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Loss of the vitellogenins confers a fitness disadvantage but does not impact brood size in C. elegans. 在秀丽隐杆线虫中，卵黄原蛋白的丧失会造成适合度的劣势，但不会影响后代的大小。
microPublication biology Pub Date : 2025-09-10 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001789
Monica M Macharios, Yasmine D Hernandez, Peter C Breen, Robert H Dowen
{"title":"Loss of the vitellogenins confers a fitness disadvantage but does not impact brood size in <i>C. elegans</i>.","authors":"Monica M Macharios, Yasmine D Hernandez, Peter C Breen, Robert H Dowen","doi":"10.17912/micropub.biology.001789","DOIUrl":"10.17912/micropub.biology.001789","url":null,"abstract":"<p><p>Organismal homeostasis relies on balancing cellular metabolic decisions with environmental conditions, especially during reproduction. Using <i>Caenorhabditis elegans</i> , we tested whether vitellogenesis, or the deposition of lipid-rich yolk into oocytes, is required for reproductive output and metabolic balance by creating a strain lacking all six vitellogenin genes ( <i>vit-1-6</i> ). This mutant produced embryos with reduced lipid content compared to wild-type, but the total brood size remained unaffected, unlike the <i>rme-2</i> mutant, which lacks the yolk receptor. However, progeny survival during L1 starvation was impaired in <i>vit-1-6</i> animals. This strain offers a new model for studying how vitellogenesis impacts reproductive and organismal fitness.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12461288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Catecholamines Differ in Their Capacity to Form Melanin. 儿茶酚胺形成黑色素的能力不同。
microPublication biology Pub Date : 2025-09-09 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001737
Lin Rayes, David Njus
{"title":"Catecholamines Differ in Their Capacity to Form Melanin.","authors":"Lin Rayes, David Njus","doi":"10.17912/micropub.biology.001737","DOIUrl":"10.17912/micropub.biology.001737","url":null,"abstract":"<p><p>Upon oxidation, the catechol amino acid L-DOPA polymerizes spontaneously to form the insoluble pigment melanin. Related catechols are less prone to polymerization, however, and this correlates with function. Comparison of the oxidation products of these catechols reveals that L-DOPA, dopamine, norepinephrine and epinephrine form a red-colored \"chrome\" upon oxidation, but epinephrine and norepinephrine, which act as neurohormones and neurotransmitters, do not go on to form an insoluble melanin. N-acetyldopamine (NADA), which functions in cuticle hardening in insects, does not even cyclize to the \"chrome,\" so the quinone remains available to crosslink cuticular proteins without obscuring coloration by other pigments. Thus, the side-chains of catecholamines affect reactivity as well as function.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elucidation of the 5' End of the Zebrafish col2a1b gene at the Telomeric-End of Chromosome 11. 斑马鱼11号染色体端粒末端col2a1b基因5'端的解析
microPublication biology Pub Date : 2025-09-09 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001729
Angelina R Carcione, Rodney M Dale
{"title":"Elucidation of the 5' End of the Zebrafish <i>col2a1b</i> gene at the Telomeric-End of Chromosome 11.","authors":"Angelina R Carcione, Rodney M Dale","doi":"10.17912/micropub.biology.001729","DOIUrl":"10.17912/micropub.biology.001729","url":null,"abstract":"<p><p>Type II collagens (Col2) play a vital role in the formation of many vertebrate structures, such as the skeleton, the notochord, and many other tissues. This study sets out to clarify the start of the genomic annotation around one of the zebrafish orthologs of vertebrate Type II collagen, <i>col2a1b ,</i> as it sits at the telomeric end of chromosome 11 and up till this point has missing coding and non-coding exons form the published databases. We have used bioinformatic tools to elucidate this missing genomic sequence and analyze for basal promoter elements that designate the true first exon of <i>col2a1b</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple protocol for producing axenic seeds of Sorghum bicolor. 高粱双色无性系种子的简易生产工艺。
microPublication biology Pub Date : 2025-09-09 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001772
Beatrice Bock, Jack Scherer, Faith Parrish, Julia Burnside, Callum Rohrer, Catherine Gehring
