Frontiers in dental medicine最新文献

{"title":"Effect of glycaemic control on growth factor release and <i>in-vitro</i> biological properties of advanced and injectable platelet-rich fibrin in type 2 diabetes Mellitus.","authors":"Rajesh Kashyap Shanker, Anupama Rao, Ashwini Prabhu, Vijaya Kumar, Sowmiya Narayani, Ranajit Das, Aparna Hegde","doi":"10.3389/fdmed.2026.1895392","DOIUrl":"10.3389/fdmed.2026.1895392","url":null,"abstract":"<p><strong>Background: </strong>Platelet-rich fibrin has shown regenerative potential in periodontal treatments; nonetheless, patients with Type 2 diabetes mellitus (T2DM) may experience altered biological activities. Therefore, this study aimed to evaluate the regenerative potential and growth factor release profiles of advanced platelet-rich fibrin (A PRF) and injectable platelet-rich fibrin (i-PRF) derived from participants with controlled and uncontrolled T2DM.</p><p><strong>Methods: </strong>This study compared <i>n</i> = 87 (29 per group) participants-controlled T2DM, uncontrolled T2DM, and Healthy non-diabetic groups-analysing blood samples for HbA1c, platelet count, and growth factors like Vascular endothelial growth factor (VEGF), Platelet-derived growth factor-BB (PDGF-BB), Insulin-like growth factor-1 (IGF-1), Transforming growth factor-beta 1 (TGF-<i>β</i>1), Transforming growth factor-beta 2 (TGF-<i>β</i>2) via Enzyme-linked immunosorbent assay (ELISA), assessing cytocompatibility with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, and employing statistical methods like Analysis of variance (ANOVA) correlation, and regression.</p><p><strong>Results: </strong>PRF derived from participants with uncontrolled T2DM demonstrated significantly reduced PDGF-BB and TGF-<i>β</i>2 release compared with healthy participants in both A-PRF (<i>P</i> = 0.016), i-PRF (<i>P</i> = 0.033), and A-PRF (<i>P</i> = 0.002) and i-PRF (<i>P</i> = 0.0034. VEGF release was significantly lower in uncontrolled T2DM than in well-controlled T2DM in both A-PRF (<i>P</i> = 0.0096) and i-PRF (<i>P</i> = 0.0023). Compared with the uncontrolled T2DM group, well-controlled T2DM exhibited significantly higher VEGF release in both A-PRF (<i>P</i> = 0.0096) and i-PRF (<i>P</i> = 0.0023), and significantly higher IGF-1 (<i>P</i> < 0.05) and TGF-<i>β</i>2 (<i>P</i> = 0.033) levels in i-PRF. Platelet counts were comparable across the study groups, whereas cell viability was highest in PRF from healthy participants. HbA1c was not independently associated with the evaluated growth factor concentrations in multivariable analyses.</p><p><strong>Conclusions: </strong>A-PRF and i-PRF derived from participants with well-controlled T2DM exhibited biological properties comparable to those of healthy non-diabetic individuals, whereas uncontrolled T2DM was associated with reduced release of key growth factors, indicating compromised healing potential. These findings suggest that achieving good glycaemic control may optimize the biological performance of PRF for periodontal regenerative therapy. However, the present findings are based on <i>in vitro</i> analyses; confirmation through well-designed prospective clinical studies remains essential before these findings can be translated into routine clinical practice.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1895392"},"PeriodicalIF":2.7,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13503254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent effects of electron beam irradiation on dental materials: a systematic review of <i>in vitro</i> evidence.","authors":"Shreya Chandrashekar, Niranjan Harikrishna, Avishikta Banerjee, Chaithra Lakshmi V, Nishmitha N Hegde, Mithra N Hegde","doi":"10.3389/fdmed.2026.1871959","DOIUrl":"10.3389/fdmed.2026.1871959","url":null,"abstract":"<p><strong>Background: </strong>Electron beam irradiation (EBI) is a non-chemical technique proposed for modifying dental materials, but evidence is contradictory. Some studies report gains in mechanical performance, others document discoloration, structural breakdown, or cytotoxicity at higher doses.</p><p><strong>Objectives: </strong>To systematically review effects of EBI on mechanical strength, surface characteristics, chemical stability, and biological responses in dental materials, and identify dose ranges where effects shift from beneficial to harmful.</p><p><strong>Methods: </strong>PubMed, Scopus, and Web of Science were searched for laboratory studies published January 2001-March 2026 using the terms \"electron beam irradiation\" and \"dental materials.