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A Comparative Study of Life History Traits in C. briggsae and C. elegans. C.briggsae和C.elegans生活史特征的比较研究
microPublication biology Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001299
Nikita Jhaveri, Harvir Bhullar, Bhagwati Gupta
{"title":"A Comparative Study of Life History Traits in <i>C. briggsae</i> and <i>C. elegans</i>.","authors":"Nikita Jhaveri, Harvir Bhullar, Bhagwati Gupta","doi":"10.17912/micropub.biology.001299","DOIUrl":"10.17912/micropub.biology.001299","url":null,"abstract":"<p><p>The nematodes <i>C. elegans</i> and <i>C. briggsae</i> are key models for genetic studies. Despite their overall similar morphology, these two species exhibit notable differences. We used the isolates from tropical ( AF16 and QX1410 ) and temperate ( HK104 and VX34 ) regions to characterize the life history traits of <i>C. briggsae</i> . Our findings reveal significant variations in body dimensions, movement patterns, utse morphology, and lipid contents across isolates, highlighting species-level distinctions that further establish <i>C. briggsae</i> as a valuable comparative model for genetic research.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The atypical soluble guanylyl cyclase subunit Gyc89Db does not control neuroepithelial proliferation in Drosophila larval brain. 非典型可溶性鸟苷酸环化酶亚基Gyc89Db不能控制果蝇幼虫大脑神经上皮细胞的增殖
microPublication biology Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001336
Mariel Rosas, Rafael Cantera, Daniel Prieto
{"title":"The atypical soluble guanylyl cyclase subunit Gyc89Db does not control neuroepithelial proliferation in <i>Drosophila</i> larval brain.","authors":"Mariel Rosas, Rafael Cantera, Daniel Prieto","doi":"10.17912/micropub.biology.001336","DOIUrl":"10.17912/micropub.biology.001336","url":null,"abstract":"<p><p>We investigated the role of oxygen-sensing atypical guanylyl cyclase subunit Gyc89Db in the developing brain. Despite its expression in the hypoxic neuroepithelium of the larval optic lobe of <i>Drosophila</i> , loss-of-function mutants and ectopic expression did not alter neuroepithelial cell number or proliferation. Notably, while ectopic expression of <i>Gyc89Db</i> increases optic lobe volume and neuroblast numbers, our negative results suggest that these effects manifest earlier in development without persistent alteration of the neuroepithelium, through mechanisms that might be independent of neuroepithelial proliferation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human SVIP is sufficient to stimulate tubular lysosomes and extend healthspan in well-fed Caenorhabditis elegans. 人类 SVIP 足以刺激管状溶酶体，并延长喂养良好的秀丽隐杆线虫的健康寿命。
microPublication biology Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001379
Joshua P Gill, Kathryn R DeLeo, K Adam Bohnert, Alyssa E Johnson
{"title":"Human <i>SVIP</i> is sufficient to stimulate tubular lysosomes and extend healthspan in well-fed <i>Caenorhabditis elegans</i>.","authors":"Joshua P Gill, Kathryn R DeLeo, K Adam Bohnert, Alyssa E Johnson","doi":"10.17912/micropub.biology.001379","DOIUrl":"10.17912/micropub.biology.001379","url":null,"abstract":"<p><p>Small VCP Interacting Protein (SVIP) is essential for maintaining a unique form of tubular lysosomes (TLs) in <i>Drosophila</i> . Although <i>Caenorhabditis elegans</i> do not have an annotated <i>SVIP</i> ortholog, expression of <i>Drosophila SVIP</i> in the <i>C. elegans</i> intestine induces TLs constitutively, increases autophagic activity, and extends healthspan. Here, we find that expression of the human ortholog of <i>SVIP</i> in the <i>C. elegans</i> gut causes similar physiological and phenotypic effects as <i>Drosophila SVIP</i> , albeit some effects were less pronounced. These results demonstrate that human <i>SVIP</i> can induce functional TLs in <i>C. elegans</i> but may be a weaker allele compared to <i>Drosophila SVIP</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human plasma inositol hexakisphosphate (InsP 6 ) phosphatase identified as the Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1). 人血浆肌醇六磷酸（inp6）磷酸酶鉴定为多肌醇多磷酸磷酸酶1 （MINPP1）。
microPublication biology Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001390
Valeria Fedeli, Jingyi Wang, Vincent Cantagrel, Adolfo Saiardi
