microPublication biologyPub Date : 2025-03-18eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001496
Pamela R Hirschberg, Munir Rahbe, Kevin Knapp, Hamad Wajid, Vanessa H Routh, Pallabi Sarkar
{"title":"Bone morphogenetic protein 8B (BMP8B) increases the glucose sensitivity of ventromedial hypothalamus (VMH) glucose-inhibited (GI) neurons in female mice.","authors":"Pamela R Hirschberg, Munir Rahbe, Kevin Knapp, Hamad Wajid, Vanessa H Routh, Pallabi Sarkar","doi":"10.17912/micropub.biology.001496","DOIUrl":"10.17912/micropub.biology.001496","url":null,"abstract":"<p><p>In female, but not male, mice, bone morphogenetic protein (BMP) 8B affects energy homeostasis by inhibiting AMP activated protein kinase (AMPK) in the ventromedial hypothalamus (VMH). VMH glucose-inhibited (GI) neurons that express neuronal nitric oxide synthase (nNOS) increase blood glucose in an AMPK dependent fashion. We tested the hypothesis that BMP8B increases glucose inhibition on VMH nNOS-GI neurons. We found that more VMH nNOS neurons expressed the BMP8B receptor in females than males. Moreover, BMP8B blunted activation of VMH GI neurons in low glucose. Thus, VMH nNOS-GI neurons may mediate some of the metabolic effects of BMP8B in females.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11967928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-17eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001550
Victoria E Garrido, William G Outlaw, Amanda M Powell, Elizabeth T Ables
{"title":"SR Protein Kinase is expressed in <i>Drosophila</i> ovarian germline stem cells but is not essential for their self-renewal.","authors":"Victoria E Garrido, William G Outlaw, Amanda M Powell, Elizabeth T Ables","doi":"10.17912/micropub.biology.001550","DOIUrl":"10.17912/micropub.biology.001550","url":null,"abstract":"<p><p>Germline stem cells (GSCs) are necessary for oocyte production in <i>Drosophila</i> . GSC maintenance is regulated by intrinsic factors that promote their self-renewal. One such factor, the beta-importin, <i>Transportin-Serine/Arginine rich</i> , mediates nuclear import of serine/arginine-rich (SR) proteins, which are phosphorylated by SR protein kinases. Here, we investigate whether the kinase encoded by <i>SR protein kinase</i> ( <i>SRPK</i> ) is essential for GSC self-renewal. We find that SRPK is expressed in GSCs and their mitotically-dividing daughters, but is not necessary for GSC establishment or maintenance. We conclude that SRPK is dispensable for GSC self-renewal, and postulate that other protein kinases can compensate for its absence.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-16eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001491
Orin R Daspit, Andrew C Slagter, Drew VanWoerkom, Derby Effie Addison, Samantha M Arteaga, Rory E Barnard, Nick M Bultje, Junu Chung, Brenna M Coleman, Cameron D Figg, Audrey J Gonzales, Josiah M Hoekman, Kat H Irmen, Valerie G Krause, Natalie L Luginbill, Rebecca A Nulty, Alexander W Scofield, Marguerite C Sytsema, Charles E Tavera, Anna G Thomas, Simon P Troyer, Hannah Sheppard, Myles D Radersma, John T Wertz, Randall J DeJong
{"title":"Complete genome sequences of <i>Rhodococcus equi</i> phages CoffeeBean, Dorin, Francesca, Madraxi, and Tonitrus.","authors":"Orin R Daspit, Andrew C Slagter, Drew VanWoerkom, Derby Effie Addison, Samantha M Arteaga, Rory E Barnard, Nick M Bultje, Junu Chung, Brenna M Coleman, Cameron D Figg, Audrey J Gonzales, Josiah M Hoekman, Kat H Irmen, Valerie G Krause, Natalie L Luginbill, Rebecca A Nulty, Alexander W Scofield, Marguerite C Sytsema, Charles E Tavera, Anna G Thomas, Simon P Troyer, Hannah Sheppard, Myles D Radersma, John T Wertz, Randall J DeJong","doi":"10.17912/micropub.biology.001491","DOIUrl":"10.17912/micropub.biology.001491","url":null,"abstract":"<p><p>We report genomes of five phages isolated using the actinobacterium <i>Rhodococcus equi</i> NRRL B-16538 <i>.</i> Based on gene content similarity, one phage is assigned to actinobacteriphage cluster CF, one to cluster CR, two to cluster CG, and one that cannot be assigned to any existing cluster. The latter encodes a five-gene thymine hypermodification system.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-16eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001516
Emily Williams, Nico Pinzon, Grace Semrau, Vicky Pete, Mary Elizabeth Gabrielle, Rachid El Bejjani
