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Characterization and comparison of Schizosaccharomyces pombe cdc15 temperature-sensitive mutants. Schizosaccharomyces pombe cdc15温度敏感突变体的鉴定与比较。
microPublication biology Pub Date : 2025-03-05 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001515
Lesley A Turner, Aleksandar Vjestica, Alaina H Willet, Snezhana Oliferenko, Kathleen L Gould
{"title":"Characterization and comparison of <i>Schizosaccharomyces pombe</i> <i>cdc15</i> temperature-sensitive mutants.","authors":"Lesley A Turner, Aleksandar Vjestica, Alaina H Willet, Snezhana Oliferenko, Kathleen L Gould","doi":"10.17912/micropub.biology.001515","DOIUrl":"10.17912/micropub.biology.001515","url":null,"abstract":"<p><p>The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast <i>Schizosaccharomyces pombe</i> , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared <i>cdc15</i> temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each <i>cdc15</i> mutant allele and analyzed their growth at different temperatures. Additionally, we report a new <i>cdc15</i> allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in <i>S. pombe</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene model for the ortholog of Pdk1 in Drosophila grimshawi. 果蝇Pdk1同源基因的基因模型研究。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001042
Megan E Lawson, Madison A Sharp, Laura K Reed, Norma Velazquez-Ulloa, Amy T Hark, John Stanga, Chinmay P Rele
{"title":"Gene model for the ortholog of <i>Pdk1</i> in <i>Drosophila grimshawi</i>.","authors":"Megan E Lawson, Madison A Sharp, Laura K Reed, Norma Velazquez-Ulloa, Amy T Hark, John Stanga, Chinmay P Rele","doi":"10.17912/micropub.biology.001042","DOIUrl":"10.17912/micropub.biology.001042","url":null,"abstract":"<p><p>Gene model for the ortholog of Phosphoinositide-dependent kinase 1 ( <i>Pdk1</i> ) in the May 2011 (Agencourt dgri_caf1/DgriCAF1) Genome Assembly (GenBank Accession: GCA_000005155.1 ) of <i>Drosophila grimshawi</i> . This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus <i>Drosophila</i> using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome Sequences of the Arthrobacter globiformis Phage BillyTP (Cluster AY) and Gordonia rubripertincta Phage MAnor (Cluster CT). 球形节杆菌噬菌体BillyTP （Cluster AY）和红丛戈登菌噬菌体MAnor （Cluster CT）的基因组序列。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001486
James Melton, Camilla Augustus, Jordan Dotson, Adja Camara, Laila Christian, Damera McClendon, McKenzie Severson, Morgan Wills, Deana Burris, Destinee Williams, Vashti Williams, Shrijeeta Ganguly
{"title":"Genome Sequences of the <i>Arthrobacter globiformis</i> Phage BillyTP (Cluster AY) and <i>Gordonia rubripertincta</i> Phage MAnor (Cluster CT).","authors":"James Melton, Camilla Augustus, Jordan Dotson, Adja Camara, Laila Christian, Damera McClendon, McKenzie Severson, Morgan Wills, Deana Burris, Destinee Williams, Vashti Williams, Shrijeeta Ganguly","doi":"10.17912/micropub.biology.001486","DOIUrl":"10.17912/micropub.biology.001486","url":null,"abstract":"<p><p>Phages BillyTP (Cluster AY) and MAnor (Cluster CT) were isolated from soil using <i>Arthrobacter globiformis</i> B-2979 and <i>Gordonia rubripertincta</i> NRRL B-16540, respectively, as hosts. The genome of BillyTP is 53,003 base pairs (bp) and contains 96 putative genes, while the genome of MAnor is 48,333 bp encoding 73 putative genes. BillyTP and MAnor are assigned to actinobacteriophage clusters AY and CT, respectively, based on gene content similarity (GCS) of at least 35%.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene model for the ortholog of Roc1a in Drosophila eugracilis. 果蝇Roc1a同源基因的基因模型研究。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001028
Megan E Lawson, Isabel G Wellik, Bridgiet Alvarado, Tanner German, Jeffrey S Thompson, Lindsey J Long, Justin R DiAngelo, Melinda A Yang, Chinmay P Rele, Laura K Reed
{"title":"Gene model for the ortholog of <i>Roc1a</i> in <i>Drosophila eugracilis</i>.","authors":"Megan E Lawson, Isabel G Wellik, Bridgiet Alvarado, Tanner German, Jeffrey S Thompson, Lindsey J Long, Justin R DiAngelo, Melinda A Yang, Chinmay P Rele, Laura K Reed","doi":"10.17912/micropub.biology.001028","DOIUrl":"10.17912/micropub.biology.001028","url":null,"abstract":"<p><p>Gene model for the ortholog of <i>Regulator of cullins 1a</i> ( <i>Roc1a</i> ) in the <i>Drosophila eugracilis</i> Apr. 2013 (BCM-HGSC/Deug_2.0, DeugGB2) Genome Assembly (GenBank Accession: GCA_000236325.2). This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus <i>Drosophila</i> using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Consensus furin cleavage sites in the cuticular collagens DPY-17 and SQT-3 are required for Q neuroblast left-right asymmetric migration in Caenorhabditis elegans. 在秀丽隐杆线虫中，Q神经母细胞左右不对称迁移需要在表皮胶原DPY-17和SQT-3中达成一致的蛋白切割位点。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001526
