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Pubertal development is unaffected in female Mecp2 -heterozygous mice. 雌性Mecp2杂合小鼠的青春期发育不受影响。
microPublication biology Pub Date : 2026-09-09 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002400
Skylar Fortich, Lily Demilio, Mariam Ceesay, Anabelle Clower, Billy You Bun Lau
{"title":"Pubertal development is unaffected in female <i>Mecp2</i> -heterozygous mice.","authors":"Skylar Fortich, Lily Demilio, Mariam Ceesay, Anabelle Clower, Billy You Bun Lau","doi":"10.17912/micropub.biology.002400","DOIUrl":"10.17912/micropub.biology.002400","url":null,"abstract":"<p><p>Rett syndrome (RTT) affects predominantly females and is characterized by regression after an initial period of typical development. Previously, we showed that while adolescent female <i>Mecp2</i> -heterozygous mice (Het, mouse model of RTT) perform pup retrieval comparably to wild-type littermate controls (WT), adult Het regress and become inefficient. Here, we analyzed the pubertal trajectory of naïve nulliparous WT and Het to test whether abnormal pubertal development contributes to this regression phenotype. We found no genotypic differences in pubertal onset nor estrous cyclicity during adolescence and adulthood. These findings further support the role of <i>Mecp2</i> in experience-dependent plasticity within sensory cortices.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149042510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seed-associated bacterial community structure and possible transmission to seedling in wasabi, Eutrema japonicum. 山葵种子相关细菌群落结构及其对幼苗的可能传播。
microPublication biology Pub Date : 2026-09-09 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002222
Kana Ohata, Sho Hirashima, Yuzuki Akahori, Haruki Kitano, Harunosuke Shoji, Susumu Hisamatsu, Yusuke Katai, Hisae Hirata, Masayoshi Hashimoto
{"title":"Seed-associated bacterial community structure and possible transmission to seedling in wasabi, <i>Eutrema japonicum</i>.","authors":"Kana Ohata, Sho Hirashima, Yuzuki Akahori, Haruki Kitano, Harunosuke Shoji, Susumu Hisamatsu, Yusuke Katai, Hisae Hirata, Masayoshi Hashimoto","doi":"10.17912/micropub.biology.002222","DOIUrl":"10.17912/micropub.biology.002222","url":null,"abstract":"<p><p>Seeds harbor microbial communities that contribute to enhancing the plant stress resilience. Using 16S rRNA gene profiling, this study characterized the bacterial microbiota of dry and pre-germinated seeds, and three compartments (soil, root, and leaf) from seedlings of wasabi ( <i>Eutrema japonicum</i> ). The seed-associated communities exhibited distinct characteristics in terms of the diversity metrics. SourceTracker analysis together with shared amplicon sequence variants (ASVs) between dry and pre-germinated seeds suggests that the seed microbiota may be partially transmitted to the seedling tissues. In particular, Burkholderiaceae among the seed core ASVs dominated the leaf-associated microbiota, suggesting preferential seed-to-leaf transmission in wasabi.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149042537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Workflow for Accurate Measurement of PDI Reductase Kinetics Using a Fluorescent Disulfide Substrate. 使用荧光二硫基物精确测量PDI还原酶动力学的工作流程。
microPublication biology Pub Date : 2026-09-08 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002334
Nathan Ponzar, Nicola Pozzi
{"title":"Workflow for Accurate Measurement of PDI Reductase Kinetics Using a Fluorescent Disulfide Substrate.","authors":"Nathan Ponzar, Nicola Pozzi","doi":"10.17912/micropub.biology.002334","DOIUrl":"10.17912/micropub.biology.002334","url":null,"abstract":"<p><p>Protein disulfide isomerase (PDI) is essential for oxidative protein folding, proteostasis, and redox regulation and has become a promising target for thromboinflammation, cancer, and neurodegenerative disorders. Fluorogenic disulfide substrates like Bodipy FL L-cystine (BDSS) are essential for mechanistic studies but have low affinity for PDI, necessitating high micromolar concentrations to measure Michaelis-Menten kinetics. These conditions can lead to optical artifacts, requiring development of correction methods to enable rigorous characterization of PDI inhibitors and allosteric modulators. Here, we present a simple workflow for correcting fluorescence quenching during BDSS reduction assays and provide an example of its utility with two allosteric modulators.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149042524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene model for the ortholog of Wnt6 in Drosophila cardini. 红果蝇Wnt6同源基因的基因模型。
microPublication biology Pub Date : 2026-09-04 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002337
Brenna Peruso, Pablo Chialvo, Clare Scott Chialvo
