microPublication biologyPub Date : 2026-09-19eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002349
Sarah Perry, Frankie Davis, Vishva Patel, Rudy Alvarado, Mckinley Black, Jefferson Bretanys Desca, Mason Briggs, Jackson Buck, Jontasia Campbell, Emma Carter, Dakota Clark, Aleandrjo Collins, Caleb Curtis, Sydney Denker, Machi Gillard, Lexie Goldammer, Tristen Gorman, Cameron Grayson, Daisy Heller, Kindall Holmes Cerveny, Josearmando Izaguirre, Colton James, Kelbe Jeffries, Seth Johnson, Elisha Keeler, Phoenix Kincade, Eric Lambert, Daniela Malagon, Emily Marks, Joseph Mcgeshick, Sarah McKinney, Zoey Melendrez, Gabriella Mignano, Jackson Miller, Hannah Miller, Jesse Perales, Cheena Pun, Trent Rollet, Shekinah Ross, David Rourke, Christian Ryan, Corey Slinker, Zoe Spann-Mcdonald, Jasper Spurk, Madelyn Steele, Emme Sylvester, Sarah Turpen, Tahmar Upshaw
{"title":"Utilizing a <i>Drosophila</i> Larval Model of Synucleopathy to Explore Mushroom Extract Treatments for Parkinson's Disease.","authors":"Sarah Perry, Frankie Davis, Vishva Patel, Rudy Alvarado, Mckinley Black, Jefferson Bretanys Desca, Mason Briggs, Jackson Buck, Jontasia Campbell, Emma Carter, Dakota Clark, Aleandrjo Collins, Caleb Curtis, Sydney Denker, Machi Gillard, Lexie Goldammer, Tristen Gorman, Cameron Grayson, Daisy Heller, Kindall Holmes Cerveny, Josearmando Izaguirre, Colton James, Kelbe Jeffries, Seth Johnson, Elisha Keeler, Phoenix Kincade, Eric Lambert, Daniela Malagon, Emily Marks, Joseph Mcgeshick, Sarah McKinney, Zoey Melendrez, Gabriella Mignano, Jackson Miller, Hannah Miller, Jesse Perales, Cheena Pun, Trent Rollet, Shekinah Ross, David Rourke, Christian Ryan, Corey Slinker, Zoe Spann-Mcdonald, Jasper Spurk, Madelyn Steele, Emme Sylvester, Sarah Turpen, Tahmar Upshaw","doi":"10.17912/micropub.biology.002349","DOIUrl":"https://doi.org/10.17912/micropub.biology.002349","url":null,"abstract":"<p><p>Parkinson's disease (PD) is characterized by dopaminergic neuron loss and progressive motor dysfunction. Here, we incorporated a <i>Drosophila</i> larval model of α-synucleinopathy into a course-based undergraduate research experience (CURE) to investigate the effects of mushroom extract supplementation on locomotor dysfunction. Larvae expressing human A53T α-synuclein in dopaminergic neurons exhibited impaired Ring Maze performance. Dietary supplementation with Lion's mane ( <i>Hericium erinaceus</i> ) improved overall Ring Maze performance in A53T larvae; however, Lion's mane also enhanced performance in TH-Gal4 controls, suggesting a broader locomotor-enhancing effect rather than an A53T-specific rescue. In contrast, vitamin C improved performance in A53T larvae without a detectable beneficial effect in controls. Turkey tail ( <i>Trametes versicolor</i> ) and Chaga ( <i>Inonotus obliquus</i> ) did not consistently improve A53T performance. These findings identify Lion's mane as an interesting candidate for further study while demonstrating the utility of the Ring Maze for undergraduate investigation of neurodegenerative disease models and treatment effects.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13634202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-17eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002002
Kit Williams, Emily Savage
{"title":"Genome Sequence of Novel Cluster GF Bacteriophage Wrackline, Isolated on <i>Microbacterium sp.</i> Casco Bay.","authors":"Kit Williams, Emily Savage","doi":"10.17912/micropub.biology.002002","DOIUrl":"https://doi.org/10.17912/micropub.biology.002002","url":null,"abstract":"<p><p>Wrackline is a novel bacteriophage isolated from sand collected along the high tide line and beneath organic material on Willard Beach, ME. It was isolated using <i>Microbacterium sp.</i> strain Casco Bay, a marine bacterium, and is also capable of infecting <i>Microbacterium foliorum,</i> a bacterium originally isolated from grass. Wrackline's genome is 42,148 bp and encodes 80 putative protein coding genes, 26 of which have no known homologs. Based on gene content similarity, Wrackline is assigned to actinobacteriophage cluster GF.