Frontiers in dental medicine最新文献

{"title":"A three-year prospective cohort study evaluating implant stability utilising the osstell® and periotest™ devices","authors":"I. Reynolds, L. Winning, I. Polyzois","doi":"10.3389/fdmed.2023.1139407","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1139407","url":null,"abstract":"Objectives To investigate implant stability measurements from two different devices and at three different time points in order to determine their level of correlation. To also evaluate the influence of a range of clinical characteristics on the values produced by the devices at these three time points. Materials & Methods Measurements were recorded at implant placement (T1), implant exposure (T2) and at 3 years from implant placement (T3). A range of clinical data was collected including patient demographics and site characteristics. Stability measurements and clinical characteristics were recorded for 29 patients and 68 dental implants at T1, subsequent stability measurements were recorded for 67 implants at T2 and 58 implants at T3. Correlation testing between the Osstell® and Periotest™ devices was carried out utilising Spearman's rank correlation for each time point. Analysis of the difference between clinical factors and stability measurements was compared using Krushal-Wallis test for each variable and time point. Results A single dental implant failed shortly after 2nd stage surgery for an overall survival rate of 98% during the study timeline. The median ISQ value was 73.25 (IQR 67–75) at T1 and 74 (IQR 70.5–77) at T3. The median Periotest value was −4 (IQR −6, −2) at T1 and −6 (IQR −7, −5) at T3. The range of ISQ values observed was 50 (39–89) ISQ at T1 and decreased to 21 (61–82) ISQ at T3. The Periotest values ranged from 37 (29 to −8) at T1 and decreased to 6 (−2 to −8) at T3. A weak to moderate correlation was observed between mean ISQ and Periotest values across time-points T1, T2 and T3, (r = −0.26, p = 0.05), (r = −0.35, p < 0.01) and (r = −0.28, p = 0.04) respectively. Conclusions Based on the results of this study there was a weak to moderate level of correlation between values recorded between the two measurement devices at implant placement, implant exposure and three years following placement. For both the Osstell® and Periotest™ a narrowing of the range of stability values was observed from T1 to T3. In general, Periotest™ seemed to be more sensitive in highlighting differences in measurements affected by local conditions.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46954985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ErbB- and MUC1-targetted CAR-T cell immunotherapy of oral squamous cell carcinoma","authors":"Sa Summers, V. Salih, A. Foey","doi":"10.3389/fdmed.2023.1116402","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1116402","url":null,"abstract":"Chimeric antigen receptor T (CAR-T) cell therapy has shown great success in treating B cell malignancies however, there are many challenges which limit their therapeutic efficacy in solid tumours. Immunotherapy of head and neck squamous cell carcinoma (HNSCC), and in particular, oral squamous cell carcinoma (OSCC), presents a unique set of challenges including lack of consistently expressed tumour associated antigens (TAAs) and the immunosuppressive tumour microenvironment (TME). Currently, there are few clinical trials investigating the use of CAR-T cells in HNSCC/OSCC however results from trials investigating similar solid tumours, such as breast cancer, can be adopted to help evaluate the use of CAR-T in this cancer. In this review, the process of CAR-T cell engineering, and different generations of these cells will be summarised, highlighting their potential use in treating HNSCC through targeting ErbB and MUC1; TAAs highly expressed by this solid tumour. Potential strategies including combination therapy, utilising both TAA-targeting CAR-Ts and immune checkpoint inhibitors, such as PD-L1, has been discussed, in an attempt to develop synergistic anti-tumour responses. In addition to this, the use of dual-targeting CAR-T cells, synthetic NOTCH (synNOTCH) receptors and alternative non-tumour targets of the TME have been reviewed. Such combination therapies have been shown to help limit solid tumour progression and enhance both the safety and efficacy of CAR-T cell immunotherapy, which may be adopted for the treatment and management of OSCC.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45417162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The RGD region of bone sialoprotein affects metabolic activity in mice","authors":"K. Nagasaki, Atsuhiro Nagasaki, J. Taylor, B. Kear, Yinyan Ma, M. Somerman, O. Gavrilova","doi":"10.3389/fdmed.2023.1124084","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1124084","url":null,"abstract":"Introduction Bone sialoprotein (BSP) is a key regulator of mineralized tissue formation. Previously, we generated BSP-KAE knock-in mice (KAEKI mice) by substituting a non-function KAE (lysine-alanine-glutamic acid) for the integrin-binding RGD (arginine-glycine-aspartic acid) sequence and reported a vital role of the BSP-RGD motif in modulating the periodontal ligament (PDL). Specifically, histologically a disorganization of the PDL was noted, resulting in a weakened function of the PDL as measured by dynamic mechanical analysis. Intriguingly, also noted was a weight gain as KAEKI mice aged. While several proteins associated with mineralized tissues are reported to affect energy metabolism, the metabolic role of the BSP-RGD region has yet to be elucidated. Here we focus on defining the role of the BSP-RGD region in metabolic activity. Methods Body weight, body composition, and caloric intake were measured in wild type (WT) and KAEKI mice. Energy expenditure was estimated using energy balance technique. Epididymal fat, interscapular fat, and liver were harvested for histological analysis. Systemic metabolic phenotype was assessed by sera analyses, insulin tolerance and glucose tolerance tests. Results The results showed that KAEKI mice developed mild obesity starting from 13 weeks postnatal (wpn). The increase in body weight correlated with an increase in lean mass and visceral adiposity. Histological examination revealed adipocyte hypertrophy in white epididymal fat and interscapular brown fat in KAEKI vs. WT mice at 17 wpn. Metabolic profiling indicated that KAEKI mice had dyslipidemia and hyperleptinemia but no significant changes in glucose metabolism. Energy balance analyses revealed that hyperphagia preceded weight gain in KAEKI mice. Conclusion These data suggest that the RGD region of BSP affects energy metabolism by regulating food intake, with further studies warranted to uncover the underlying mechanisms.