Acta tropica最新文献

{"title":"One Health determinants of clinically assessed cutaneous leishmaniasis in Sodo District, Ethiopia: a community-based cross-sectional study.","authors":"Lina Gazu, Zerish Zethu Nkosi, Nigatu Kebede","doi":"10.1016/j.actatropica.2026.108375","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108375","url":null,"abstract":"<p><p>Cutaneous leishmaniasis (CL) is a neglected tropical disease shaped by interacting environmental, wildlife, household, and behavioral factors, yet few community-based studies have evaluated these determinants simultaneously within a One Health framework. We assessed multidomain determinants of clinically assessed CL among 1,356 individuals from six kebeles in Sodo District, Ethiopia. CL was defined as an active lesion or typical scar consistent with current or previous infection. Exposures were grouped into four analytic domains: household/structural, environmental, behavioral, and reservoir/animal-interface; demographic variables were used as adjustment covariates. Domain-specific multivariable logistic regression models were followed by additive domain-level risk scores and an integrated model adjusted for age, sex, education, occupation, and kebele. CL prevalence was 9.1% (123/1,356). In the integrated model (N = 1,266), each additional household/structural risk factor increased the odds of CL (adjusted odds ratio [AOR] = 2.52, 95% CI: 1.65-3.92), environmental risk showed a borderline association (AOR = 1.37, 95% CI: 1.00-1.88), behavioral risk demonstrated the strongest effect (AOR = 4.71, 95% CI: 3.44-6.71), and reservoir/animal-interface risk remained independently associated (AOR = 2.87, 95% CI: 1.35-6.41). Predicted probabilities increased sharply across behavioral risk levels, indicating a dose-response pattern. The integrated model demonstrated excellent apparent discrimination (AUC = 0.92). These findings indicate that clinically assessed CL in this setting reflects cumulative multidomain vulnerability, supporting One Health interventions that address housing, environmental exposures, risky behaviors, and human-reservoir contact.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108375"},"PeriodicalIF":2.6,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149219796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on \"Mathematical modeling and spatial evolutionary analysis of tuberculosis transmission across diverse demographic settings in West Java, Indonesia\".","authors":"Rakesh Arya, Amit Kumar Mohapatra, Sanjeeb Kumar Kar","doi":"10.1016/j.actatropica.2026.108374","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108374","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108374"},"PeriodicalIF":2.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149214930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering spatially heterogeneous predictors of reported malaria cases in the Ashanti Region of Ghana: a triangulated framework integrating correlation analysis, random forest, and multiscale geographically weighted regression.","authors":"Prince Obosu, Pius K Appau, Andrews Ofosu","doi":"10.1016/j.actatropica.2026.108372","DOIUrl":"10.1016/j.actatropica.2026.108372","url":null,"abstract":"<p><strong>Introduction: </strong>Malaria remains a major cause of illness and death in sub-Saharan Africa. In 2023, WHO reported 263 million cases and 597,000 deaths globally. Ghana records a substantial malaria burden, and the Ashanti Region is among the regions contributing a large share of reported cases. Despite successive interventions, reported malaria incidence remains high.</p><p><strong>Method: </strong>This study used a triangulated analytical framework to examine environmental and anthropogenic correlates of district-level reported malaria case counts in 2023 (n = 43 districts). Spearman's rank Correlation, Random Forest, and MGWR models were fitted to raw district-level laboratory-confirmed case counts, and a population-normalized incidence rate was used only for the descriptive spatial map. Spearman's rank correlation characterized global monotonic associations, Random Forest (RF) with partial dependence plots (PDPs) examined nonlinear marginal patterns, and Multiscale Geographically Weighted Regression (MGWR) examined spatially varying local associations, following a variance inflation factor (VIF) screen for multicollinearity.</p><p><strong>Results: </strong>Enhanced Vegetation Index (EVI) was the most consistently ranked correlate across the three analytical methods and was the only RF importance estimate distinguishable from the permutation null at the unadjusted 0.05 level (permutation p = 0.017; not adjusted for 11 comparisons). RF validation showed substantial overfitting and limited out-of-sample generalization (in-sample R² = 0.629; LOOCV R² = 0.056; MAE = 6897 cases; RMSE = 8851 cases; MAPE = 55.8%). Eight predictors had a bandwidth of 3 k-NN, suggesting strong estimated spatial non-stationarity under the MGWR model. With only 43 districts, these narrow bandwidths may also reflect coefficient instability rather than a confirmed spatial scale.