Joanna A Mas, Chase E Cristella, Vu Hao M N Phan, Lillian S Wendt, Charlotte A Rose, Abigail Ali, David F Carpio, Christine Cole, Paige Embley, Jack E Hoskins-Harris, Delia Johnson, Noelle Ledoux, Hannah W Lwin, Sarah Salah, Erin Weisbart, Stacey J Criswell, Omar Alberto Quintero-Carmona
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引用次数: 0
摘要
肌球蛋白- x (MYO10)是一种基于肌动蛋白的运动蛋白,参与细胞骨架动力学、膜相互作用和整合素介导的粘附。为了研究MYO10在细胞中的作用,我们使用慢病毒shRNA生成了MYO10敲低(MYO10 KD) HeLa和COS7细胞系。与野生型细胞相比,两种MYO10 KD细胞系在伤口试验中均表现出增殖减少和细胞迁移受损。此外,HeLa细胞的边缘丝状足较少。此外,MYO10 KD细胞在层粘连蛋白包被的底物上扩散增加,表明整合素激活和细胞骨架连锁发生了改变。我们的研究结果强化了MYO10在细胞增殖、粘附和迁移中的重要性。这些MYO10 KD系为今后MYO10的研究提供了一种可行的细胞培养模型。
Cells stably expressing shRNA against MYO10 display altered cell motility.
Myosin-X (MYO10) is an actin-based motor protein involved in cytoskeletal dynamics, membrane interactions, and integrin-mediated adhesion. To investigate MYO10's cellular roles, we generated MYO10 knockdown (MYO10 KD ) HeLa and COS7 cell lines using lentiviral shRNA. Compared to wild-type cells, both MYO10 KD lines showed reduced proliferation and impaired cell migration in wound assays. Additionally, there were fewer edge filopodia in HeLa cells. Furthermore, MYO10 KD cells demonstrated increased spreading on laminin-coated substrates, suggesting altered integrin activation and cytoskeletal linkage. Our results reinforce MYO10's importance in cell proliferation, adhesion, and migration. These MYO10 KD lines provide an accessible cell culture model for future study of MYO10.