{"title":"Human plasma inositol hexakisphosphate (InsP <sub>6</sub> ) phosphatase identified as the Multiple Inositol Polyphosphate Phosphatase 1 (MINPP1).","authors":"Valeria Fedeli, Jingyi Wang, Vincent Cantagrel, Adolfo Saiardi","doi":"10.17912/micropub.biology.001390","DOIUrl":"10.17912/micropub.biology.001390","url":null,"abstract":"<p><p>Inositol hexakisphosphate (InsP <sub>6</sub> ), also known as phytic acid, is a potent chelator of bivalent cations. Intracellular InsP <sub>6</sub> molecules are associated with magnesium. Calcium is the prevalent bivalent cation outside the cell and its association with InsP <sub>6</sub> could lead to the formation of insoluble complexes. To avoid the formation of dangerous InsP <sub>6</sub> /Calcium precipitates in the bloodstream, mammals must possess a robust InsP <sub>6</sub> phosphatase in their plasma. Here we identify the Multiple Inositol Polyphosphate Phosphatase 1 ( MINPP1 ) as the InsP <sub>6</sub> phosphatase present in human plasma.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of the genome-editing activity of microinjected CRISPR/Cas9 ribonucleoprotein complexes in Daphnia pulex. 分析微注射 CRISPR/Cas9 核糖核蛋白复合物在水蚤中的基因组编辑活性。
microPublication biology Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001310
Megan E Maar, Jay C Miller, Michael Lynch, Andrew C Zelhof
{"title":"Analysis of the genome-editing activity of microinjected CRISPR/Cas9 ribonucleoprotein complexes in <i>Daphnia pulex</i>.","authors":"Megan E Maar, Jay C Miller, Michael Lynch, Andrew C Zelhof","doi":"10.17912/micropub.biology.001310","DOIUrl":"10.17912/micropub.biology.001310","url":null,"abstract":"<p><p>Although <i>Daphnia</i> is a widely used model organism with a completely sequenced genome, molecular tools for analyzing specific gene functions are still being developed. Progress has been made in developing CRISPR/Cas9 gene editing in <i>Daphnia</i> . However, the gene-editing activity of injected ribonucleoprotein complexes (RNPs), the success of co-injected RNPs with different gRNAs, and the heritability of mutations in asexual progeny need further investigation. Here, we show prolonged Cas9 RNP activity past the one-cell stage injected individuals, leading to a wide range of somatic mutations, and germline mosaicism of heritable biallelic mutations.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Grafting glycoprotein-derived oligosaccharides structures onto non-glycosylated polypeptides. 将糖蛋白衍生的寡糖结构嫁接到非糖基化多肽上。
microPublication biology Pub Date : 2024-10-30 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001314
Danya Medina-Carrasco, Lisandra García de Castro Cuspineda, Luis Gabriel González-Lodeiro, Satomy Pousa, Miladys Limonta, Vivian Huerta Galindo
{"title":"Grafting glycoprotein-derived oligosaccharides structures onto non-glycosylated polypeptides.","authors":"Danya Medina-Carrasco, Lisandra García de Castro Cuspineda, Luis Gabriel González-Lodeiro, Satomy Pousa, Miladys Limonta, Vivian Huerta Galindo","doi":"10.17912/micropub.biology.001314","DOIUrl":"10.17912/micropub.biology.001314","url":null,"abstract":"<p><p>Properties of recombinant glycoproteins can be altered by the addition of oligosaccharide structures specific to the cells used for its heterologous expression. A methodology was implemented to obtain a glycopeptide preparation useful to elucidate the role of carbohydrates in the immunogenicity and antigenicity of glycoproteins. It consists on the digestion of the protein, followed by selective capture of the oligosaccharides bound to di-/tripeptides, and their grafting onto a non-glycosylated receptor protein by chemical crosslinking. Glycopeptides derived from C-RBD-H6 PP protein, the active ingredient of the Abdala vaccine were efficiently grafted onto a non-glycosylated protein as evidenced by western blotting.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11561553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trapping of yFACT at 3' ends of genes is not a universal characteristic of yeast versions of Bryant-Li-Bhoj syndrome histone H3 mutants. 在基因 3' 端捕获 yFACT 并不是布赖恩特-李-波杰综合征组蛋白 H3 突变体酵母版本的普遍特征。
microPublication biology Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001384
Joseph S Beard, Lillian K Francis, Reece C Forrest, Agustin Kalinowski, Jackson C Parks, William H Griffin, Caroline L Tackett, Andrea A Duina
