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Trapping of yFACT at 3' ends of genes is not a universal characteristic of yeast versions of Bryant-Li-Bhoj syndrome histone H3 mutants. 在基因 3' 端捕获 yFACT 并不是布赖恩特-李-波杰综合征组蛋白 H3 突变体酵母版本的普遍特征。
microPublication biology Pub Date : 2024-10-25 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001384
Joseph S Beard, Lillian K Francis, Reece C Forrest, Agustin Kalinowski, Jackson C Parks, William H Griffin, Caroline L Tackett, Andrea A Duina
{"title":"Trapping of yFACT at 3' ends of genes is not a universal characteristic of yeast versions of Bryant-Li-Bhoj syndrome histone H3 mutants.","authors":"Joseph S Beard, Lillian K Francis, Reece C Forrest, Agustin Kalinowski, Jackson C Parks, William H Griffin, Caroline L Tackett, Andrea A Duina","doi":"10.17912/micropub.biology.001384","DOIUrl":"https://doi.org/10.17912/micropub.biology.001384","url":null,"abstract":"<p><p>Bryant-Li-Bhoj syndrome (BLBS) is associated with germline mutations in the genes encoding human histone H3.3. While to date 70 H3.3 mutants have been associated with BLBS, the molecular mechanisms underpinning this condition remain undefined. We recently showed that in yeast the H3-L61R BLBS mutant causes trapping of yFACT at 3' ends of genes, raising the possibility that this defect could be a contributing factor to disease across all H3-BLBS mutants. Here, we show that of nine additional yeast H3-BLBS mutants analyzed, only one causes yFACT 3' end-trapping, thus indicating that this defect is not a universal feature of H3-BLBS mutants. We also present additional phenotypic data that could provide insights into the molecular mechanisms contributing to BLBS in human patients.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LPIN2 is the phosphatase dominating the penultimate step of neutral lipid biosynthesis in Dictyostelium. LPIN2 是一种磷酸酶，主导着竹荪中性脂质生物合成的倒数第二步。
microPublication biology Pub Date : 2024-10-23 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001296
Frederik Kappelt, Markus Maniak
{"title":"LPIN2 is the phosphatase dominating the penultimate step of neutral lipid biosynthesis in <i>Dictyostelium</i>.","authors":"Frederik Kappelt, Markus Maniak","doi":"10.17912/micropub.biology.001296","DOIUrl":"https://doi.org/10.17912/micropub.biology.001296","url":null,"abstract":"<p><p><i>Dictyostelium</i> amoebae store surplus fatty acids from the diet in form of lipid droplets. Some of the enzymes governing neutral lipid synthesis are already known. For the phosphatidic acid-specific phosphatases, six genes were found, one of which was automatically annotated as LPIN2. Two GFP-tagged variants of LPIN2 homogeneously distribute in the cytoplasm and no organelle association was observed. <i>LPIN2 <sup>-</sup></i> mutants contain less than 17% residual amount of the major neutral lipid species, but phospholipid amounts are not obviously affected. A growth retardation on bacteria as food source may suggest that lipid droplets serve to detoxify excess free fatty acids.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ets-10p::gfp expression is predictive of dauer formation in daf-16; daf-7 larvae. ets-10p::gfp 的表达可预测 daf-16; daf-7 幼虫的矮小症形成。
microPublication biology Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001358
Juanez T Lindsay, Matthew J Wirick, Xantha Karp
{"title":"<i>ets-10p::gfp</i> expression is predictive of dauer formation in <i>daf-16; daf-7</i> larvae.","authors":"Juanez T Lindsay, Matthew J Wirick, Xantha Karp","doi":"10.17912/micropub.biology.001358","DOIUrl":"10.17912/micropub.biology.001358","url":null,"abstract":"<p><p>In adverse conditions, <i>Caenorhabditis elegans</i> larvae can enter the alternative L2d stage. If conditions remain poor, L2d larvae can molt into stress-resistant dauer larvae. The FOXO ortholog <i>daf-16</i> promotes dauer formation, but <i>daf-16</i> mutants can enter dauer with incomplete penetrance in combination with a mutation in <i>daf-7</i> /TGFβ. The degree to which <i>daf-16 ; daf-7</i> larvae enter L2d is unknown. Here we show that many <i>daf-16 ; daf-7</i> mutants express intermediate levels of the <i>ets-10p::gfp</i> L2d marker, suggesting incomplete entry into L2d. Furthermore, lack of <i>ets-10p::gfp</i> expression early in the second larval stage partially predicts which <i>daf-16 ; daf-7</i> larvae will bypass dauer formation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell type-specific regulation by different cytokinetic pathways in the early embryo. 早期胚胎中不同细胞运动途径对细胞类型的特异性调控
microPublication biology Pub Date : 2024-10-22 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001316
Caroline Q Connors, Sophia L Martin, Julien Dumont, Mimi Shirasu-Hiza, Julie C Canman
