Acta tropica最新文献

{"title":"Corrigendum to:\"Molecular characteristics of Echinococcus multilocularis FABP1 and its regulatory functions on murine macrophages\" [Acta Tropica, volume 255, article number 107247].","authors":"Xiang Li, Peng Liao, Wenjing Zhou, Xinqi Yang, Bin Ye","doi":"10.1016/j.actatropica.2024.107495","DOIUrl":"https://doi.org/10.1016/j.actatropica.2024.107495","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107495"},"PeriodicalIF":2.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hantavirus Pulmonary Syndrome in Northwestern Argentina: Seroprevalence in rodents of Jujuy province and first seropositive record for Euryoryzomys legatus.","authors":"F Cassinelli, W López, R Coelho, J J Martínez, S Kehl, C M Bellomo, V P Martínez, I Ferro","doi":"10.1016/j.actatropica.2024.107500","DOIUrl":"10.1016/j.actatropica.2024.107500","url":null,"abstract":"<p><p>Hantavirus Pulmonary Syndrome (HPS) is a Pan-American emerging infectious disease with a high mortality rate caused by rodent-borne viruses of the genus Orthohantavirus. In Argentina, almost half of the HPS infections occur in the northwestern endemic region. In this study, we evaluated rodent composition, abundance, and antibody prevalence in wild rodents in three subtropical sites: primary forest, secondary forest, and crop fields. From February 2022 to June 2023, we settled a total of 150 traps for three nights every two months reaching a capture-effort of 5,400 trap-nights. We took blood samples from all captured rodents, registered morphological measures, then marked and released. We captured 103 rodents of 5 species (Oligoryzomys chacoensis, Calomys fecundus, Calomys musculinus, Akodon simulator, and Euryoryzomys legatus). The overall antibody prevalence was 10.18 %; with C. fecundus and A. simulator being the species most frequently found to present antibodies, and they were also the dominant species in the captured rodent community. The secondary forest was, by far, the most productive site with 83.5 % of all captures, followed by crop fields (10.68 %) and primary forest (5.82 %). We found that antibody prevalence for C. fecundus was positively correlated to body mass (weight) and size (total length). Another important result of our work is that we report presence of hantavirus antibodies in E. legatus, which represents the first record for this species. These results highlight the importance of continuous monitoring of rodents populations and provide a framework for the planning and implementation of public health prevention campaigns of field workers.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107500"},"PeriodicalIF":2.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular RNAs as a new perspective in the diagnosis and mechanism of Leishmania infections.","authors":"Hamid Alizadeh, Can Muftuoğlu, Zeph Nelson Omondi, Ufuk Mert, Milad Asadi, Ahmet Ozbilgin, Ayse Caner","doi":"10.1016/j.actatropica.2024.107509","DOIUrl":"10.1016/j.actatropica.2024.107509","url":null,"abstract":"<p><p>Leishmaniasis is a neglected infectious disease that affects millions of people worldwide. Visceral leishmaniasis (VL) caused by Leishmania infantum and cutaneous leishmaniasis (CL) caused by L. major/ L. tropica are the main clinical forms of this disease, which are life-threatening if not diagnosed and treated properly. Considering the problems in sampling and laboratory diagnosis of leishmaniasis, new molecular markers such as circular RNAs (circRNAs) are needed. circRNAs, a novel class of RNAs, have been one of the most promising targets for the diagnosis and prognosis of diseases. Although the therapeutic and diagnostic role of circRNAs in many diseases and some parasitic diseases are known, not much research has been done in the field of leishmaniasis. We determined the gene expressions of circRNAs in human leukemia monocytic (THP-1) cells after infection with Leishmania. For this, the human cell line THP-1 was differentiated into macrophages by Phorbol 12-myristate 13-acetate (PMA) treatment. Differentiated THP-1 cells were infected with L. infantum and L. tropica promastigotes. After 24 hours, expression levels of circRNAs were determined by RT-qPCR technique. Also, the microRNAs associated with differentially expressed circRNAs were investigated. Then, the molecular pathways associated with expressed circRNAs were obtained by GO and Reactome. The results showed that five circRNAs were differentially expressed in THP1 macrophages infected with L. infantum and L. tropica. These findings suggest that some circRNAs may be potential biomarkers for diagnosis in Leishmania-infected patients. The enrichment analysis revealed that differentially expressed circRNAs are mainly involved in the regulation of protein stability, RNA catabolic process, and P53/PTK6 signaling mechanism. This is the first study to report an overview of Leishmania-induced circRNAs, which can be potential biomarker candidate for diagnosis especially at species level. Notably, expression of some circRNAs in supernatant of Leishmania infected macrophages suggests that these genes are available in body fluids, therefore, can easily be accessed from the patient without invasive methods especially during treatment monitoring.