{"title":"<i>rab-33</i> is not required for cuticle integrity in <i>Caenorhabditis elegans</i>.","authors":"Emily Williams, Nico Pinzon, Grace Semrau, Vicky Pete, Mary Elizabeth Gabrielle, Rachid El Bejjani","doi":"10.17912/micropub.biology.001516","DOIUrl":"10.17912/micropub.biology.001516","url":null,"abstract":"<p><p>Rab GTPases are master regulators of intracellular transport. We previously showed that a <i>rab-6.2</i> deletion leads to a compromised cuticle, glycosylation defects, and increased axon regeneration. Mammalian orthologs of <i>rab-6.2</i> and <i>rab-33</i> have been shown to mediate Golgi trafficking of cargo in mammalian cells, including that of glycosyltransferase enzymes. We engineered a novel STOP-IN putative null allele of <i>rab-33 ( axr2 )</i> to determine if loss of function of <i>rab-33</i> phenocopies the phenotypes seen in <i>rab-6.2 ( ok2254 ).</i> Our results suggest that <i>rab-33</i> is not required for cuticle integrity.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-15eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001471
Ibrahim Abdulrehman, Nathaly Angeles, Alexis Bostock, Ciara Burns, Srey Lim, Adrienne Nalley, Madison Olander, Preston Penny, Bailey Phipps, Jack Price, Jacob Rose, Gabrial Smith, Mackenzie Spalding, Sadie Weisman-Rosenberger, Pamela Connerly, Danielle Watt, Elizabeth Rueschhoff
{"title":"Genome Sequence of <i>Gordonia rubripertincta</i> Phage DoobyDoo of the DV Cluster.","authors":"Ibrahim Abdulrehman, Nathaly Angeles, Alexis Bostock, Ciara Burns, Srey Lim, Adrienne Nalley, Madison Olander, Preston Penny, Bailey Phipps, Jack Price, Jacob Rose, Gabrial Smith, Mackenzie Spalding, Sadie Weisman-Rosenberger, Pamela Connerly, Danielle Watt, Elizabeth Rueschhoff","doi":"10.17912/micropub.biology.001471","DOIUrl":"10.17912/micropub.biology.001471","url":null,"abstract":"<p><p>Novel bacteriophage DoobyDoo was isolated and characterized utilizing host <i>Gordonia rubripertincta</i> NRRL B-16540. DoobyDoo has a siphoviral morphology and a 66,343 bp genome with a GC content of 58.3%. The genome contains 97 protein coding genes, including an esterase gene that is distributed broadly across actinobacteriophages.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-15eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001307
Morghan McCool, Clarissa Molina, Lucas Oyarzún Contreras, Sebastian Zambrano Rojas, Carmen Burkett, Francesca Burkett, Ri Corwin, Issabella Serrani Gellego, Benton J Hendrickson, Desiree Jackson, Sara Joseph, Jonathan Lautenbach, Erin Todd, Felipe Morales Armijo, Michael Thompson, Laura Sanchez Jardon, Roy Mackenzie, Andrew Gregory
{"title":"Biocultural conservation trail system reduces bryophyte richness but not diversity in the southernmost miniature forest of the world.","authors":"Morghan McCool, Clarissa Molina, Lucas Oyarzún Contreras, Sebastian Zambrano Rojas, Carmen Burkett, Francesca Burkett, Ri Corwin, Issabella Serrani Gellego, Benton J Hendrickson, Desiree Jackson, Sara Joseph, Jonathan Lautenbach, Erin Todd, Felipe Morales Armijo, Michael Thompson, Laura Sanchez Jardon, Roy Mackenzie, Andrew Gregory","doi":"10.17912/micropub.biology.001307","DOIUrl":"10.17912/micropub.biology.001307","url":null,"abstract":"<p><p>The Cape Horn region of southern Chile is one of the remaining bryophyte (mosses, liverworts, and hornworts) hotspots in the world. The Omora Ethnobotanical Park on Navarino Island contains impressive examples of the region's bryophyte diversity. A new trail has been proposed and we aimed to predict how a trail expansion might impact bryophyte communities. We compared the current trail and the proposed trail site and found significant differences. Specifically, there was no significant difference in bryophyte cover and diversity, but richness was lower at the existing trail. These findings indicate that ecotourism trails may negatively impact bryophyte communities.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-14eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001462
Ian T Rife, Nana Sannomiya, Katie N Thai, Cori B Williams, Raelynn A Amasol-Tanoura, Jennifer H Arca, Gillian A Bradley, Avery C Catlin, Saige A Edwards, Sophia F Faria, Maximilliano Fuentes-Ayala, Lola M Gantenbein, Alexis M Haiges, Hailey E Hart, Ayden K Herrera, Paisley J Karlin, Landon H Schumaker, Emily J Velasquez, Hannah E Moon, Megan L Porter