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genome Sequence of Bacteriophage Myram Isola---ted With Host <i>Microbacterium foliorum</i>.","authors":"Solomon DeMello, Peyton Guay, Brooke Souder, Zach Stillman, Bekhruz Avganov, Jae Howell, Kit Williams, Emily Savage","doi":"10.17912/micropub.biology.002004","DOIUrl":"https://doi.org/10.17912/micropub.biology.002004","url":null,"abstract":"<p><p>Bacteriophage Myram was isolated from sandy soil in South Portland, ME on <i>Microbacterium foliorum</i> NRRL B-24224. It has a siphovirus morphology and a 17,362-bp genome encoding 25 putative genes. Based on shared gene content, Myram is assigned to actinobacteriophage cluster EE.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-17eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002361
Yuzuha Komachiya, Akane Matsumura, Natsu Sato, Riho Kato, Hayao Ohno
{"title":"An ultra-rapid mounting method for <i>C. elegans</i> embryos that enables long-term, multi-sample live imaging.","authors":"Yuzuha Komachiya, Akane Matsumura, Natsu Sato, Riho Kato, Hayao Ohno","doi":"10.17912/micropub.biology.002361","DOIUrl":"https://doi.org/10.17912/micropub.biology.002361","url":null,"abstract":"<p><p>Live imaging of <i>Caenorhabditis elegans</i> embryos often requires labor-intensive dissection or mounting procedures. Here we present a versatile method in which nematode growth medium (NGM) agar chunks containing laid embryos are excised and inverted directly onto coverslips or chamber slides. Preparation requires less than one minute, enabling immediate fluorescence and transmitted-light microscopy on both inverted and upright systems. Moisture loss is minimized by using a protective cap or chamber lid, permitting uninterrupted 24-hour time-lapse imaging. Combining multi-well slides with a motorized stage allows simultaneous multi-sample tracking. This rapid approach streamlines live-imaging genetic screens and developmental analyses.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-16eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002381
Takako Kato-Minoura, Ichiro Sasaki, Ritsu Kamiya
{"title":"Novel revertants of <i>Chlamydomonas vfl2</i> suggest a role for a flexible central α-helical region in centrin function in flagellar basal bodies.","authors":"Takako Kato-Minoura, Ichiro Sasaki, Ritsu Kamiya","doi":"10.17912/micropub.biology.002381","DOIUrl":"https://doi.org/10.17912/micropub.biology.002381","url":null,"abstract":"<p><p>A <i>Chlamydomonas</i> mutant, <i>vfl2</i> , displays aberrant flagellar numbers due to an E101K mutation in the centrin gene. We isolated 21 revertants-12 intragenic and 9 extragenic-that suppress the mutant's abnormal phenotype. AlphaFold2 modeling indicates that the wild-type, mutant, and revertant proteins are structurally similar. However, the <i>vfl2</i> mutant centrin forms three salt bridges in the central region of the core α-helix, whereas the wild type and all intragenic revertants have only two salt bridges or disrupted helix continuity. These results indicate that flexibility of the central helix, maintained by limited electrostatic interactions, is important for proper centrin function.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13628304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-16eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002355
Julian Flanagan, Amanda K Hund, Joseph Chihade
{"title":"Gene models of four <i>Schistocephalus solidus</i> aminoacyl-tRNA synthetases.","authors":"Julian Flanagan, Amanda K Hund, Joseph Chihade","doi":"10.17912/micropub.biology.002355","DOIUrl":"https://doi.org/10.17912/micropub.biology.002355","url":null,"abstract":"<p><p>Aminoacyl-tRNA synthetases (ARSs) are the enzymes responsible for pairing tRNAs with amino acids during protein synthesis. Despite their conserved function, important features of these enzymes vary between species. Because previous research on animal ARSs has predominantly focused on human enzymes, relatively little is known about ARSs from non-vertebrate parasites. To examine helminth-specific ARS features, we used available genomic and RNA-seq data to produce corrected gene models of four ARSs from <i>Schistocephalus solidus</i> , a cestode parasite. Predicted gene models were verified and extended by PCR amplification and sequencing of <i>S. solidus</i> cDNA, revealing single nucleotide variations we attribute to geographical variation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13628303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-16eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002357
