microPublication biology最新文献_第4页

Velvety tree ant extract is a chemotaxis repellent for C. elegans. 丝绒树蚁提取物对秀丽隐杆线虫具有趋化性驱避作用。

microPublication biology Pub Date : 2025-05-31 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001531

Malaya Gaerlan, Marco Carrillo, Sofia Ceva, Sowmya Chundi, Binta Diallo, Juliana N Fong, Kelly Huang, Jennifer Jackson, Jasmine Padilla, Leslie Quintana, Katelyn Santa Maria, Sadie M Sarkisian, Paloma R Sequeira, Eva U Tatlock, Penelope R Baker, Luise Bachmann, Soyeon Park, Malia J Perez, Mina E Phipps, Shay Nair Sharma, Yvette Soto-Hernandez, Bryan H Juarez, Cesar Mena, Griselda Morales, Mabel Gonzalez, Katherine Fiocca, Nicole Bradon, Max Madrzyk, Lauren A O'Connell

{"title":"Velvety tree ant extract is a chemotaxis repellent for C. elegans.","authors":"Malaya Gaerlan, Marco Carrillo, Sofia Ceva, Sowmya Chundi, Binta Diallo, Juliana N Fong, Kelly Huang, Jennifer Jackson, Jasmine Padilla, Leslie Quintana, Katelyn Santa Maria, Sadie M Sarkisian, Paloma R Sequeira, Eva U Tatlock, Penelope R Baker, Luise Bachmann, Soyeon Park, Malia J Perez, Mina E Phipps, Shay Nair Sharma, Yvette Soto-Hernandez, Bryan H Juarez, Cesar Mena, Griselda Morales, Mabel Gonzalez, Katherine Fiocca, Nicole Bradon, Max Madrzyk, Lauren A O'Connell","doi":"10.17912/micropub.biology.001531","DOIUrl":"10.17912/micropub.biology.001531","url":null,"abstract":"Ants use a range of compounds for interspecies interactions, but the neurogenetic mechanisms mediating these interactions are unclear. Here, we used chemotaxis assays with the nematode Caenorhabditis elegans to test if ant compounds can be detected by the worm nervous system and which chemosensory neurons are required for detection. We found that C. elegans avoid the extracts of velvety tree ants ( Liometopum occidentale ), and this response requires osm-9 and tax-4 positive neurons. These experiments were conducted by undergraduate students in an upper-division laboratory course, demonstrating how simple behavior assays conducted in a classroom setting can provide practical research experiences and new insights into interspecies interactions.","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Chromosome End Without Terminal Telomere Repeats is Stable for Multiple Cell Divisions. 没有末端端粒重复的染色体末端对多次细胞分裂是稳定的。

microPublication biology Pub Date : 2025-05-30 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001622

Haitao Zhang, Julien Audry, Kurt W Runge

引用次数: 0

Quantifying phage-bacteria dynamics in vitro: rapid emergence of phage-resistant mutants for Klebsiella pneumoniae. 体外噬菌体-细菌动力学定量：肺炎克雷伯菌噬菌体耐药突变体的快速出现。

microPublication biology Pub Date : 2025-05-30 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001666

Marcos Rodríguez, Irene Cantallops, Pilar Domingo-Calap, Josep Sardanyés

{"title":"Quantifying phage-bacteria dynamics in vitro: rapid emergence of phage-resistant mutants for Klebsiella pneumoniae.","authors":"Marcos Rodríguez, Irene Cantallops, Pilar Domingo-Calap, Josep Sardanyés","doi":"10.17912/micropub.biology.001666","DOIUrl":"10.17912/micropub.biology.001666","url":null,"abstract":"In the quantitative description of evolving phage-bacterial systems, a central challenge lies in accurately identifying the key parameters governing the dynamics of both bacterial and phage populations. This is especially relevant in the case of multidrug-resistant pathogenic bacteria such as Klebsiella sp . This pathogen poses serious health problems due to antibiotic overuse, which causes the emergence of antibiotic-resistant strains and great difficulty in eradicating bacterial infections with antibiotics. Research on phage-bacteria thus becomes a very important topic to provide alternative strategies to eradicate multidrug-resistant bacteria, and thus quantitative descriptions of these processes are of paramount importance. Despite increasing research on this topic, key structural parameters of the populations, such as bacterial growth rates, the impact of phages on bacterial dynamics or the probability of emergence of phage-resistant strains, are often scarce. In this study, we investigated a battery of growth experiments for Klebsiella pneumoniae alone and with the presence of bacteriophage vB_Kpn_2-P4. Using mathematical models we estimate key parameters for these experiments, showing the rapid growth and emergence of phage-resistant mutants which outcompete the susceptible bacteria strains. Our results provide quantitative estimates of these processes and may be useful for understanding phage-bacterial dynamical systems and parameterizing future theoretical and computational models.","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12174997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144328009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic expression of endoplasmic reticulum proteins in C. elegans neurons is prone to causing ER stress. 秀丽隐杆线虫神经元内质网蛋白的转基因表达容易引起内质网应激。

microPublication biology Pub Date : 2025-05-29 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001547

Junhyun Park, Shaul Yogev

引用次数: 0

Genome Sequence of the Cluster EF Bacteriophage TinyMiny, Isolated using Microbacterium foliorum. 叶状微杆菌分离的簇EF噬菌体TinyMiny的基因组序列。

microPublication biology Pub Date : 2025-05-29 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001488