{"title":"Genome Sequence of <i>Arthrobacter</i> Phage Sourignavong.","authors":"Ian T Rife, Nana Sannomiya, Katie N Thai, Cori B Williams, Raelynn A Amasol-Tanoura, Jennifer H Arca, Gillian A Bradley, Avery C Catlin, Saige A Edwards, Sophia F Faria, Maximilliano Fuentes-Ayala, Lola M Gantenbein, Alexis M Haiges, Hailey E Hart, Ayden K Herrera, Paisley J Karlin, Landon H Schumaker, Emily J Velasquez, Hannah E Moon, Megan L Porter","doi":"10.17912/micropub.biology.001462","DOIUrl":"10.17912/micropub.biology.001462","url":null,"abstract":"<p><p>We report the genome sequence of phage Sourignavong isolated from soil in Oklahoma City, Oklahoma using <i>Arthrobacter</i> sp. ATCC 21022. The 15,625 bp genome contains 27 predicted protein coding genes, including two predicted endolysin enzyme genes. Sourignavong is assigned to actinobacteriophage cluster AN.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-13eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001552
Joshua S Park, Lesley A Turner, Kathleen L Gould, Alaina H Willet
{"title":"Characterization of temperature-sensitive alleles of anillin-like Mid1 and polo kinase Plo1 in <i>Schizosaccharomyces pombe</i>.","authors":"Joshua S Park, Lesley A Turner, Kathleen L Gould, Alaina H Willet","doi":"10.17912/micropub.biology.001552","DOIUrl":"10.17912/micropub.biology.001552","url":null,"abstract":"<p><p>The <i>Schizosaccharomyces pombe</i> anillin-like Mid1 is important for the correct positioning of the cell division site. A key regulator of Mid1 is the polo kinase Plo1 which is important for several mitotic and cytokinetic events including spindle formation and division site placement. Here, we defined the mutations within a set of temperature-sensitive <i>mid1</i> and <i>plo1</i> alleles and compared the growth and morphological defects of the strains. This work expands the repertoire of <i>mid1</i> and <i>plo1</i> mutants for studying cytokinesis and highlights the requirement of the Mid1 C2 domain and the Plo1 kinase domain C-terminal lobe as particularly important for cytokinesis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-13eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001460
Ashley Gledhill, Gabriel S Gooden, Janelle Aguazul, Aanyaa Arora, Ava R Blackledge, Michael H Bucks, Tessa Carby, Andrew Cu, Jacob Fulkerson, Chealsea W Gachagua, Elly Grogan, Haley H Hanson, Gillian N Johnston, Lola S Norman, Lindsey M Oak, Grant J Oller, Het Parekh, Sakshi Patel, Sydney Putnam, Grant Spalding, Jacob Thomas, Patrick Wallace, Claire A Rinehart, Rodney A King
{"title":"Genome Sequences of Cluster E Mycobacteriophages Xandras and BigBubba.","authors":"Ashley Gledhill, Gabriel S Gooden, Janelle Aguazul, Aanyaa Arora, Ava R Blackledge, Michael H Bucks, Tessa Carby, Andrew Cu, Jacob Fulkerson, Chealsea W Gachagua, Elly Grogan, Haley H Hanson, Gillian N Johnston, Lola S Norman, Lindsey M Oak, Grant J Oller, Het Parekh, Sakshi Patel, Sydney Putnam, Grant Spalding, Jacob Thomas, Patrick Wallace, Claire A Rinehart, Rodney A King","doi":"10.17912/micropub.biology.001460","DOIUrl":"10.17912/micropub.biology.001460","url":null,"abstract":"<p><p>Bacteriophages Xandras and BigBubba were isolated on <i>Mycobacterium smegmatis</i> mc <sup>2</sup> 155 from enriched soil samples. Xandras' genome length is 75,179 bp with 144 predicted protein-coding genes and two tRNAs. BigBubba's genome length is 75,006 bp with 147 predicted protein-coding genes and two tRNAs. Each genome contains a cyclic oligonucleotide sequestering protein (CBASS antagonist).</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2025-03-12eCollection Date: 2025-01-01DOI: 10.17912/micropub.biology.001463
Youngwoo Kim, Jiwoo Kim, Victoria Spedding, Robin Cooper
{"title":"The potential impact of lipopolysaccharides (LPS) interactions on light-activated ion channels and pumps.","authors":"Youngwoo Kim, Jiwoo Kim, Victoria Spedding, Robin Cooper","doi":"10.17912/micropub.biology.001463","DOIUrl":"10.17912/micropub.biology.001463","url":null,"abstract":"<p><p>Optogenetics, the expression of light-activated ion channels and pumps, has the potential in therapeutic treatments for various pathologies. Thus, it is of interest to investigate pharmacological agents, immunological responses, and disease states like bacterial septicemia to learn if light-activated ion channels and pumps are compromised. Bacterial septicemia may impede the function of light-activated channels due to direct actions of lipopolysaccharides. This study explored the effects of activating light-sensitive channels (channelrhodopsin and halorhodopsin) before, during, and after exposure of lipopolysaccharides. Lipopolysaccharide itself did not directly impede the action of LPS but varied because of the effect on ion gradients by changes in membrane potential by lipopolysaccharides.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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