Frontiers in dental medicine最新文献

{"title":"Oral health knowledge, attitudes, and practices of paediatric nurses caring for hospitalized children.","authors":"Ashley Fletcher, Shauna Hachey, Tracy Doyle","doi":"10.3389/fdmed.2024.1426697","DOIUrl":"10.3389/fdmed.2024.1426697","url":null,"abstract":"<p><strong>Objectives: </strong>Nurses are well positioned to provide oral care to hospitalized children. This study explores pediatric hospital nurses' knowledge, attitudes, practices and perceived barriers to providing oral care.</p><p><strong>Methods: </strong>Using a descriptive cross-sectional design, previously validated surveys were adapted based on input from key stakeholders and administered to all nurses and staff providing patient care on inpatient units (<i>N</i> = 239) of a pediatric hospital.</p><p><strong>Results: </strong>The survey response rate was 40% (<i>N</i> = 96), providing a margin of error of 7.59% (95% C.I.). Most participants were unaware that caries is infectious (51%, <i>n</i> = 49) and caries-producing bacteria is transmissible (35%, <i>n</i> = 34). The majority (57%, <i>n</i> = 52) of participants did not recall oral care content within their formal education or oral care continuing education (88%, <i>n</i> = 81), despite high interest (87%, <i>n</i> = 80). Oral care was rated by most as a priority (85%, <i>n</i> = 81), yet the majority (74%, <i>n</i> = 69) believed it is under performed. More nurses with 6 or more years of experience placed a high or very high value on prioritizing oral health (p = 0.005). Furthermore, most nurses do not assess oral health on admission (63%, <i>n</i> = 60), routinely incorporate oral health into the care plan (45%, <i>n</i> = 43), or document oral care (60%, <i>n</i> = 56). Commonly reported barriers include lack of patient cooperation, medical status, and competing needs.</p><p><strong>Conclusions and outcome: </strong>Despite nurses valuing the importance of oral care and their willingness to learn, oral care practices are lacking, and barriers exist. Future investigation is required to further explore the findings related to barriers to care and lack of practice. These results and future findings will be used to guide institutional oral care policy and education.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1426697"},"PeriodicalIF":1.5,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective: Advancing the science regarding temporomandibular disorders.","authors":"Richard Ohrbach, Alexandre F DaSilva, Mildred C Embree, John W Kusiak","doi":"10.3389/fdmed.2024.1374883","DOIUrl":"10.3389/fdmed.2024.1374883","url":null,"abstract":"<p><p>This Special Issue was initiated in response to the call for improved research by the National Academies of Sciences, Engineering, and Medicine (NASEM) (United States) Consensus Study Report on Temporomandibular Disorders (TMDs), a set of putatively localized musculoskeletal conditions. In this Special Issue, the importance of systems biology for TMDs emerges from each of three separate publications. The importance of systems biology to patients is anchored in two domains-laboratory research and clinical observation. The three publications fully speak to the underlying goals in the NASEM recommendations for initiatives: that research on TMDs needs to broaden, that integration between basic and clinical science needs to improve, and that while better evidence is needed, clinicians need to utilize the evidence that already exists. All three of these initiatives, taken together, would lead to better understanding of these complex diseases and to better care of patients with these diseases.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1374883"},"PeriodicalIF":1.5,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11797808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance among Aerobic Gram-Negative Bacilli isolated from patients with oral inflammatory dysbiotic conditions-a retrospective study.","authors":"A Basic, S Blomqvist, G Charalampakis, G Dahlén","doi":"10.3389/fdmed.2024.1293202","DOIUrl":"10.3389/fdmed.2024.1293202","url":null,"abstract":"<p><strong>Introduction: </strong>Aerobic gram-negative bacilli (AGNB) are not part of the resident oral microflora but are occasionally found in high abundance under inflammatory dysbiotic conditions at various oral niches. The aim of the present study was to investigate the identity and antibiotic susceptibility of AGNB isolated from patients in Sweden with mucosal lesions, periodontitis, and peri-implantitis, with special attention to antibiotic resistance and on the presence of phenotypic Extended Spectrum Beta-Lactamase (ESBL) isolates.</p><p><strong>Materials and methods: </strong>Microbiolgical samples were harvested from 211 patients in total, experiencing mucosal lesions (<i>N</i> = 113), periodontitis (<i>N</i> = 62), or peri-implantitis (<i>N</i> = 36). The growth of AGNBs was semiquantified by selective and non-selective culture and the strains were isolated, identified, and tested for antibiotic susceptibility. A total of 251 AGNB strains, occurring in moderate to heavy growth (>100 CFU/ml sample), indicating a dysbiotic microbiota, were identified. The disc diffusion method was used for screening of the antibiotic susceptibility of the isolates. Phenotypic identification of ESBL isolates was based on resistance to ceftazidime and/or cefotaxime.</p><p><strong>Results: </strong>The most commonly detected AGNB isolates in oral inflammatory dysbiotic conditions were fermentative species belonging to <i>Enterobacteriaceae</i> e.g. <i>Citrobacter</i> spp.