{"title":"Gene model for the ortholog of <i>Wnt6</i> in <i>Drosophila cardini</i>.","authors":"Brenna Peruso, Pablo Chialvo, Clare Scott Chialvo","doi":"10.17912/micropub.biology.002337","DOIUrl":"10.17912/micropub.biology.002337","url":null,"abstract":"<p><p>We developed a gene model for the <i>Wnt oncogene analog 6</i> ortholog ( <i>Wnt6</i> ) in the ASM1890373v1 Genome Assembly (GenBank Accession: GCA_018903735.1 ) of <i>Drosophila cardini</i> . This ortholog was characterized as part of a developing dataset for a comparative study of detoxification gene family evolution in the <i>immigrans</i> - <i>tripunctata</i> radiation of the genus <i>Drosophila</i> using an adapted Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149009494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Against All Odds: Computational Screening Via Machine Learning Ranking and Generation of Antibody Candidates for Creutzfeldt-Jakob Disease. 排除万难：通过机器学习排序和生成克雅氏病候选抗体的计算筛选。
microPublication biology Pub Date : 2026-09-04 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002204
Vinayan Tiruvellore, Mike Koegle
{"title":"Against All Odds: Computational Screening Via Machine Learning Ranking and Generation of Antibody Candidates for Creutzfeldt-Jakob Disease.","authors":"Vinayan Tiruvellore, Mike Koegle","doi":"10.17912/micropub.biology.002204","DOIUrl":"10.17912/micropub.biology.002204","url":null,"abstract":"<p><p>Creutzfeldt-Jakob disease (CJD) is a fatal prion disorder with no approved treatments. This study developed a machine learning pipeline trained on 21 literature-curated PrP-targeting CDR sequences to rank 29,574 original and mutation-generated candidate sequences using neutralization, selectivity, and literature-based blood-brain barrier (BBB) proxy scores. The models showed internal performance (neutralization AUC = 0.9239; selectivity AUC = 0.7759), identified 10 high-percentile candidates, and generated 7,309 novel variants. These findings support hypothesis-generating sequence-level prioritization of PrP-targeting candidates, but do not establish native PrP <sup>Sc</sup> -specific binding, full-antibody efficacy, exact PrP epitope recognition, or in vivo BBB penetration and require further experimental validation and testing.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149009479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Early leaf senescence observed in the pen1pen3 double mutant is not dependent on HIR2, a protein that physically interacts with PEN1. 在pen1pen3双突变体中观察到的早期叶片衰老不依赖于HIR2，这是一种与PEN1物理相互作用的蛋白质。
microPublication biology Pub Date : 2026-09-04 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002392
Tina A Nguyen, David A Soriano, Judy A Brusslan
{"title":"Early leaf senescence observed in the <i>pen1pen3</i> double mutant is not dependent on HIR2, a protein that physically interacts with PEN1.","authors":"Tina A Nguyen, David A Soriano, Judy A Brusslan","doi":"10.17912/micropub.biology.002392","DOIUrl":"10.17912/micropub.biology.002392","url":null,"abstract":"<p><p>The <i>pen1pen3</i> double mutant displays early leaf senescence that depends on salicylic acid biosynthesis, a phytohormone associated with defense and leaf senescence. PEN1, a Qa SNARE, physically interacts with HIR2, a protein that tethers immune receptors to plasma membrane nanodomains. We hypothesized that PEN1 binding to HIR2 may prevent immune receptor localization. Consistent with this hypothesis is the expectation that <i>pen1pen3hir2</i> mutants would show reduced early leaf senescence when compared to <i>pen1pen3</i> . We constructed the triple mutant, quantified leaf senescence, and observed early leaf senescence that was not significantly different than <i>pen1pen3</i> . This result does not support our hypothesis.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149009450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Water nutrient concentration and temperature control microbial-driven decomposition of leaf litter in oceanic island streams. 海水养分浓度和温度控制海洋岛屿溪流中微生物驱动的凋落叶分解。
microPublication biology Pub Date : 2026-09-02 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002314
Verónica Ferreira, Pedro M Raposeiro, Vítor Gonçalves
{"title":"Water nutrient concentration and temperature control microbial-driven decomposition of leaf litter in oceanic island streams.","authors":"Verónica Ferreira, Pedro M Raposeiro, Vítor Gonçalves","doi":"10.17912/micropub.biology.002314","DOIUrl":"https://doi.org/10.17912/micropub.biology.002314","url":null,"abstract":"<p><p>Litter decomposition fuels food webs in forest streams. Thus, understanding the effects of water nutrients and temperature on litter decomposition and decomposers is key for predicting stream functioning under environmental change. We incubated leaf litter of woody species in streams to assess the effects of nutrient concentrations and temperature on microbial-driven litter decomposition and microbial activities. The relationship between biotic and abiotic variables was generally best described by a quadratic model: there was a hump-shaped relationship between biotic variables and nitrate concentrations and a U-shaped relationship between biotic variables and temperature. The stimulatory effect of high nutrient availability was limited by low temperature, while high nutrient availability alleviated low-temperature effects on litter decomposition and decomposers.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13579385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148992824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A fixation-weighted null model for the proportion of sex chromosome-autosome fusions. 性染色体-常染色体融合比例的固定加权零模型。