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46178720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfabrication approaches for oral research and clinical dentistry","authors":"Paola Tiozzo-Lyon, Matías Andrade, C. Leiva-Sabadini, José Morales, Antonia Olivares, A. Ravasio, S. Aguayo","doi":"10.3389/fdmed.2023.1120394","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1120394","url":null,"abstract":"Currently, there is a variety of laboratory tools and strategies that have been developed to investigate in-vivo processes using in-vitro models. Amongst these, microfabrication represents a disruptive technology that is currently enabling next-generation biomedical research through the development of complex laboratory approaches (e.g., microfluidics), engineering of micrometer scale sensors and actuators (micropillars for traction force microscopy), and the creation of environments mimicking cell, tissue, and organ-specific contexts. Although microfabrication has been around for some time, its application in dental and oral research is still incipient. Nevertheless, in recent years multiple lines of research have emerged that use microfabrication-based approaches for the study of oral diseases and conditions with micro- and nano-scale sensitivities. Furthermore, many investigations are aiming to develop clinically relevant microfabrication-based applications for diagnostics, screening, and oral biomaterial manufacturing. Therefore, the objective of this review is to summarize the current application of microfabrication techniques in oral sciences, both in research and clinics, and to discuss possible future applications of these technologies for in-vitro studies and practical patient care. Initially, this review provides an overview of the most employed microfabrication methods utilized in biomedicine and dentistry. Subsequently, the use of micro- and nano-fabrication approaches in relevant fields of dental research such as endodontic and periodontal regeneration, biomaterials research, dental implantology, oral pathology, and biofilms was discussed. Finally, the current and future uses of microfabrication technology for clinical dentistry and how these approaches may soon be widely available in clinics for the diagnosis, prevention, and treatment of relevant pathologies are presented.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42484662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of junctional epithelium in maintaining dento-gingival adhesion and periodontal health","authors":"L. Theodoro, V. Garcia, E. Ervolino, J. Holcroft, C. McCulloch, B. Ganss","doi":"10.3389/fdmed.2023.1144537","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1144537","url":null,"abstract":"The dento-gingival junction comprises multiple epithelia including the junctional epithelium (JE), which is the most coronally-located structural element of the dento-gingival junction that demarcates external from internal periodontal environments. After tooth eruption into the oral cavity, a specialized basal lamina is formed that provides firm attachment of the JE to the enamel. This attachment prevents microbial species and oral debris from entering subjacent periodontal tissues. Here we discuss the expression of certain JE adhesion molecules and enamel proteins that maintain the health of the dento-gingival junction but that are perturbed in the pathogenesis of periodontitis. We also consider how evolutionary processes have influenced the development of the JE as a specialized adhesion that is well-suited for protection of the dento-gingival junction. A detailed understanding of the biology of the JE will deepen current models of dento-gingival adhesion, potentially clarify inter-patient variability of susceptibility to periodontitis and help to identify new roles of enamel proteins in periodontal regeneration.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45502329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal regeneration: Lessons from the periodontal ligament-cementum junction in diverse animal models","authors":"E. Sone, C. McCulloch","doi":"10.3389/fdmed.2023.1124968","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1124968","url":null,"abstract":"The attachment of the roots of mammalian teeth of limited eruption to the jawbone is reliant in part on the mineralization of collagen fibrils of the periodontal ligament (PDL) at their entry into bone and cementum as Sharpey's fibers. In periodontitis, a high prevalence infection of periodontal tissues, the attachment apparatus of PDL to the tooth root is progressively destroyed. Despite the pervasiveness of periodontitis and its attendant health care costs, and regardless of decades of research into various possible treatments, reliable restoration of periodontal attachment after surgery is not achievable. Notably, treatment outcomes in animal studies have often demonstrated more positive regenerative outcomes than human clinical studies. Conceivably, defining how species diversity affects cementogenesis and cementum/PDL regeneration could be instructive for informing novel and more efficacious treatment strategies. Here we briefly review differences in cementum and PDL attachment in commonly used animal models to consider how species differences may lead to enhanced regenerative outcomes.