<i>, Enterobacter</i> spp.<i>, Escherichia coli, Klebsiella</i> spp<i>,</i> and the non-fermentative environmental <i>Burkholderia cepacia, Pseudomonas</i> spp., and <i>Stenotrophomonas maltophilia</i>. No clear trends were seen in frequency of the various species in samples from mucosal lesions, severe periodontitis, and peri-implantitis cases. The 138 <i>Enterobacteriaceae</i> isolates and 113 environmental AGNB isolated showed a high antibiotic resistance in general against antibiotics commonly used in dentistry (Amoxicillin, Amoxicillin + Clavulanic acid, Ampicillin, Clindamycin, Doxycycline, Erythromycin, Oxacillin, PenicillinV, and Tetracycline). The majority of these isolates were susceptible to ciprofloxacin. Ten isolates (4.1%) were phenotypically classified as ESBL positive. The ESBL isolates were predominantly found among isolates of <i>S. maltophilia</i>, while only one ESBL positive isolate was found among <i>Enterobacteriaceae</i>.</p><p><strong>Conclusions: </strong>Phenotypically identified ESBL isolates can occasionally be present among oral AGNB strains isolated in abundance from the dysbiotic microbiota occurring in cases with oral mucosal lesions, severe periodontitis, or peri-implantitis.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":"1293202"},"PeriodicalIF":1.5,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of visual acuity screening in dental education amongst undergraduate dental students: a straightforward method","authors":"Jaya Pindoria, S. Abela, Matthew Maguire, Martyn Sherriff, D. Bister","doi":"10.3389/fdmed.2023.1337909","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1337909","url":null,"abstract":"Visual acuity plays a pivotal role in a dental professional's daily performance and screening the students' field of vision in their early formative years ensures successful undergraduate programmes.To compare near and distance visual acuity and stereopsis in first-year and final-year dental students and investigate students' perception of their vision.This was a cross-sectional study involving 100 KCL first- and final-year dental students that underwent assessment of their vision and completed a self-perception questionnaire. Near visual acuity was assessed using a standardised near vision test chart, distance visual acuity using COMPlog (Clinical Vision Measurement Systems Ltd, London, UK) computerised software and stereopsis using the Frisby stereotest. On the basis of the Mann–Whitney test, no statistical differences were found between the first-year and final-year students’ near and distance visual acuity, nor in stereopsis difference at a significance level of α = 0.05. The null hypothesis was accepted.84% of first-year students and 94% in final-year students attained the highest binocular near visual acuity score of 0.50M. Distance visual acuity scores showed a median ETDRS (Early Treatment Diabetic Retinopathy Study) Letters score 94 in the first-year group and 95 in the final-year group. 8% of students were found to have correctable refractive errors in distance visual acuity. The majority of students across both year groups were able to discern 20 s arc of smallest disparity. Final-year students reported worrying about their eyesight significantly more than the first-year students.No statistically significant differences were found in near and distance visual acuity, and stereopsis, between first-year and final-year dental students. However, 8% of students were identified with undiagnosed, correctable refractive errors. The importance of students' vision in clinical dentistry is highlighted and regular eye examination is recommended.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"88 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139440503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic enhancement of hydrophobic dental adhesives: autonomous strengthening, polymerization kinetics, and hydrolytic resistance.","authors":"Burak Korkmaz, Erhan Demirel, Qiang Ye, Anil Misra, Candan Tamerler, Paulette Spencer","doi":"10.3389/fdmed.2024.1373853","DOIUrl":"10.3389/fdmed.2024.1373853","url":null,"abstract":"<p><p>The leading cause of composite restoration failure is recurrent marginal decay. The margin between the composite and tooth is initially sealed by a low-viscosity adhesive, but chemical, physical, and mechanical stresses work synergistically and simultaneously to degrade the adhesive, destroying the interfacial seal and providing an ideal environment for bacteria to proliferate. Our group has been developing self-strengthening adhesives with improved chemical and mechanical characteristics. This paper reports a self-strengthening adhesive formulation that resists hydrolysis-mediated degradation by providing intrinsic reinforcement of the polymer network through synergistic stimulation of free-radical polymerization, sol-gel reaction, and hydrophobicity. Hydrophobic resin formulation (NE1) was developed using HEMA/BisGMA 28/55w/w and 15 wt% MPS. Control (NC1) contained HEMA/BisGMA 28/55 w/w and 15 wt% MES. The polymerization kinetics, water sorption, leachates, and dynamic mechanical properties of the resin samples were investigated. The NC1 and NE1 samples showed comparable polymerization kinetics, degree of conversion and water sorption. In contrast, NC1 showed significantly higher levels of HEMA and BisGMA leachate, indicating faster degradation in ethanol. At day 3, cumulative HEMA leachate for NC1 was tenfold greater than NE1 (<i>p</i> < <i>0.05</i>). Dynamic mechanical properties were measured at 37 and 70°C in both dry and wet conditions. Under