Frontiers in dental medicine最新文献

{"title":"Nonsurgical root canal treatment of an Oehlers type IIIa maxillary central incisor with dens invaginatus: a case report.","authors":"Ayumi Inoue, Yuzo Kawanishi, Motoki Okamoto, Mikako Hayashi","doi":"10.3389/fdmed.2024.1458215","DOIUrl":"10.3389/fdmed.2024.1458215","url":null,"abstract":"<p><p>Dens invaginatus has a complex anatomy, making endodontic treatment challenging. We report the case of a 11-year-old girl who presented with radiolucency at the apex of the maxillary central incisor; she was diagnosed with symptomatic apical periodontitis. Cone-beam computed tomography (CBCT) revealed dens invaginatus. The invagination and root canal were observed using a dental operating microscope, and nonsurgical root canal treatment was performed. The invagination was connected to the periodontal tissue, and the tooth was classified as Oehlers type IIIa. Root canal preparations were performed using NiTi files and an ultrasonic device. A calcium hydroxide paste was used as an intracanal dressing. The root canal was filled using a single-cone hydraulic condensation technique with a highly fluid calcium silicate-based sealer. At the 2-year follow-up, no clinical symptoms were observed, and CBCT images revealed no radiographic lesions. Nonsurgical endodontic treatment using CBCT imaging, dental microscope, effective cleaning systems, and a highly fluid sealer facilitated the successful treatment of apical periodontitis attributed to dens invaginatus. The single-cone hydraulic condensation technique using a bio-ceramic sealer is considered effective even in cases with a complex morphology and open apex.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1458215"},"PeriodicalIF":1.5,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiofilm efficacy of plant extracts as root canal irrigants in endodontics: a systematic literature review.","authors":"Jihad Diouchi, Babacar Touré, Sonia Ghoul","doi":"10.3389/fdmed.2024.1479953","DOIUrl":"10.3389/fdmed.2024.1479953","url":null,"abstract":"<p><strong>Background: </strong>To explore the antibiofilm efficacy of plant extracts against <i>in vitro</i> formed single and multispecies endodontic biofilms, in comparison to conventional root canal irrigants.</p><p><strong>Methods: </strong>PubMed, Scopus, Web of Science, and EMBASE were searched up to April 2024. Studies investigating the antibiofilm efficacy, of at least one plant extract and one conventional root canal irrigant, against endodontic biofilms were reviewed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement guidelines. Data were extracted, and studies were critically assessed using the Joanna Briggs Institute checklist.</p><p><strong>Results: </strong>Among 78 articles, eight articles met the criteria and were eventually included in this review. One study showed a high risk of bias, six showed a moderate risk of bias, and one showed a low risk. A total of twelve plant extracts were tested for their antibiofilm efficacy against eight different single-species biofilms and one multispecies biofilm. A combination of microscopy methods and culturing techniques was used for the assessment of their efficacies. Plant extracts exhibited either a biofilm disruption and/or inhibition of biofilm formation. Psidium cattleianum extract and Psidium guajava exhibited enhanced efficacy compared to Chlorhexidine and NaOCl, respectively. Allium sativum demonstrated comparable efficacy to NaOCl. Furthermore, the combination of Cymbopogon martinii essential oil and NaOCl was found to be more effective than either alone when tested on a multispecies biofilm. However, the other plant extracts, such as Mikania Sprengel, Salvadora persica, Camellia sinensis, and Vitis vinifera showed efficacy but were still inferior compared to the control group.</p><p><strong>Conclusions: </strong>Overall, the tested plant extracts demonstrated promising potential for combating <i>in vitro</i> endodontic biofilms. In that context, integrating conventional therapy protocols with plant-inspired treatments may allow effective endodontic biofilm eradication. Hence, future research should focus on optimizing the synergistic combinations of these extracts with NaOCl to maximize the therapeutic outcomes. Heterogeneity amongst the studies prevented a meta-analysis.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1479953"},"PeriodicalIF":1.