\" Two reviewers independently screened records; a third resolved disagreements. Eligible studies were laboratory-based experiments evaluating dental materials under EBI. Data were extracted on post-irradiation mechanical, physicochemical, biological, and appearance outcomes. Risk of bias was assessed using RoBDEMAT and QUIN; certainty was rated using a modified GRADE approach.</p><p><strong>Results: </strong>Of 897 records identified, 18 studies met inclusion criteria. Materials tested included composites, nanocomposites, fiber-reinforced composites, denture base polymers, orthodontic bracket materials and adhesives, alloy-resin bonding systems, monolithic zirconia, acrylic resins, glass ionomer cement, dental irrigants, cast titanium, and zinc oxide eugenol. Doses ranged from 1 to 200 kGy, with acceleration energies of 25 kV-10 MeV (two studies did not report beam energy). At low-to-moderate doses, most polymer-based materials showed improved flexural strength, fracture toughness, Vickers hardness, and wear resistance, whereas higher doses caused polymer degradation, discoloration, loss of structural integrity, and genotoxic/cytotoxic effects. QUIN classified 4 studies (22%) as low risk, 12 (67%) as medium risk, and 2 (11%) as high risk of bias.</p><p><strong>Limitations: </strong>All evidence was <i>in vitro</i>; studies were heterogeneous in materials, doses, and outcomes; irradiation parameters were inconsistently reported; modified GRADE certainty was very low across all outcomes.</p><p><strong>Conclusions: </strong>Current <i>in vitro</i> evidence indicates EBI modifies dental material properties, with mechanical improvements most consistent for dimethacrylate-based polymer systems at low-to-moderate doses (1-100 kGy), while color instability and dose-dependent degradation remain important barriers. Because the evidence base is entirely <i>in vitro</i> and of very low certainty, findings are preliminary; dose-optimization and <i>in vivo</i> studies are needed before clinical recommendations can be made.</p><p><strong>Systematic review registration: </strong>https://doi.org/10.17605/OSF.IO/HAVT8.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1871959"},"PeriodicalIF":2.7,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13503174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148820412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decision-making of Jordanian dentists when restoring root canal-treated molars: a cross-sectional vignette survey.","authors":"Sanaa Aljamani, Rasha A Alamoush, Aseel M Sharaireh, Mohammad Al-Rabab'ah, Shanil Rajesh Patel, Emad Moawad, Fadi Jarad","doi":"10.3389/fdmed.2026.1903315","DOIUrl":"10.3389/fdmed.2026.1903315","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to assess the decision-making of Jordanian dentists when restoring root canal-treated molars (RCTMs) and identify the tooth-related factors influencing restorative treatment decisions.</p><p><strong>Methodology: </strong>A previously validated vignette survey, adapted from a UK study, was distributed electronically to registered dentists in Jordan between August and December 2024. The survey comprised three clinical scenarios: UR7 (one remaining wall), LL7 (three remaining walls), and LL6 (one remaining wall with a crack), representing different degrees of structural tooth compromise. Associations between dentist characteristics and restorative preferences were analysed using Fisher-Freeman-Halton exact tests.</p><p><strong>Results: </strong>A total of 364 responses were analysed. Participants comprised 50.8% general dental practitioners and 49.2% specialists; 63% had ≤10 years of experience. Crowns were the preferred restoration for the more structurally compromised molars (Scenarios 1 and 3), whereas onlays predominated in Scenario 2. Zirconia was the most frequently selected material among respondents choosing indirect restorations. Remaining walls, ferrule, marginal ridge loss, and tooth position were the most influential factors, whereas skeletal/lateral guidance and periodontal status were less frequently considered. No statistically significant differences were observed between dentist groups in restorative choices or material preferences (<i>P</i> > 0.05).