{"title":"4-META/MMA-TBB树脂修复垂直根骨折的实验室研究。","authors":"Huiying Li, Tong Wang, Jing Fu, Jinghan Guo, Zhimin Fang, Keqing Pan, Haiping Xu","doi":"10.3389/fdmed.2025.1593189","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Reattachment of root fragments with appropriate adhesive materials is expected to be the last conservative treatment for preserving teeth with vertical root fractures (VRFs).</p><p><strong>Objective: </strong>This study evaluated the biocompatibility of 4-META/MMA-TBB resin for root repair, compared with iRoot BP Plus, Fuji IX GIC, and Clearfil SA Luting. Fracture resistance and microleakage of the reattached roots were also tested.</p><p><strong>Methods: </strong>The biocompatibility of set materials was evaluated on L929 fibroblasts. Cell Counting Kit-8 (CCK-8) assay, live/dead cells staining and flow cytometry were used to assess cell biocompatibility. VRFs were created in bovine teeth, which were then reattached with set materials (excluding iRoot BP Plus). For the fracture resistance test, the roots were vertically fractured through the root canals (<i>n</i> = 20). The fracture resistance was compared with sound roots (control group) and fracture patterns were observed under a microscope. Microleakage was also tested on the reattached roots (<i>n</i> = 10). Results were analyzed by one-way ANOVA and the Tukey test. The significance level was set at <i>α</i> = 0.05.</p><p><strong>Results: </strong>Clearfil SA Luting group exhibited the highest cytotoxicity. The other test materials had acceptable cytotoxicity, not exceeding Grade 1 [relative growth ratio (RGR) > 75%] in CCK-8. Flow cytometry showed that the proportion of viable cells exposed to 4-META/MMA-TBB resin displayed no significant difference compared with iRoot BP Plus (<i>P</i> > 0.05). The root fracture resistances reattached using 4-META/MMA-TBB resin and Clearfil SA Luting were higher than that by Fuji IX GIC, but lower than those of the control group (<i>P</i> < 0.05). The difference between the two resin groups was statistically insignificant (<i>P</i> > 0.05). As for the microleakage, 4-META/MMA-TBB resin group had the shortest penetration depth, whereas Fuji IX GIC group showed the longest penetration (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>4-META/MMA-TBB resin had acceptable cell biocompatibility for root repair, similar to iRoot BP Plus. It can provide good fracture resistance and excellent sealing effect for reattaching treatment of VRFs.</p>","PeriodicalId":73077,"journal":{"name":"Frontiers in dental medicine","volume":"6 ","pages":"1593189"},"PeriodicalIF":1.5000,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12245879/pdf/","citationCount":"0","resultStr":"{\"title\":\"Laboratory study on reattachment of vertical root fractures using 4-META/MMA-TBB resin.\",\"authors\":\"Huiying Li, Tong Wang, Jing Fu, Jinghan Guo, Zhimin Fang, Keqing Pan, Haiping Xu\",\"doi\":\"10.3389/fdmed.2025.1593189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Reattachment of root fragments with appropriate adhesive materials is expected to be the last conservative treatment for preserving teeth with vertical root fractures (VRFs).</p><p><strong>Objective: </strong>This study evaluated the biocompatibility of 4-META/MMA-TBB resin for root repair, compared with iRoot BP Plus, Fuji IX GIC, and Clearfil SA Luting. Fracture resistance and microleakage of the reattached roots were also tested.</p><p><strong>Methods: </strong>The biocompatibility of set materials was evaluated on L929 fibroblasts. Cell Counting Kit-8 (CCK-8) assay, live/dead cells staining and flow cytometry were used to assess cell biocompatibility. VRFs were created in bovine teeth, which were then reattached with set materials (excluding iRoot BP Plus). For the fracture resistance test, the roots were vertically fractured through the root canals (<i>n</i> = 20). The fracture resistance was compared with sound roots (control group) and fracture patterns were observed under a microscope. Microleakage was also tested on the reattached roots (<i>n</i> = 10). Results were analyzed by one-way ANOVA and the Tukey test. The significance level was set at <i>α</i> = 0.05.