Frontiers in dental medicine最新文献

{"title":"Temperature-responsive PCL-PLLA nanofibrous tissue engineering scaffolds with memorized porous microstructure recovery","authors":"Seth M. Woodbury, W. Benton Swanson, Lindsey Douglas, David Niemann, Yuji Mishina","doi":"10.3389/fdmed.2023.1240397","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1240397","url":null,"abstract":"Biomaterial scaffolds in tissue engineering facilitate tissue regeneration and integration with the host. Poor healing outcomes arise from lack of cell and tissue infiltration, and ill-fitting interfaces between matrices or grafts, resulting in fibrous tissue formation, inflammation, and resorption. Existing tissue engineering scaffolds struggle to recover from deformation to fit irregularly shaped defects encountered in clinical settings without compromising their mechanical properties and favorable internal architecture. This study introduces a synthetic biomaterial scaffold composed of high molecular weight poly (L-lactic acid) (PLLA) and an interpenetrating network of poly (ε-caprolactone) (PCL), in a composition aiming to address the need for conformal fitting synthetic matrices which retain and recover their advantageous morphologies. The scaffold, known as thermosensitive memorized microstructure (TS-MMS), forms nanofibrous materials with memorized microstructures capable of recovery after deformation, including macropores and nanofibers. TS-MMS nanofibers, with 50–500 nm diameters, are formed via thermally induced phase separation (TIPS) of PLLA after in situ polymerization of PCL-diacrylate. A critical partial-melting temperature of TS-MMS at 52°C enables bulk deformation above this temperature, while retaining the nanofibrous and macroporous structures upon cooling to 37°C. Incorporation of drug-loaded poly (lactide-co-glycolide) (PLGA) nanoparticles directly into TS-MMS nanofibers during fabrication allows sustained release of a model drug for up to 40 days. Subcutaneous implantation in vivo using LysM-Cre;td-Tomato; Col1eGFP mice demonstrates successful cellularization and integration of deformed/recovered TS-MMS materials, surpassing the limitations of deformed PLLA scaffolds, to facilitate cell and vasculature infiltration requisite for successful bone regeneration. Additionally we demonstrated a method for embedding controlled release vehicles directly into the scaffold nanofibers; controlled release of simvastatin enhances vascularization and tissue maturation. TS-MMS scaffolds offer promising improvements in clinical handling and performance compared to existing biomaterial scaffolds.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135719447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A perspective: Regeneration of soft and hard tissues in the oral cavity, from research to clinical practice","authors":"Israel Puterman, Matthew J. Fien, Juan Mesquida, Ignacio Ginebreda, Guillermo Bauza, Martha Somerman","doi":"10.3389/fdmed.2023.1242547","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1242547","url":null,"abstract":"Regenerative medicine has gained much attention and has been a hot topic in all medical fields since its inception, and dentistry is no exception. However, innovations and developments in basic research are sometimes disconnected from daily clinical practice. This existing gap between basic research and clinical practice can only be addressed with improved communication between clinicians, academicians, industry, and researchers to facilitate the advance of evidence-based therapies and procedures and to direct research to areas of clinical need. In this perspective, six participants with strong clinical and research interests debated five previously conceived questions. These questions covered current methods and procedures for soft and hard tissue regeneration in the oral cavity with predictable outcomes, limitations of their respective protocols, and needs for future development of regenerative materials and technologies.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135016847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there still a need for dental textbooks when doubts can be clarified by artificial intelligence and open access science?","authors":"João Paulo Mendes Tribst, Amanda Maria de Oliveira Dal Piva, Cornelis J Kleverlaan, Albert J Feilzer","doi":"10.3389/fdmed.2023.1236861","DOIUrl":"10.3389/fdmed.2023.1236861","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1236861"},"PeriodicalIF":1.5,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43869301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allocating intricacies: pediatric oral health spotlight in the union health and well-being budget of India.","authors":"Vaibhav Kumar, Rushikesh Sangle, Romi Jain, Nikhil Bhanushali, Sakshi Yadav, Ayesha Qureshi, Harshal Tandel, Pranjal Mhatre","doi":"10.3389/fdmed.2023.1134294","DOIUrl":"10.3389/fdmed.2023.1134294","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1134294"},"PeriodicalIF":1.