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Possible Horizontal Gene Transfer of Novel Transposable Elements in Anisakis simplex between Hosts and Parasites. 单一异尖线虫新转座因子在宿主和寄生虫间可能的水平基因转移。
microPublication biology Pub Date : 2025-09-02 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001578
Naoki Hoshino, Hiroki Kuroda
{"title":"Possible Horizontal Gene Transfer of Novel Transposable Elements in <i>Anisakis simplex</i> between Hosts and Parasites.","authors":"Naoki Hoshino, Hiroki Kuroda","doi":"10.17912/micropub.biology.001578","DOIUrl":"10.17912/micropub.biology.001578","url":null,"abstract":"<p><p><i>Tc1</i> / <i>mariner</i> transposons found in salmoniform fish have been identified in both closely and distantly related fish species, suggesting that horizontal gene transfer may have occurred. However, the vectors of this process remain unknown. We identified two homologous sequences in the parasitic nematode <i>Anisakis simplex</i> , naming them <i>Tas1</i> ( T ransposable element of <i>A nisakis s implex</i> number 1 ) and <i>Tas2</i> . These elements encode <i>Tc1</i> / <i>mariner</i> transposases structurally similar to the active <i>Sleeping Beauty</i> transposase. Furthermore, <i>Tas1</i> / <i>2</i> were also identified in organisms that serve as hosts for <i>Anisakis</i> . These findings suggest that <i>Tas1</i> / <i>2</i> may have undergone horizontal gene transfer within host-parasite interactions.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FHL2 fused with biotin ligase shuttles between focal adhesions and the nucleus in a myosin II-dependent manner. 与生物素连接酶融合的FHL2以肌球蛋白ii依赖的方式在局灶黏附物和细胞核之间穿梭。
microPublication biology Pub Date : 2025-08-28 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001669
Yukari Fujimoto, Masaya Fujimoto, Daijiro Konno, Naotaka Nakazawa
{"title":"FHL2 fused with biotin ligase shuttles between focal adhesions and the nucleus in a myosin II-dependent manner.","authors":"Yukari Fujimoto, Masaya Fujimoto, Daijiro Konno, Naotaka Nakazawa","doi":"10.17912/micropub.biology.001669","DOIUrl":"10.17912/micropub.biology.001669","url":null,"abstract":"<p><p>Four-and-a-half LIM domains 2 (FHL2) is a molecule that plays a key role in cell proliferation in response to mechanical signals. It shuttles between focal adhesions (FAs) or stress fibers (SFs) and the nucleus in a force-dependent manner. FHL2 interacts with other cytoskeletal molecules at FAs and SFs, but FHL2 interacts with transcriptional factors in the nucleus. This leads to modulation of gene expression for cell proliferation. However, the overall picture of interacting proteins with FHL2 at different regions is poorly understood. Here, we report a stable cell line that expresses FHL2-GFP-BirA, a fusion protein of FHL2 with biotin ligase. FHL2-GFP-BirA localizes at the FAs but accumulates in the nucleus after myosin II inhibition, exhibiting behavior similar to endogenous FHL2. These results suggest that the BioID technique can be used to identify proteins interacting with FHL2 under different mechanical conditions.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The embryonic lethal mutation zyg-10(b261) is an allele of the atx-2 gene and disrupts multiple aspects of early embryogenesis. 胚胎致死性突变zyg-10（b261）是atx-2基因的一个等位基因，破坏了早期胚胎发生的多个方面。
microPublication biology Pub Date : 2025-08-28 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001714
Zachary G Bell, Harold E Smith, Kevin F O'Connell
{"title":"The embryonic lethal mutation <i>zyg-10(b261)</i> is an allele of the <i>atx-2</i> gene and disrupts multiple aspects of early embryogenesis.","authors":"Zachary G Bell, Harold E Smith, Kevin F O'Connell","doi":"10.17912/micropub.biology.001714","DOIUrl":"10.17912/micropub.biology.001714","url":null,"abstract":"<p><p>The <i>zyg-10 ( b261 )</i> mutation was identified in one of the earliest screens for temperature-sensitive embryonic lethal mutations in <i>C. elegans</i> , but the cytological defects underlying the embryonic lethal phenotype, as well as the molecular identity of <i>zyg-10</i> had not been previously established. Here we show that <i>zyg-10 ( b261 )</i> is an allele of the <i>atx-2</i> (ataxin-related) gene and that embryos produced by <i>atx-2 ( b261 )</i> mothers exhibit a variety of defects including eggshell defects, cytokinesis failure, spindle mispositioning, and chromosome missegregation. We also show that the localization of separase, a regulator of egg-shell formation and mitosis, is defective in <i>atx-2 ( b261 )</i> embryos.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parthenolide acts as a cocaine antagonist in the human dopamine transporter. Parthenolide在人类多巴胺转运体中起可卡因拮抗剂的作用。
