microPublication biology最新文献

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Soil microbiome analysis of cultivated tomato ( Solanum lycopersicum ) plants. 栽培番茄植株土壤微生物组分析。
microPublication biology Pub Date : 2025-05-23 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001225
Nicolas L Cerioni, Harrison L Uhl, Mara A Welty, Jacob J Adler
{"title":"Soil microbiome analysis of cultivated tomato ( <i>Solanum lycopersicum</i> ) plants.","authors":"Nicolas L Cerioni, Harrison L Uhl, Mara A Welty, Jacob J Adler","doi":"10.17912/micropub.biology.001225","DOIUrl":"10.17912/micropub.biology.001225","url":null,"abstract":"<p><p>Microbial biodiversity is critical to tomato plant health. The symbiotic relationship between tomato plants and their soil microbiome influences the plants' ability to absorb nutrients and adapt to environmental stresses. This study compared the soil microbiome between tomato plants appearing healthy versus those appearing unhealthy. There were no significant differences in overall bacterial biodiversity between the conditions. However, a specific beneficial genus ( <i>Sphingomonas</i> ) and its phylum Proteobacteria (Pseudomonadota) were found at significantly higher amounts in healthy plants' soil compared to unhealthy plants' soil. Our findings show the need for further examination of the benefits of <i>Sphingomonas</i> for tomato plants.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electron microscopy ascertains presence of centrioles in rectal epithelial cells of C. elegans L1 larvae. 电镜观察发现秀丽隐杆线虫L1幼虫直肠上皮细胞中存在中心粒。
microPublication biology Pub Date : 2025-05-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001624
Marie Croisier, Coralie Busso, Nils Kalbfuss, Alexander Woglar, Graham Knott, Pierre Gönczy
{"title":"Electron microscopy ascertains presence of centrioles in rectal epithelial cells of <i>C. elegans</i> L1 larvae.","authors":"Marie Croisier, Coralie Busso, Nils Kalbfuss, Alexander Woglar, Graham Knott, Pierre Gönczy","doi":"10.17912/micropub.biology.001624","DOIUrl":"10.17912/micropub.biology.001624","url":null,"abstract":"<p><p>Centrioles are microtubule-based organelles important for cellular organization and function. <i>C. elegans</i> embryos undergo extensive programmed centriole elimination, with merely 7 post-mitotic cells retaining a focus of the centriolar proteins SAS-4 and SAS-7 in L1 larvae. Here, we addressed whether such foci correspond to <i>bona fide</i> centrioles by conducting serial-section electron microscopy. Our analysis ascertains that centriolar microtubule configurations are indeed present in the B, F, U and Y rectal epithelial cells. Therefore, centrioles are truly spared from elimination in these cells, calling for investigating the importance of such retention compared to the elimination program occurring in most post-mitotic cells.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12138532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Showing the Bonds-A Subtle but Important Difference in Figure Design that May Alleviate Student Confusion about ATP Hydrolysis. 显示键-图形设计中微妙但重要的差异,可以减轻学生对ATP水解的困惑。
microPublication biology Pub Date : 2025-05-22 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001540
Crystal Uminski, Shreya Sujith, Aneesh Nallani, Bryan Armpriest, L Kate Wright, Dina L Newman, Mingyu Yang