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45908764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Applied biophysics of the tooth and dental materials.","authors":"Sebastian Aguayo, Nelson P Barrera, Laurent Bozec","doi":"10.3389/fdmed.2023.1258974","DOIUrl":"10.3389/fdmed.2023.1258974","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1258974"},"PeriodicalIF":1.5,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43461478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactobacillus acidophilus</i> impairs the establishment of pathogens in a subgingival multispecies biofilm.","authors":"Manuela Rocha Bueno, Gustavo Dudu-Silva, Tatiane Tiemi Macedo, Aline Paim de Abreu Paulo Gomes, Arthur Rodrigues Oliveira Braga, Lucas Daylor Aguiar Silva, Bruno Bueno-Silva","doi":"10.3389/fdmed.2023.1212773","DOIUrl":"10.3389/fdmed.2023.1212773","url":null,"abstract":"<p><p>The present study evaluated the antibiofilm effects of <i>Lactobacillus acidophilus</i> within a subgingival multispecies biofilm. <i>Lactobacillus acidophilus</i> (La5) at 1 × 10², 1 × 10⁴, and 1 × 10⁶ were included at the beginning of biofilm formation, which lasted 7 days. The biofilms comprised 33 periodontitis-related bacterial species and the Calgary Biofilm device was used. At the end, DNA-DNA hybridization (checkerboard) was performed. A Kruskal-Wallis test followed by a Dunn <i>post hoc</i> test were performed (<i>p</i> ≤ 0.05). La5 at 1 × 10⁴ and 1 × 10⁶ reduced the total counts of biofilm and the proportions of red and green complexes when compared to the control biofilm without La5 (<i>p</i> ≤ 0.05). La5 at 1 × 10⁴ increased the proportions of <i>Actinomyces</i> complex compared to the controls (<i>p</i> ≤ 0.05). Both La5 at 1 × 10⁴ and 1 × 10⁶ decreased levels of 20 and 14 distinct species, respectively, including <i>Porphyromonas gingivalis</i>, <i>Prevotella intermedia</i>, <i>Fusobacterium nucleatum polymorphum</i>, and <i>Parvimonas micra</i> compared to the control (<i>p</i> ≤ 0.05). Only La5 at 1 × 10⁴ reduced the levels of <i>Tannerella forsythia</i>, <i>Fusobacterium periodonticum</i>, and <i>Aggregatibacter actinomycetencomytans</i> compared to the control (<i>p</i> ≤ 0.05). <i>L. acidophilus</i> inhibited establishing periodontic pathogens from red complex such as <i>P. gingivalis</i> and <i>T. forsythia</i> in a subgingival multispecies biofilm.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1212773"},"PeriodicalIF":1.5,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47140506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Advanced Platelet Rich Fibrin (A-PRF+), Enamel Matrix Derivative (EMD) and Open Flap Debridement on clinical and wound healing parameters in molar furcation sites: A case series from a RCT study.","authors":"L Pitzurra, D Vasdravellis, N A M Rosema, S Bizzarro, B G Loos","doi":"10.3389/fdmed.2023.1223217","DOIUrl":"10.3389/fdmed.2023.1223217","url":null,"abstract":"<p><strong>Aim: </strong>To study the effects of advanced platelet-rich fibrin (A-PRF+) and enamel matrix derivative (EMD) compared to open flap debridement (OFD) alone in molar furcation sites grade II on clinical and wound healing parameters.</p><p><strong>Materials and methods: </strong>A randomized controlled trial was designed. Eligible patients were randomly allocated to one of three treatment groups: A-PRF+, EMD or OFD. The patients and clinical examiners were blinded for the treatment received. A minimally invasive microsurgical approach was performed for the three modalities. Clinical measurements were scored at baseline and 6 months post-operatively. The clinical healing of each furcation was scored via the Early Wound Healing Index on day 3, 1 week, 2 weeks and 6 weeks.</p><p><strong>Results: </strong>17 patients (A-PRF+ <i>n</i> = 6, EMD <i>n</i> = 5, OFD <i>n</i> = 6) completed the 6 months of follow-up. The further completion of the trial had to be cancelled due to the COVID-19 pandemic. In three patients in the A-PRF+ group, the grade II of the treated furcation regressed to grade I; the corresponding number in the EMD and OFD groups was zero and one respectively. Further, 3, 1 and 4 patients in the PRF, EMD and OFD groups respectively, showed a gain of bone level ≥1 mm. The defects in the A-PRF+ group showed delayed early healing compared to the EMD and OFD groups.</p><p><strong>Conclusion: </strong>The case series (RCT design) suggests a slight advantage for A-PRF+ over EMD and OFD, regarding the regressing of a furcation II to grade I; however this treatment showed delayed early wound healing compared to EMD or OFD.</p><p><strong>Clinical trial registration: </strong>https://www.isrctn.com/, identifier ISRCTN13520922.