microPublication biology Pub Date : 2025-08-27 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001720
Oné R Pagán
{"title":"Parthenolide acts as a cocaine antagonist in the human dopamine transporter.","authors":"Oné R Pagán","doi":"10.17912/micropub.biology.001720","DOIUrl":"10.17912/micropub.biology.001720","url":null,"abstract":"<p><p>In this work, I describe the effects of parthenolide, a naturally occurring sesquiterpene lactone, on radiolabeled cocaine binding and on radiolabeled dopamine uptake in the human dopamine transporter expressed on HEK-293 cells (HEK/DAT cells). Parthenolide displaces radiolabeled cocaine in a concentration-dependent manner and does not display any significant inhibition of radiolabeled dopamine uptake. Further, parthenolide seems to alleviate the inhibition of dopamine uptake by 5 μM unlabeled cocaine at sub-toxic parthenolide concentrations.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sexually dimorphic control of crossover distribution by the conserved ATPase PCH-2 in C. elegans. 保守atp酶PCH-2对线虫交叉分布的两性二态控制。
microPublication biology Pub Date : 2025-08-25 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001802
Alberto Herrera, Bianca Pacheco, Bhumil Patel, Kealani Holland, Needhi Bhalla
{"title":"Sexually dimorphic control of crossover distribution by the conserved ATPase PCH-2 in <i>C. elegans</i>.","authors":"Alberto Herrera, Bianca Pacheco, Bhumil Patel, Kealani Holland, Needhi Bhalla","doi":"10.17912/micropub.biology.001802","DOIUrl":"10.17912/micropub.biology.001802","url":null,"abstract":"<p><p>Meiotic crossover recombination is essential for accurate chromosome segregation and the creation of new allele combinations that drive natural selection during evolution. Thus, the number and distribution of crossovers is exquisitely controlled. We have shown that the pachytene checkpoint component and conserved AAA-ATPase PCH-2 controls crossover number and distribution during oogenesis in <i>C. elegans</i> . To test if PCH-2 has similar effects during spermatogenesis, we monitored recombination across a single chromosome in control and <i>pch-2</i> mutant males. Our results demonstrate that PCH-2 's effect on crossover distribution during spermatogenesis is different than we observed in oogenesis, exhibiting sexual dimorphism.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptome changes caused by loss of WAGO-4 is remembered in wild type offspring. WAGO-4缺失引起的转录组变化在野生型后代中被记住。
microPublication biology Pub Date : 2025-08-25 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001769
Ann-Sophie Seistrup, Rene Ketting, Jan Schreier, Ida Isolehto
{"title":"Transcriptome changes caused by loss of WAGO-4 is remembered in wild type offspring.","authors":"Ann-Sophie Seistrup, Rene Ketting, Jan Schreier, Ida Isolehto","doi":"10.17912/micropub.biology.001769","DOIUrl":"10.17912/micropub.biology.001769","url":null,"abstract":"<p><p>The worm-specific Argonaute WAGO-4 has been shown to be involved in epigenetic memory in <i>C. elegans</i> , however, its mode of action remains unclear. Here, we use a <i>wago-4</i> deletion mutant to show that general changes to 22G-RNAs, the small RNA cofactor binding WAGO-4 , do not correlate with changes to mRNA levels. We also show that the function of WAGO-4 differs in L4 larvae and in adult worms, and, importantly, we show that mRNA misregulation caused by loss of WAGO-4 persists in wild-type offspring of deletion mutants for at least five generations.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The poly-proline motifs found in the cytoplasmic tail of the brush border cadherin CDHR5 play a role in its ability to elongate microvilli. 在刷状边界钙粘蛋白CDHR5的细胞质尾部发现的聚脯氨酸基序在其延长微绒毛的能力中发挥作用。
microPublication biology Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001663
Samaneh Matoo, Prashun Acharya, Sadika T J Tonu, Jasvinder Bharaj, Ashwini Mudaliyar, Basmala Touny, Regan VanderPol, Morgan Timms, Nicole Dyko, Domtie Asante, Hawa Abdulle, Scott W Crawley