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Dental treatment under general anesthesia and dental management of a child with congenital ichthyosis.","authors":"Ryoko Hino, Yuta Chiba, Yuriko Maruya, Manami Tadano, Shinji Otake, Seira Hoshikawa, Yoji Sasahara, Kan Saito","doi":"10.3389/fdmed.2024.1481658","DOIUrl":"10.3389/fdmed.2024.1481658","url":null,"abstract":"<p><p>Congenital ichthyosis is a disease in which the stratum corneum on the surface of the skin becomes thick from the time of the fetus and the barrier function of the skin is impaired. Congenital ichthyosis is a genetic disorder that causes ectodermal abnormalities and sometimes affects skin, nails, and tooth enamel. Therefore, some patients require special care in their daily life and during dental treatments. Here, the authors report a case of congenital ichthyosis that developed into severe dental caries at two years and nine months of age. The authors performed whole-exome sequencing in his peripheral blood and found that the patient had compound heterozygous mutations in ALOX12B gene (c.159C>G and c.1579G>A), which is responsible for autosomal recessive congenital ichthyosis-2 (MIM#2421000). Mutation of c.159C>G is a nonsense mutation that has never been reported, therefore novel symptoms might have found. The patients exhibited severe caries by hypoplastic teeth. Here, the authors report the treatment of dental caries in a patient with congenital ichthyosis under general anesthesia and its oral management until mixed dentition.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1481658"},"PeriodicalIF":1.5,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of violet LED with or without a bleaching gel: a 12-month randomized clinical trial.","authors":"Ana Cristina Távora de Albuquerque Lopes, Nair Cristina Margarido Brondino, Juliana Fraga Soares Bombonatti, Rafael Francisco Lia Mondelli","doi":"10.3389/fdmed.2024.1427301","DOIUrl":"10.3389/fdmed.2024.1427301","url":null,"abstract":"<p><strong>Introduction: </strong>The present interventional, controlled, randomized, blind clinical study aimed to evaluate the effectiveness of an in-office bleaching procedure with violet LED associated or not with 37% carbamide peroxide, considering as response variables the degree of change and color stability over 12 months and dental sensitivity over a month.</p><p><strong>Methods: </strong>Forty participants, according to the inclusion and exclusion criteria, were randomly divided into 2 groups (<i>n</i> = 20) according to the bleaching protocol conducted, in two sessions, with a 7-day interval: vLED-violet LED, without gel; vLED/CP-37% carbamide peroxide photocatalyzed with violet LED (control group). In the vLED group, in each session the bleaching was carried out by 2 consecutive irradiation cycles of 25' each (10 × 2' LED + 30\" interval), with 5' interval between cycles. In the vLED/CP group, the gel was applied 5 times in the bleaching session and photocatalyzed 3 times for 2' with 30\" intervals (7'30\" per gel application), totaling 37'30\" per session. Dental sensitivity was assessed using a visual analog scale (VAS) and the effectiveness of bleaching as a function of the degree of change and color stability (<i>Δ</i>E) with a spectrophotometer. The data were tabulated and submitted to statistical tests (<i>p</i> < 0.05).</p><p><strong>Results: </strong>The VAS analysis showed that some individuals from both groups had mild pain (1 ≤ VAS < 4) during the time intervals evaluated, being more prevalent in the vLED/CP group. Regarding the degree of color change, the groups behaved differently over time (<i>p</i> < 0.0001). The <i>Δ</i>E observed for the vLED/CP group was superior in comparison to the vLED group at all evaluated moments.</p><p><strong>Conclusions: </strong>Over 12 months, the vLED/CP group was more effective in relation to the bleaching effect compared to the vLED group. Both groups showed low levels of sensitivity in the studied time intervals.</p><p><strong>Clinical trial registration: </strong>[https://ensaiosclinicos.gov.br/rg/RBR-6rc23h], identifier [U1111-1253-8850].