dry conditions, the storage moduli of NC1 and NE1 were comparable and the glass transition temperature (<i>T</i> _g) of NC1 was statistically significant lower (<i>p</i> < 0.001) than NE1. Under wet conditions, the storage modulus of NC1 was lower than NE1 and at 70°C there is a threefold difference in storage modulus. At this temperature and under wet conditions, the storage modulus of NC1 is statistically significantly lower (<i>p</i> < 0.001) than NE1. The results indicated that in the wet environment, NE1 provided lower chain mobility, higher crosslink density, and more hydrogen bonds. The newly formulated methacrylate-based adhesive capitalizes on free-radical polymerization, sol-gel reactions, and hydrophobicity to provide enhanced mechanical properties at elevated temperatures in wet environments and hydrolytic stability under aggressive aging conditions.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"5 ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parents’ satisfaction and children's acceptance of nasal compared to oral midazolam for sedation in two consecutive pediatric dental treatments: a randomized controlled study","authors":"Avia Fux-Noy, Qamar Saadi, A. Shmueli, E. Halperson, Diana Ram, M. Moskovitz","doi":"10.3389/fdmed.2023.1296823","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1296823","url":null,"abstract":"Midazolam, by either the oral or the nasal route, is safe and effective in reducing anxiety and improving behavior in children during dental procedures. Aim: to compare both children's acceptance and parents’ satisfaction with midazolam premedication, when administered as an oral syrup or as a nasal spray and explore whether there were positive or negative changes in acceptance during consecutive dental treatment visits.Randomized controlled study among uncooperative 2–6-year-old children who needed at least two similar dental treatments. On each visit, the acceptability of the medication was assessed as good, fair, or poor. The duration of crying after medication administration was recorded. Additionally, parents were asked to rank their satisfaction with the mode of administration.The study group included 60 children; 30 patients received midazolam orally and 30 nasally. Route of administration did not correlate with medication acceptance in the first (p = 0.11) and second visit (p = 0.61). However, in the oral group, medication acceptance of 73% of children deteriorated on the second visit, compared to 33% in the nasal group (p = 0.01). Parents of children in the oral group expressed less satisfaction with premedication administered on the second visit than did parents of children in the nasal group, p = 0.00. Poor medication acceptance at the first visit (p = 0.014) and oral route of administration (p = 0.014) were found to be predictors of poor medication acceptance at the second visit.Acceptance of premedication is expected to deteriorate after the first treatment visit, especially in the oral route of administration. Dentists should consider nasal spray administration for young pediatric patients who need more than one dental treatment. Parents should be prepared for possible resistance or refusal by children.ClinicalTrials.gov, identifier NCT02679781.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"131 38","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross sectional study","authors":"Rosemeire-Arai Yoshida, T. Lobato, Renata Gorjão, Lucas Santiago França, Lívia Araujo Alves, Maria Teresa Botti Rodrigues Santos","doi":"10.3389/fdmed.2023.1208243","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1208243","url":null,"abstract":"Elevated levels of inflammatory mediators in saliva have been described in individuals with cerebral palsy (CP).The aim of this study was to detect and quantify the pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Prevotella intermedia in the saliva of adolescents with CP.This is a cross-sectional study with adolescents with CP to detect periodontopathogens from saliva samples. Initially, saliva was collected from the CP (n = 34) and control groups (n = 31), followed by the gingival index (GI) for distribution of the groups of individuals with CP and control with gingivitis (bleeding on probing ≥ 10%) and without gingivitis. Bacterial DNA was extracted from saliva samples for detection of periodontopathogens by quantitative PCR (q-PCR). Data were analyzed by Mann–Whitney and Kruskal–Wallis tests, analysis of variance t-test (ANOVA) with Tukey–Kramer post-hoc tests (p < 0.05).The quantification of DNA of periodontopathogens in saliva samples showed that adolescents with CP present a variability (22.93–39.56) in the detection of P. gingivalis and that some subjects with CP and gingivitis (n = 4) present high quantification of P. gingivalis (ranging 39.56–37.65), although no significant difference was found between the groups (p > 0.05). A significant contrast was observed for the pathogen P. intermedia when comparing the difference in the control group (p = 0.0396). No major differences were detected in the quantification of periodontopathogens evaluated between the control group and CP.Adolescents with CP showed variability in the detection of DNA of periodontopathogens, especially a great variation in the detection of P gingivalis in saliva of CP with gingivitis.