{"title":"CD320在乳腺癌干细胞中的表达增加，但不促进其扩增或改变关键组蛋白甲基化标记。","authors":"Christine Carney, Lisa Jenkins, Connor Jewell, Rachel Carter, Puneet Mann, Binwu Tang, Senthil Muthuswamy, Lalage Wakefield","doi":"10.17912/micropub.biology.001696","DOIUrl":null,"url":null,"abstract":"<p><p>Several lines of evidence suggest a potential link between stemness and vitamin B12, which contributes to S-adenosylmethionine (SAM) generation and hence methylation-dependent epigenetic control. Here we found that the vitamin B12 receptor, CD320, is significantly increased in cancer stem cells (CSCs) in models of triple-negative breast cancer <i>.</i> We hypothesized that elevated CD320 expression promotes CSC expansive self-renewal via increased SAM-mediated histone H3K4 and H3K36 trimethylation, which were previously implicated in stemness and plasticity. However, we found that modulation of CD320 expression had no effect on SAM, H3K4me3 and H3K36me3 levels, or on CSC expansion <i>in vitro</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450303/pdf/","citationCount":"0","resultStr":"{\"title\":\"CD320 expression is increased in breast cancer stem cells but does not promote their expansion or alter key histone methylation marks.\",\"authors\":\"Christine Carney, Lisa Jenkins, Connor Jewell, Rachel Carter, Puneet Mann, Binwu Tang, Senthil Muthuswamy, Lalage Wakefield\",\"doi\":\"10.17912/micropub.biology.001696\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Several lines of evidence suggest a potential link between stemness and vitamin B12, which contributes to S-adenosylmethionine (SAM) generation and hence methylation-dependent epigenetic control. Here we found that the vitamin B12 receptor, CD320, is significantly increased in cancer stem cells (CSCs) in models of triple-negative breast cancer <i>.</i> We hypothesized that elevated CD320 expression promotes CSC expansive self-renewal via increased SAM-mediated histone H3K4 and H3K36 trimethylation, which were previously implicated in stemness and plasticity. However, we found that modulation of CD320 expression had no effect on SAM, H3K4me3 and H3K36me3 levels, or on CSC expansion <i>in vitro</i> .</p>\",\"PeriodicalId\":74192,\"journal\":{\"name\":\"microPublication biology\",\"volume\":\"2025 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450303/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"microPublication biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17912/micropub.biology.001696\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001696","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
CD320 expression is increased in breast cancer stem cells but does not promote their expansion or alter key histone methylation marks.
Several lines of evidence suggest a potential link between stemness and vitamin B12, which contributes to S-adenosylmethionine (SAM) generation and hence methylation-dependent epigenetic control. Here we found that the vitamin B12 receptor, CD320, is significantly increased in cancer stem cells (CSCs) in models of triple-negative breast cancer . We hypothesized that elevated CD320 expression promotes CSC expansive self-renewal via increased SAM-mediated histone H3K4 and H3K36 trimethylation, which were previously implicated in stemness and plasticity. However, we found that modulation of CD320 expression had no effect on SAM, H3K4me3 and H3K36me3 levels, or on CSC expansion in vitro .
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