</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1427301"},"PeriodicalIF":1.5,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of oxidative stress in impaired type II diabetic bone repair: scope for antioxidant therapy intervention?","authors":"Pui Li, Kuraym Khalid Kuraym Alenazi, Jordanna Dally, Emma Louise Woods, Rachel Jane Waddington, Ryan Moseley","doi":"10.3389/fdmed.2024.1464009","DOIUrl":"10.3389/fdmed.2024.1464009","url":null,"abstract":"<p><p>Impaired bone healing is a significant complication observed in individuals with type 2 diabetes mellitus (T2DM), leading to prolonged recovery, increased risk of complications, impaired quality of life, and increased risk of patient morbidity. Oxidative stress, resulting from an imbalance between the generation of reactive oxygen species (ROS) and cellular/tissue antioxidant defence mechanisms, has been identified as a critical contributor to the pathogenesis of impaired bone healing in T2DM. Antioxidants have shown promise in mitigating oxidative stress and promoting bone repair, particularly non-enzymic antioxidant entities. This comprehensive narrative review aims to explore the underlying mechanisms and intricate relationship between oxidative stress, impaired bone healing and T2DM, with a specific focus on the current preclinical and clinical evidence advocating the potential of antioxidant therapeutic interventions in improving bone healing outcomes in individuals with T2DM. From the ever-emerging evidence available, it is apparent that exogenously supplemented antioxidants, especially non-enzymic antioxidants, can ameliorate the detrimental effects of oxidative stress, inflammation, and impaired cellular function on bone healing processes during uncontrolled hyperglycaemia; and therefore, hold considerable promise as novel efficacious therapeutic entities. However, despite such conclusions, several important gaps in our knowledge remain to be addressed, including studies involving more sophisticated enzymic antioxidant-based delivery systems, further mechanistic studies into how these antioxidants exert their desirable reparative effects; and more extensive clinical trial studies into the optimisation of antioxidant therapy dosing, frequency, duration and their subsequent biodistribution and bioavailability. By enhancing our understanding of such crucial issues, we can fully exploit the oxidative stress-neutralising properties of these antioxidants to develop effective antioxidant interventions to mitigate impaired bone healing and reduce the associated complications in such T2DM patient populations.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1464009"},"PeriodicalIF":1.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of double adhesive layer application on micro-tensile dentin bond strength of a universal adhesive.","authors":"Afnan O Al-Zain, Asrar Albuqayli, Abdulaziz Albogami, Abdulghani Alkudsi, Meshari Alwabiri, Abdullah T Koshak, Hattan Alsefri, Eliseu A Munchow","doi":"10.3389/fdmed.2024.1484498","DOIUrl":"10.3389/fdmed.2024.1484498","url":null,"abstract":"<p><strong>Introduction: </strong>Achieving optimal dentin bond strength is crucial for the long-term success of adhesive restorations. This study aims to evaluate the impact of double adhesive layer application, with and without light curing between applications, on the micro-tensile dentin bond strength (µ-TBS) of a universal adhesive, in comparison to the conventional single-layer application.</p><p><strong>Methods: </strong>Intact human molars were divided into three groups (<i>n</i> = 15) according to the adhesive application technique using a universal self-etch adhesive (Tetric N-Bond Universal, Ivoclar) as follows: (1) according to the manufacturer's instructions, (2) double-layer application without light curing between layers, and (3) double-layer application with light curing between layers. Samples were immediately tested for µ-TBS, with failure types recorded as adhesive, cohesive, or mixed. Representative samples were observed by scanning electron microscopy. Data were analyzed using multiple-way ANOVA (<i>α</i> = 0.05).</p><p><strong>Results: </strong>The double adhesive layer with and without light curing between layers showed similar μ-TBS to that of the control group (<i>p</i> > 0.05).</p><p><strong>Discussion: </strong>From a clinical perspective, these findings suggest that the accurate application of a single layer of a universal adhesive can be as effective as more complex techniques. Additionally, the use of universal bonding agents may have contributed to the outcomes observed in this study. In conclusion, double adhesive layer application and light curing between adhesive layers did not increase the µ-TBS with the universal adhesive agent explored.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1484498"},"PeriodicalIF":1.5,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automating bone loss measurement on periapical radiographs for predicting the periodontitis stage and grade.","authors":"Nazila Ameli, Monica Prasad Gibson, Ida Kornerup, Manuel Lagravere, Mark Gierl, Hollis Lai","doi":"10.3389/fdmed.2024.1479380","DOIUrl":"10.3389/fdmed.2024.1479380","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to develop and evaluate an automated approach for segmenting bone loss (BL) on periapical (PA) radiographs and predicting the stage and grade of periodontitis.