裂糖酵母抗青霉素样Mid1和polo激酶Plo1温度敏感等位基因的鉴定。

microPublication biology Pub Date : 2025-03-13 eCollection Date: 2025-01-01 DOI:10.17912/micropub.biology.001552
Joshua S Park, Lesley A Turner, Kathleen L Gould, Alaina H Willet
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引用次数: 0

摘要

Schizosaccharomyces pombe anillin-like Mid1对于细胞分裂位点的正确定位很重要。Mid1的一个关键调控因子是polo激酶Plo1,它对包括纺锤体形成和分裂位点定位在内的几个有丝分裂和细胞动力学事件很重要。在这里,我们定义了一组温度敏感的mid1和plo1等位基因内的突变,并比较了菌株的生长和形态缺陷。这项工作扩大了mid1和plo1突变体的库,用于研究细胞质分裂,并强调了mid1 C2结构域和plo1激酶结构域c端叶对细胞质分裂的特别重要的要求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of temperature-sensitive alleles of anillin-like Mid1 and polo kinase Plo1 in Schizosaccharomyces pombe.

The Schizosaccharomyces pombe anillin-like Mid1 is important for the correct positioning of the cell division site. A key regulator of Mid1 is the polo kinase Plo1 which is important for several mitotic and cytokinetic events including spindle formation and division site placement. Here, we defined the mutations within a set of temperature-sensitive mid1 and plo1 alleles and compared the growth and morphological defects of the strains. This work expands the repertoire of mid1 and plo1 mutants for studying cytokinesis and highlights the requirement of the Mid1 C2 domain and the Plo1 kinase domain C-terminal lobe as particularly important for cytokinesis.

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