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107509"},"PeriodicalIF":2.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ToRCH pathogens-induced histopathological changes in placental tissues and associated post obstetric complications.","authors":"Maryam Naseem, Sanaullah Khan, Dalal Sulaiman Alshaya, Tawaf Ali Shah, Sarwat Noreen, Faiz Ur Rehman, Kotab A Attia, Nuzhat Sultana","doi":"10.1016/j.actatropica.2024.107466","DOIUrl":"10.1016/j.actatropica.2024.107466","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Maternal infections caused by the ToRCH complex, comprising Toxoplasma gondii (T.gondii), Rubella Virus (RV), Cytomegalovirus (CMV), and Herpes Simplex Virus (HSV), are significant contributors to Bad Obstetric History (BOH). These infections can vertically transmit through the placental barrier, leading to complications in fetal development. This study investigates the histopathological changes induced by ToRCH pathogens in placental tissues and their association with post-obstetric complications in Pakistani women.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methodology: &lt;/strong&gt;A total of 83 women were enrolled in the study and divided into two groups: the Experimental group and the Control group. The Experimental group consisted of women with BOH and was further subdivided into two categories: Group A and Group B. Group A comprised women who experienced a recent miscarriage at the time of sample collection, while Group B consisted of women who gave birth normally at the time of sample collection. In contrast, the Control group included women with normal delivery and a normal obstetric history. To investigate ToRCH infections, venous blood samples were analyzed for ToRCH antibodies using enzyme-linked immunosorbent assay (ELISA). Additionally, small pieces of placental tissue collected after miscarriage or delivery were subjected to nucleic acid (NA) detection by conventional polymerase chain reaction (PCR) using ToRCH pathogen-specific primers. Histopathological examination of placental tissue was also performed to identify microscopic changes. The data obtained were analyzed using descriptive statistics (percentages) and chi-square tests to determine significant associations.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The study revealed an overall seroprevalence of ToRCH pathogens of 57.83 % in the study population, with 53.01 % detected in the Experimental group and 4.81 % in the Control group. In the Experimental group, IgM and IgG antibodies were detected in 14.45 % and 37.34 % of participants, respectively, with 1.20 % testing positive for both. Notably, the Control group showed no IgM positivity, while IgG was detected in 4.81 %. Nucleic acid (NA) detection revealed ToRCH pathogens in 54.21 % of placental tissues from the Experimental group only. Specifically, antibodies for T.gondii and RV were most prevalent (19.27 % each), while NA detection was highest for RV, CMV, and HSV (14.45 % each). In contrast, the Control group showed minimal antibody detection, with only 2.40 % positivity for T.gondii and CMV each. Statistical analysis revealed significant differences in ToRCH infection (antibodies and NA) between study groups for CMV only (p &gt; 0.05). Discrepancies between ToRCH antibodies and NA positivity were observed in 75.90 % of cases, with 71.08 % occurring in the Experimental group and 4.81 % in the Control group. Histopathological examination revealed chorionic villitis (CV) in 16.86 % of the Experimental group; specifically 10.84 % in Gro","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107466"},"PeriodicalIF":2.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schistosomiasis status and health impact in preschool-aged children in hard-to-reach areas and populations of Homa Bay County, Kenya.","authors":"Phyllis Munyiva Isaiah, Bryan Nyawanda, Collins Okoyo, Joseph Otieno Oloo, Peter Steinmann","doi":"10.1016/j.actatropica.2024.107511","DOIUrl":"10.1016/j.actatropica.2024.107511","url":null,"abstract":"<p><p>Pediatric schistosomiasis in hard-to-reach communities is poorly understood, hampering tailored interventions. This study investigated the prevalence, intensity, health impact, and risk factors for schistosomiasis in preschool-aged children (PSAC) residing in hard-to-reach areas and populations of Homa Bay County, Kenya. A cross-sectional study was conducted among 476 PSAC aged 2-5 years across 6 islands in Lake Victoria, Homa Bay County, Kenya. Kato-Katz and urine filtration techniques diagnosed Schistosoma mansoni and S. haematobium infections respectively. Fecal occult blood (FOB) was tested to assess morbidity, and questionnaires captured sociodemographic and household water, sanitation, and hygiene data. Logistic regression models explored risk factors. The overall prevalence of S. mansoni was 26.0 %, varying considerably between islands; Ringiti had the highest prevalence (62.1 %), while Sukru had the lowest (7.7 %). No S. haematobium infections were diagnosed. Light infections were most common (15.4 %), followed by moderate (8.5 %) and heavy infections (2.2 %). Significantly increased odds for infection were observed in children aged four (aOR = 4.4, 95 % CI: 1.7-11.2) and five years (aOR = 5.6, 95 % CI: 2.2-14.5), in girls (aOR = 2.5, 95 % CI: 1.3-4.8), and in children living in Ringiti (aOR = 6.1, 95 % CI: 1.8-15.0). We noted a strong association between S. mansoni infection and FOB (aOR= 3.3, 95 % CI: 1.6-7.0). We found a high burden of schistosomiasis among PSAC in hard-to-reach communities of Homa Bay County, emphasizing the urgent need to include them in control programs. FOB could be a promising indicator for assessing schistosomiasis-related morbidity in this age group.