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Wild-Type Drosophila melanogaster Strains Respond Differentially to Rotenone Exposure.
microPublication biology Pub Date : 2025-01-27 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001380
Shiva Chaudhary, Shreyas Mohan Iyer, Meghana Tare
{"title":"Wild-Type <i>Drosophila melanogaster</i> Strains Respond Differentially to Rotenone Exposure.","authors":"Shiva Chaudhary, Shreyas Mohan Iyer, Meghana Tare","doi":"10.17912/micropub.biology.001380","DOIUrl":"10.17912/micropub.biology.001380","url":null,"abstract":"<p><p><i>Drosophila melanogaster</i> has been established as a reliable <i>in vivo</i> model for studying human diseases. However, the varied designs of such studies and the different origins of the strains have significantly contributed to metabolic and molecular differences between strains. Parkinson's disease (PD) is a neurodegenerative disorder involving the loss of dopaminergic neurons, leading to various motor and non-motor symptoms including but not limited to bradykinesia, postural instability, cognitive decline, and gut dysbiosis. Chronic exposure to toxins such as rotenone can induce neuronal cell death. We have developed a sporadic PD model by direct feeding of rotenone-supplemented food to <i>Drosophila melanogaster</i> wild-type strains, which has previously been shown to cause neuronal cell death and used to mimic PD in <i>Drosophila.</i> Upon exposure to rotenone in two wild-type strains ( <i>Oregon-R</i> and <i>Canton-S)</i> , differences in their climbing ability and lifespan were monitored. We found that the degree of motor defects upon rotenone exposure is higher in <i>Oregon-R</i> compared to age-matched <i>Canton-S</i> flies. We also observed that the <i>Canton-S</i> flies (rotenone-fed and non-rotenone-fed) exhibited a lower survival percentage than <i>Oregon-R</i> flies. However, the climbing defects in <i>Canton-S</i> flies are not as pronounced as in <i>Oregon-R</i> flies. The mechanism(s) involved in such differential effects in different wild-type <i>Drosophila</i> strains are yet to be explored and may provide a perspective on differential symptoms of PD patients belonging to different demographics.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular evidence supports the functionality of a protein-trapped endogenous allele of Dally-like protein.
microPublication biology Pub Date : 2025-01-25 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001283
Drew Delmore, Indrayani Waghmare
{"title":"Molecular evidence supports the functionality of a protein-trapped endogenous allele of Dally-like protein.","authors":"Drew Delmore, Indrayani Waghmare","doi":"10.17912/micropub.biology.001283","DOIUrl":"10.17912/micropub.biology.001283","url":null,"abstract":"<p><p>The <i>Drosophila</i> glypican Dally-like protein (Dlp) is an evolutionarily-conserved cell-surface protein that modulates extracellular distribution of several secreted ligands for cell signaling. Several fly lines expressing tagged <i>dlp</i> have been used to study the role of Dlp <i>in vivo</i> including the <i>PBac{602.P.SVS-1}dlp <sup>[CPTI000445]</sup></i> protein-trap line, which encodes StrepII-Venus-StrepII (SVS)-tagged Dlp from the endogenous locus. <i>dlp</i> is essential for embryonic development, and the <i>SVS-dlp</i> line is homozygous viable. Although this suggests that the SVS-tagged Dlp is functional, it is possible that that the <i>SVS-dlp</i> flies produce wild-type <i>dlp</i> isoform through alternative splicing, contributing to their survival. Here, we used a molecular analysis approach to show that the <i>SVS-dlp</i> flies do not produce wild-type isoform, confirming that the SVS-tagged Dlp is indeed functional.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heat Stress, Starvation, and Heat Stress Plus Starvation Cause Unique Transcriptomic Responses in the Economically Important Red Abalone Haliotis rufescens.
microPublication biology Pub Date : 2025-01-24 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001473
Hanna L Franklin, Lani U Gleason
{"title":"Heat Stress, Starvation, and Heat Stress Plus Starvation Cause Unique Transcriptomic Responses in the Economically Important Red Abalone <i>Haliotis rufescens</i>.","authors":"Hanna L Franklin, Lani U Gleason","doi":"10.17912/micropub.biology.001473","DOIUrl":"10.17912/micropub.biology.001473","url":null,"abstract":"<p><p>Although most marine invertebrates are experiencing multiple environmental stressors simultaneously, the transcriptome-wide gene expression responses to multiple stressors remain understudied. We used RNA-sequencing to assess the transcriptomic responses to heat stress, starvation, and heat stress plus starvation in the red abalone <i>Haliotis rufescens.</i> Results indicate that the response to each stressor is distinct and is characterized by unique gene functions. The heat stress plus starvation treatment produced the largest transcriptomic response, including a significant upregulation of genes involved in translation. Overall, this study highlights the importance of multi-stressor experiments that reflect the complex modalities of climate change.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143384343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dafadine Does Not Promote Dauer Development in Pristionchus pacificus.