microPublication biology Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002109
Sarah Schmalz, Heath Blackmon
{"title":"A fixation-weighted null model for the proportion of sex chromosome-autosome fusions.","authors":"Sarah Schmalz, Heath Blackmon","doi":"10.17912/micropub.biology.002109","DOIUrl":"10.17912/micropub.biology.002109","url":null,"abstract":"<p><p>Recent null models for sex-autosome fusions have often relied on occurrence probability alone. However, evolutionary expectations must account for fixation probability. Under neutrality, fixation probability is inversely proportional to chromosomal population copy numbers, which vary across autosomes, X chromosomes, and Y chromosomes. We extend the original fusion model by weighting each fusion class by its relative neutral fixation probability. This updated model predicts substantially higher proportions of fixed SA-fusions, establishing a more biologically realistic null hypothesis. We provide an updated R function in the R package evobiR and demonstrate its impact by re-evaluating empirical fusion patterns in <i>Drosophila</i> and <i>Habronattus</i> .</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148979175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
miR-10-3p, but not miR-1006-3p, regulates mei-P26 expression in the Drosophila testis. miR-10-3p，而不是miR-1006-3p，在果蝇睾丸中调节mei-P26的表达。
microPublication biology Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002187
Shallinie Thangadurai, Elena Pak, Isabella Hammock, Alexander Murashov
{"title":"miR-10-3p, but not miR-1006-3p, regulates <i>mei-P26</i> expression in the <i>Drosophila</i> testis.","authors":"Shallinie Thangadurai, Elena Pak, Isabella Hammock, Alexander Murashov","doi":"10.17912/micropub.biology.002187","DOIUrl":"10.17912/micropub.biology.002187","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression, yet predicted miRNA target interactions often require experimental validation. TargetScan Fly predicts that both miR-10-3p and miR-1006-3p target the <i>Drosophila</i> RNA-binding protein Mei-P26, a critical regulator of germline development and miRNA biogenesis. Here, we experimentally demonstrate that overexpression of miR-10-3p is sufficient to significantly suppress <i>mei-P26</i> mRNA level in the testis. Consistently, inhibition of miR-10 using sponge increased mei-P26 positive spermatocytes in testis. Together, these findings suggest that miR-10-3p, but not miR-1006-3p, functionally regulates <i>mei-P26</i> in <i>Drosophila</i> testis, highlighting selective engagement of canonical miRNA pathways in <i>mei-P26</i> regulation.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148979179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Whole genome sequencing and phenotypic characterization of microbial isolates associated with two Floridian fig species. 与两个佛罗里达无花果物种相关的微生物分离株的全基因组测序和表型特征。
microPublication biology Pub Date : 2026-08-31 eCollection Date: 2026-01-01 DOI: 10.17912/micropub.biology.002288
Isabella Hall, Jenna Naumann, Shelby G Rinderer, Ally J Whitson, Pamela J B Brown, Justin Van Goor
{"title":"Whole genome sequencing and phenotypic characterization of microbial isolates associated with two Floridian fig species.","authors":"Isabella Hall, Jenna Naumann, Shelby G Rinderer, Ally J Whitson, Pamela J B Brown, Justin Van Goor","doi":"10.17912/micropub.biology.002288","DOIUrl":"10.17912/micropub.biology.002288","url":null,"abstract":"<p><p>Fig-associated microbes are increasingly recognized as important contributors to the ecology of fig ( <i>Ficus</i> ) communities, yet few cultured isolates are available for functional investigation. Students enrolled in a course-based undergraduate research experience (CURE) phenotypically characterized and generated high-quality genome assemblies for 22 bacterial isolates recovered from <i>Ficus aurea</i> and <i>Ficus citrifolia</i> in southern Florida, USA. These isolates represent six bacterial species spanning four genera and provide a genomic resource for future investigations of microbial interactions within fig communities.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2026 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148978113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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