <i>Gordonia terrae</i> Bacteriophage Wheezy.","authors":"Rachel M Heyne, Catherine P Chia","doi":"10.17912/micropub.biology.001407","DOIUrl":"10.17912/micropub.biology.001407","url":null,"abstract":"<p><p>Bacteriophage Wheezy, a lytic phage with siphoviral morphology isolated using the host <i>Gordonia terrae</i> 3612, has a genome of 67,021 base pairs and is 65.9% GC. The genome sequence of Wheezy aligns most closely with subcluster CR2 phages Tracker and NatB6. Annotation of the full-length genome sequence of Phage Wheezy revealed 92 protein-coding genes and no tRNA genes.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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