{"title":"Sequence Analysis and Modeling of the Repetitive Region of the Long Isoform of Clarinet/CLA-1.","authors":"Benjamin Hunt, Timothy Hunt, Zhao Xuan","doi":"10.17912/micropub.biology.001712","DOIUrl":null,"url":null,"abstract":"<p><p>The <i>C. elegans</i> active zone gene <i>cla-1</i> encodes three main isoforms. The long isoform, CLA-1 L, functions beyond regulating synaptic vesicle exocytosis, including synaptic vesicle clustering and endocytic sorting of a transmembrane autophagy protein. CLA-1 L contains a large N-terminal repetitive region. Sequence analysis indicates that this region is enriched with acidic residues and displays disordered structures interspersed with helical sections. While modeling a portion of the repetitive region revealed dynamic transitions between compact and less compact conformations, modeling the entire region suggests a predominantly extended structure. This study sheds light on how CLA-1 L carries out its diverse functions.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402934/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001712","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
The C. elegans active zone gene cla-1 encodes three main isoforms. The long isoform, CLA-1 L, functions beyond regulating synaptic vesicle exocytosis, including synaptic vesicle clustering and endocytic sorting of a transmembrane autophagy protein. CLA-1 L contains a large N-terminal repetitive region. Sequence analysis indicates that this region is enriched with acidic residues and displays disordered structures interspersed with helical sections. While modeling a portion of the repetitive region revealed dynamic transitions between compact and less compact conformations, modeling the entire region suggests a predominantly extended structure. This study sheds light on how CLA-1 L carries out its diverse functions.
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