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107511"},"PeriodicalIF":2.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective humoral immunity induced by virus-like particles expressing Toxoplasma gondii CST1 or MIC8.","authors":"Jie Mao, Gi-Deok Eom, Keon-Woong Yoon, Su In Heo, Hae-Ji Kang, Ki Back Chu, Eun-Kyung Moon, Fu-Shi Quan","doi":"10.1016/j.actatropica.2024.107501","DOIUrl":"10.1016/j.actatropica.2024.107501","url":null,"abstract":"<p><p>Protective efficacy assessment of toxoplasmosis vaccines, at least at the preclinical level, frequently involves lethal dose challenge infection. Nonetheless, their efficacies remain largely unexplored against low infection doses which better reflects how humans become infected in the real world. In this study, we compared the immunity elicited in mice that were heterologously immunized with recombinant baculovirus and virus-like particles expressing either the cyst wall protein (CST1) or microneme protein 8 (MIC8) of Toxoplasma gondii (T. gondii). We also investigated how these vaccines fared against both light and heavy infection intensities of T. gondii ME49. Interestingly, under light infection intensity, vaccines expressing CST1 induced significantly higher mucosal antibody responses than MIC8. Germinal center B (GC B) cell responses were elicited to a greater extent following immunization with either antigen, regardless of the infection dose. Similarly, both antigens suppressed IFN-γ production in the brains upon heavy infection. The overall vaccine-induced protection was also similar for the two vaccine antigens under heavy infection. However, in lightly infected mice, CST1 conferred improved GC B cell induction and further inhibited IFN-γ and cyst burden than those elicited by MIC8, thereby contributing to better protection. These findings indicated that light infection could be used to identify optimal vaccine candidates, thus highlighting the impact of infection intensity in vaccine efficacy evaluations.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107501"},"PeriodicalIF":2.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immature survival and female longevity of Aedes aegypti under natural winter conditions in the temperate region of Argentina.","authors":"Cristian M Di Battista, Raúl E Campos, Sylvia Fischer","doi":"10.1016/j.actatropica.2024.107507","DOIUrl":"10.1016/j.actatropica.2024.107507","url":null,"abstract":"<p><p>The performance of Aedes aegypti was evaluated under natural winter nutritional and thermal conditions in the temperate region of Argentina. Immature stages were reared using leaf litter as a food source. The rearing was structured in three cohorts, the first started in late-fall, the second in early-winter and the last in mid-winter, and in each cohort two treatments were arranged according to solar exposure (sun and shade). The mean monthly temperature during the experiment ranged from 10.1 °C in July to 14.3 °C in the early days of October. Survival in the experiment was low (average 16.2 %), with a maximum (33 %) in mid-winter cohort. Development time from first instar larva to adult emergence varied between 24 and 103 days, was affected by cohort, treatment and their interaction, and showed an inverse relationship with temperature, particularly in the sun treatment. Individuals from the sun treatments of mid-winter and late-fall cohorts had shorter development times. Adult longevity was very low and was only affected by sex, being higher in females (4.5 days) than in males (3.1 days). The results of this work suggest that although immature stages may complete their development and reach the adult stage during the winter in temperate Argentina, females may not survive long enough to reproduce successfully.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107507"},"PeriodicalIF":2.