microPublication biology Pub Date : 2025-01-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001470
Heather R Carstensen, Ray L Hong
{"title":"Dafadine Does Not Promote Dauer Development in <i>Pristionchus pacificus</i>.","authors":"Heather R Carstensen, Ray L Hong","doi":"10.17912/micropub.biology.001470","DOIUrl":"10.17912/micropub.biology.001470","url":null,"abstract":"<p><p>In response to unfavorable conditions, nematodes develop into the stress-resistant dauer larvae. Under favorable conditions, many nematodes are known to synthesize dafachronic acids (DAs) that bind to the conserved nuclear hormone receptor DAF-12 to suppress dauer development. However, the enzymes involved in the production of DAs have not been thoroughly investigated in <i>Pristionchus pacificus</i> . Here we show that the cytochrome P450 inhibitor Dafadine-A, which suppresses DAF-9 in DA biosynthesis in <i>C. elegans</i> and other nematode species, does not cause constitutive dauer formation or gonad migration defects in <i>P. pacificus</i> wild type. Instead, Dafadine-A may slightly reduce <i>P. pacificus</i> growth rate.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alpha-Synuclein Fails to Form Aggregates in Endocytosis-Defective Fission Yeast Strains, ∆ myo1 and ∆ end4.
microPublication biology Pub Date : 2025-01-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001479
Teruaki Takasaki, Ryuga Yamada, Yoshitaka Sugimoto, Reiko Sugiura
{"title":"Alpha-Synuclein Fails to Form Aggregates in Endocytosis-Defective Fission Yeast Strains, ∆ <i>myo1</i> and ∆ <i>end4</i>.","authors":"Teruaki Takasaki, Ryuga Yamada, Yoshitaka Sugimoto, Reiko Sugiura","doi":"10.17912/micropub.biology.001479","DOIUrl":"10.17912/micropub.biology.001479","url":null,"abstract":"<p><p>Alpha-Synuclein (α-Syn) is a soluble neuronal protein whose aggregation is one of the hallmarks of Parkinson's disease (PD). We previously developed a fission yeast model of PD that recapitulates α-Syn aggregation upon high-level expression of human α-Syn. Here, we show that α-Syn aggregate formation in yeast requires Myo1 and End4 , proteins essential for the early steps of endocytosis. α-Syn expression levels in Δ <i>myo1</i> and <i>∆end4</i> cells were comparable to wild-type cells, suggesting that defects in endocytosis disrupt α-Syn aggregation. These findings highlight the critical role of endocytosis in α-Syn aggregation and PD pathology.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11795301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low-fecundity dhc-1; mel-28 C. elegans mutants do not have gonad mitosis defects.
microPublication biology Pub Date : 2025-01-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001445
Julia Stobierska, Anita G Fernandez
{"title":"Low-fecundity <i>dhc-1; mel-28 C. elegans</i> mutants do not have gonad mitosis defects.","authors":"Julia Stobierska, Anita G Fernandez","doi":"10.17912/micropub.biology.001445","DOIUrl":"10.17912/micropub.biology.001445","url":null,"abstract":"<p><p>In <i>C. elegans , dhc-1 ( or283 ); mel-28 ( t1684 )</i> double mutants have a severely reduced brood size compared with each single mutant and compared to the wild type. To determine if this synthetic low-fecundity phenotype is due to reduced potential to produce gametes, we studied gonad length and distal gonad mitotic activity in <i>dhc-1 ( or283 )</i> mutants, <i>mel-28 ( t1684 )</i> mutants, wild-type animals, and <i>dhc-1 ( or283 ); mel-28 ( t1684 )</i> double mutants. Gonad length in <i>dhc-1 ; mel-28</i> double mutants was the same as the wild type. Using an antibody against phosphorylated histone H3 (PH3), we tracked mitotic activity in mutant and wild-type gonads. We found no significant difference in mitotic activity between the double mutant and the wild-type. These observations suggest that the reduced brood size in <i>dhc-1 ; mel-28</i> double mutants is not caused by a mitotically-inactive gonad and instead has a different and yet-to-be-determined basis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11795300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparing simulations of actin filament compression reveals tradeoff between computational cost and capturing supertwist.