{"title":"A simple protocol for producing axenic seeds of <i>Sorghum bicolor</i>.","authors":"Beatrice Bock, Jack Scherer, Faith Parrish, Julia Burnside, Callum Rohrer, Catherine Gehring","doi":"10.17912/micropub.biology.001772","DOIUrl":"10.17912/micropub.biology.001772","url":null,"abstract":"<p><p>Microbes within seeds can confound research on microbial colonization, symbiosis, and pathogenesis. Sterilization of both external and internal seed tissues is therefore essential in certain experiments, but the method must also preserve seed viability. Here, we present a reliable and simple protocol for sterilizing <i>Sorghum bicolor</i> seeds by submerging them in 95% ethanol for 2 minutes followed by 3.75% sodium hypochlorite for 20 minutes. This approach yielded a low contamination rate (2 out of 95 seeds) and a robust median germination rate (63%). Its simplicity, cost-effectiveness, and accessibility make it a practical option for experiments requiring axenic seeds.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145139686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GelDrop Array High-Throughput Screening in Nematodes. GelDrop阵列在线虫中的高通量筛选
microPublication biology Pub Date : 2025-09-05 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001809
Xuan Wan, Paul W Sternberg
{"title":"GelDrop Array High-Throughput Screening in Nematodes.","authors":"Xuan Wan, Paul W Sternberg","doi":"10.17912/micropub.biology.001809","DOIUrl":"10.17912/micropub.biology.001809","url":null,"abstract":"<p><p>Nematode Growth Medium(NGM) plate-based screens consume substantial materials and time. We present GelDrop Array Screening (GelDrop), a gellan gum hydrogel droplet platform for <i>Caenorhabditis elegans</i> genetic crosses, mutation discovery, transgene integration, and small-molecule assays. Single animals are confined in discrete, bacteria ( OP50 )-supplemented droplets that sustain growth and reproduction for 2-3 days, providing screenable progeny while preventing escape and cross-contamination. Each 10-cm Petri dish supports 70-78 parallel screens. GelDrop can largely reduce agar use and labeling burden, boost throughput, and lower cost compared with conventional agar. The approach is simple, scalable, and compatible with standard microscopes and routine worm handling.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fibrillin-Related Proteins Control Calcium Homeostasis in Dystrophic Muscle Across Species. 纤维蛋白相关蛋白控制不同物种营养不良肌肉钙稳态。
microPublication biology Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001697
Damiano Marchiafava, Andres Vidal Gadea
{"title":"Fibrillin-Related Proteins Control Calcium Homeostasis in Dystrophic Muscle Across Species.","authors":"Damiano Marchiafava, Andres Vidal Gadea","doi":"10.17912/micropub.biology.001697","DOIUrl":"10.17912/micropub.biology.001697","url":null,"abstract":"<p><p>Duchenne muscular dystrophy (DMD) involves progressive muscle degeneration associated with impaired calcium homeostasis, particularly defective calcium clearance during muscle relaxation. However, the mechanisms linking extracellular matrix (ECM) integrity to calcium regulation remain unclear. We investigated whether MUA-3 , a <i>Caenorhabditis elegans</i> fibrillin-related ECM protein, contributes to calcium dysregulation in dystrophic muscle. Using fluorescent calcium imaging in transgenic worms expressing muscle-specific GCaMP2, we found that <i>mua-3</i> downregulation selectively elevated resting calcium levels in healthy muscle, phenocopying the dystrophic calcium signature. Critically, partial <i>mua-3</i> downregulation had no additional effect in dystrophic ( <i>dys-1</i> ) muscle, where <i>mua-3</i> expression was already reduced by 57%, suggesting loss of <i>mua-3</i> function contributes to dystrophic pathology. In human dystrophic myoblasts, we observed parallel findings: elevated sarcoplasmic calcium concurrent with significant downregulation of fibrillin genes <i>FBN1/FBN2</i> . These findings identify fibrillin-related proteins as potential regulators of muscle calcium homeostasis across species and suggest that ECM-calcium coupling represents a conserved pathological mechanism in muscular dystrophy.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum: DeleteROI for Cleaning CiliaQ Output of Non-ciliary Contamination. 勘误：DeleteROI清洁纤毛输出非纤毛污染。
microPublication biology Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001839