</p><p><strong>Results: </strong>Clearfil SA Luting group exhibited the highest cytotoxicity. The other test materials had acceptable cytotoxicity, not exceeding Grade 1 [relative growth ratio (RGR) > 75%] in CCK-8. Flow cytometry showed that the proportion of viable cells exposed to 4-META/MMA-TBB resin displayed no significant difference compared with iRoot BP Plus (<i>P</i> > 0.05). The root fracture resistances reattached using 4-META/MMA-TBB resin and Clearfil SA Luting were higher than that by Fuji IX GIC, but lower than those of the control group (<i>P</i> < 0.05). The difference between the two resin groups was statistically insignificant (<i>P</i> > 0.05). As for the microleakage, 4-META/MMA-TBB resin group had the shortest penetration depth, whereas Fuji IX GIC group showed the longest penetration (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>4-META/MMA-TBB resin had acceptable cell biocompatibility for root repair, similar to iRoot BP Plus. It can provide good fracture resistance and excellent sealing effect for reattaching treatment of VRFs.</p>\",\"PeriodicalId\":73077,\"journal\":{\"name\":\"Frontiers in dental medicine\",\"volume\":\"6 \",\"pages\":\"1593189\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12245879/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in dental medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3389/fdmed.2025.1593189\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in dental medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/fdmed.2025.1593189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
背景：使用合适的粘接剂材料对根碎片进行再附着是保存垂直根裂（VRFs）牙齿的最后保守治疗。目的：本研究评价4-META/MMA-TBB树脂用于根修复的生物相容性，并与iRoot BP Plus、Fuji IX GIC和Clearfil SA Luting进行比较。并对再接根的抗断裂性能和微渗漏性能进行了测试。方法：在L929成纤维细胞上评价材料的生物相容性。细胞计数试剂盒-8 （CCK-8）法、活/死细胞染色及流式细胞术评估细胞生物相容性。vrf是在牛牙齿中创建的，然后用固定的材料重新附着（不包括iRoot BP Plus）。抗断性试验，根通过根管垂直断裂（n = 20）。与健康根（对照组）进行抗折性比较，并在显微镜下观察骨折形态。对再附着根进行微渗漏试验（n = 10）。结果采用单因素方差分析和Tukey检验进行分析。显著性水平设为α = 0.05。结果：清filsa芦亭组细胞毒性最强。其他测试材料具有可接受的细胞毒性，不超过CCK-8的1级[相对生长比(RGR) > 75%]。流式细胞术显示，与iRoot BP Plus相比，4-META/MMA-TBB树脂处理的活细胞比例无显著差异（P < 0.05）。4-META/MMA-TBB树脂和Clearfil SA Luting的根断裂阻力高于富士IX GIC，但低于对照组（P < 0.05）。对于微渗漏，4-META/MMA-TBB树脂组渗透深度最短，Fuji IX GIC组渗透深度最长(P)。结论：4-META/MMA-TBB树脂具有可接受的根修复细胞生物相容性，与iRoot BP Plus相似。为vrf的再接治疗提供了良好的抗断裂性和良好的密封效果。
Laboratory study on reattachment of vertical root fractures using 4-META/MMA-TBB resin.
Background: Reattachment of root fragments with appropriate adhesive materials is expected to be the last conservative treatment for preserving teeth with vertical root fractures (VRFs).
Objective: This study evaluated the biocompatibility of 4-META/MMA-TBB resin for root repair, compared with iRoot BP Plus, Fuji IX GIC, and Clearfil SA Luting. Fracture resistance and microleakage of the reattached roots were also tested.
Methods: The biocompatibility of set materials was evaluated on L929 fibroblasts. Cell Counting Kit-8 (CCK-8) assay, live/dead cells staining and flow cytometry were used to assess cell biocompatibility. VRFs were created in bovine teeth, which were then reattached with set materials (excluding iRoot BP Plus). For the fracture resistance test, the roots were vertically fractured through the root canals (n = 20). The fracture resistance was compared with sound roots (control group) and fracture patterns were observed under a microscope. Microleakage was also tested on the reattached roots (n = 10). Results were analyzed by one-way ANOVA and the Tukey test. The significance level was set at α = 0.05.
Results: Clearfil SA Luting group exhibited the highest cytotoxicity. The other test materials had acceptable cytotoxicity, not exceeding Grade 1 [relative growth ratio (RGR) > 75%] in CCK-8. Flow cytometry showed that the proportion of viable cells exposed to 4-META/MMA-TBB resin displayed no significant difference compared with iRoot BP Plus (P > 0.05). The root fracture resistances reattached using 4-META/MMA-TBB resin and Clearfil SA Luting were higher than that by Fuji IX GIC, but lower than those of the control group (P < 0.05). The difference between the two resin groups was statistically insignificant (P > 0.05). As for the microleakage, 4-META/MMA-TBB resin group had the shortest penetration depth, whereas Fuji IX GIC group showed the longest penetration (P < 0.05).
Conclusions: 4-META/MMA-TBB resin had acceptable cell biocompatibility for root repair, similar to iRoot BP Plus. It can provide good fracture resistance and excellent sealing effect for reattaching treatment of VRFs.
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