{"title":"Showing the Bonds-A Subtle but Important Difference in Figure Design that May Alleviate Student Confusion about ATP Hydrolysis.","authors":"Crystal Uminski, Shreya Sujith, Aneesh Nallani, Bryan Armpriest, L Kate Wright, Dina L Newman, Mingyu Yang","doi":"10.17912/micropub.biology.001540","DOIUrl":"10.17912/micropub.biology.001540","url":null,"abstract":"<p><p>A misconception among biology students is that breaking bonds in adenosine triphosphate (ATP) releases energy. This misconception may be related to imprecise representations of chemical bonding in common diagrams of ATP hydrolysis. We interviewed 33 undergraduate students and randomly assigned them to interpret a figure of ATP hydrolysis that either emphasized bond breaking in the reactants or the formation of new bonds in the products. Students who saw the figure emphasizing bond breaking were more likely to incorrectly classify ATP hydrolysis as endergonic, while students who saw the figure explicitly illustrating bond formation were more likely to use chemically-sound reasoning to describe the reaction.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144251185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome Sequences of Microbacterium foliorum Phages BouleyBill and Carostasia, isolated in France. 法国叶状微细菌噬菌体BouleyBill和Carostasia的基因组序列。
microPublication biology Pub Date : 2025-05-21 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001492
Christophe Regeard, Florence Constantinesco-Becker, Anne Lopes, Ana A Arteni, Malika Ouldali, Laura Pieri, Monique Auberdiac, Daniel Delaruelle, Kevin Tambosco, Hakima Abes, Clément Almeida-Monge, Félix Benard, Lucie Boucard, Elsa Chaouat, Juliette Charazac, Caroline Comte, Marie Coutard, Téo Denis, Clarisse Deschamps-Martin, Erwin Filloux, Anastasia Gaultier, Madeleine Gautheret, Hafsa Harrat, Océane Hill, Mattéo Jalmain, Cécile Jolivet, Diane Le Tyrant, Sarah Lopez, Cléa Medin, Camille Outtier, Mélissa Roze, Maria Rubio-Espinal, May-Blue Zeni, Ombeline Rossier
{"title":"Genome Sequences of <i>Microbacterium foliorum</i> Phages BouleyBill and Carostasia, isolated in France.","authors":"Christophe Regeard, Florence Constantinesco-Becker, Anne Lopes, Ana A Arteni, Malika Ouldali, Laura Pieri, Monique Auberdiac, Daniel Delaruelle, Kevin Tambosco, Hakima Abes, Clément Almeida-Monge, Félix Benard, Lucie Boucard, Elsa Chaouat, Juliette Charazac, Caroline Comte, Marie Coutard, Téo Denis, Clarisse Deschamps-Martin, Erwin Filloux, Anastasia Gaultier, Madeleine Gautheret, Hafsa Harrat, Océane Hill, Mattéo Jalmain, Cécile Jolivet, Diane Le Tyrant, Sarah Lopez, Cléa Medin, Camille Outtier, Mélissa Roze, Maria Rubio-Espinal, May-Blue Zeni, Ombeline Rossier","doi":"10.17912/micropub.biology.001492","DOIUrl":"10.17912/micropub.biology.001492","url":null,"abstract":"<p><p>Bacteriophages BouleyBill and Carostasia, exhibiting siphovirus morphology, were isolated in France. Both infected <i>Microbacterium foliorum</i> strain NRRL B-24224. Their 39,215-bp and 40,393-bp genomes were assigned to subclusters EA4 and EA10, respectively, widening for the first time the known geographical distribution of these subclusters to Europe.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12138531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Qualitative chemical analysis of cinnamon flavored e-cigarette vapor to identify compounds of interest that may affect cardiovascular function. 肉桂味电子烟蒸气的定性化学分析,以确定可能影响心血管功能的化合物。
microPublication biology Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001605
Jennifer M Piechowski, Brian Bagatto
{"title":"Qualitative chemical analysis of cinnamon flavored e-cigarette vapor to identify compounds of interest that may affect cardiovascular function.","authors":"Jennifer M Piechowski, Brian Bagatto","doi":"10.17912/micropub.biology.001605","DOIUrl":"10.17912/micropub.biology.001605","url":null,"abstract":"<p><p>Previous work on the impact of cinnamon flavored electronic cigarette vapor on heart function during early development in zebrafish indicate chemical compounds in the vapor, aside from nicotine, can significantly affect heart function. To determine which compounds were present in the vapor used in our prior study, non-targeted, qualitative gas chromatography/mass spectrometry was performed. Nicotine was found to be present in the nicotine-containing vapor, and it was confirmed to be absent in the nicotine-free vapor. Other chemical compounds that may have affected cardiovascular function in zebrafish were also identified in the analyzed vapors including cinnamaldehyde and eugenol among others.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Curli Accessory Protein CsgF of Salmonella Typhimurium Influences the in vitro Aggregation of Human Islet Amyloid Polypeptide. 鼠伤寒沙门菌Curli附属蛋白CsgF对胰岛淀粉样多肽体外聚集的影响。
