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PITX2 is enriched in highly adipogenic brown preadipocytes. PITX2在高度成脂的棕色前脂肪细胞中富集。
microPublication biology Pub Date : 2025-07-24 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001570
Manal Al Dow, Charles Colas, Blandine Secco, Alona Kolnohuz, Nolwenn Samson, Audrey Poirier, Romain Villot, Laurence Belley, Bochra Chadel, Laura Tribouillard, Alyson Dufour, Mathilde Mouchiroud, Yves Gélinas, Mathieu C Morissette, Mathieu Laplante
{"title":"PITX2 is enriched in highly adipogenic brown preadipocytes.","authors":"Manal Al Dow, Charles Colas, Blandine Secco, Alona Kolnohuz, Nolwenn Samson, Audrey Poirier, Romain Villot, Laurence Belley, Bochra Chadel, Laura Tribouillard, Alyson Dufour, Mathilde Mouchiroud, Yves Gélinas, Mathieu C Morissette, Mathieu Laplante","doi":"10.17912/micropub.biology.001570","DOIUrl":"10.17912/micropub.biology.001570","url":null,"abstract":"<p><p>Brown adipose tissue (BAT) serves as a key heat-producing organ required to maintain body temperature and homeothermy in mammals. BAT recruitment and activation deeply impacts metabolic homeostasis in both mice and humans. Despite recent advances, the nature of the factors that functionally characterize brown adipose precursors are still incompletely characterized. Here, we provide a comprehensive transcriptomic analysis of brown preadipocyte cell lines with low or high adipogenic potential. Using this resource, we report the identification of paired-like homeodomain 2 (PITX2) as a transcription factor highly enriched in committed brown preadipocytes.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular docking of danuglipron uncovers potential crossovers between GLP-1R and the endocannabinoid system. 丹格列酮的分子对接揭示了GLP-1R和内源性大麻素系统之间的潜在交叉。
microPublication biology Pub Date : 2025-07-24 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001690
Kiersten A Dailey, Lillian N Schneider, Ruben C Petreaca, Ryan J Yoder
{"title":"Molecular docking of danuglipron uncovers potential crossovers between GLP-1R and the endocannabinoid system.","authors":"Kiersten A Dailey, Lillian N Schneider, Ruben C Petreaca, Ryan J Yoder","doi":"10.17912/micropub.biology.001690","DOIUrl":"10.17912/micropub.biology.001690","url":null,"abstract":"<p><p>The targeting of the G-protein coupled receptor (GPCRs) glucagon-like-peptide-1-receptor (GLP-1R) by weight loss medications has become extremely prevalent due to the effectiveness of a class of GLP-1R agonists. Also, interest in cannabinoids, such as delta-9-tetrahydrocannabinol (THC), has risen independently of GLP's newfound fame due to the relaxation of legal hurdles across the nation to recreational cannabis usage. THC interacts with receptors in the endocannabinoid system, with the major ones being cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2), both GPCRs. As these GPCR targets are becoming increasingly of interest due to these independent pathways, this study aimed to identify potential crossover between the endocannabinoid system and the GLP-1R system through molecular docking experiments. This was done using endogenous (2-AG and anandamide) and exogenous (THC) ligands of the endocannabinoid system , along with a proposed small oral agonist (danuglipron) of GLP-1R. Results indicated that danuglipron, a small GLP-1R agonist, had a higher binding affinity for CB1 and CB2 than any of the endogenous or exogenous ligands of the endocannabinoid system, suggesting the potential for cross-reactivity.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12364531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrafiltration as an alternative purification method for bovine serum albumin-fluorescein isothiocyanate bioconjugate. 超滤作为牛血清白蛋白-异硫氰酸荧光素生物偶联物的替代纯化方法。
microPublication biology Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001731
Catherine A Jackson, Elise L McKean, John M Hawdon
{"title":"Ultrafiltration as an alternative purification method for bovine serum albumin-fluorescein isothiocyanate bioconjugate.","authors":"Catherine A Jackson, Elise L McKean, John M Hawdon","doi":"10.17912/micropub.biology.001731","DOIUrl":"10.17912/micropub.biology.001731","url":null,"abstract":"<p><p>The bioconjugation of proteins with fluorescent probes is a popular method for protein visualization and has widespread applications across scientific fields. This study assessed ultrafiltration as an alternative purification method following the bioconjugation reaction that tags fluorescein isothiocyanate (FITC) to bovine serum albumin (BSA). Success of the purification was determined by (1) UV-Vis spectroscopy, comparing our sample to a commercially available product, and (2) use in an in vitro bioassay. Centrifugal ultrafiltration devices with a molecular weight cut off below the molecular weight of the conjugated protein were successful in retaining the purified product and clearing unreacted FITC from solution.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144801102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome sequence of Paracoccus sandarakinolimnes ME4, a pigmented, aerobic bacterium isolated from freshwater. 从淡水中分离出的一种色素好氧细菌——沙达拉金林副球菌ME4的基因组序列。
