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The lim-7p::ced-1::GFP transgene from the MD701 strain increases embryonic lethality in C. elegans.
microPublication biology Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001420
Nina M Zampetti, Kristen A Quaglia, Lisa N Petrella
{"title":"The <i>lim-7p::ced-1::GFP</i> transgene from the MD701 strain increases embryonic lethality in <i>C. elegans</i>.","authors":"Nina M Zampetti, Kristen A Quaglia, Lisa N Petrella","doi":"10.17912/micropub.biology.001420","DOIUrl":"10.17912/micropub.biology.001420","url":null,"abstract":"<p><p>The <i>lim-7p::ced-1::GFP</i> transgene has been widely used for evaluating germline apoptosis in <i>C. elegans</i> . Here we observed an increase in embryonic lethality in the MD701 strain that contains the <i>lim-7p::ced-1::GFP</i> transgene and a strain that outcrossed the <i>lim-7p::ced-1::GFP</i> transgene into the N2 wild-type strain. While the outcrossed strain had a significantly lower level of embryonic lethality than MD701, it still showed significantly higher embryonic lethality than wild type. Our results suggest that the presence of the <i>lim-7p::ced-1::GFP</i> transgene significantly increases embryonic lethality, but there may also be a secondary mutation in the MD701 strain that further increases embryonic lethality.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CEP44 and CCDC15 label the spermatozoa proximal and atypical distal centrioles.
microPublication biology Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001393
Luke Achinger, Briggs Hehl, Jason Suh, Samantha B Schon, Nagalakshmi Nadiminty, Tariq A Shah, Puneet Sindhwani, Tomer Avidor-Reiss
{"title":"CEP44 and CCDC15 label the spermatozoa proximal and atypical distal centrioles.","authors":"Luke Achinger, Briggs Hehl, Jason Suh, Samantha B Schon, Nagalakshmi Nadiminty, Tariq A Shah, Puneet Sindhwani, Tomer Avidor-Reiss","doi":"10.17912/micropub.biology.001393","DOIUrl":"10.17912/micropub.biology.001393","url":null,"abstract":"<p><p>The centrosome is a conserved characteristic of eukaryotic and human cells but is highly specialized in reproductive cells. The spermatozoan centrosome includes a slightly modified proximal centriole, an atypical distal centriole, and specialized pericentriolar material, including striated columns and capitellum. We investigated the localization of canonical centriolar proteins CEP44 and CCDC15 in human spermatozoa. We found that CEP44 localizes mainly at the proximal centriole and distal centriole bases relative to centrin. CCDC15 colocalizes with centrin in both the proximal centriole and distal centriole. These findings further our understanding of the spermatozoan centrosome composition.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene model for the ortholog of Glys in Drosophila simulans.
microPublication biology Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001168
Madeline L Gruys, Madison A Sharp, Zachary Lill, Caroline Xiong, Amy T Hark, James J Youngblom, Chinmay P Rele, Laura K Reed
{"title":"Gene model for the ortholog of <i>Glys</i> in <i>Drosophila simulans</i>.","authors":"Madeline L Gruys, Madison A Sharp, Zachary Lill, Caroline Xiong, Amy T Hark, James J Youngblom, Chinmay P Rele, Laura K Reed","doi":"10.17912/micropub.biology.001168","DOIUrl":"10.17912/micropub.biology.001168","url":null,"abstract":"<p><p>Gene model for the ortholog of glycogen synthase ( <i>Glys</i> ) in the <i>Drosophila simulans</i> May 2017 (Princeton ASM75419v2/DsimGB2) Genome Assembly (GenBank Accession: GCA_000754195.3 ). This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus <i>Drosophila</i> using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dopaminergic neurodegeneration in C. elegans cultivated with Porphorymonas gingivalis. 用牙龈卟啉单胞菌培养秀丽隐杆线虫的多巴胺能神经变性。
microPublication biology Pub Date : 2025-01-06 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001423
Edward F Griffin, Madeline G Owens
{"title":"Dopaminergic neurodegeneration in <i>C. elegans</i> cultivated with <i>Porphorymonas gingivalis</i>.","authors":"Edward F Griffin, Madeline G Owens","doi":"10.17912/micropub.biology.001423","DOIUrl":"10.17912/micropub.biology.001423","url":null,"abstract":"<p><p>Disruption of the human microbiome has emerged as a major contributing factor in the etiology of neurodegenerative disease. Previous work suggests a positive correlation between periodontal inflammation and Parkinson's disease. Here, we show that feeding <i>C. elegans</i> animals <i>Porphorymonas gingivalis</i> causes neurodegeneration that is not additive with neurodegeneration induced by the Parkinson's-associated protein, α-synuclein. In contrast, α-synuclein-expressing animals fed <i>P. gingivalis</i> show additional disruption in basal slowing, suggesting that <i>P. gingivalis</i> induces neurodegeneration while altering neuronal function of extant neurons. Though the mechanism is unclear, these results suggest a relationship between <i>P. gingivalis</i> and neurodegeneration that warrants further investigation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Voluntary intake of non-caloric sweetener drives conditioned bottle-position preference in mice. 自愿摄入无热量甜味剂驱动小鼠对瓶子位置的条件偏好。