microPublication biology Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001565
Osmar Meza-Barajas, Clayton Connelly, Alejandra Lopez, Isamar Aranda, Ashwag Binmahfooz, Allison Newell, Sajith Jayasinghe
{"title":"The Curli Accessory Protein CsgF of <i>Salmonella Typhimurium</i> Influences the in vitro Aggregation of Human Islet Amyloid Polypeptide.","authors":"Osmar Meza-Barajas, Clayton Connelly, Alejandra Lopez, Isamar Aranda, Ashwag Binmahfooz, Allison Newell, Sajith Jayasinghe","doi":"10.17912/micropub.biology.001565","DOIUrl":"10.17912/micropub.biology.001565","url":null,"abstract":"<p><p>The Curli secretion gene product F (CsgF) is a critical component of the assembly of Curli, proteinaceous filaments, found on the outer surface of gram-negative bacteria such as <i>E. Coli</i> and <i>Salmonella</i> . Herein we describe the ability of CsgF to influence the in-vitro aggregation of human islet amyloid polypeptide (hIAPP), an amyloidogenic polypeptide that is unrelated to Curli. In the presence of CsgF no increase in Thioflavin T fluorescence was observed for freshly solubilized hIAPP monitored as a function of time, suggesting that CsgF prevents the aggregation of hIAPP during the period of observation. A variant of CsgF lacking the first 65 residues in the N-terminus of CsgF retained the ability to inhibit the aggregation of hIAPP suggesting that the ability of CsgF to inhibit the aggregation of hIAPP is mediated by the C-terminal half of the protein.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of embryo size on apoptosis in C. elegans. 胚胎大小对秀丽隐杆线虫凋亡的影响。
microPublication biology Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001608
Madiha Javeed Ghani, Jens Van Eeckhoven, Barbara Conradt
{"title":"Impact of embryo size on apoptosis in <i>C. elegans</i>.","authors":"Madiha Javeed Ghani, Jens Van Eeckhoven, Barbara Conradt","doi":"10.17912/micropub.biology.001608","DOIUrl":"10.17912/micropub.biology.001608","url":null,"abstract":"<p><p>During <i>C. elegans</i> development 131 somatic cells reproducibly undergo programmed cell death. Many of these 131 cells 'programmed to die' are the smaller daughter of a neuroblast that divides asymmetrically and die through apoptosis. To determine whether cell size impacts the ability of cells programmed to die to undergo apoptosis, we increased or decreased embryo size by RNA interference-mediated knock-down of the genes <i>C27D9.1</i> or <i>ima-3</i> , respectively. We found that in apoptosis-compromised genetic backgrounds, <i>C27D9.1</i> ( <i>RNAi</i> ) enhances and <i>ima-3</i> ( <i>RNAi</i> ) partially suppresses inappropriate survival of cells programmed to die. This supports the notion that in <i>C. elegans</i> embryos, an increase in cell size compromises and a decrease in cell size promotes the ability of cells programmed to die to undergo apoptosis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comparative analysis of distinct assays for the investigation of Caenorhabditis elegans behavior during organophosphate exposure. 有机磷暴露时秀丽隐杆线虫行为调查的不同测定方法的比较分析。
microPublication biology Pub Date : 2025-05-16 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001533
Johanna Haszczyn, Vincent O'Connor, Lindy Holden-Dye, A Christopher Green, James Kearn
