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Investigation of Conserved Amino Acids in the EGL-15 5A Domain Required for Sex Myoblast Migration in C. elegans. 对 elegans 中性肌细胞迁移所需的 EGL-15 5A 结构域中保守氨基酸的研究
microPublication biology Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001374
Birsen Gürkaynak, Dallas R Fonseca, Isabella Suarez, Michael J Stern, Te-Wen Lo
{"title":"Investigation of Conserved Amino Acids in the EGL-15 5A Domain Required for Sex Myoblast Migration in <i>C. elegans</i>.","authors":"Birsen Gürkaynak, Dallas R Fonseca, Isabella Suarez, Michael J Stern, Te-Wen Lo","doi":"10.17912/micropub.biology.001374","DOIUrl":"https://doi.org/10.17912/micropub.biology.001374","url":null,"abstract":"<p><p>Fibroblast Growth Factor Receptors (FGFRs) play a role in diverse developmental pathways that mediate cell proliferation, cell migration, and cell survival. We use <i>C. elegans</i> as a model to better understand FGFR signaling specificity. <i>C. elegans</i> has a single FGFR, EGL-15 . EGL-15 contains an alternatively spliced exon 5 that results in two isoforms, EGL-15(5A) and EGL-15(5B), that differ in their extracellular domains. The EGL-15(5A) isoform is required for the chemoattraction of a pair of migrating sex myoblasts, whose correct positioning is required for egg laying. Deletion of the 5A domain results in an egg-laying defective (Egl) organism. We identified six highly conserved amino acids within the 5A domain and found that two amino acids, S145 and L148 are specifically required for sex myoblast migration in <i>C. elegans</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ALG-1, a microRNA argonaute, promotes vulva induction in C. elegans. ALG-1是一种microRNA凋亡因子，可促进秀丽隐杆线虫的外阴诱导。
microPublication biology Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001373
Sunny Zhen, Christian E Rocheleau
{"title":"ALG-1, a microRNA argonaute, promotes vulva induction in <i>C. elegans</i>.","authors":"Sunny Zhen, Christian E Rocheleau","doi":"10.17912/micropub.biology.001373","DOIUrl":"10.17912/micropub.biology.001373","url":null,"abstract":"<p><p>Signaling by the LET-60 Ras GTPase/ MPK-1 Extracellular Regulated Kinase pathway specifies the vulva cell fate in <i>C. elegans</i> . The <i>let-7</i> miRNA family negatively regulates LET-60 Ras but other miRNAs can also modulate vulva induction. To determine the impact of globally reducing miRNA function on LET-60 Ras-mediated vulva induction we analyzed the effect of loss of the ALG-1 miRNA regulator on vulva development <i>.</i> Contrary to our expectations, we find that ALG-1 promotes vulva induction independently of LET-60 Ras. We found that the reduced vulva cell fate induction of <i>alg-1</i> deletion mutants could be due to delayed development of the vulva, or a requirement to maintain the competence of the uninduced precursor cells.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PALS-14 promotes resistance to Nematocida parisii infection in Caenorhabditis elegans. PALS-14能增强草履虫对拟线虫感染的抵抗力。
microPublication biology Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001325
Deevya Raman, Nicole Wernet, Spencer Gang, Emily Troemel
{"title":"PALS-14 promotes resistance to <i>Nematocida parisii</i> infection in <i>Caenorhabditis elegans</i>.","authors":"Deevya Raman, Nicole Wernet, Spencer Gang, Emily Troemel","doi":"10.17912/micropub.biology.001325","DOIUrl":"10.17912/micropub.biology.001325","url":null,"abstract":"<p><p>Microsporidia are common natural pathogens of the nematode <i>Caenorhabditis elegans</i> . Infection of <i>C. elegans</i> by the microsporidian species <i>Nematocida parisii</i> leads to induction of the Intracellular Pathogen Response (IPR), including transcriptional upregulation of 26 <i>pals</i> genes. The divergent ' <i>pals</i> ' sequence signature is conserved with humans, but PALS proteins have unknown biochemical functions. So far, none of the 26 induced <i>pals</i> genes have a demonstrated role in immunity. Here, we use RNAseq data, RNA interference, and CRISPR/Cas9 mutant analysis to identify the <i>N. parisii</i> -induced <i>pals-14</i> gene as an immune gene that provides defense against microsporidia infection in <i>C. elegans</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence for an interaction of paracingulin with microtubules. 副角蛋白与微管相互作用的证据
microPublication biology Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001341
Arielle Flinois, Annick Mutero-Maeda, Sylvie Montessuit, Sandra Citi
