C. albicans pseudouridine glycosidase is important for growth in hygromycin stress and for filamentation.

Abstract

When an RNA is no longer needed or has become damaged, it is degraded to its single base components. Pseudouridine is found in all domains of life and is found in a variety of types of RNA. Pseudouridine has ribose and uracil moieties attached via a C-C bond. Cleavage of this bond is performed by pseudouridine glycosidases. We find deletion of the pseudouridine glycosidase from the human fungal pathogen C. albicans leads to sensitivity to hygromycin and changes to filamentation. In addition, deletion of pseudouridine glycosidase leads to the upregulation of several permeases. Our data suggest pseudouridine glycosidases are important for C. albicans fitness.