</p><p><strong>Methods: </strong>One thousand PA radiographs obtained from 572 patients were utilized for training while a separate set of 1,582 images from 210 patients were used for testing. BL was segmented using a U-Net model, which was trained with augmented datasets to enhance generalizability. Apex detection was performed using YOLO-v9, focusing on identifying apexes of teeth to measure root length. Root length was calculated as the distance between the coordinates of detected apexes and center of cemento-enamel junction (CEJ), which was segmented utilizing a U-Net algorithm. BL percentage (ratio of BL to the root length) was used to predict the stage and grade of periodontitis. Evaluation metrics including accuracy, precision, recall, F1-score, Intersection over Union (IoU), mean absolute error (MAE), intraclass correlation coefficients (ICC), and root mean square error (RMSE) were used to evaluate the models' performance.</p><p><strong>Results: </strong>The U-Net model achieved high accuracy in segmenting BL with 94.9%, 92.9%, and 95.62% on training, validation, and test datasets, respectively. The YOLO-v9 model exhibited a mean Average Precision (mAP) of 66.7% for apex detection, with a precision of 79.6% and recall of 62.4%. The BL percentage calculated from the segmented images and detected apexes demonstrated excellent agreement with clinical assessments, with ICC exceeding 0.94. Stage and grade prediction for periodontitis showed robust performance specifically for advanced stages (III/IV) and grades (C) with an F1-score of 0.945 and 0.83, respectively.</p><p><strong>Conclusion: </strong>The integration of U-Net and YOLO-v9 models for BL segmentation and apex detection on PA radiographs proved effective in enhancing the accuracy and reliability of periodontitis diagnosis and grading.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1479380"},"PeriodicalIF":1.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-year clinical performance of direct restorations using low-shrinkage Giomer vs. nano-hybrid resin composite.","authors":"Tugba Toz-Akalin, Funda Öztürk-Bozkurt, Mahmut Kusdemir, Alev Özsoy, Emir Yüzbaşıoğlu, Mutlu Özcan","doi":"10.3389/fdmed.2024.1459473","DOIUrl":"10.3389/fdmed.2024.1459473","url":null,"abstract":"<p><strong>Objectives: </strong>The objective of this investigation was to compare the clinical performance of a nano-hybrid resin composite and a low-shrinkage Giomer resin composite.</p><p><strong>Material and methods: </strong>In total, 35 pairs of restorations were performed using either low-shrinkage Giomer (Beautifil II LS, Shofu Inc.) or nano-hybrid (Clearfil Majesty Posterior) resin composite in 35 patients by two operators using the relevant adhesives, i.e., FL-Bond II (Shofu Inc.) and Clearfil SE Bond (Kuraray), with the self-etching technique according to each manufacturer's instructions. Two clinicians assessed the restorations 2 weeks (baseline); 6 months; and 1, 2, and 3 years after the restorative procedures using FDI (World Dental Federation) criteria (Scores 1-5). Data were analyzed using the marginal homogeneity and McNemar tests. The survival rate was calculated using Kaplan-Meier survival analysis and the survival of the two groups was compared with the log-rank test (<i>p</i> = 0.05).</p><p><strong>Results: </strong>The mean observation period was 37.7 ± 6.8 months. All restorations completed their 3-year follow-up. The criteria were mainly rated with high (1 or 2) scores for quality in both groups. Only one restoration in the low-shrinkage Giomer resin composite group was accepted as a failure at the 2-year recall due to retention loss.</p><p><strong>Conclusion: </strong>At the 3-year follow-up, the performance of the restorations using the Giomer and the nano-hybrid resin composite were similar and clinically acceptable.</p><p><strong>Clinical relevance: </strong>The low-shrinkage Giomer resin composite exhibited a similar clinical performance to the nano-hybrid resin composite after 3 years in service with both materials displaying minor surface deteriorations at the 3-year recall.</p><p><strong>Clinical trial registration: </strong>https://clinicaltrials.gov, identifier: NCT02823769.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1459473"},"PeriodicalIF":1.