</p><p><strong>Conclusion: </strong>Jordanian dentists demonstrated broadly consistent decision-making, with indirect restorations generally preferred across the three clinical scenarios. However, variation in consideration of some tooth-related factors suggests opportunities to better align clinical decision-making with evidence-based recommendations.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1903315"},"PeriodicalIF":2.7,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rebuilding oral health systems in post-conflict Syria: a call for integration, innovation, and sustainability.","authors":"Humam Dawood, Nabil Kochaji, Julian Fisher","doi":"10.3389/fdmed.2026.1913447","DOIUrl":"10.3389/fdmed.2026.1913447","url":null,"abstract":"<p><p>Oral health is a vital yet often overlooked component of health system recovery in conflict and post-conflict settings, despite its importance for population well-being, resilience, and social stability. In Syria, more than a decade of conflict has severely disrupted health infrastructure, displaced oral health professionals, and weakened service delivery, exacerbating the burden of untreated oral diseases amid increasing environmental and climate-related stressors. This Perspective explores how Syria could rebuild its oral health system within planetary boundaries, positioning oral health as an integral element of climate-resilient healthcare systems, human security, and health emergency preparedness and response. Drawing on the WHO Global Oral Health Action Plan (2023-2030), the UN 2030 Agenda for Sustainable Development, and lessons from Syria's crisis response, the paper proposes system-level reforms that integrate sustainability, climate resilience, and preparedness across governance, service delivery, workforce education, and supply chains. Key strategic priorities include embedding oral health within primary care and school health programs, promoting prevention-oriented and community-based approaches, adopting climate-resilient and environmentally responsible infrastructure, and institutionalizing emergency preparedness and transdisciplinary competencies within dental education and professional training. Rebuilding oral health systems in post-conflict Syria through a planetary health and human security lens offers a pathway to strengthen health system resilience, safeguard essential services under crisis conditions, and contribute to broader health, environmental, and socioeconomic recovery objectives, with relevance for other fragile and climate-vulnerable settings.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1913447"},"PeriodicalIF":2.7,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148815086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling of pulpal and periapical diseases: a systematic review.","authors":"Ameesha S Rai, Aaron M Iype, Raksha Bhat, Preethesh Shetty","doi":"10.3389/fdmed.2026.1923261","DOIUrl":"https://doi.org/10.3389/fdmed.2026.1923261","url":null,"abstract":"<p><p>Pulpal and periapical diseases represent a significant burden in endodontic practice, yet their molecular pathogenesis remains incompletely understood. Proteomic profiling offers unprecedented opportunities to identify disease-specific biomarkers, elucidate molecular mechanisms, and develop precision diagnostic tools. This systematic review aimed to synthesize the current evidence on proteomic profiling of pulpal and periapical diseases, focusing on key protein biomarkers, molecular pathways, and their potential clinical applications. A comprehensive literature search was conducted across PubMed, Scopus, and Web of Science from January 2009 to June 2026. Studies investigating proteomic analyses of human-derived specimens from patients with pulpal and periapical diseases were included, and data extraction and quality assessment were performed according to PRISMA guidelines. Eleven studies involving 577 participants met the inclusion criteria and analyzed diverse biological specimens, including dental pulp tissue, dentinal fluid, root canal samples, and periapical tissue biopsies. Mass spectrometry-based approaches, including LC-MS/MS, MALDI-TOF MS, and 2D-DIGE, identified 1,153 human proteins and 720 microbial proteins (cumulative counts across all included studies) across different disease states. Key biomarkers included S100 family proteins (S100-A8 and S100-A9), heat shock protein 27 (HSP27), peroxiredoxins, myeloperoxidase, serpin family member SERPINB1, hornerin, and hemoglobin subunits. Distinct proteomic signatures differentiated disease stages, symptomatic and asymptomatic presentations, and post-treatment disease states, while enriched molecular pathways involved neutrophil degranulation, oxidative stress, inflammation, pyroptosis, and bone remodeling. Overall, proteomic profiling reveals distinct molecular signatures across the spectrum of pulpal and periapical diseases, providing valuable insights into disease mechanisms and identifying promising diagnostic and prognostic biomarkers. Future studies should focus on validation in larger cohorts, standardization of sampling protocols, and translation of these findings into chairside diagnostic applications.