{"title":"A comparative analysis of distinct assays for the investigation of <i>Caenorhabditis elegans</i> behavior during organophosphate exposure.","authors":"Johanna Haszczyn, Vincent O'Connor, Lindy Holden-Dye, A Christopher Green, James Kearn","doi":"10.17912/micropub.biology.001533","DOIUrl":"10.17912/micropub.biology.001533","url":null,"abstract":"<p><p><i>C. elegans</i> motility is a convenient paradigm to describe the behavioral outcome of genetic- and drug-induced changes in neural circuits. Motility may be parameterized by scoring movement on solid medium or in liquid. In addition, body wall muscle contraction inhibits pharyngeal pumping, providing an indirect measure of motility. Here, the ability of these different experimental approaches to resolve organophosphate-related effects over time was investigated. In addition, two genetic mutations that alter neuromuscular function at the L-type body wall muscle were also investigated using these assays. This work highlights the benefits and limitations of distinct screening approaches for <i>C. elegans</i> behavior when analysing organophosphate mode of action on neuromuscular signalling. In particular, this work showed that pharyngeal pumping was able to resolve acute and chronic organophosphate-related effects, however liquid-based assays were best suited to resolve the phenotype of the genetic mutant L-AChR ( <i>ufis6).</i></p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The cadherin domains and the kinesin-binding intracellular domain of CASY-1/calsyntenin function in a redundant manner for learning. CASY-1/calsyntenin的钙粘蛋白结构域和运动蛋白结合的细胞内结构域在学习中以冗余的方式起作用。
microPublication biology Pub Date : 2025-05-16 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001600
Hayao Ohno, Yuzuha Komachiya, Yuichi Iino
{"title":"The cadherin domains and the kinesin-binding intracellular domain of CASY-1/calsyntenin function in a redundant manner for learning.","authors":"Hayao Ohno, Yuzuha Komachiya, Yuichi Iino","doi":"10.17912/micropub.biology.001600","DOIUrl":"10.17912/micropub.biology.001600","url":null,"abstract":"<p><p>Taste avoidance learning in <i>Caenorhabditis elegans</i> is regulated by the calsyntenin/alcadein homolog CASY-1 , which transports the insulin receptor DAF-2c to the synaptic region. This transport involves binding of the CASY-1 intracellular domain to the kinesin-1 (KIF5) complex. However, a previous study showed that the intracellular domain of CASY-1 is dispensable for learning. To investigate how CASY-1 functions, we performed functional domain mapping of CASY-1 . Both the cadherin domains of CASY-1 and its binding to kinesin-1 are individually dispensable, while simultaneous loss of both abolished the CASY-1 function, suggesting that CASY-1 enables robust intracellular transport through physical interactions with multiple proteins.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Suppression of har-1/CHCHD10 phenotypes for ALS-FTD therapy discovery. 抑制har-1/CHCHD10表型对ALS-FTD治疗的发现
microPublication biology Pub Date : 2025-05-15 eCollection Date: 2025-01-01 DOI: 10.17912/micropub.biology.001598
Audrey Labarre, Ericka Guitard, Gilles Tossing, J Alex Parker
{"title":"Suppression of <i>har-1/CHCHD10</i> phenotypes for ALS-FTD therapy discovery.","authors":"Audrey Labarre, Ericka Guitard, Gilles Tossing, J Alex Parker","doi":"10.17912/micropub.biology.001598","DOIUrl":"10.17912/micropub.biology.001598","url":null,"abstract":"<p><p>Mutations in <i>CHCHD10</i> are linked to a variety of neurodegenerative diseases, including amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD). The <i>Caenorhabditis elegans</i> orthologue of <i>CHCHD10</i> is <i>har-1 ,</i> and we investigated whether <i>har-1</i> mutants could be used for therapeutic discovery in ALS-FTD. Our results show that the small molecule pioglitazone and the probiotic <i>Lacticaseibacillus rhamnosus</i> HA-114 can alleviate <i>har-1</i> mutant phenotypes. These findings suggest that <i>har-1</i> mutants are suitable for modifier screens and could be adapted for high-throughput drug screening and microbiome studies to aid in discovering therapies for ALS-FTD.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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