microPublication biology Pub Date : 2025-01-06 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001459
Daisuke H Tanaka, Tsutomu Tanabe
{"title":"Voluntary intake of non-caloric sweetener drives conditioned bottle-position preference in mice.","authors":"Daisuke H Tanaka, Tsutomu Tanabe","doi":"10.17912/micropub.biology.001459","DOIUrl":"10.17912/micropub.biology.001459","url":null,"abstract":"<p><p>The present study investigated whether saccharin, a non-caloric sweetener, induces conditioned bottle-position preference in mice. In a two-bottle preference test, the mice initially preferred water from a specific side. When saccharin was introduced on the opposite side, the mice showed increased total intake and a preference for the position of the saccharin bottle. After saccharin removal, the preference for the saccharin-associated position persisted for one day but disappeared by the next day. These findings suggest that saccharin intake drives associative learning between its presence and bottle position, influencing subsequent decision-making and motivation to consume from the previously saccharin-associated position.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome Sequence of Three Siphoviruses in the EE, GA and EA5 Actinobacteriophage Clusters: Biscayne, Bush and GreenIvy. Biscayne， Bush和GreenIvy放线菌噬菌体群EE， GA和EA5中三种siphovirus的基因组序列
microPublication biology Pub Date : 2025-01-06 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001397
Xavier S Purroy, Betty R Sierra, Lara Becerra Reymundo, Victoria M Serradet, Alejandra M Camacho, Nicole A Briceno, Katherine Artiles, Pooja Lad, Nhan Phan, Alison Rodriguez Leiva, Jazlyn N Appolon, Akram Mikhail, Arianna M Ruiz, Carlos Rodriguez, David Vega, Gabriela Moyano, Grace Intrator, Kiryl Yasinski, Kristen Mclean, Nicole Gonzalez Giliberti, Erika Ramirez Ramirez, Victor Adolpho de Melo, Alexandra S Alsina, Maria Y Andino, Brian A Becker, Hillary Castellanos, Natalia A Castillo, Brandon S Fernandez, Jeremiah R Estinvil, Amanda A Gonzalez, Emily M Hernandez, Ayden Ho, Sheikh F Islam, Anna Liubenco, Lance Mejia, Sandra N Meesala, William Morales-Ramirez, Nathalie Morlote, Kevin Ramos-Homs, Jorge A Rodriguez, Leydis M Torres, Patricia Waikel, Jaime Mayoral
{"title":"Genome Sequence of Three Siphoviruses in the EE, GA and EA5 Actinobacteriophage Clusters: Biscayne, Bush and GreenIvy.","authors":"Xavier S Purroy, Betty R Sierra, Lara Becerra Reymundo, Victoria M Serradet, Alejandra M Camacho, Nicole A Briceno, Katherine Artiles, Pooja Lad, Nhan Phan, Alison Rodriguez Leiva, Jazlyn N Appolon, Akram Mikhail, Arianna M Ruiz, Carlos Rodriguez, David Vega, Gabriela Moyano, Grace Intrator, Kiryl Yasinski, Kristen Mclean, Nicole Gonzalez Giliberti, Erika Ramirez Ramirez, Victor Adolpho de Melo, Alexandra S Alsina, Maria Y Andino, Brian A Becker, Hillary Castellanos, Natalia A Castillo, Brandon S Fernandez, Jeremiah R Estinvil, Amanda A Gonzalez, Emily M Hernandez, Ayden Ho, Sheikh F Islam, Anna Liubenco, Lance Mejia, Sandra N Meesala, William Morales-Ramirez, Nathalie Morlote, Kevin Ramos-Homs, Jorge A Rodriguez, Leydis M Torres, Patricia Waikel, Jaime Mayoral","doi":"10.17912/micropub.biology.001397","DOIUrl":"10.17912/micropub.biology.001397","url":null,"abstract":"<p><p>Bacteriophages Biscayne, Bush and GreenIvy were isolated from soil samples in Miami, FL using <i>Microbacterium foliorum</i> NRRL B-24224 as host. Transmission electron microscopy shows siphoviral morphologies for all three phages. Based on gene content similarity to other actinobacteriophages, they are assigned to the EE, GA and EA5 clusters, respectively.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome sequence of WestPM, a phage infecting Microbacterium foliorum isolated from beach environmental samples. 海滩环境样品中感染叶状微杆菌的噬菌体WestPM的基因组序列。
microPublication biology Pub Date : 2025-01-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001395
Charles J West, Brittany C Yencho, Andrew J Brown, Conor R Flannigan, Hui-Min Chung