microPublication biology Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001660
Victoria Abramczuk, Virginia Saionz, Maria Estevez, Emily Stowe
{"title":"Genome sequence of <i>Paracoccus sandarakinolimnes</i> ME4, a pigmented, aerobic bacterium isolated from freshwater.","authors":"Victoria Abramczuk, Virginia Saionz, Maria Estevez, Emily Stowe","doi":"10.17912/micropub.biology.001660","DOIUrl":"10.17912/micropub.biology.001660","url":null,"abstract":"<p><p><i>Paracoccus</i> is a metabolically diverse genus found in a range of habitats. Here we describe <i>Paracoccus sandarakinolimnes</i> ME4, a carotenoid-producing, aerobic bacterium isolated from freshwater. The genome contains 4,061,481 bases in five contigs with 4,028 protein-coding genes, 106 tRNA and rRNA genes, and 35 pseudogenes.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence of brucellosis infection in the Milwaukee County Poor Farm Cemetery. 密尔沃基县贫困农场公墓发现布鲁氏菌感染的证据。
microPublication biology Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001601
Helen Werner
{"title":"Evidence of brucellosis infection in the Milwaukee County Poor Farm Cemetery.","authors":"Helen Werner","doi":"10.17912/micropub.biology.001601","DOIUrl":"10.17912/micropub.biology.001601","url":null,"abstract":"<p><p>Forty-seven skeletonized individuals from the Milwaukee County Poor Farm Cemetery (MCPFC) had DNA extracted from their dental calculus to look for the presence of respiratory pathogens. The MCPFC is curated by the Archaeology Laboratory at the University of Wisconsin-Milwaukee. According to the death records, respiratory infections and pneumonias were a leading cause of death. Polymerase Chain Reaction was run to examine the extracted DNA for evidence of <i>Mycobacterium tuberculosis</i> , <i>Brucella</i> species, and <i>Streptococcus pneumoniae</i> . Three individuals had IS711 present in their dental calculus, suggesting infection by <i>Brucella</i> species. This is the first documented brucellosis DNA successfully extracted from dental calculus.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UNC-52 localization with respect to longitudinal axon tracts in the ventral and dorsal nerve cords in C. elegans. 秀丽隐杆线虫腹背神经索纵向轴突束的UNC-52定位。
microPublication biology Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001703
Debapriya Roy, Harald Hutter
{"title":"UNC-52 localization with respect to longitudinal axon tracts in the ventral and dorsal nerve cords in <i>C. elegans</i>.","authors":"Debapriya Roy, Harald Hutter","doi":"10.17912/micropub.biology.001703","DOIUrl":"10.17912/micropub.biology.001703","url":null,"abstract":"<p><p>UNC-52 /Perlecan is a core basement membrane (BM) component essential for connecting muscle cells to overlying hypodermal cells. In the nervous system of <i>C. elegans</i> , <i>unc-52 ( ra515 )</i> causes dendritic branching defects in the PVD neuron independent of muscle attachment defects. We found that <i>unc-52 ( ra515 )</i> also causes axon navigation defects in the ventral nerve cord (VNC). We then examined the localization of UNC-52 with respect to ventral and dorsal nerve cord (DNC) axon tracts. We found that UNC-52 is localized underneath muscle cells as previously observed but is absent from BMs surrounding ventral and dorsal axon tracts at all stages of development.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314657/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple fixation for detection of endogenous fluorescent reporters in C. elegans that nearly eliminates intestinal autofluorescence. 一种用于检测秀丽隐杆线虫内源性荧光报告者的简单固定，几乎消除了肠道自身荧光。
microPublication biology Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001673
Ohm H Patel, Alexandra S Weisman, Craig P Hunter
