Frontiers in dental medicine最新文献

{"title":"Effects of Advanced Platelet Rich Fibrin (A-PRF+), Enamel Matrix Derivative (EMD) and Open Flap Debridement on clinical and wound healing parameters in molar furcation sites: A case series from a RCT study.","authors":"L Pitzurra, D Vasdravellis, N A M Rosema, S Bizzarro, B G Loos","doi":"10.3389/fdmed.2023.1223217","DOIUrl":"10.3389/fdmed.2023.1223217","url":null,"abstract":"<p><strong>Aim: </strong>To study the effects of advanced platelet-rich fibrin (A-PRF+) and enamel matrix derivative (EMD) compared to open flap debridement (OFD) alone in molar furcation sites grade II on clinical and wound healing parameters.</p><p><strong>Materials and methods: </strong>A randomized controlled trial was designed. Eligible patients were randomly allocated to one of three treatment groups: A-PRF+, EMD or OFD. The patients and clinical examiners were blinded for the treatment received. A minimally invasive microsurgical approach was performed for the three modalities. Clinical measurements were scored at baseline and 6 months post-operatively. The clinical healing of each furcation was scored via the Early Wound Healing Index on day 3, 1 week, 2 weeks and 6 weeks.</p><p><strong>Results: </strong>17 patients (A-PRF+ <i>n</i> = 6, EMD <i>n</i> = 5, OFD <i>n</i> = 6) completed the 6 months of follow-up. The further completion of the trial had to be cancelled due to the COVID-19 pandemic. In three patients in the A-PRF+ group, the grade II of the treated furcation regressed to grade I; the corresponding number in the EMD and OFD groups was zero and one respectively. Further, 3, 1 and 4 patients in the PRF, EMD and OFD groups respectively, showed a gain of bone level ≥1 mm. The defects in the A-PRF+ group showed delayed early healing compared to the EMD and OFD groups.</p><p><strong>Conclusion: </strong>The case series (RCT design) suggests a slight advantage for A-PRF+ over EMD and OFD, regarding the regressing of a furcation II to grade I; however this treatment showed delayed early wound healing compared to EMD or OFD.</p><p><strong>Clinical trial registration: </strong>https://www.isrctn.com/, identifier ISRCTN13520922.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1223217"},"PeriodicalIF":1.5,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44405041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Bone and metabolic activities.","authors":"T E Van Dyke, N E Hatch, K Joshipura, N E Lane, M Murshed, F Nociti, B Olsen, M J Somerman","doi":"10.3389/fdmed.2023.1244033","DOIUrl":"10.3389/fdmed.2023.1244033","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1244033"},"PeriodicalIF":1.5,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44227757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An engineered biomaterial to harness the differentiation potential of endogenous human gingival mesenchymal stem cells (hGMSCs).","authors":"Mohammad Mahdi Hasani-Sadrabadi, Weihao Yuan, Sevda Sevari, Bo Yu, Sahar Ansari, Alireza Moshaverinia","doi":"10.3389/fdmed.2023.1235096","DOIUrl":"10.3389/fdmed.2023.1235096","url":null,"abstract":"<p><p>Here, we developed a stromal cell-derived factor-1a (SDF-1α) delivery biomaterial as an artificial polymeric-based niche with the ability to recruit local endogenous human gingival mesenchymal stem cells (hGMSCs) for craniofacial bone regeneration applications. Polydopamine-coated poly(ε-caprolactone) (PCL)-gelatin electrospun membranes were loaded with stromal cell-derived factor-1α (SDF-1α) via physical adsorption. Subsequently, the release profile of SDF-1α and the chemotactic capacity on human bone marrow mesenchymal stem cells (hBMMSCs) and hGMSCs were evaluated. The osteogenic differentiation capacity of the recruited MSCs was also assessed <i>in vitro</i>. Our results confirmed the sustainable release of SDF-1α from the developed biomaterial promoting the migration and homing of human bone marrow mesenchymal stem cells (hBMMSCs) and hGMSCs. Moreover, the results of the osteogenic differentiation assay showed that SDF-1α delivery significantly enhanced osteogenic differentiation of hBMMSCs and hGMSCs and up-regulated the gene expression of osteogenic markers compared to the control group. In conclusion, the current study successfully developed a novel and effective treatment modality for craniofacial bone regeneration by recruiting the autogenous progenitor cells including hGMSCs. The developed niches can potentially lead to the development of a novel platform for targeted manipulation of <i>in vivo</i> microenvironment to achieve efficient and safe craniofacial cell reprogramming, which also will pave the road to determine the capacity of local hGMSCs' contribution to <i>in situ</i> bone regeneration.