{"title":"Genome sequence of WestPM, a phage infecting <i>Microbacterium foliorum</i> isolated from beach environmental samples.","authors":"Charles J West, Brittany C Yencho, Andrew J Brown, Conor R Flannigan, Hui-Min Chung","doi":"10.17912/micropub.biology.001395","DOIUrl":"10.17912/micropub.biology.001395","url":null,"abstract":"<p><p>Bacteriophage WestPM is a siphoviral-like phage infecting <i>Microbacterium foliorum</i> isolated from environmental samples collected on Pensacola Beach, FL. The genome of this phage is 39,693 bp long and contains 59 predicted protein-coding genes and zero tRNA genes. Based on gene content similarity, WestPM is grouped in the actinobacteriophage EA11 subcluster.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete Genome Sequence of the Cluster DJ Actinobacteriophage, Petito, isolated on the host Gordonia rubripertinca. 宿主红带戈登菌（Gordonia rubripertinca）上分离的DJ簇放线菌噬菌体Petito的全基因组序列
microPublication biology Pub Date : 2025-01-03 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001456
Elliot Benson, Micah Blount, Shria Chauhan, Jayden Ehrhart, Adelinn Foster, Abigail Ingber, Madeline Julian, Derika Kwansah, Trang Le, Emily May, Elizabeth Mazel, Esther Morency, Sierra Nelson, Casey O'Toole, Kaitlin Potter, Leandra Vita, Kirra Weigand, Denise Monti
{"title":"Complete Genome Sequence of the Cluster DJ Actinobacteriophage, Petito, isolated on the host <i>Gordonia rubripertinca</i>.","authors":"Elliot Benson, Micah Blount, Shria Chauhan, Jayden Ehrhart, Adelinn Foster, Abigail Ingber, Madeline Julian, Derika Kwansah, Trang Le, Emily May, Elizabeth Mazel, Esther Morency, Sierra Nelson, Casey O'Toole, Kaitlin Potter, Leandra Vita, Kirra Weigand, Denise Monti","doi":"10.17912/micropub.biology.001456","DOIUrl":"10.17912/micropub.biology.001456","url":null,"abstract":"<p><p>Gordonia phage Petito is a newly discovered siphovirus that infects <i>Gordonia rubripertincta</i> NRRL B-16540. The double-stranded DNA genome of this phage is 60,447 bp long with 93 predicted protein-coding genes and no tRNAs. Petito is a Cluster DJ phage.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biochemical characterization of collagen I in Warmblood Fragile Foal Syndrome horse lysyl hydroxylase 1 mutation. 温血脆弱马驹综合征马赖氨酸羟化酶1突变中I型胶原蛋白的生化特征。
microPublication biology Pub Date : 2025-01-03 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001399
Yoshihiro Ishikawa, Sara F Tufa, Douglas R Keene, Hans Peter Bächinger, Nena J Winand
{"title":"Biochemical characterization of collagen I in Warmblood Fragile Foal Syndrome horse lysyl hydroxylase 1 mutation.","authors":"Yoshihiro Ishikawa, Sara F Tufa, Douglas R Keene, Hans Peter Bächinger, Nena J Winand","doi":"10.17912/micropub.biology.001399","DOIUrl":"10.17912/micropub.biology.001399","url":null,"abstract":"<p><p>Mutations in the collagen-modifying enzyme lysyl hydroxylase 1 (LH1) cause Warmblood Fragile Foal Syndrome (WFFS) in horses. We investigated the impact of this mutation on collagen structure and function. Our results show that LH1 deficiency leads to reduced lysine hydroxylation, altered collagen fibril organization, and tissue abnormalities resembling human Ehlers-Danlos syndrome. These findings highlight the critical role of LH1 in collagen biosynthesis and provide insights into the pathogenesis of WFFS.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved RNA toehold switch sensitivity using the NanoLuc complementation reporter. 利用NanoLuc互补报告基因提高RNA支点开关灵敏度。
microPublication biology Pub Date : 2025-01-02 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001334
Katherinne J Diaz, Julian Jarquin, Aleksandr Petrosyan, Melissa K Takahashi
{"title":"Improved RNA toehold switch sensitivity using the NanoLuc complementation reporter.","authors":"Katherinne J Diaz, Julian Jarquin, Aleksandr Petrosyan, Melissa K Takahashi","doi":"10.17912/micropub.biology.001334","DOIUrl":"10.17912/micropub.biology.001334","url":null,"abstract":"<p><p>RNA toehold switches are powerful tools that can be used as biosensors to detect nearly any RNA sequence. In the presence of a specific RNA trigger, the toehold switch allows translation of a reporter protein. Toehold switches expressed in cell-free expression systems have been used as biosensors for several viruses and bacterial RNAs. Here we explore the use of the NanoLuc complementation reporter NanoBiT and found that it provides improved sensitivity over sfGFP. This reporter can be applied broadly across other RNA toehold switch applications.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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