Benjamin Galligos, Giovanna Caime, Alexis Michalski, Jamie Dyer
{"title":"<i>Drosophila</i> <i>l(2)23CDe <sup>A8-1</sup></i> and <i>l(2)23CDf <sup>A18-2</sup></i> are missense alleles of the <i>SerRS</i> gene.","authors":"Benjamin Galligos, Giovanna Caime, Alexis Michalski, Jamie Dyer","doi":"10.17912/micropub.biology.002357","DOIUrl":"https://doi.org/10.17912/micropub.biology.002357","url":null,"abstract":"<p><p>Viability requires that certain genes are expressed at the correct levels and at specific times during development. Many genes have already been determined as essential to development, but many more may exist. Numerous lethal mutations have been isolated in <i>Drosophila melanogaster</i> that have yet to be identified at the gene level. Here we provide deficiency mapping, complementation testing, and DNA sequencing that suggests the unknown lethal mutations <i>l(2)23CDe <sup>A8-1</sup></i> and <i>l(2)23CDf <sup>A18-2</sup></i> are both missense mutations in the <i>SerRS</i> gene, which encodes an aminoacyl tRNA synthetase that is required for protein translation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13628305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-15eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002348
Hope Freshwater, Pablo Chialvo, Clare Scott Chialvo
{"title":"Gene model for the ortholog of <i>yellow-f2</i> in <i>Drosophila dunni</i>.","authors":"Hope Freshwater, Pablo Chialvo, Clare Scott Chialvo","doi":"10.17912/micropub.biology.002348","DOIUrl":"https://doi.org/10.17912/micropub.biology.002348","url":null,"abstract":"<p><p>We developed a gene model for the <i>yellow-f2</i> ortholog in the ASM1815212v1 Genome Assembly (GenBank Accession: GCA_018152125.1 ) of <i>Drosophila dunni</i> . This ortholog was characterized as part of a developing dataset for a comparative study of detoxification gene family evolution in the <i>immigrans</i> - <i>tripunctata</i> radiation of the genus <i>Drosophila</i> using an adapted Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-15eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.001875
Ellie Clavette, Andrea Chuva, Nicholas Gruener, Juniper Zinck, Sharon Gusky
{"title":"Genome Sequence of Mycobacteriophage Lilbit.","authors":"Ellie Clavette, Andrea Chuva, Nicholas Gruener, Juniper Zinck, Sharon Gusky","doi":"10.17912/micropub.biology.001875","DOIUrl":"https://doi.org/10.17912/micropub.biology.001875","url":null,"abstract":"<p><p>Mycobacteriophage Lilbit was isolated using <i>Mycobacterium smegmatis</i> mc²155. It has a genome consisting of 65,106 base pairs with 110 putative genes and GC content of 63.4%. Based on gene content similarity, it is assigned to actinobacteriophage cluster S. Functions were predicted for 40 genes. There were no tRNAs identified in the genome.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
microPublication biologyPub Date : 2026-09-15eCollection Date: 2026-01-01DOI: 10.17912/micropub.biology.002375
Hailey X Egido-Betancourt, Cheyenne E Brown, Farr Niere, Jyoti Iyer
{"title":"A commercially available DJ-1 antibody does not detect <i>C. elegans</i> DJR-1.1 or DJR-1.2 proteins in whole worm lysates.","authors":"Hailey X Egido-Betancourt, Cheyenne E Brown, Farr Niere, Jyoti Iyer","doi":"10.17912/micropub.biology.002375","DOIUrl":"https://doi.org/10.17912/micropub.biology.002375","url":null,"abstract":"<p><p>Custom antibody synthesis is both expensive and time-consuming. Therefore, the ability to repurpose existing commercially available antibodies to detect their corresponding <i>C. elegans</i> orthologs can be very beneficial. Here, we attempted to use the commercially available DJ-1 antibody NB300-270 to detect <i>C. elegans</i> DJR-1.1 and DJR-1.2 proteins in whole worm lysates. Our results indicate that the NB300-270 DJ-1 antibody does not detect <i>C. elegans</i> DJR-1.1 or DJR-1.2 proteins in whole worm lysates <i>.</i></p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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