{"title":"Cell type-specific regulation by different cytokinetic pathways in the early embryo.","authors":"Caroline Q Connors, Sophia L Martin, Julien Dumont, Mimi Shirasu-Hiza, Julie C Canman","doi":"10.17912/micropub.biology.001316","DOIUrl":"10.17912/micropub.biology.001316","url":null,"abstract":"<p><p>Cytokinesis, the physical division of one cell into two, is typically assumed to use the same molecular process across animal cells. However, regulation of cell division can vary significantly among different cell types, even within the same multicellular organism. Using six fast-acting temperature-sensitive (ts) cytokinesis-defective mutants, we found that each had unique cell type-specific profiles in the early 2-cell through 8-cell <i>C. elegans</i> embryo. Certain cell types were more sensitive than others to actomyosin and spindle signaling disruptions, disrupting two members of the same complex could result in different phenotypes, and protection against actomyosin inhibition did not always protect against spindle signaling inhibition.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Spatial Differences in Two Misfolded Proteins During Aggresome Formation. 两种错误折叠蛋白质在聚集体形成过程中的空间差异特征。
microPublication biology Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001312
Jordan N Goldy, Robert T Youker
{"title":"Characterization of Spatial Differences in Two Misfolded Proteins During Aggresome Formation.","authors":"Jordan N Goldy, Robert T Youker","doi":"10.17912/micropub.biology.001312","DOIUrl":"10.17912/micropub.biology.001312","url":null,"abstract":"<p><p>Cells have evolved an elaborate network of folding and degradation pathways to maintain the native state of proteins. If these pathways are disrupted (e.g., mutation) or their capacity is exceeded then protein aggregates form in cells. Cells sequester these aggregated proteins into aggresome or aggresome-like bodies as a protective mechanism. In this study, we co-expressed two model misfolded proteins in HEK293 cells and measured aggresome formation upon proteasomal inhibition. We observed spatial differences in early time points of aggresome formation upon co-expression of the misfolded proteins compared to individual expression in cells.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial aspartate aminotransferase ( maa1 ) inactivation causes glutamate-requiring glu1 mutation in Schizosaccharomyces pombe. 线粒体天门冬氨酸氨基转移酶（maa1）失活导致球囊酵母发生谷氨酸需要的 glu1 突变。
microPublication biology Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001338
Kenji Kitamura
{"title":"Mitochondrial aspartate aminotransferase ( <i>maa1</i> ) inactivation causes glutamate-requiring <i>glu1</i> mutation in <i>Schizosaccharomyces</i> <i>pombe</i>.","authors":"Kenji Kitamura","doi":"10.17912/micropub.biology.001338","DOIUrl":"10.17912/micropub.biology.001338","url":null,"abstract":"<p><p>Two genomic genes, which rescue ammonium assimilation defect in the glutamate-requiring <i>Schizosaccharomyces</i> <i>pombe glu1</i> mutant, were identified. The <i>maa1</i> , encoding a mitochondrial aspartate aminotransferase, is the causative gene of <i>glu1</i> mutation because an inseparable linkage between <i>maa1</i> and <i>glu1</i> on the chromosome, and also the <i>glu1</i> mutant strain has a nonsense mutation within the <i>maa1</i> coding region, which is responsible for its defective phenotype. The <i>yhm2</i> , a mitochondrial 2-oxoglutarate carrier, was also isolated as a weak multicopy suppressor gene. These findings reiterate the importance of the mitochondria in utilizing the amino acids for cellular nitrogen metabolism.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial respiratory capacity is not altered in aging rat brains with or without memory impairment. 无论是否存在记忆损伤，衰老大鼠大脑的线粒体呼吸能力都不会发生改变。
microPublication biology Pub Date : 2024-10-21 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001359
Pamela J Yao, Jeffrey M Long, Peter R Rapp, Dimitrios Kapogiannis
{"title":"Mitochondrial respiratory capacity is not altered in aging rat brains with or without memory impairment.","authors":"Pamela J Yao, Jeffrey M Long, Peter R Rapp, Dimitrios Kapogiannis","doi":"10.17912/micropub.biology.001359","DOIUrl":"10.17912/micropub.biology.001359","url":null,"abstract":"<p><p>Mitochondria are essential for supporting the high metabolic demands that are required for brain function. Impairments in mitochondria have been linked to age-related decline in brain functions. Here, we investigate whether the mitochondrial respiratory capacity of brain cells is changed in cognitive aging. We used a rat model of normal cognitive aging and analyzed mitochondrial oxidative phosphorylation in frozen brain samples. Mitochondrial oxygen consumption rate analysis of the frontal cortex did not show any differences between young rats and aged rats with either intact memory or impaired spatial memory. Mitochondrial ATP synthase activity and quantity also did not differ between young and aged rats. These results suggest that the total level of mitochondrial respiratory capacity is preserved in the frontal cortex of aged rats and may not explain aging-associated cognitive impairment.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Worms love Coffee too! Characterizing the neural substrates that regulate odor-guided responses to coffee. 蠕虫也爱咖啡调节对咖啡的气味引导反应的神经基质的特征。