Vedant D Jain, Celeste J Gormly, Erik A Lundquist
{"title":"Consensus furin cleavage sites in the cuticular collagens DPY-17 and SQT-3 are required for Q neuroblast left-right asymmetric migration in <i>Caenorhabditis elegans</i>.","authors":"Vedant D Jain, Celeste J Gormly, Erik A Lundquist","doi":"10.17912/micropub.biology.001526","DOIUrl":"10.17912/micropub.biology.001526","url":null,"abstract":"<p><p>Previous studies showed that the apically secreted cuticular collagens DPY-17 , SQT-3 , and DPY-14 control the left-right asymmetric migration of the Q neuroblasts in <i>Caenorhabditis. elegans</i> . Furthermore, apical secretion of DPY-17 and SQT-3 require the BLI-4 proprotein convertase of the subtilisin/kexin family and the consensus furin cleavage site (CFCS) in the N-terminus of DPY-17 and SQT-3 . Work here shows that the CFCS sites of DPY-17 and SQT-3 are required for their roles in Q neuroblast migration. <i>bli-4</i> mutants had only weak effects on Q neuroblast migration, possibly due to redundancy among isoforms. These results suggest that apical secretion of cuticular collagens is required for Q neuroblast migration. These collagens might themselves provide left-right asymmetric guidance information, or might regulate another aspect of Q cell interaction with the cuticle, such as adhesion.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteasome condensates repeatedly "contact and release" at the nuclear periphery during dissolution. 在溶解过程中，蛋白酶体在核周围反复“接触和释放”。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001553
Conner Butcher, Kyle VanderVen, Jianhui Li
{"title":"Proteasome condensates repeatedly \"contact and release\" at the nuclear periphery during dissolution.","authors":"Conner Butcher, Kyle VanderVen, Jianhui Li","doi":"10.17912/micropub.biology.001553","DOIUrl":"10.17912/micropub.biology.001553","url":null,"abstract":"<p><p>Stress-induced proteasome condensates have been identified in both yeast and mammalian cells. The biochemical properties and dynamics of proteasome condensates mainly depend on the specific stress conditions. In the budding yeast <i>Saccharomyces cerevisiae</i> , cytoplasmic proteasome condensates assemble from the nuclear proteasomes under glucose starvation conditions. Proteasome condensates rapidly dissipate, and proteasomes reimport to the nucleus within minutes upon glucose recovery. We characterize the kinetics and dynamics of proteasome condensates after glucose recovery. Proteasome condensates transiently associate nuclear membranes with a repetitive \"contact and release\" movement during dissolution. Our study provides new insight into the events leading to biomolecular condensate dissolution.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Air-liquid interface culture for live analysis of zebrafish vertebral growth. 气液界面培养用于斑马鱼椎体生长的活体分析。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001199
Shin Sato, Misaki Sakashita, Ryo Kagami, Naoyuki Wada
{"title":"Air-liquid interface culture for live analysis of zebrafish vertebral growth.","authors":"Shin Sato, Misaki Sakashita, Ryo Kagami, Naoyuki Wada","doi":"10.17912/micropub.biology.001199","DOIUrl":"10.17912/micropub.biology.001199","url":null,"abstract":"<p><p>Fish vertebrae change their shapes from simple cylinders to complex amphicoelous hourglass-like forms with lateral ridges as they grow. This growth process is difficult to observe <i>in vivo</i> due to the thickening of the muscles surrounding the vertebrae. In this study, we applied air-liquid interface culture to isolated zebrafish vertebrae to observe vertebral growth <i>in vitro</i> . The mineralization patterns of the cultured vertebrae were similar to those of <i>in vivo</i> growth, reproducing vertebral growth. This study is the first report to ensure live analysis of fish vertebral growth from late larval stages and the related cell behavior.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pollinators do not avoid or prefer a locally rare pollen colour morph in Erythronium americanum (Liliaceae). 传粉者不避免或更喜欢当地罕见的花粉颜色形态在美洲赤藓（百合科）。