</p><p><strong>Systematic review registration: </strong>PROSPERO CRD420261452203.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1923261"},"PeriodicalIF":2.7,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13489885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toothbrush and toothpaste preferences among Southern Saudi children: insights into oral hygiene awareness and practices.","authors":"Zuhair Motlak Alkahtani, Hassan Ahmed Assiri, Haifa N Alsharif, Ghydaa Sultan Al-Hufayyan, Jawharah Ali Y Mari, Rawan Saeed Shafloot, Nadiyah Abdullah Alasmari, Rahaf Alshehri, Sahar Alshamrani, Nada Yahya, Amit Porwal","doi":"10.3389/fdmed.2026.1815634","DOIUrl":"https://doi.org/10.3389/fdmed.2026.1815634","url":null,"abstract":"<p><strong>Background: </strong>Oral health is an integral component of general well-being, and the development of good oral hygiene habits during childhood plays a vital role in preventing dental caries and periodontal diseases. Understanding children's preferences regarding toothbrushes and toothpastes can help design effective preventive strategies and enhance compliance with oral hygiene practices.</p><p><strong>Aim: </strong>To assess the awareness, behavior, and preferences of children regarding the selection and use of toothbrushes and toothpastes.</p><p><strong>Materials and methods: </strong>A cross-sectional study was conducted among 500 children aged 5-12 years who visited the Pediatric Dentistry Clinics of King Khalid University, College of Dentistry, from September 2023 to January 2024. A validated self-administered questionnaire with visual aids was used to record demographic data, brushing frequency, and preferences for toothbrush and toothpaste color, flavour, and design. Data were analyzed using IBM SPSS version 20.0, using descriptive statistics and the chi-square test (<i>p</i> < 0.05).</p><p><strong>Results: </strong>Most participants brushed their teeth twice daily (<i>p</i> < 0.05), with a majority brushing independently. Significant gender differences were found: females preferred pink toothpaste (29.2%)/brushes (38.0%), strawberry flavour (55.2%), and design 4 (44.0%), while males preferred blue toothpaste (38.4%)/brushes (45.6%), and mint flavour (32.0%). Males more often chose toothpaste independently (46.4% vs. 36.8%, <i>p</i> = 0.024) and a consistent brand (74.8% vs. 69.2%, <i>p</i> = 0.029).</p><p><strong>Conclusion: </strong>Children demonstrated satisfactory awareness and motivation toward maintaining oral hygiene. However, continued parental supervision and education remain essential to ensure correct brushing techniques and fluoride use. Incorporating sensory preferences, such as colour and flavour, into oral hygiene products may enhance children's compliance and foster lifelong healthy habits.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1815634"},"PeriodicalIF":2.7,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13485750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148801773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation of early implant stability using resonance frequency analysis: a multicenter observational study.","authors":"Shobha J Rodrigues, Vidya Kamalaksh Shenoy, Mithun K Upadhya, Mihir R Kulkarni, A B Tarun Kumar, A A Ponnanna, N S Mamatha","doi":"10.3389/fdmed.2026.1812698","DOIUrl":"https://doi.org/10.3389/fdmed.2026.1812698","url":null,"abstract":"<p><strong>Background: </strong>Implant stability is an important determinant of successful osseointegration and long-term clinical outcomes. Although novel implant designs continue to emerge, independent multicenter clinical evidence evaluating longitudinal implant stability patterns remains limited.</p><p><strong>Aim: </strong>To evaluate longitudinal implant stability patterns of a novel Grade 23 titanium dental implant system using resonance frequency analysis (RFA) across multiple clinical time points.