microPublication biology Pub Date : 2024-10-18 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001242
Ashley Vega, Alexis Chua, Annabelle Tran, Amber Seader, Emily Chang, Liz Ayala, Adriana Torres, Gianina Pontrelli, Gareth Harris
{"title":"Worms love Coffee too! Characterizing the neural substrates that regulate odor-guided responses to coffee.","authors":"Ashley Vega, Alexis Chua, Annabelle Tran, Amber Seader, Emily Chang, Liz Ayala, Adriana Torres, Gianina Pontrelli, Gareth Harris","doi":"10.17912/micropub.biology.001242","DOIUrl":"10.17912/micropub.biology.001242","url":null,"abstract":"<p><p>The coffee industry reaches over 80 billion US dollars in revenue partially due to the numerous chemicals that allow for coffee's highly attractive aroma and overall flavor. Many people integrate coffee into their everyday routine; therefore, understanding the attraction to coffee can facilitate, 1) the characterization of its attractive nature, and, 2) allow further understanding of how humans interpret taste and smell on a molecular and cellular level, from initial sensation to higher processing of these complex neural signals. We report that the model worm, <i>C. elegans</i> , can smell and perform strong attraction behavior using chemotaxis towards various types of coffee odors. In this study, we show that the nematode <i>C. elegans</i> is strongly attracted to various forms of coffee. We have also identified neuronal molecules that mediate this sensory-dependent behavior. Overall, we provide a platform to more thoroughly dissect the mechanisms and neuronal circuits that mediate odor-guided behavior to a complex human-sensed stimulus.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of a novel downstream single-minded midline regulatory element in Drosophila melanogaster. 鉴定黑腹果蝇中一个新的下游单一中线调控元件
microPublication biology Pub Date : 2024-10-17 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001317
Geyenna Sterling-Lentsch, Marc S Halfon
{"title":"Identification of a novel downstream <i>single-minded</i> midline regulatory element in <i>Drosophila melanogaster</i>.","authors":"Geyenna Sterling-Lentsch, Marc S Halfon","doi":"10.17912/micropub.biology.001317","DOIUrl":"10.17912/micropub.biology.001317","url":null,"abstract":"<p><p>Development of the <i>Drosophila melanogaster</i> central nervous system midline depends on the gene <i>single-minded</i> ( <i>sim</i> ). Although <i>sim</i> regulation has been studied extensively, the fact that an enhancer mediating late embryonic <i>sim</i> transcription has not been identified suggests that additional regulatory sequences remain unknown. We tested several evolutionarily conserved sequences in the <i>sim</i> downstream region and isolated <i>sim_3pB</i> , whose midline activity in a reporter gene assay begins later than previously characterized <i>sim</i> enhancers. Its activity shares several key similarities with the <i>Aedes aegypti sim</i> _ <i>5P3</i> enhancer, though is sufficiently different to warrant further investigation into how <i>sim_3pB</i> functions in its native context.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
miR-127/3p Inhibits Cell Migration in Lung Adenocarcinoma Under Hypoxic and Normal Oxygen Conditions. miR-127/3p 在缺氧和正常氧条件下抑制肺腺癌的细胞迁移
microPublication biology Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001355
Jackson Lipscomb, Kassidy Gray, Tuesday Melton, Parker Nelson, Alyssa Rye, Christin L Pruett, Nathan S Reyna
{"title":"miR-127/3p Inhibits Cell Migration in Lung Adenocarcinoma Under Hypoxic and Normal Oxygen Conditions.","authors":"Jackson Lipscomb, Kassidy Gray, Tuesday Melton, Parker Nelson, Alyssa Rye, Christin L Pruett, Nathan S Reyna","doi":"10.17912/micropub.biology.001355","DOIUrl":"10.17912/micropub.biology.001355","url":null,"abstract":"<p><p>MicroRNAs are small noncoding nucleotides that serve as intracellular and extracellular signaling molecules. A previous collaboration found miR-127/3p circulation in the blood of breast cancer patients correlated with improved patient recovery and prognosis. While this study exclusively focused on breast cancer patients, data mining of the TCGA databases indicated that miR-127/3p may be positively associated with outcomes in other cancer types. In our study, A549 lung adenocarcinoma cells were transfected with miR-127/3p using Cell Block protocols produced by the Cell Biology Education Consortium (CBEC). After transfection, cell migration (scratch/wound healing) assays were used to determine the role miR-127/3p plays in the tumor microenvironment. To mimic and test this environment, transfected cells were incubated in normal oxygen (normoxic) and low oxygen (hypoxic) environments. We found that miR-127/3p inhibited cell migration in both normal oxygen and hypoxic environments. These results help elucidate the role miR-127/3p plays in the prevention of metastasis and further highlight its potential as a positive biomarker.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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