{"title":"A simple fixation for detection of endogenous fluorescent reporters in <i>C. elegans</i> that nearly eliminates intestinal autofluorescence.","authors":"Ohm H Patel, Alexandra S Weisman, Craig P Hunter","doi":"10.17912/micropub.biology.001673","DOIUrl":"10.17912/micropub.biology.001673","url":null,"abstract":"<p><p>Gut granules are prominent cytoplasmic organelles that auto fluoresce when excited by much of the visible spectrum confounding fluorescence imaging of endogenous fluorescent reporters. We report a simple, chemical-free fixation method for <i>C. elegans</i> that preserves non-cytoplasmic GFP localization for months while nearly eliminating intestinal autofluorescence. To illustrate the utility and limitations of the method, we present representative images of live and heat-fixed worms expressing a variety of membrane and non-membrane localized GFP reporters expressed in diverse tissues, cells, and cellular organelles. We also describe our experience with various experimental parameters. The observed variability of non-membrane-anchored reporters suggests prudent adopters should empirically interrogate the signal fidelity of their specific reporters upon heat fixation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ferroptosis in the U87MG Human Glioblastoma Cell Line Induces Damage Associated Molecular Phenotypes. U87MG人胶质母细胞瘤细胞系铁下垂诱导损伤相关分子表型
microPublication biology Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001524
Leif Neitzel, Samantha Rea, Jessica Cornell, Charles Williams, Charles Hong
{"title":"Ferroptosis in the U87MG Human Glioblastoma Cell Line Induces Damage Associated Molecular Phenotypes.","authors":"Leif Neitzel, Samantha Rea, Jessica Cornell, Charles Williams, Charles Hong","doi":"10.17912/micropub.biology.001524","DOIUrl":"10.17912/micropub.biology.001524","url":null,"abstract":"<p><p>Glioblastomas are known as \"immune cold\" cancers with little induction of damage-associated molecular phenotypes (DAMPS). We previously described the induction of ferroptosis in glioblastoma cells using the small molecule, OGM, a specific inhibitor of GPR68. The ferroptotic cell death pathway has been reported to induce the release of DAMPS. Here, we show that induction of ferroptosis through both Erastin and OGM results in DAMPS in U87MG cells. This suggests that ferroptosis in human glioblastomas may be able to convert them to an \"immune hot\" cancer, increasing their susceptibility to immunotherapy. These findings highlight the immunogenic potential of causing ferroptosis in glioblastoma as a therapeutic mechanism of action.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Field Sampling of Zaprionus indianus in the Eastern United States in 2023-2024. 2023-2024年美国东部印度扎普利虫的野外抽样。
microPublication biology Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001707
Weston J Gray, Megan Stephenson, Alan O Bergland, Priscilla A Erickson
{"title":"Field Sampling of <i>Zaprionus indianus</i> in the Eastern United States in 2023-2024.","authors":"Weston J Gray, Megan Stephenson, Alan O Bergland, Priscilla A Erickson","doi":"10.17912/micropub.biology.001707","DOIUrl":"10.17912/micropub.biology.001707","url":null,"abstract":"<p><p>We conducted surveys for the invasive drosophilid <i>Zaprionus indianus</i> in the eastern United States in 2023 and 2024. We found no latitudinal trends in <i>Z. indianus</i> abundance, and the northern boundary for <i>Z. indianus</i> was variable between years. <i>Z. indianus</i> was rare in central and northern Florida in the spring, was not present on early season berry crops in Virginia, and had a restricted temperate growing season compared to other drosophilids, providing further support for the species' yearly recolonization of temperate areas. <i>Z. indianus</i> was also observed on native fruits in a natural area in Virginia.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In E. coli , the expression of mechanosensitive channels depends on environmental conditions. 在大肠杆菌中，机械敏感通道的表达取决于环境条件。
microPublication biology Pub Date : 2025-07-17 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001133
Sarah C Johnson, Sydney Hauth, Saumya Ramanathan, Hannah R Malcolm
{"title":"In <i>E. coli</i> , the expression of mechanosensitive channels depends on environmental conditions.","authors":"Sarah C Johnson, Sydney Hauth, Saumya Ramanathan, Hannah R Malcolm","doi":"10.17912/micropub.biology.001133","DOIUrl":"10.17912/micropub.biology.001133","url":null,"abstract":"<p><p>Bacterial mechanosensitive ion channels are essential for cell survival in response to osmotic downshock, these channels rescue bacteria from lethal pressure changes. In <i>Escherichia coli</i> ( <i>E. coli</i> ), the genome encodes for a single copy of MscL and six MscS superfamily members (MscS, MscK, MscM, YnaI, YbdG, and YbiO). The majority of these have been shown to gate in response to tension. Here, we determined the relative expression using qPCR of all seven genes in non-modified <i>E. coli</i> cultured in a variety of different conditions. In all conditions, the highest expressed genes directly correlate with the most frequently observed channels in patch clamp electrophysiology: MscS, MscM, and MscL.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12314656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144777091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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