{"title":"Evidence for an interaction of paracingulin with microtubules.","authors":"Arielle Flinois, Annick Mutero-Maeda, Sylvie Montessuit, Sandra Citi","doi":"10.17912/micropub.biology.001341","DOIUrl":"10.17912/micropub.biology.001341","url":null,"abstract":"<p><p>The mechanisms that anchor microtubules to epithelial junctions are poorly understood. Here we show that recombinant purified paracingulin ( CGNL1 , JACOP), a cytoplasmic junctional protein, decorates microtubules by negative staining electron microscopy and co-pellets with microtubules. Co-pelleting experiments using fragments of CGNL1 indicate that this is mediated by a central region of the CGNL1 head domain (residues 250-420). Deletion of a basic amino-acid stretch (365-377) within this fragment, abolishes both co-pelleting with and decoration of microtubules. These results suggest that paracingulin can interact directly with microtubules through a basic amino-acid stretch of its head domain.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polo-like kinase inhibition leads to neuroprotection of neurons bearing alpha-synuclein Lewy body-like inclusions in vivo. Polo-like激酶抑制可对体内带有α-突触核蛋白路易体样包涵体的神经元起到神经保护作用。
microPublication biology Pub Date : 2024-10-10 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001348
Elizabeth P Rose, Jovin S Banga, Vivek K Unni
{"title":"Polo-like kinase inhibition leads to neuroprotection of neurons bearing alpha-synuclein Lewy body-like inclusions <i>in vivo</i>.","authors":"Elizabeth P Rose, Jovin S Banga, Vivek K Unni","doi":"10.17912/micropub.biology.001348","DOIUrl":"10.17912/micropub.biology.001348","url":null,"abstract":"<p><p>α-synuclein (αSyn) and S129 phosphorylated αSyn (pSyn) define synucleinopathies like Parkinson's disease (PD). Targeting S129 αSyn kinases, like the Polo-like kinase (PLK) family, could provide a therapeutic strategy to limit degeneration of cells bearing aggregated αSyn inclusions. Using longitudinal <i>in vivo</i> multiphoton imaging in mouse cortex after αSyn inclusion induction, we find an increase in cell survival of inclusion-bearing neurons after PLK inhibition. PLK inhibition is associated with increased αSyn levels within inclusions and increased nuclear DNA damage repair markers. Overall, these findings suggest that PLK inhibition may serve as a potential therapeutic strategy for limiting neurodegeneration in PD.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11507930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular and genetic characterization of Cbx-Basel , a new dominant allele of Ultrabithorax in D. melanogaster. Cbx-Basel的分子和遗传特征，它是黑腹蝇蛆Ultrabithorax的一个新的显性等位基因。
microPublication biology Pub Date : 2024-10-09 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001321
Basil Willi, Lukas Brügger, Leandra Müller, Stanley Tabor, Welcome Bender, Martin Müller
{"title":"Molecular and genetic characterization of <i>Cbx-Basel</i> , a new dominant allele of <i>Ultrabithorax</i> in D. melanogaster.","authors":"Basil Willi, Lukas Brügger, Leandra Müller, Stanley Tabor, Welcome Bender, Martin Müller","doi":"10.17912/micropub.biology.001321","DOIUrl":"https://doi.org/10.17912/micropub.biology.001321","url":null,"abstract":"<p><p>Dominant gain-of-function alleles for the homeotic gene <i>Ultrabithorax</i> ( <i>Ubx</i> ) have been known for a long time. They are summarized under the name <i>Contrabithorax</i> ( <i>Cbx</i> ). Such alleles are rather easy to spot because the morphology of the conspicuous dorsal wing appendage is often dramatically changed. The majority of these alleles is associated with chromosomal rearrangements that alter the genetic landscape of the <i>Ultrabithorax</i> locus. Thereby, UBX protein is ectopically expressed in the wing primordium where it is normally absent. Since <i>Ubx</i> specifies haltere identity, wing cells expressing UBX are determined to become haltere cells. However, apart from the prototypic allele <i>Cbx-1</i> , information on the molecular details of <i>Contrabithorax</i> alleles is scarce. Here, we present a rather detailed account on a novel Cbx-1-like allele called <i>Cbx-Basel</i> . The results of our study corroborate the model that has been postulated for the <i>Cbx-1</i> wing phenotype.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Saccades and pivoting during positive mechanotaxis in larval zebrafish. 斑马鱼幼体在正向机械轴向运动过程中的慢动作和转动。
microPublication biology Pub Date : 2024-10-09 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001349
Georgie Davies, Rodrigo De Marco
