A Tetracycline-Inducible Promoter Replacement System for Schizosaccharomyces pombe.

Abstract

Inducible promoters are essential tools for regulating gene expression. In fission yeast, various inducible promoter systems have been developed over the years, aiding gene function studies. A key challenge with existing promoters is their high expression in the "off " state, with most systems showing only about a 10-fold difference between "on" and "off" conditions. A recent study introduced the PenotetS tetracycline promoter system, achieving nearly a 100-fold dynamic range. However, it was not designed to replace endogenous promoters. In this work, we have adapted the PenotetS system to enable the replacement of gene promoters directly at their endogenous genomic locations.