</p><p><strong>Materials and methods: </strong>This multicenter prospective observational study included 230 patients receiving 295 implants across nine academic centers. Implant stability quotient (ISQ) values were recorded at implant placement, second-stage surgery, impression making, and prosthesis trial. Descriptive statistics and non-parametric analyses (Friedman, Wilcoxon signed-rank, and Mann-Whitney <i>U</i> tests) were performed. Spearman correlation analysis was used to evaluate associations between baseline and subsequent ISQ measurements.</p><p><strong>Results: </strong>ISQ values remained generally high throughout the observation period, with mean ISQ values ranging from 73.06 ± 11.66 at implant placement to 75.07 ± 8.23 at the prosthesis trial stage. Mandibular implants demonstrated higher ISQ values than maxillary implants during the early healing stages (<i>p</i> < 0.05), although these differences became less pronounced at later clinical stages. Positive correlations were observed between baseline ISQ values and ISQ measurements recorded at subsequent clinical stages. Thirteen implants failed during osseointegration and two after prosthetic loading, yielding a cumulative survival rate of 94.92%.</p><p><strong>Conclusion: </strong>Within the limitations of this multicenter observational study, the evaluated implant system demonstrated favorable longitudinal ISQ trends during early healing. However, long-term controlled clinical studies incorporating comparative implant systems, radiographic bone level assessment, and extended follow-up are required before definitive clinical recommendations can be established.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1812698"},"PeriodicalIF":2.7,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13485519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in saliva composition during different training phases in professional football players.","authors":"Antoon J M Ligtenberg, Ektor C Petras, Kamran Nazmi, Henny Solleveld, Marja L Laine, Sergio Bizzarro","doi":"10.3389/fdmed.2026.1869353","DOIUrl":"https://doi.org/10.3389/fdmed.2026.1869353","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated changes in salivary biomarkers collected by oral rinsing during different phases of a standard professional football training, and how these changes correlated with perceived training intensity.</p><p><strong>Methods: </strong>This study included 28 professional football players. Saliva was collected by means of oral rinse with water. It was collected at three time-points during a single training session: before training, after warming-up, and after outdoor training. Players were asked to self-report the intensity of the training after each time-point on a scale from 1 to 10. The following biomarkers were measured: protein, carbohydrate and immunoglobulin-A (IgA) concentrations, and enzymatic activity of <i>α</i>-amylase, lactoperoxidase and lysozyme.</p><p><strong>Results: </strong>Protein concentration increased during training (<i>p</i> < 0.001). <i>α</i>-Amylase activity/protein ratio (<i>p</i> < 0.001) increased while the IgA/protein ratio(<i>p</i> < 0.001) and carbohydrates/protein ratio (<i>p</i> = 0.001) significantly decreased. No association was found between the self-perceived intensity of training and changes in the biomarkers.</p><p><strong>Conclusions: </strong>This study shows that saliva composition changes in response to a regular football training session. These changes did not correlate with self-perceived intensity of training.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1869353"},"PeriodicalIF":2.7,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13485790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative antioxidant efficacy of D-Cateslytin combined with modified triple antibiotic paste and calcium hydroxide: an <i>in vitro</i> study.","authors":"Alen Romel, Harish Shetty, Vidhyadhara Shetty, Shravan Kini, Nikhil Harikrishnan, Ashwini Prabhu","doi":"10.3389/fdmed.2026.1921120","DOIUrl":"https://doi.org/10.3389/fdmed.2026.1921120","url":null,"abstract":"<p><strong>Background: </strong>Successful endodontic treatment depends on effective elimination of microorganisms and the creation of a favorable biological environment for periapical healing. Conventional intracanal medicaments such as calcium hydroxide and Modified Triple Antibiotic Paste (MTAP) possess antimicrobial activity; however, their ability to modulate oxidative stress within the root canal environment is limited. D-Cateslytin, a peptide derived from Chromogranin A, has shown promising antimicrobial activity against various oral pathogens and may also contribute to antioxidant effects when used in combination with traditional intracanal medicaments.