A single component landing site for efficient transgenesis using recombination-mediated insertion.

Abstract

Recombination-mediated insertion (RMI) is a recently developed method for creating C. elegans transgenes using phiC31-mediated recombination. RMI was developed as a two-component approach that relies on an att landing site and an unlinked source of phiC31 and FLP recombinases. Here, we describe both a landing site that incorporates the recombinase cassette and matching targeting vectors. The new landing site is located at a well-vetted MosSCI insertion on Chr IV and injections of single animals typically yield multiple independent insertions. We document the utility of this RMI landing site by creating 14 fluorescent reporters for pharyngeal gland cells.