{"title":"Trapping of yFACT at 3' ends of genes is not a universal characteristic of yeast versions of Bryant-Li-Bhoj syndrome histone H3 mutants.","authors":"Joseph S Beard, Lillian K Francis, Reece C Forrest, Agustin Kalinowski, Jackson C Parks, William H Griffin, Caroline L Tackett, Andrea A Duina","doi":"10.17912/micropub.biology.001384","DOIUrl":"https://doi.org/10.17912/micropub.biology.001384","url":null,"abstract":"<p><p>Bryant-Li-Bhoj syndrome (BLBS) is associated with germline mutations in the genes encoding human histone H3.3. While to date 70 H3.3 mutants have been associated with BLBS, the molecular mechanisms underpinning this condition remain undefined. We recently showed that in yeast the H3-L61R BLBS mutant causes trapping of yFACT at 3' ends of genes, raising the possibility that this defect could be a contributing factor to disease across all H3-BLBS mutants. Here, we show that of nine additional yeast H3-BLBS mutants analyzed, only one causes yFACT 3' end-trapping, thus indicating that this defect is not a universal feature of H3-BLBS mutants. We also present additional phenotypic data that could provide insights into the molecular mechanisms contributing to BLBS in human patients.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LPIN2 is the phosphatase dominating the penultimate step of neutral lipid biosynthesis in Dictyostelium. LPIN2 是一种磷酸酶，主导着竹荪中性脂质生物合成的倒数第二步。
microPublication biology Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001296
Frederik Kappelt, Markus Maniak
{"title":"LPIN2 is the phosphatase dominating the penultimate step of neutral lipid biosynthesis in <i>Dictyostelium</i>.","authors":"Frederik Kappelt, Markus Maniak","doi":"10.17912/micropub.biology.001296","DOIUrl":"https://doi.org/10.17912/micropub.biology.001296","url":null,"abstract":"<p><p><i>Dictyostelium</i> amoebae store surplus fatty acids from the diet in form of lipid droplets. Some of the enzymes governing neutral lipid synthesis are already known. For the phosphatidic acid-specific phosphatases, six genes were found, one of which was automatically annotated as LPIN2. Two GFP-tagged variants of LPIN2 homogeneously distribute in the cytoplasm and no organelle association was observed. <i>LPIN2 <sup>-</sup></i> mutants contain less than 17% residual amount of the major neutral lipid species, but phospholipid amounts are not obviously affected. A growth retardation on bacteria as food source may suggest that lipid droplets serve to detoxify excess free fatty acids.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ets-10p::gfp expression is predictive of dauer formation in daf-16; daf-7 larvae. ets-10p::gfp 的表达可预测 daf-16; daf-7 幼虫的矮小症形成。
microPublication biology Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001358
Juanez T Lindsay, Matthew J Wirick, Xantha Karp
{"title":"<i>ets-10p::gfp</i> expression is predictive of dauer formation in <i>daf-16; daf-7</i> larvae.","authors":"Juanez T Lindsay, Matthew J Wirick, Xantha Karp","doi":"10.17912/micropub.biology.001358","DOIUrl":"10.17912/micropub.biology.001358","url":null,"abstract":"<p><p>In adverse conditions, <i>Caenorhabditis elegans</i> larvae can enter the alternative L2d stage. If conditions remain poor, L2d larvae can molt into stress-resistant dauer larvae. The FOXO ortholog <i>daf-16</i> promotes dauer formation, but <i>daf-16</i> mutants can enter dauer with incomplete penetrance in combination with a mutation in <i>daf-7</i> /TGFβ. The degree to which <i>daf-16 ; daf-7</i> larvae enter L2d is unknown. Here we show that many <i>daf-16 ; daf-7</i> mutants express intermediate levels of the <i>ets-10p::gfp</i> L2d marker, suggesting incomplete entry into L2d. Furthermore, lack of <i>ets-10p::gfp</i> expression early in the second larval stage partially predicts which <i>daf-16 ; daf-7</i> larvae will bypass dauer formation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell type-specific regulation by different cytokinetic pathways in the early embryo. 早期胚胎中不同细胞运动途径对细胞类型的特异性调控
microPublication biology Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001316
Caroline Q Connors, Sophia L Martin, Julien Dumont, Mimi Shirasu-Hiza, Julie C Canman
{"title":"Cell type-specific regulation by different cytokinetic pathways in the early embryo.","authors":"Caroline Q Connors, Sophia L Martin, Julien Dumont, Mimi Shirasu-Hiza, Julie C Canman","doi":"10.17912/micropub.biology.001316","DOIUrl":"10.17912/micropub.biology.001316","url":null,"abstract":"<p><p>Cytokinesis, the physical division of one cell into two, is typically assumed to use the same molecular process across animal cells. However, regulation of cell division can vary significantly among different cell types, even within the same multicellular organism. Using six fast-acting temperature-sensitive (ts) cytokinesis-defective mutants, we found that each had unique cell type-specific profiles in the early 2-cell through 8-cell <i>C. elegans</i> embryo. Certain cell types were more sensitive than others to actomyosin and spindle signaling disruptions, disrupting two members of the same complex could result in different phenotypes, and protection against actomyosin inhibition did not always protect against spindle signaling inhibition.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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