microPublication biology Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001286
Jada A Ripley, Gwenyth A Deadman-Wylie, Amanda L Traylor, Emily J Austen
{"title":"Pollinators do not avoid or prefer a locally rare pollen colour morph in <i>Erythronium americanum</i> (Liliaceae).","authors":"Jada A Ripley, Gwenyth A Deadman-Wylie, Amanda L Traylor, Emily J Austen","doi":"10.17912/micropub.biology.001286","DOIUrl":"10.17912/micropub.biology.001286","url":null,"abstract":"<p><p>The red-yellow pollen colour polymorphism that occurs in populations of <i>Erythronium americanum</i> provides an excellent opportunity to investigate the conditions leading to maintenance of phenotypic variation. Using arrays of cut <i>E. americanum</i> flowers with red- or yellow-pollen set in a naturally monomorphic red-pollen population, we tested whether pollinators exhibit frequency-dependent behaviours <i>.</i> As was previously observed when pollinator preference was tested in a pollen-colour polymorphic population, pollinators accustomed to the monomorphic red-pollen population in this study exhibited no pollen colour preference among flowers cut from a polymorphic population. The lack of pollinator colour preference against either a polymorphic or red-monomorphic background suggests that pollinators do not impose frequency-dependent selection. It is possible, however, that results would differ against a monomorphic yellow-pollen background.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Comparative Analysis of Transfected and Integrated Auxin Reporter Systems Reveals Sensitivity Advantages in Protoplast Transient Expression Assays. 生长素报告系统在原生质体瞬时表达分析中的敏感性优势
microPublication biology Pub Date : 2025-02-28 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001481
Joseph S Taylor, Eric A Villaseñor, James Rashkovsky, Jaime Simson, R Clay Wright, Bastiaan O R Bargmann
{"title":"A Comparative Analysis of Transfected and Integrated Auxin Reporter Systems Reveals Sensitivity Advantages in Protoplast Transient Expression Assays.","authors":"Joseph S Taylor, Eric A Villaseñor, James Rashkovsky, Jaime Simson, R Clay Wright, Bastiaan O R Bargmann","doi":"10.17912/micropub.biology.001481","DOIUrl":"https://doi.org/10.17912/micropub.biology.001481","url":null,"abstract":"<p><p>Reporter-gene activation studies using transient transformation of protoplasts are a powerful tool for the investigation of transcriptional regulation in plants. Here, we perform a comparative analysis of reporter-gene activation sensitivity using an integrated versus a co-transfected reporter-gene construct in Arabidopsis seedling mesophyll protoplasts. The DR5 synthetic auxin-responsive promoter was used to assay the response to auxin treatment and over-expression of activator Auxin Response Factors. We show that sensitivity, as measured by the fold-change in fluorescent-protein reporter-gene expression, is significantly increased by using a co-transfected reporter-gene construct.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective benefit of the sucrose TonB-dependent receptor, SucA, in Caulobacter crescentus. 蔗糖tonb依赖性受体SucA在新月形茎杆菌中的选择效益。
microPublication biology Pub Date : 2025-02-28 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001457
Erin NewRingeisen, Jacy Jordahl, Lisa Bowers
{"title":"Selective benefit of the sucrose TonB-dependent receptor, SucA, in <i>Caulobacter crescentus</i>.","authors":"Erin NewRingeisen, Jacy Jordahl, Lisa Bowers","doi":"10.17912/micropub.biology.001457","DOIUrl":"https://doi.org/10.17912/micropub.biology.001457","url":null,"abstract":"<p><p>Gram-negative bacteria have outer membrane proteins called TonB-dependent receptors (TBDRs) that facilitate energy-dependent transport of substrates. <i>Caulobacter crescentus</i> is a gram-negative bacterium with a large set of TBDRs, yet the function of many of these TBDRs remains uncharacterized. This study focuses on SucA, a TBDR that transports sucrose. Previous studies showed that <i>sucA</i> expression was induced in the presence of sucrose, yet did not provide a measurable fitness advantage under the conditions tested. This work identifies conditions where <i>sucA</i> does confer a significant growth advantage and provides evidence that SucA activity relies on the proton motive force, a feature of canonical TBDRs.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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