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"17 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Global excellence in dental medicine: South America","authors":"Taia Maria Berto Rezende, M. Napimoga","doi":"10.3389/fdmed.2023.1333268","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1333268","url":null,"abstract":"","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139223931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors that influence parental satisfaction with SDF treatment in healthy children and those with special health care needs","authors":"Y. O. Crystal, J. H. Jang, M. N. Janal, V. H. Raveis","doi":"10.3389/fdmed.2023.1286162","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1286162","url":null,"abstract":"Purpose Silver diamine fluoride (SDF) is used as a caries management agent for the arrest of dentinal caries lesions. The purpose of this study was to evaluate the satisfaction with SDF treatment provided at a university pediatric dentistry clinic, and to identify factors that may contribute to parental dissatisfaction. Methods We obtained retrospective data of children who received SDF treatment at our clinic from February 1st, 2019 to February 28th, 2021. Parents were contacted by phone to participate in a survey that evaluated their satisfaction with the treatment. Satisfaction was evaluated as a function of medical status, ease of treatment, outcome of SDF treatment, esthetics, and understanding of the treatment's goals and side effects using contingency tables and chi-square statistics. Results From 209 children who received SDF treatment, we were able to contact 91 parents by telephone, and 79 agreed to participate. Special healthcare needs (SHCN) patients were over represented in our sample, comprising 22.3% of all treated and 36.7% of participants. More than 90% were satisfied with the treatment, would do it again and would recommend it to others. Among the 49 children who complained of pain, SDF treatment resolved 82% of these complaints. In the subsample with follow-up in our clinic, approximately half of the treated teeth later received restorative treatment or were extracted, and the other half presented without further treatment. Some children received further treatment elsewhere. Parental dissatisfaction was related to staining of the anterior teeth ( p = 0.04), the need for further treatment ( p = 0.02) and a lesser understanding of side effects ( p = 0.002). Conclusion Most parents were satisfied with SDF therapy as a dental treatment choice due to its easy application and desensitizing effects. Our findings indicate that parental understanding of the interim nature of the treatment and staining of the lesions are important to achieve parental satisfaction.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"14 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134991411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative endodontic procedures in immature permanent teeth with pulp necrosis: the impact of microbiology on the clinical and radiographic outcome","authors":"Rayann Sellami, Wannes Van Holm, Nastaran Meschi, Sarah Van Den Heuvel, Martine Pauwels, Tim Verspecht, Kathleen Vandamme, Wim Teughels, Paul Lambrechts","doi":"10.3389/fdmed.2023.1281337","DOIUrl":"https://doi.org/10.3389/fdmed.2023.1281337","url":null,"abstract":"Aims The current study aimed to determine how the regenerative endodontic procedures (REP) disinfection strategy influences overall bacterial load and REP outcomes. Different bacterial species in the teeth were also examined. Methods A previously reported non-randomized controlled clinical research on REP ± leukocyte and platelet-rich fibrin gathered microbial samples from 14 of 29 patients during REP (LPRF). Four microbiological samples were obtained in two treatment sessions. S1 and S2 were taken before and after the first irrigation with 1.5% NaOCl and saline. Samples S3 and S4 were obtained before and after rinsing with 17% EDTA in the second treatment session. Microbial samples were identified using quantitative polymerase chain reaction with species-specific primers. Results The total bacterial load recovered from patients showed a significant ( p &amp;lt; 0.05) decrease after the first treatment and was maintained throughout the second treatment. Fusobacterium nucleatum , Treponema denticola , and Enterococcus faecalis were the most prevalent species in root canals, detected in all analyzed cases (100%), followed by Prevotella intermedia and Tannerella forsythia , both in six of 14 (42.9%) cases. The presence of these abundant species was significantly reduced after S1. Parvimonas micra was present in four of 14 (28.6%) cases and Actinomyces naeslundii in two of 14 (14.3%) cases. Filifactor alocis , Porphyromonas endodontalis , and Porphyromonas gingivalis were each detected in only one of 14 (7.1%) cases. No statistical correlation could be made between bacterial species and clinical or radiographic outcomes due to the small sample size. In the LPRF group, two cases required retreatment due to early post-treatment flare-up, and two other cases presented radiographically a persistent apical periodontitis 3 years after treatment. In the control group, all analyzed cases were clinically asymptomatic after treatment, and radiographically the final periapical index score at the last recall revealed healthy periapices. Conclusion The REP disinfection protocol of the present study seems to be satisfactorily effective in reducing the total bacterial load, omitting clinical symptoms, and inducing periapical bone healing in immature permanent teeth with pulp necrosis. LPRF seems to prevent these outcomes from being achieved and should consequently not be recommended in REP.","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134992003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}