</p><p><strong>Aim: </strong>To evaluate and compare the antioxidant activity of D-Cateslytin in combination with calcium hydroxide and MTAP.</p><p><strong>Materials and methods: </strong>An <i>in vitro</i> experimental study was conducted using six experimental groups: control, D-Cateslytin, calcium hydroxide, MTAP, D-Cateslytin + calcium hydroxide, and D-Cateslytin + MTAP. The antioxidant activity was assessed by performing 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and nitric oxide (NO) scavenging activity. The absorbance was measured at 517 nm for DPPH and 540 nm for NO.</p><p><strong>Statistical analysis: </strong>Data were analysed using one-way ANOVA followed by Bonferroni <i>post hoc</i> test (<i>p</i> < 0.05).</p><p><strong>Results: </strong>Both D-Cateslytin combination groups demonstrated greater antioxidant activity than calcium hydroxide and MTAP alone. Although the D-Cateslytin + MTAP group exhibited the highest antioxidant activity, it was not significantly different from D-Cateslytin alone or D-Cateslytin + calcium hydroxide.</p><p><strong>Conclusion: </strong>D-Cateslytin exhibited superior antioxidant activity compared to conventional intracanal medicaments. The addition of D-Cateslytin to calcium hydroxide and MTAP may enhance the biological properties of intracanal medicaments and could contribute to improved endodontic treatment outcomes.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1921120"},"PeriodicalIF":2.7,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13485511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary responses to chewing mastic resin and parafilm and inhalation of mastic- and α-pinene-derived volatiles in healthy young adults.","authors":"Zainab Assy, Wiktoria Potocka, Chadidzja Albogatjiew, Asiye Özcan, Floris J Bikker, Marja L Laine","doi":"10.3389/fdmed.2026.1888616","DOIUrl":"https://doi.org/10.3389/fdmed.2026.1888616","url":null,"abstract":"<p><p>Salivary secretion is essential for maintaining oral homeostasis, and its modulation by sensory stimuli provides insight into salivary gland function. Mastic resin, obtained from the <i>Pistacia lentiscus</i> tree, is rich in terpenes such as α-pinene, which may influence salivary responses through gustatory, olfactory, or mechanosensory pathways. In addition, α-pinene may modulate salivary secretion through cholinergic pathways and may therefore have potential as a natural sialagogue. We aimed to evaluate how healthy young adults respond to masticatory and olfactory stimulation by assessing the changes in salivary flow rate and pH following exposure to mastic resin, a tasteless chewing control, and volatiles of mastic oil or α-pinene. The salivary flow and pH were measured in healthy individuals (<i>n</i> = 30) before and after chewing mastic resin or parafilm (control), or after inhalation of mastic or α-pinene oil volatiles delivered via nasal inhalers. Both masticatory stimuli increased salivary flow (<i>p</i> < 0.0001). Chewing mastic resin induced a greater increase in salivary flow than chewing parafilm (<i>p</i> < 0.0001). Olfactory exposure to mastic oil and α-pinene also elicited significant increases in salivary flow (<i>p</i> ≤ 0.01). Furthermore, pH increased after chewing mastic resin and parafilm (<i>p</i> < 0.0001) and α-pinene inhalation (<i>p</i> < 0.05), but not after inhalation of mastic volatiles. In conclusion, healthy young adults demonstrated clear salivary responses to both chewing and odour stimuli derived from <i>P. lentiscus.</i> The findings demonstrate that mastic resin and its volatile components can modulate salivary secretion through multiple sensory pathways, and they warrant further investigation of mastic-derived stimuli as potential modulators of salivary secretion, especially in individuals with dry mouth.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"7 ","pages":"1888616"},"PeriodicalIF":2.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13481199/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148801833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}