{"title":"The poly-proline motifs found in the cytoplasmic tail of the brush border cadherin CDHR5 play a role in its ability to elongate microvilli.","authors":"Samaneh Matoo, Prashun Acharya, Sadika T J Tonu, Jasvinder Bharaj, Ashwini Mudaliyar, Basmala Touny, Regan VanderPol, Morgan Timms, Nicole Dyko, Domtie Asante, Hawa Abdulle, Scott W Crawley","doi":"10.17912/micropub.biology.001663","DOIUrl":"10.17912/micropub.biology.001663","url":null,"abstract":"<p><p>Cadherin-related family member 5 (CDHR5) is a protocadherin found enriched at the tips of brush border microvilli of the gut and kidney, where it plays an important role in the development of these specialized microvilli. CDHR5 is a type-1 transmembrane protein with a short cytoplasmic tail that contains a number of poly-proline motifs of unknown function. We performed an analysis of the poly-proline stretches in the CDHR5 cytoplasmic tail and show that mutation of these motifs does not largely influence the targeting of CDHR5 to microvilli, but does significantly impact the ability of the cadherin to promote microvillar elongation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Use of blue fluorescent protein Electra2 for live-cell imaging in Dictyostelium discoideum. 蓝色荧光蛋白electr2在盘状盘基ostelium distyostelum活细胞成像中的应用。
microPublication biology Pub Date : 2025-08-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001774
Hidenori Hashimura, Hibiki Nakagawa, Satoshi Sawai
{"title":"Use of blue fluorescent protein Electra2 for live-cell imaging in <i>Dictyostelium discoideum</i>.","authors":"Hidenori Hashimura, Hibiki Nakagawa, Satoshi Sawai","doi":"10.17912/micropub.biology.001774","DOIUrl":"10.17912/micropub.biology.001774","url":null,"abstract":"<p><p>Because of its good spectral separation from green (GFP) and red (RFP) fluorescent proteins, blue fluorescent protein (BFP) is essential for multicolor live cell imaging. However, the commonly used bright mTagBFP2 strongly perturbs the cellular localization of Lifeact, an F-actin marker. As an alternative, we tested the expression of Electra2 in <i>Dictyostelium</i> . Both standalone and as a fusion tag to Lifeact, HistoneH1, or the Akt/PKB PH domain, Electra2 showed brightness comparable to that of mTagBFP2, with intracellular localization patterns consistent with those of GFP and RFP. Electra2 is a promising BFP of choice for studying actin and other targets.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
C. albicans pseudouridine glycosidase is important for growth in hygromycin stress and for filamentation. 白色念珠菌假尿苷糖苷酶对潮霉素胁迫下的生长和丝化很重要。
microPublication biology Pub Date : 2025-08-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001713
Rheanna E Walther, Emeline Singh, Douglas A Bernstein
{"title":"<i>C. albicans</i> pseudouridine glycosidase is important for growth in hygromycin stress and for filamentation.","authors":"Rheanna E Walther, Emeline Singh, Douglas A Bernstein","doi":"10.17912/micropub.biology.001713","DOIUrl":"10.17912/micropub.biology.001713","url":null,"abstract":"<p><p>When an RNA is no longer needed or has become damaged, it is degraded to its single base components. Pseudouridine is found in all domains of life and is found in a variety of types of RNA. Pseudouridine has ribose and uracil moieties attached via a C-C bond. Cleavage of this bond is performed by pseudouridine glycosidases. We find deletion of the pseudouridine glycosidase from the human fungal pathogen <i>C. albicans</i> leads to sensitivity to hygromycin and changes to filamentation. In addition, deletion of pseudouridine glycosidase leads to the upregulation of several permeases. Our data suggest pseudouridine glycosidases are important for <i>C. albicans</i> fitness.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol: An absolute egg-to-adult viability assay in Drosophila melanogaster. 实验方案：黑腹果蝇卵到成虫的绝对活力测定。
microPublication biology Pub Date : 2025-08-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001656
Cassidy Schneider, Spencer Koury, Laurie S Stevison
{"title":"Protocol: An absolute egg-to-adult viability assay in <i>Drosophila melanogaster</i>.","authors":"Cassidy Schneider, Spencer Koury, Laurie S Stevison","doi":"10.17912/micropub.biology.001656","DOIUrl":"10.17912/micropub.biology.001656","url":null,"abstract":"<p><p>Measures of organismal fitness must take into account reproductive output and survivorship across life-history stages. In <i>Drosophila melanogaster</i> , a laboratory model system, these traits are often quantified with egg-laying assays and egg-to-adult viability. While several protocols for automated egg counting exist, these methods typically preclude directly analyzing phenotypic distributions in resulting adults. Here, we digitally score eggs from photographs taken under standard laboratory culturing conditions (cornmeal media in polystyrene vials) using a method compatible with scoring surviving adults. This absolute measurement of egg-to-adult viability, that also allows investigation of survivorship biases, was applied to three commonly used laboratory strains.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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