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"1 1","pages":"1235096"},"PeriodicalIF":1.5,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41635453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia exerts disruptive effects on the secretion of TGF-β₁ and its matrix ligands, decorin and biglycan, by mesenchymal sub-populations and macrophages during bone repair.","authors":"Norhayati Yusop, Ryan Moseley, Rachel J Waddington","doi":"10.3389/fdmed.2023.1200122","DOIUrl":"10.3389/fdmed.2023.1200122","url":null,"abstract":"<p><strong>Introduction: </strong>Bone has a high capacity for repair, but for patients with uncontrolled type 2 diabetes mellitus (T2DM), the associated hyperglycemia can significantly delay osteogenic processes. These patients respond poorly to fracture repair and bone grafts, leading to lengthy care plans due to arising complications. Mesenchymal stromal cells (MSCs) and M2 macrophages are both major sources of transforming growth factor-β₁ (TGF-β₁), a recognized mediator for osteogenesis and whose bioavailability and activities are further regulated by matrix small leucine-rich proteoglycans (SLRPs), decorin and biglycan. The aim of this study was to investigate how <i>in vivo</i> and <i>in vitro</i> hyperglycemic (HGly) environments can influence the levels of TGF-β₁, decorin, and biglycan during bone repair, with additional consideration for how long-term glucose exposure and cell aging can also influence this process.</p><p><strong>Results: </strong>Following bone healing within a T2DM <i>in vivo</i> model, histological and immunolabeling analyses of bone tissue sections confirmed delayed healing, which was associated with significantly elevated TGF-β₁ levels within the bone matrices of young diabetic rats, compared with their normoglycemic (Norm) and aged counterparts. Studies continued to assess <i>in vitro</i> the effects of normal (5.5 mM) and high (25 mM) glucose exposure on the osteogenic differentiation of compact bone-derived mesenchymal stromal cells (CB-MSCs) at population doubling (PD)15, characterized to contain populations of lineage-committed osteoblasts, and at PD150, where transit-amplifying cells predominate. Short-term glucose exposure increased TGF-β₁ and decorin secretion by committed osteoblasts but had a lesser effect on transit-amplifying cells. In contrast, the long-term exposure of CB-MSCs to high glucose was associated with decreased TGF-β₁ and increased decorin secretion. Similar assessments on macrophage populations indicated high glucose inhibited TGF-β₁ secretion, preventing M2 formation.</p><p><strong>Discussion: </strong>Collectively, these findings highlight how hyperglycemia associated with T2DM can perturb TGF-β₁ and decorin secretion by MSCs and macrophages, thereby potentially influencing TGF-β₁ bioavailability and signaling during bone repair.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1200122"},"PeriodicalIF":1.5,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49315516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Species diversity in evolution and replacement of teeth informing clinical therapies.","authors":"Christopher A McCulloch","doi":"10.3389/fdmed.2023.1225665","DOIUrl":"10.3389/fdmed.2023.1225665","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1225665"},"PeriodicalIF":1.5,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48339102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: Delayed autologous tooth transplantation based on objective bone healing of the extraction socket (4-year follow-up).","authors":"Yusuke Takahashi, Shotaro Abe, Motoki Okamoto, Tomomi Tsujimoto, Shumei Murakami, Mikako Hayashi","doi":"10.3389/fdmed.2023.1061362","DOIUrl":"10.3389/fdmed.2023.1061362","url":null,"abstract":"<p><p>Autologous tooth transplantation is one of the best methods to replace a missing tooth when there is a suitable donor tooth. Tooth transplantation is mainly performed immediately after extraction because this is completed in a single surgery and the donor tooth is transferred to fresh recipient site facilitated by the remaining periodontal ligament. However, when transplantation is planned for a severe recipient site with a large bone defect surrounding the affected tooth, delayed transplantation is performed because of the mismatched size of the donor tooth. When bone formation at the recipient site is gradually observed during wound healing, transplantation can be performed. However, the estimated time for delayed transplantation has not been clearly determined because of the varied wound healing at the recipient site. This case report demonstrates successful tooth transplantation 4 months after extraction by monitoring bone healing of the recipient site by computed tomography (CT). A male patient complained about occlusal pain in his mandibular molar. He had received the latest restoration after root canal treatment 10 years previously. Seven years later, he experienced slight spontaneous pain and consulted a private dental clinic. Radiographic examination revealed vertical root fracture and the dentist recommended tooth extraction, but he did not receive this suggestion. Several years later, he visited our hospital, and the bone resorption became much larger, and the surrounding bone was completely lost. Thus, it was decided that autologous tooth transplantation should occur several months later because of the poor fit of the donor tooth using wisdom tooth. Sequential CT value was monitored during bone formation at the recipient site by multi-detector computed tomography. Four months later, the CT value of the recipient site had gradually increased and tooth transplantation was performed. Fit of the donor tooth to the recipient site was still poor at the surgery, but it became better and tooth mobility decreased gradually. After performing root canal treatment, final full covered restoration was equipped. Review at 4 years after transplantation revealed the tooth showed no symptoms with no apical radiolucency. This case report suggests that delayed tooth transplantation can be performed after monitoring bone formation at the recipient site by x-ray or CT images.