Leif R Neitzel, Hussain F Basrai, Charles C Hong, Charles H Williams
{"title":"Ogremorphin抑制GPR68介导的MUC5AC表达。","authors":"Leif R Neitzel, Hussain F Basrai, Charles C Hong, Charles H Williams","doi":"10.17912/micropub.biology.001639","DOIUrl":null,"url":null,"abstract":"<p><p>MUC5AC hypersecretion exacerbates airway obstruction in lung diseases, such as asthma, and promotes tumor growth. The expression of MUC5AC is driven by the pH-sensing receptor, GPR68. Herein we demonstrate that acidification (pH 6.4) increased MUC5AC mRNA and protein expression in a GPR68-dependent manner in A549 cells. Treatment with Ogremorphin (OGM), a specific GPR68 inhibitor, reduced MUC5AC mRNA and protein expression irrespective of pH. These findings were confirmed by shRNA-mediated knockdown of GPR68. These data suggest GPR68 inhibition by OGM as a potential therapy for mucus hypersecretion to improve airway clearance and reduce tumor progression in lung diseases.</p>","PeriodicalId":74192,"journal":{"name":"microPublication biology","volume":"2025 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489611/pdf/","citationCount":"0","resultStr":"{\"title\":\"Ogremorphin inhibits GPR68 mediated MUC5AC expression.\",\"authors\":\"Leif R Neitzel, Hussain F Basrai, Charles C Hong, Charles H Williams\",\"doi\":\"10.17912/micropub.biology.001639\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>MUC5AC hypersecretion exacerbates airway obstruction in lung diseases, such as asthma, and promotes tumor growth. The expression of MUC5AC is driven by the pH-sensing receptor, GPR68. Herein we demonstrate that acidification (pH 6.4) increased MUC5AC mRNA and protein expression in a GPR68-dependent manner in A549 cells. Treatment with Ogremorphin (OGM), a specific GPR68 inhibitor, reduced MUC5AC mRNA and protein expression irrespective of pH. These findings were confirmed by shRNA-mediated knockdown of GPR68. These data suggest GPR68 inhibition by OGM as a potential therapy for mucus hypersecretion to improve airway clearance and reduce tumor progression in lung diseases.</p>\",\"PeriodicalId\":74192,\"journal\":{\"name\":\"microPublication biology\",\"volume\":\"2025 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12489611/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"microPublication biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17912/micropub.biology.001639\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"microPublication biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17912/micropub.biology.001639","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
MUC5AC hypersecretion exacerbates airway obstruction in lung diseases, such as asthma, and promotes tumor growth. The expression of MUC5AC is driven by the pH-sensing receptor, GPR68. Herein we demonstrate that acidification (pH 6.4) increased MUC5AC mRNA and protein expression in a GPR68-dependent manner in A549 cells. Treatment with Ogremorphin (OGM), a specific GPR68 inhibitor, reduced MUC5AC mRNA and protein expression irrespective of pH. These findings were confirmed by shRNA-mediated knockdown of GPR68. These data suggest GPR68 inhibition by OGM as a potential therapy for mucus hypersecretion to improve airway clearance and reduce tumor progression in lung diseases.
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