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-individual polymorphisms in the mitochondrial COI gene of tick-killing Ixodiphagus wasps parasitizing Haemaphysalis flava ticks.","authors":"Yurie Taya, Yuto Shiraki, Samuel Kelava, Naoki Fujisawa, Yuma Ohari, Mackenzie L Kwak, Saori Baba, Hideka Numata, Gita Sadaula Pandey, Yuki Ohsugi, Yuki Katada, Shiho Niwa, Shohei Ogata, Keita Matsuno, Nariaki Nonaka, Ryo Nakao","doi":"10.1016/j.actatropica.2024.107510","DOIUrl":"10.1016/j.actatropica.2024.107510","url":null,"abstract":"<p><p>Ixodid ticks are significant vectors of pathogens affecting both humans and animals. Biological control with natural enemies represents a sustainable tool for managing ticks. However, there is a substantial lack of knowledge about the natural enemies of ticks. Wasps of the genus Ixodiphagus (Encyrtidae) are currently the only known tick-specific parasitoids. While these wasps have been sporadically recorded worldwide, their presence in Eastern Asia is poorly documented. In this study, we investigated the occurrence of Ixodiphagus wasps in field-collected ticks reared on rabbits under laboratory conditions. Ticks were collected from the Hokkaido, Hokuriku, Chugoku, Shikoku, and Kyushu regions in Japan. Out of 1,933 Haemaphysalis ticks infested on rabbits, adult wasps emerged from 15 engorged ticks. All the ticks from which wasps emerged were morphologically and molecularly identified as Haemaphysalis flava. Additionally, wasp DNA was detected in unfed H. flava nymphs using a newly designed Ixodiphagus-specific PCR assay. Among nine experimental sites in the Chugoku region, Ixodiphagus wasps were detected at three sites, with parasitism rates ranging from 1.8 % to 8.1 %. Finally, the mitochondrial COI gene sequences of four wasp and two tick samples were characterized using shotgun sequencing, direct sequencing, and in-fusion cloning approaches. Multiple intra-individual polymorphisms were observed in all the tested samples. Further studies are needed to investigate the relationship between Ixodiphagus wasps and Ixodid ticks. An increased understanding of these parasitoid wasps could contribute to future biological control measures against ticks.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107510"},"PeriodicalIF":2.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of periodic input Ornstein-Uhlenbeck temperature-tick-borne disease transmission coupling mechanism under climate change.","authors":"Ning Wang, Shengqiang Liu","doi":"10.1016/j.actatropica.2024.107490","DOIUrl":"10.1016/j.actatropica.2024.107490","url":null,"abstract":"<p><p>Given the rapid increase in climate change, investigating the impact of climate change on the transmission mechanism of tick-borne diseases is imperative. In order to fully capture the influence of the seasonal variation of temperature, environmental disturbances and the co-feeding transmission on the spread of tick-borne diseases, we propose a novel stochastic dynamical model that couples the mean-reverting Ornstein-Uhlenbeck temperature equation with periodic input to the tick-borne disease model. Through theoretical analysis, we derive sufficient conditions for the extinction of tick populations and the eradication of tick-borne diseases, as well as the stochastic persistence conditions of the system. In numerical simulations, we find that the periodic Ornstein-Uhlenbeck temperature equation can effectively fit the actual temperature data in low, medium, and high latitude regions of China. In risk assessment, we find that at the spatial perspective, low-latitude areas have a higher risk of tick-borne diseases, requiring enhanced control measures; from a temporal perspective, compared to the past, the current stage presents a greater risk of tick-borne diseases when preventive measures are not implemented. Additionally, we observe that larger noise of environment for tick populations favors the extinction of tick populations, while smaller temperature fluctuations, noise on infected hosts and ticks, as well as higher temperature regression rate, are more likely to lead to the extinction of tick-borne diseases. These findings provide crucial insights into understanding the impact of climate change on the transmission mechanism of tick-borne diseases.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107490"},"PeriodicalIF":2.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of Henneguya chirodactyli n. sp. (Cnidaria: Myxosporea), a parasite of the Peruvian morwong Chirodactylus variegatus (Valenciennes, 1833) (Centrarchiformes: Latridae).","authors":"K Mayta, G Sotil, J D Chero","doi":"10.1016/j.actatropica.2024.107488","DOIUrl":"10.1016/j.actatropica.2024.107488","url":null,"abstract":"<p><p>A new species of Henneguya Thélohan, 1892 (Myxosporea: Bivalvulida: Myxobolidae) infecting the internal region of the stomach of the Peruvian morwong Chirodactylus variegatus (Valenciennes, 1833) (Centrarchiformes: Latridae), an economically important fish in Peruvian artisanal fishery, is described. Morphologically, Henneguya chirodactyli n. sp. differs from all its congeners due to the combination of myxospore dimensions, polar filament coil count, and an external envelope in the spore body. Phylogenetic analysis based on 18S rRNA gene sequences places this new species as sister to H. lagunensis de Azevedo, Negrelli, de Oliveira, Abdallah, Camara, Matos and Vieira, 2021. Furthermore, this species is located in a clade composed of 12 species of Henneguya and one of Myxobolus Bütschli, 1882, all of which infect marine fish. We emphasize that this is the first study performed with an integrative approach, including morphological (external), scanning electron microscopy (SEM) and molecular descriptions, of a Henneguya species from a Peruvian marine environment.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107488"},"PeriodicalIF":2.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}