{"title":"Saccades and pivoting during positive mechanotaxis in larval zebrafish.","authors":"Georgie Davies, Rodrigo De Marco","doi":"10.17912/micropub.biology.001349","DOIUrl":"https://doi.org/10.17912/micropub.biology.001349","url":null,"abstract":"<p><p>Many animals are drawn to prominent sensory cues. Larval zebrafish consistently show attraction to minute water motions (mWMs). This positive mechanotaxis involves scanning-like eye movements (EMs) and changes in body orientation (pivoting), which likely enhance sensory detection. Video analysis shows that EMs increase during mWMs and negatively correlate with locomotion. Both the strength of mWMs and rearing conditions influence EM frequency, with alterations occurring after mWM offset. Additionally, EMs often accompany pivoting. The quantitative data presented here will aid in further exploring the neural bases of visual responses and positive mechanotaxis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reciprocal signaling between quorum sensing mutants: A model for division of labor. 法定人数感应突变体之间的相互信号传递：分工合作模型
microPublication biology Pub Date : 2024-10-08 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001326
Farah Abdul-Rahman, Joao Xavier
{"title":"Reciprocal signaling between quorum sensing mutants: A model for division of labor.","authors":"Farah Abdul-Rahman, Joao Xavier","doi":"10.17912/micropub.biology.001326","DOIUrl":"https://doi.org/10.17912/micropub.biology.001326","url":null,"abstract":"<p><p>Division of labor, the specialization of subsets of individuals in complementary tasks, increases population efficiency and fitness. We explored swarming motility in <i>Pseudomonas aeruginosa</i> quorum sensing mutants as a model for studying the division of labor. Deletion of the signal synthesis genes <i>lasI</i> or <i>rhlI</i> disrupts swarming, but co-culturing <i>ΔlasI</i> and <i>ΔrhlI</i> restores it in a density-dependent manner. This indicates a successful division of labor where <i>ΔrhlI</i> produces the signal necessary for the <i>ΔlasI</i> mutant, and the <i>ΔlasI</i> reciprocates. We used RNA sequencing to identify additional genes potentially involved in division of labor. Our findings underscore <i>P. aeruginosa</i> swarming as a tractable bacterial model for the division of labor among cells-a hallmark of differentiated multicellularity.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11494437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sterility in the offspring of spr-5; met-2 mutants may be caused by inherited H3K4 methylation and altered germline transcription. spr-5；met-2 突变体后代的不育可能是由遗传的 H3K4 甲基化和种系转录改变引起的。
microPublication biology Pub Date : 2024-10-05 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001365
Jazmin Dozier, Mattie Villhauer, Brandon Carpenter
{"title":"Sterility in the offspring of <i>spr-5; met-2</i> mutants may be caused by inherited H3K4 methylation and altered germline transcription.","authors":"Jazmin Dozier, Mattie Villhauer, Brandon Carpenter","doi":"10.17912/micropub.biology.001365","DOIUrl":"10.17912/micropub.biology.001365","url":null,"abstract":"<p><p>During maternal reprogramming of histone methylation in <i>C. elegans</i> , H3K4me is removed by the histone demethylase, SPR-5 , and H3K9me is subsequently added by the histone methyltransferase, MET-2 . Maternal loss of SPR-5 and MET-2 causes inherited phenotypes, such as sterility, in the progeny. Here, we find that knocking down either the H3K4 methyltransferase SET-2 or the H3K36 methyltransferase MES-4 partially rescues the germline in the progeny of <i>spr-5 ; met-2</i> mutants, suggesting that the inherited sterility may be caused by inherited H3K4 methylation and altered germline transcription.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The yeast gene ECM9 regulates cell wall maintenance and cell division in stress conditions. 酵母基因 ECM9 在应激条件下调节细胞壁的维护和细胞分裂。
microPublication biology Pub Date : 2024-10-01 eCollection Date: 2024-01-01 DOI: 10.17912/micropub.biology.001313
Prevena Ramakrishnan, Jill Keeney
{"title":"The yeast gene ECM9 regulates cell wall maintenance and cell division in stress conditions.","authors":"Prevena Ramakrishnan, Jill Keeney","doi":"10.17912/micropub.biology.001313","DOIUrl":"https://doi.org/10.17912/micropub.biology.001313","url":null,"abstract":"<p><p><i>Saccharomyces cerevisiae</i> , Baker's yeast, is a well-studied model eukaryotic organism. Much of our knowledge about eukaryotic cell function comes from yeast studies, though nearly 10% of yeast genes remain uncharacterized. This study focuses on YKR004C, a verified gene of unknown function named <i>ECM9</i> , predicted to be involved in cell division and cell wall maintenance or composition based on previous studies. We investigated the sensitivity in stress conditions of an <i>ECM9</i> deletion strain, compared to wild-type, to cell wall integrity. These results suggest that <i>ECM9</i> is involved in cell wall maintenance and the regulatory pathway determining cell division readiness under stress.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2024 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11480822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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