Cells stably expressing shRNA against MYO10 display altered cell motility.

microPublication biology Pub Date : 2025-09-19 eCollection Date: 2025-01-01 DOI:10.17912/micropub.biology.001764

Joanna A Mas, Chase E Cristella, Vu Hao M N Phan, Lillian S Wendt, Charlotte A Rose, Abigail Ali, David F Carpio, Christine Cole, Paige Embley, Jack E Hoskins-Harris, Delia Johnson, Noelle Ledoux, Hannah W Lwin, Sarah Salah, Erin Weisbart, Stacey J Criswell, Omar Alberto Quintero-Carmona

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Abstract

Myosin-X (MYO10) is an actin-based motor protein involved in cytoskeletal dynamics, membrane interactions, and integrin-mediated adhesion. To investigate MYO10's cellular roles, we generated MYO10 knockdown (MYO10 ^KD ) HeLa and COS7 cell lines using lentiviral shRNA. Compared to wild-type cells, both MYO10 ^KD lines showed reduced proliferation and impaired cell migration in wound assays. Additionally, there were fewer edge filopodia in HeLa cells. Furthermore, MYO10 ^KD cells demonstrated increased spreading on laminin-coated substrates, suggesting altered integrin activation and cytoskeletal linkage. Our results reinforce MYO10's importance in cell proliferation, adhesion, and migration. These MYO10 ^KD lines provide an accessible cell culture model for future study of MYO10.

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稳定表达shRNA对抗MYO10的细胞表现出改变的细胞运动性。

肌球蛋白- x （MYO10）是一种基于肌动蛋白的运动蛋白，参与细胞骨架动力学、膜相互作用和整合素介导的粘附。为了研究MYO10在细胞中的作用，我们使用慢病毒shRNA生成了MYO10敲低（MYO10 KD） HeLa和COS7细胞系。与野生型细胞相比，两种MYO10 KD细胞系在伤口试验中均表现出增殖减少和细胞迁移受损。此外，HeLa细胞的边缘丝状足较少。此外，MYO10 KD细胞在层粘连蛋白包被的底物上扩散增加，表明整合素激活和细胞骨架连锁发生了改变。我们的研究结果强化了MYO10在细胞增殖、粘附和迁移中的重要性。这些MYO10 KD系为今后MYO10的研究提供了一种可行的细胞培养模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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microPublication biology

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3 weeks