microPublication biology Pub Date : 2025-01-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001347
Blair Lyons, Saurabh S Mogre, Karthik Vegesna, Jessica S Yu, Mark Hansen, Aadarsh Raghunathan, Graham T Johnson, Eran Agmon, Matthew Akamatsu
{"title":"Comparing simulations of actin filament compression reveals tradeoff between computational cost and capturing supertwist.","authors":"Blair Lyons, Saurabh S Mogre, Karthik Vegesna, Jessica S Yu, Mark Hansen, Aadarsh Raghunathan, Graham T Johnson, Eran Agmon, Matthew Akamatsu","doi":"10.17912/micropub.biology.001347","DOIUrl":"10.17912/micropub.biology.001347","url":null,"abstract":"<p><p>The dynamic bending and twisting of actin drives numerous cellular processes. To compare how different spatial scales in actin models capture these dynamics, we developed two models of actin filaments: one at monomer-scale using ReaDDy and one at fiber-scale using Cytosim. Simulating filament compression across a range of velocities, we found a divergence between the monomer- and fiber-scale simulations; notably, the monomer-scale simulations more effectively captured filament supertwist, characteristic of helical structure, but at a higher computational cost. Such comparisons can aid in designing more efficient and accurate multi-scale biological models. Interactive visualizations at https://simularium.github.io/subcell-website.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11795302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison among bright green fluorescent proteins in C. elegans.
microPublication biology Pub Date : 2025-01-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001447
Sydney Ko, Kota Mizumoto
{"title":"Comparison among bright green fluorescent proteins in <i>C. elegans</i>.","authors":"Sydney Ko, Kota Mizumoto","doi":"10.17912/micropub.biology.001447","DOIUrl":"10.17912/micropub.biology.001447","url":null,"abstract":"<p><p>Green fluorescent proteins (GFPs) are invaluable tools for visualizing cells and proteins across model systems. Efforts have been made to generate brighter fluorescent proteins such as eGFP, GFPnovo2, mNeonGreen, and mStayGold. Here, we generated single-copy knock-in <i>C. elegans</i> strains for these GFP variants and directly compared their brightness and photostability. We confirmed that mStayGold is brighter and more photostable than eGFP, GFPnovo2, and mNeonGreen, suggesting that it may hold advantages over other GFP variants in experiments where brightness and photostability are important factors.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel viruses in the families Iflaviridae and Partitiviridae associated with the common eastern firefly Photinus pyralis.
microPublication biology Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001385
Seth A Brewer, Meaghan J Adler, Mckayla M Martin, Paula Rozo-Lopez, Benjamin J Parker
{"title":"Novel viruses in the families <i>Iflaviridae</i> and <i>Partitiviridae</i> associated with the common eastern firefly <i>Photinus pyralis</i>.","authors":"Seth A Brewer, Meaghan J Adler, Mckayla M Martin, Paula Rozo-Lopez, Benjamin J Parker","doi":"10.17912/micropub.biology.001385","DOIUrl":"10.17912/micropub.biology.001385","url":null,"abstract":"<p><p>Fireflies are iconic insects that are under threat from environmental change. Knowledge of the viral diversity associated with natural firefly populations is important to our understanding of the basic biology of these insects and could be relevant to firefly conservation. We performed metatranscriptome sequencing of the Common Eastern Firefly ( <i>Photinus pyralis)</i> and assembled genomes for two new species of virus in the families Iflaviridae and Partitiviridae. We surveyed multiple individuals for these viruses using PCR, and we showed that both viruses are found at intermediate frequences in a natural population.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endogenous SH2B1 protein localizes to lamellipodia and filopodia: platinum replica electron-microscopy study.
microPublication biology Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001451
Maria Diakonova, Christin Carter-Su, Tatyana Svitkina
{"title":"Endogenous SH2B1 protein localizes to lamellipodia and filopodia: platinum replica electron-microscopy study.","authors":"Maria Diakonova, Christin Carter-Su, Tatyana Svitkina","doi":"10.17912/micropub.biology.001451","DOIUrl":"10.17912/micropub.biology.001451","url":null,"abstract":"<p><p>The widely expressed adapter protein SH2B1 was initially identified as a binding partner and substrate of tyrosine kinase JAK2. SH2B1β potentiates JAK2 activation in response to different ligands, including growth hormone, leptin and prolactin. SH2B1β has been implicated in cell motility and regulation of actin rearrangement in response to growth hormone, prolactin and platelet-derived growth factor. Here we use immunofluorescence and platinum replica electron-microscopy (PREM) technique to study localization of endogenous SH2B1. We show that endogenous SH2B localizes to two actin-rich protrusive organelles in cells: lamellipodia and filopodia. Based on this and previously published data, we suggest that at least some SH2B1 isoforms directly bind to actin filaments in both structures. Additionally, SH2B1 isoforms may work as a partner of filamin A in lamellipodia and VASP in filopodia participating in modulation of the actin cytoskeleton in response to extracellular signals.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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