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1061362"},"PeriodicalIF":1.5,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46575940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Frontiers in dental medicine: highlights in systems integration 2021/22.","authors":"Emily Chu, Francisco H Nociti","doi":"10.3389/fdmed.2023.1208248","DOIUrl":"10.3389/fdmed.2023.1208248","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1208248"},"PeriodicalIF":1.5,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44522071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative biomaterials for the treatment of periodontal disease.","authors":"Yi Zhu, Chen Tao, Cynthia Goh, Annie Shrestha","doi":"10.3389/fdmed.2023.1163562","DOIUrl":"10.3389/fdmed.2023.1163562","url":null,"abstract":"<p><p>Periodontitis is a multifactorial disease that involves the destruction of hard and soft tissues surrounding the tooth. Routine periodontal treatment includes mechanical debridement (surgical and non-surgical) and the systemic administration of antibiotics. In contrast, severe and chronic periodontitis involves aggressive tissue destruction and bone resorption, and the damage is usually irreversible. In these severe cases, bone grafts, the delivery of growth hormones, and guided tissue regeneration can all be used to stimulate periodontal regeneration. However, these approaches do not result in consistent and predictable treatment outcomes. As a result, advanced biomaterials have evolved as an adjunctive approach to improve clinical performance. These novel biomaterials are designed to either prolong the release of antibacterial agents or osteogenic molecules, or to act as immunomodulators to promote healing. The first half of this review briefly summarizes the key immune cells and their underlying cellular pathways implicated in periodontitis. Advanced biomaterials designed to promote periodontal regeneration will be highlighted in the second half. Finally, the limitations of the current experimental design and the challenges of translational science will be discussed.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1163562"},"PeriodicalIF":1.5,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43965728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Frontiers in Dental Medicine: highlights in regenerative dentistry 2021/22.","authors":"Waruna Lakmal Dissanayaka, Paul Sharpe","doi":"10.3389/fdmed.2023.1212894","DOIUrl":"10.3389/fdmed.2023.1212894","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1212894"},"PeriodicalIF":1.5,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49397093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fluoride on <i>in vitro</i> hydroxyapatite demineralisation analysed by ¹⁹F MAS-NMR.","authors":"Bajram Ferizoli, Alexander J Cresswell-Boyes, Paul Anderson, Richard J M Lynch, Robert G Hill","doi":"10.3389/fdmed.2023.1171827","DOIUrl":"10.3389/fdmed.2023.1171827","url":null,"abstract":"<p><strong>Introduction: </strong>Fluoride plays a major role in inhibiting enamel dissolution and promoting fluorapatite formation. Porous hydroxyapatite (HAP) discs can be used as an enamel analogue in artificial demineralisation/remineralisation studies.</p><p><strong>Method: </strong>The aim of the study was to monitor the fluoride-mineral phases formed on HAP surfaces as a function of fluoride concentration ([F^-]) under demineralising conditions, using ¹⁹F magic angle spinning nuclear magnetic resonance (MAS-NMR) spectroscopy, and compare the results with a previous study using an enamel substrate. Porous HAP blocks were immersed in demineralisation solutions (0.1 M acetic acid, pH 4.0) with increasing [F^-] (0-1450 ppm).</p><p><strong>Results: </strong>At below 50 ppm [F^-], ¹⁹F MAS-NMR showed fluoride-substituted apatite formation; above 50 ppm [F^-], calcium fluoride (CaF₂) was formed in increasing proportions. These results mirrored those of previous similar studies with an enamel substrate. Further increases in fluoride caused no further measurable reduction in demineralisation but increased the proportion of CaF₂ formed. The total calcium concentration [Ca] and total phosphorus [P] concentrations in the solution were measured by inductively coupled plasma atomic emission spectroscopy. At high fluoride concentrations, the solution total [P] increased, and the molar Ca:P ratios decreased to values consistent with the formation of CaF₂. However, Ca:P ratios found at low [F^-] were higher than those in the previous enamel study and consistent with the formation of a partially fluoridated apatite.</p><p><strong>Conclusions: </strong>Under demineralising conditions, CaF₂ formed on HAP at an [F^-] of 50 ppm and above, whereas fluoridated apatite formed at an [F^-] below 50 ppm. The results were consistent with those obtained when an enamel substrate was used.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":" ","pages":"1171827"},"PeriodicalIF":1.5,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43951452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}