Iain Duffy, Juan Jimenez, Luis Gomez, Alexa Keeler, Aisha Ambrose, Moises Guadarrama, Hailey Kerns, Rachael Martin, Jade Moorehead, Arrianne Orgill, Brianna Reiber, Aisha Severe, Leslie Sivley, Genesis Valentin, John Duncan

引用次数: 0

Extracellular stimulation in neocortex lacks specificity. 新皮层的细胞外刺激缺乏特异性。

microPublication biology Pub Date : 2025-05-28 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001539

Linn K Dragsted, Shawniya Alageswaran, Aparna Suvrathan, P Jesper Sjöström

引用次数: 0

Flint Hills Phages: Isolation Parameters and Genomic Characteristics of 23 Phages. Flint Hills噬菌体：23个噬菌体的分离参数和基因组特征。

microPublication biology Pub Date : 2025-05-28 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001461

Martha Smith-Caldas, Christopher Herren, Victoria Thurm, Dasik Clouse, Aidan Ozga, Elizabeth Herrman, Ashley Hine, Kyla Hornback, Nathan Jones, Erika Kline, Lexi Pitts, Rece Buckmaster, Camille Carrier, Marie Dios, MacKenzie Dunigan, Selah Hageman, Maddy Kang, Molly Kang, Madison Sims, Landon Taylor, Bailey Wilson, Taz Zeigler

引用次数: 0

Olfactory cues elicit species-specific locomotive responses in poison frog tadpoles. 嗅觉线索在毒蛙蝌蚪中引起物种特异性的运动反应。

microPublication biology Pub Date : 2025-05-27 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001532

Mina E Phipps, Penelope R Baker, Luise Bachmann, Soyeon Park, Malia J Perez, Shay Nair Sharma, Yvette Soto-Hernandez, Malaya Gaerlan, Marco Carrillo, Sofia Ceva, Sowmya Chundi, Binta Diallo, Juliana N Fong, Kelly Huang, Jennifer Jackson, Jasmine Padilla, Leslie Quintana, Katelyn Santa Maria, Sadie M Sarkisian, Paloma R Sequeira, Eva U Tatlock, Bryan H Juarez, Najva Akbari, Max Madrzyk, Lauren A O'Connell

{"title":"Olfactory cues elicit species-specific locomotive responses in poison frog tadpoles.","authors":"Mina E Phipps, Penelope R Baker, Luise Bachmann, Soyeon Park, Malia J Perez, Shay Nair Sharma, Yvette Soto-Hernandez, Malaya Gaerlan, Marco Carrillo, Sofia Ceva, Sowmya Chundi, Binta Diallo, Juliana N Fong, Kelly Huang, Jennifer Jackson, Jasmine Padilla, Leslie Quintana, Katelyn Santa Maria, Sadie M Sarkisian, Paloma R Sequeira, Eva U Tatlock, Bryan H Juarez, Najva Akbari, Max Madrzyk, Lauren A O'Connell","doi":"10.17912/micropub.biology.001532","DOIUrl":"10.17912/micropub.biology.001532","url":null,"abstract":"Amphibian species rear their larvae in distinct environments that may influence how they respond to different sensory stimuli. Here, we investigated the olfactory-mediated locomotive responses of two poison frog species ( Allobates femoralis and Ranitomeya imitator ) that vary in life history strategies. We found that A. femoralis tadpoles spent more time near an injury cue compared to control, while R. imitator tadpoles increased their movement in response to high concentrations of amino acids. These experiments were done in an undergraduate laboratory course, demonstrating how simple behavior assays conducted in a classroom setting can provide practical research experiences and new insights into animal behavior.","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Bacillus cereus Group from the Fecal Material of Turtles. 龟粪中蜡样芽孢杆菌群的分离。

microPublication biology Pub Date : 2025-05-26 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001587

Jenna L Storz, Durward L Bevis, Richard W McLaughlin

引用次数: 0

Valeric acid attracts C. elegans by activating the AWC neurons through a tax-4 -dependent signaling pathway. 戊酸通过一个依赖于税收-4的信号通路激活AWC神经元，从而吸引秀丽隐杆线虫。

microPublication biology Pub Date : 2025-05-24 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001630

Sreyan Sarkar, Lucero E Rogel-Hernandez, Theresa Logan-Garbisch, Emily Fryer, Victoria Johnson, Miriam B Goodman

{"title":"Valeric acid attracts C. elegans by activating the AWC neurons through a tax-4 -dependent signaling pathway.","authors":"Sreyan Sarkar, Lucero E Rogel-Hernandez, Theresa Logan-Garbisch, Emily Fryer, Victoria Johnson, Miriam B Goodman","doi":"10.17912/micropub.biology.001630","DOIUrl":"10.17912/micropub.biology.001630","url":null,"abstract":"Medicinal plants of the Valeriana genus have been traditionally used around the world to treat several nervous system disorders, yet our understanding of how they do so remains poorly understood. To deepen the understanding of their ability to influence nervous system pathways, we explored the ability of the model organism Caenorhabditis elegans to chemotax to crude extracts of Valeriana officinalis and found that C. elegans are weakly attracted to it. Upon investigating which chemical entities give rise to this behavior, we identified valeric acid (VA) as a primary candidate. Through chemotaxis assays, we show that wild-type C. elegans are strongly attracted to VA in a dose-dependent manner. Chemotaxis assays with mutant strains of C. elegans deficient in chemosensation indicate that the tax-4 -dependent nervous pathways are most heavily responsible for detecting VA. However, osm-9 -dependent pathways may also play a small role in regulating the worm's response to VA. Additionally, animals lacking AWC neurons are indifferent to this compound, and therefore, future research should focus on what molecular entities grant the AWC neurons the ability to detect VA.","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0