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1223217"},"PeriodicalIF":1.5,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44405041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Bone and metabolic activities.","authors":"T E Van Dyke, N E Hatch, K Joshipura, N E Lane, M Murshed, F Nociti, B Olsen, M J Somerman","doi":"10.3389/fdmed.2023.1244033","DOIUrl":"10.3389/fdmed.2023.1244033","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1244033"},"PeriodicalIF":1.5,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44227757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An engineered biomaterial to harness the differentiation potential of endogenous human gingival mesenchymal stem cells (hGMSCs).","authors":"Mohammad Mahdi Hasani-Sadrabadi, Weihao Yuan, Sevda Sevari, Bo Yu, Sahar Ansari, Alireza Moshaverinia","doi":"10.3389/fdmed.2023.1235096","DOIUrl":"10.3389/fdmed.2023.1235096","url":null,"abstract":"<p><p>Here, we developed a stromal cell-derived factor-1a (SDF-1α) delivery biomaterial as an artificial polymeric-based niche with the ability to recruit local endogenous human gingival mesenchymal stem cells (hGMSCs) for craniofacial bone regeneration applications. Polydopamine-coated poly(ε-caprolactone) (PCL)-gelatin electrospun membranes were loaded with stromal cell-derived factor-1α (SDF-1α) via physical adsorption. Subsequently, the release profile of SDF-1α and the chemotactic capacity on human bone marrow mesenchymal stem cells (hBMMSCs) and hGMSCs were evaluated. The osteogenic differentiation capacity of the recruited MSCs was also assessed <i>in vitro</i>. Our results confirmed the sustainable release of SDF-1α from the developed biomaterial promoting the migration and homing of human bone marrow mesenchymal stem cells (hBMMSCs) and hGMSCs. Moreover, the results of the osteogenic differentiation assay showed that SDF-1α delivery significantly enhanced osteogenic differentiation of hBMMSCs and hGMSCs and up-regulated the gene expression of osteogenic markers compared to the control group. In conclusion, the current study successfully developed a novel and effective treatment modality for craniofacial bone regeneration by recruiting the autogenous progenitor cells including hGMSCs. The developed niches can potentially lead to the development of a novel platform for targeted manipulation of <i>in vivo</i> microenvironment to achieve efficient and safe craniofacial cell reprogramming, which also will pave the road to determine the capacity of local hGMSCs' contribution to <i>in situ</i> bone regeneration.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"1 1","pages":"1235096"},"PeriodicalIF":1.5,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41635453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia exerts disruptive effects on the secretion of TGF-β₁ and its matrix ligands, decorin and biglycan, by mesenchymal sub-populations and macrophages during bone repair.","authors":"Norhayati Yusop, Ryan Moseley, Rachel J Waddington","doi":"10.3389/fdmed.2023.1200122","DOIUrl":"10.3389/fdmed.2023.1200122","url":null,"abstract":"<p><strong>Introduction: </strong>Bone has a high capacity for repair, but for patients with uncontrolled type 2 diabetes mellitus (T2DM), the associated hyperglycemia can significantly delay osteogenic processes. These patients respond poorly to fracture repair and bone grafts, leading to lengthy care plans due to arising complications. Mesenchymal stromal cells (MSCs) and M2 macrophages are both major sources of transforming growth factor-β₁ (TGF-β₁), a recognized mediator for osteogenesis and whose bioavailability and activities are further regulated by matrix small leucine-rich proteoglycans (SLRPs), decorin and biglycan. The aim of this study was to investigate how <i>in vivo</i> and <i>in vitro</i> hyperglycemic (HGly) environments can influence the levels of TGF-β₁, decorin, and biglycan during bone repair, with additional consideration for how long-term glucose exposure and cell aging can also influence this process.</p><p><strong>Results: </strong>Following bone healing within a T2DM <i>in vivo</i> model, histological and immunolabeling analyses of bone tissue sections confirmed delayed healing, which was associated with significantly elevated TGF-β₁ levels within the bone matrices of young diabetic rats, compared with their normoglycemic (Norm) and aged counterparts. Studies continued to assess <i>in vitro</i> the effects of normal (5.5 mM) and high (25 mM) glucose exposure on the osteogenic differentiation of compact bone-derived mesenchymal stromal cells (CB-MSCs) at population doubling (PD)15, characterized to contain populations of lineage-committed osteoblasts, and at PD150, where transit-amplifying cells predominate. Short-term glucose exposure increased TGF-β₁ and decorin secretion by committed osteoblasts but had a lesser effect on transit-amplifying cells. In contrast, the long-term exposure of CB-MSCs to high glucose was associated with decreased TGF-β₁ and increased decorin secretion. Similar assessments on macrophage populations indicated high glucose inhibited TGF-β₁ secretion, preventing M2 formation.</p><p><strong>Discussion: </strong>Collectively, these findings highlight how hyperglycemia associated with T2DM can perturb TGF-β₁ and decorin secretion by MSCs and macrophages, thereby potentially influencing TGF-β₁ bioavailability and signaling during bone repair.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1200122"},"PeriodicalIF":1.5,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49315516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}