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Access to dental services and use of toothpaste with optimal fluoride content in Peruvian children.","authors":"Natalie Hadad-Arrascue, María Claudia Garcés-Elías","doi":"10.3389/fdmed.2024.1467501","DOIUrl":"10.3389/fdmed.2024.1467501","url":null,"abstract":"<p><strong>Background: </strong>Leading international health organizations advise using toothpaste that contains at least 1,000 parts per million of fluoride, emphasizing that this practice should begin with the eruption of the first primary tooth.</p><p><strong>Aim: </strong>To determine the association between access to dental services and the use of toothpaste with 1,000 ppm fluoride or more in Peruvian children under twelve years of age.</p><p><strong>Materials and methods: </strong>A cross-sectional analysis was conducted using data from the 2021 Demographic and Family Health Survey. The main variable studied was the use of toothpaste containing a fluoride concentration of 1,000 ppm or more. Additionally, the access to dental care, the time elapsed since the last dental visit, and the type of healthcare facility were analyzed. Multilevel regression was applied to examine the relationship between variables and the dependent variable, with Peru's 24 regions serving as the analytical level.</p><p><strong>Results: </strong>According to the bivariate analysis, the natural region, area of residence, place of residence, wealth index, and age were associated with the use of fluoride toothpaste with 1,000 ppm or more. In the multilevel analysis, the access to dental services was not associated with the use of fluoride toothpaste with 1,000 ppm or more (<i>p</i> = 0.454); similarly, the time elapsed since the last dental visit also showed no association (<i>p</i> = 0.676), as did the type of healthcare facility (<i>p</i> = 0.752, <i>p</i> = 0.896, <i>p</i> = 0.983).</p><p><strong>Conclusion: </strong>Accessing dental services, the time elapsed since the last dentist visit, and the location where that care was received were not associated with the use of toothpaste containing 1,000 ppm or more of fluoride among Peruvian children during the year 2021.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1467501"},"PeriodicalIF":1.5,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single nucleotide polymorphism rs854560 in paraoxonase-1 regulates the cytodifferentiation of human periodontal ligament cells.","authors":"Risa Masumoto, Chiharu Fujihara, Masahiro Matsumoto, Jirouta Kitagaki, Shinya Murakami","doi":"10.3389/fdmed.2024.1449482","DOIUrl":"10.3389/fdmed.2024.1449482","url":null,"abstract":"<p><p>Aggressive periodontitis (AgP), classified as Stages III or IV and grade C periodontitis, is characterized by the rapid destruction of periodontal tissue. Genetic factors contribute to the pathogenesis of this disease, and familial aggregation of periodontitis is often observed. However, the mechanisms underlying the onset or progression of AgP have not been elucidated. Previously, we performed exome sequencing and identified AgP risk factors in Japanese AgP-patients. However, the small sample size limited our scope for detecting some of the true AgP genetic risk factors. To overcome this limitation, we searched for AgP-related genes more comprehensively from the whole exome sequencing data of the Japanese AgP-patients by extending the filtering criteria range. We identified seven AgP-associated suggestive genes, including the single nucleotide polymorphism (SNP) rs854560 in paraoxonase-1 (<i>PON-1</i>), which is correlated with AgP. However, the mechanism(s) underlying the induction of AgP pathogenesis by the SNP rs854560 <i>PON-1</i> has not been elucidated. Thus, we further analyzed the functions of the SNP rs854560 <i>PON-1</i> in human periodontal ligament (HPDL) cells through transfection of the wild-type <i>PON-1</i> (WT) or SNP rs854560 <i>PON-1</i> (mut) into HPDL cells. Real-time PCR indicated that mut had higher mRNA expression of osteogenic related-genes and showed a higher tendency of ALP activity and proliferation. The result suggested that WT <i>PON-1</i> contributes to periodontal tissue homeostasis through appropriate proliferation and cytodifferentiation of HPDL cells, while SNP rs854560 <i>PON-1</i> may mediate excessive calcification of periodontal tissue due to hyper proliferation of HPDL cells, thereby increasing the risk of AgP.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1449482"},"PeriodicalIF":1.5,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}