{"title":"Erratum: DeleteROI for Cleaning CiliaQ Output of Non-ciliary Contamination.","authors":"","doi":"10.17912/micropub.biology.001839","DOIUrl":"10.17912/micropub.biology.001839","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.17912/micropub.biology.001670.].</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CD320 expression is increased in breast cancer stem cells but does not promote their expansion or alter key histone methylation marks. CD320在乳腺癌干细胞中的表达增加，但不促进其扩增或改变关键组蛋白甲基化标记。
microPublication biology Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001696
Christine Carney, Lisa Jenkins, Connor Jewell, Rachel Carter, Puneet Mann, Binwu Tang, Senthil Muthuswamy, Lalage Wakefield
{"title":"CD320 expression is increased in breast cancer stem cells but does not promote their expansion or alter key histone methylation marks.","authors":"Christine Carney, Lisa Jenkins, Connor Jewell, Rachel Carter, Puneet Mann, Binwu Tang, Senthil Muthuswamy, Lalage Wakefield","doi":"10.17912/micropub.biology.001696","DOIUrl":"10.17912/micropub.biology.001696","url":null,"abstract":"<p><p>Several lines of evidence suggest a potential link between stemness and vitamin B12, which contributes to S-adenosylmethionine (SAM) generation and hence methylation-dependent epigenetic control. Here we found that the vitamin B12 receptor, CD320, is significantly increased in cancer stem cells (CSCs) in models of triple-negative breast cancer <i>.</i> We hypothesized that elevated CD320 expression promotes CSC expansive self-renewal via increased SAM-mediated histone H3K4 and H3K36 trimethylation, which were previously implicated in stemness and plasticity. However, we found that modulation of CD320 expression had no effect on SAM, H3K4me3 and H3K36me3 levels, or on CSC expansion <i>in vitro</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dissecting the impact of N-acetylmannosamine (ManNAc) on ganglioside levels in a sialin-deficient cell model. 剖析n-乙酰甘露糖胺（ManNAc）对唾液素缺乏细胞模型中神经节苷脂水平的影响。
microPublication biology Pub Date : 2025-09-02 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001733
Marya S Sabir, Marjan Huizing, William A Gahl, Frances M Platt, May Christine V Malicdan
{"title":"Dissecting the impact of N-acetylmannosamine (ManNAc) on ganglioside levels in a sialin-deficient cell model.","authors":"Marya S Sabir, Marjan Huizing, William A Gahl, Frances M Platt, May Christine V Malicdan","doi":"10.17912/micropub.biology.001733","DOIUrl":"10.17912/micropub.biology.001733","url":null,"abstract":"<p><p>Lysosomal free sialic acid storage disorder (FSASD) is an ultra-rare neurodegenerative condition caused by mutations in <i>SLC17A5</i> , which encodes the lysosomal sialic acid exporter, sialin. Deficiency of sialin leads to lysosomal accumulation of unconjugated (\"free\") sialic acid. This study investigated the ability of N-acetylmannosamine (ManNAc), a precursor of sialic acid, to rescue glycosphingolipid (GSL) sialylation in a SLC17A5-deficient HEK-293T model system. Our findings reveal that while ManNAc supplementation may enhance sialic acid biosynthesis, it does not fully restore ganglioside sialylation to wild-type levels, highlighting the essential role of lysosomal sialic acid recycling in maintaining GSL sialylation homeostasis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A single component landing site for efficient transgenesis using recombination-mediated insertion. 利用重组介导插入高效转基因的单组分着陆点。
microPublication biology Pub Date : 2025-09-02 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001784
Aiden A Beck, Michael L Nonet
{"title":"A single component landing site for efficient transgenesis using recombination-mediated insertion.","authors":"Aiden A Beck, Michael L Nonet","doi":"10.17912/micropub.biology.001784","DOIUrl":"10.17912/micropub.biology.001784","url":null,"abstract":"<p><p>Recombination-mediated insertion (RMI) is a recently developed method for creating <i>C. elegans</i> transgenes using phiC31-mediated recombination. RMI was developed as a two-component approach that relies on an <i>att</i> landing site and an unlinked source of phiC31 and FLP recombinases. Here, we describe both a landing site that incorporates the recombinase cassette and matching targeting vectors. The new landing site is located at a well-vetted MosSCI insertion on Chr IV and injections of single animals typically yield multiple independent insertions. We document the utility of this RMI landing site by creating 14 fluorescent reporters for pharyngeal gland cells.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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