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45917794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-curing dental resin-based composites: How it works and how you can make it work","authors":"D. Watts","doi":"10.3389/fdmed.2023.1108316","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1108316","url":null,"abstract":"Aim Clinicians may become quite familiar with the rapid transformation of composite pastes to rigid solids, as a routine phenomenon in operative dentistry. But they may still lack scientific understanding of how and why this happens. Efforts to learn scientifically about the interaction between light beams and resin-composites can significantly promote effective clinical placement of restorations. Neglect of such study can result in practical procedures of light-curing that are inadequate or even seriously defective. Method This review addresses the underlying science and technology to elucidate how light curing works, for dental resin-based composites, including—but not limited to—bulk fill types. This involves questions concerning: (a) the particle-wave understanding of light; (b) how photons can penetrate sufficiently deeply into bulk fill composites; (c) the necessary technology of LED light-curing units (LCUs); (d) the criteria for absorption of photons by photo initiators to initiate free-radical addition polymerisation. Conclusions The implications for clinical practice are surveyed. These include design variables and selection criteria for LED-LCUs and guidelines on their use. This is to guide practitioners towards safe and effective light-curing procedures so that they can achieve optimal result for their patients.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49206524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-activated irrigation: Cavitation and streaming effects from dental lasers","authors":"C. I. Anton y Otero, L. Marger, E. di Bella, A. Feilzer, I. Krejci","doi":"10.3389/fdmed.2022.1010916","DOIUrl":"https://doi.org/10.3389/fdmed.2022.1010916","url":null,"abstract":"Introduction The aim of the present study was to assess the generation of laser-activated irrigation (LAI) with a 9,300-nm CO2 laser, a 455- and 970-nm diode laser as well as an 810- and 980-nm diode laser with and without carbon-coated “hot-tips” for improved irrigation in endodontics. Therefore, the formation of cavitation bubbles at the laser tip-ends in different liquids was evaluated and compared to the traditionally applied Er:YAG laser. Methods The CO2 laser was applied at different power settings at a repetition rate of 14 Hz, SIROLASE for each wavelength at 10% Duty Cycle, and WISER with both wavelengths at different power settings and wavelengths. The LAI was videotaped with a high-speed camera. To measure the streaming velocity due to cavitation, all lasers were loosely coated with carbon particles and activated in pure water. Then, due to LAI, moving particles were videotaped with the same high-speed camera set-up. To determine the streaming velocities, the movement of one carbon particle was tracked with the help of a measuring tool in ImageJP software and streaming velocities were calculated. Results With both diode laser devices at four wavelengths, it was only possible to introduce clear cavitation bubbles with low-power parameters (180 mW for the SIROLASE and 200 mW average power for the WISER laser), with the black coating of the laser tips. The 9,300-nm CO2 laser produced clear cavitation bubbles with all tested parameters. Consequently, all tested lasers produced streaming of the liquid, with decreasing velocities at increasing distances from the laser tip: Er:YAG laser 6.4 m/s, CO2 laser up to 5 m/s, and diode lasers in the range of 0.01–0.09 m/s. LIA with the Er:YAG and 9,300-nm CO2 laser generated high and similar streaming velocities. The diode lasers tested generated significantly lower streaming velocities.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46609740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xerogenic medications may contribute to decreased unstimulated salivary flow in patients with oral burning and/or gastro-esophageal reflux disease","authors":"L. Sangalli, Walied Eldomiaty, Craig S. Miller","doi":"10.3389/fdmed.2023.1047235","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1047235","url":null,"abstract":"Background Patients who complain of mucosal burning sensations (i.e., glossodynia and gastro-esophageal reflux disease, GERD) often take multiple medications. However, the relationship between xerogenic medication intake and salivary flow in these patients has not been thoroughly examined. Methods A retrospective study of 192 consecutive patients diagnosed with glossodynia (ICD-10-CM) at a regional center over a six-year period was performed. Data from electronic health records were extracted and relationships between medication intake, unstimulated whole salivary flow rate (UWSFR), xerostomia and GERD were determined by chi-square, t-test, and correlation analysis. Results Of 134 records that met inclusion criteria, 87.1% of patients reported daily intake of one or more xerogenic medications. Two or more xerogenic medications were taken significantly more often by patients with glossodynia reporting GERD than those with glossodynia without GERD (p = .02). UWSFR was negatively correlated with number of medications [r(103) = −.277, p = .005] and xerogenic medications [r(103) = −.195, p = .049]. The lowest UWSFR was observed with use of trazodone and cyclobenzaprine. Conclusions Daily xerogenic medication intake, hyposalivation, and xerostomia were commonly present and potentially interrelated in patients who suffer from glossodynia and/or GERD. Practical implications Clinicians should be aware of the consequences of prescribing multiple and certain xerogenic medications in reducing UWSFR, especially in patients physiologically at risk of hyposalivation such as those suffering from GERD and/or glossodynia.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45498235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Frontiers in dental medicine: Highlights in dental materials 2021/22","authors":"Josette Camilleri","doi":"10.3389/fdmed.2023.1152825","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1152825","url":null,"abstract":"COPYRIGHT © 2023 Camilleri. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48575371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}