Saccharibacteria (TM7) in saliva and subgingival microbiome as a predictor for gingivitis in individuals with type2 diabetes evaluated by qPCR.

IF 1.8 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Frontiers in dental medicine Pub Date : 2025-04-22 eCollection Date: 2025-01-01 DOI:10.3389/fdmed.2025.1550936
Boy M Bachtiar, Dicky L Tahapary, Turmidzi Fath, Citra F Theodorea, Natalina Haerani, Yuniarti Soeroso, Selvi Nafisa Shahab, Ardy Wildan, Fergie Marie Joe Grizella Runtu, Fatimah Maria Tadjoedin, Dewi Ayuningtyas, Lisa Amir, Endang W Bachtiar
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引用次数: 0

Abstract

Oral samples are widely used for studying oral microbiome in health and diseases. In this study, saliva and subgingival biofilm (SGB) samples obtained from patients with type2 diabetes (T2DM), without periodontitis (G1 group), with gingivitis (G2 group), and periodontitis (G3 group), were used to compare the abundance of Saccharibacteria (TM7), its host's bacteria (Schaalia odontolytica), periodontopathogen (Represented by Fusobacterium nucleatum), and nitrate-reducing bacteria (represented by Rothia mucilaginosa). The gingival crevicular fluid were also used to analyze the transcription levels of interleukin-6 (IL-6) and C-reactive protein (CRP). Healthy individuals' oral samples served as a control, and the targeted bacteria and inflammatory indicators were detected and measured using real-time PCR. The results showed that in either sample, the abundance of TM7 and other targeted bacteria showed a similar profile. Notably, within participants with T2DM, the abundance of TM7 was similar in G1 and G2 groups, but significantly decreased in G3 group. With the exception of the SGB of the G3 group, the relationship between TM7 and its bacterial host was strongly positive across all evaluated samples. Furthermore, CRP had higher transcription levels than IL-6 across the entire group. Despite the fact that the G3 group showed an adverse relationship between TM7 and CRP, patients with T2DM generally showed a positive correlation between TM7 and IL-6/CRP, which was verified by a receiver operating curve.

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通过qPCR评估唾液中的糖菌(TM7)和牙龈下微生物组作为2型糖尿病患者牙龈炎的预测因子
口腔样品被广泛用于健康和疾病的口腔微生物组研究。本研究采用无牙周炎(G1组)、牙龈炎(G2组)、牙周炎(G3组)3组2型糖尿病(T2DM)患者的唾液和龈下生物膜(SGB)样本,比较糖菌(TM7)及其宿主细菌(溶牙沙利亚菌)、牙周致病菌(以核梭杆菌为代表)和硝酸盐还原菌(以粘胶罗氏菌为代表)的丰度。同时用龈沟液分析白细胞介素-6 (IL-6)和c反应蛋白(CRP)的转录水平。以健康个体的口腔样本为对照,采用实时荧光定量PCR检测和测量目标细菌和炎症指标。结果表明,在两种样品中,TM7和其他目标细菌的丰度表现出相似的特征。值得注意的是,在T2DM患者中,G1组和G2组的TM7丰度相似,但G3组的TM7丰度明显降低。除G3组的SGB外,TM7与其细菌宿主之间的关系在所有评估样本中均呈强阳性。此外,在整个组中,CRP的转录水平高于IL-6。虽然G3组TM7与CRP呈负相关,但T2DM患者的TM7与IL-6/CRP普遍呈正相关,这一点可以通过受试者工作曲线得到验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.10
自引率
0.00%
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审稿时长
13 weeks
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