</p><p><strong>Conclusion: </strong>Environmental and anthropogenic correlates of district-level reported malaria case counts varied across the Ashanti Region. These ecological associations are exploratory and hypothesis-generating, not evidence of causal transmission mechanisms or intervention effectiveness. Future studies should use reliable population denominators, facility-level coordinates, multiyear surveillance, entomological information, and socioeconomic covariates.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108372"},"PeriodicalIF":2.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and treatment-related predictors of failure of pentavalent antimonial therapy for cutaneous leishmaniasis: a systematic review.","authors":"Homayoon Kazemy, Ahmad Khosravi, Iraj Sharifi, Mehdi Bamorovat, Maryam Hakimi Parizi, Mina Khosravi","doi":"10.1016/j.actatropica.2026.108373","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108373","url":null,"abstract":"<p><strong>Background: </strong>Pentavalent antimonials remain widely used for cutaneous leishmaniasis (CL), but treatment failure prolongs disease and increases patient burden. We synthesized routinely available clinical and treatment-related predictors of antimonial failure.</p><p><strong>Methods: </strong>PubMed/MEDLINE and Europe PMC were searched through 14 August 2026 and Scopus through 30 August 2026, supplemented by citation and targeted website searches. Cohort, case-control, and prediction-model studies of antimony-treated CL were eligible. QUIPS assessed risk of bias. Heterogeneous outcomes, regimens, and overlapping cohorts precluded meta-analysis; results were synthesized following SWiM. The review was not prospectively registered.</p><p><strong>Results: </strong>Of 1,747 records identified, 1,249 were screened and 36 reports included. Prior antimonial treatment and incomplete treatment exposure or adherence most consistently signalled poorer outcomes. Greater lesion burden often predicted failure, but thresholds varied. Lesion duration was context-dependent: prolonged disease and very early non-ulcerated lesions were associated with failure in different settings. Findings for age, sex, comorbidities, geography, and species were inconsistent or context-specific. No model had convincing external validation; a recent model yielded an internally validated C-statistic of 0.608.</p><p><strong>Limitations: </strong>All reports had moderate or high risk of bias. Inconsistent outcome definitions and regimens, suspected cohort overlap, and lack of Embase, Web of Science, and CINAHL searches limit confidence and transportability.</p><p><strong>Conclusions: </strong>The evidence supports documenting treatment history, measuring lesion burden, addressing adherence barriers, and arranging earlier review for higher-risk patients, rather than using an unvalidated score or automatically replacing antimony. Prospective external validation with harmonized failure definitions is needed.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108373"},"PeriodicalIF":2.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Evaluation of an RPA-CRISPR/Cas12a Molecular Assay for Rapid Diagnosis of Theileria annulata Infection in Bovines.","authors":"Aditi Mondal, B C Saravanan, Prachand Pratap Singh, P Madhankumar, M Sankar, Sonalika Mahajan, S Dey, Rajveer Singh Pawaiya","doi":"10.1016/j.actatropica.2026.108371","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108371","url":null,"abstract":"<p><p>Tropical theileriosis, caused by Theileria annulata, continues to be the most significant tick-borne disease affecting cattle, particularly in endemic regions where it leads to significant production losses. Prompt and reliable diagnosis is critically important for timely treatment and disease control; however, conventional diagnostic methods have important limitations. Microscopic examination lacks sensitivity, particularly in carrier animals, while PCR requires specialized laboratory infrastructure. To overcome these limitations, a fluorescence-based RPA-CRISPR/Cas12a assay targeting the multicopy mitochondrial cytb gene was developed for rapid detection of T. annulata. Five RPA primer pairs were designed and evaluated, and primer set-5, generating a 324-bp amplicon, exhibited the best amplification performance. A CRISPR RNA (crRNA) was designed within the selected amplicon to enable specific Cas12a-mediated target recognition. The assay was completed within 50 min and demonstrated no cross-reactivity with DNA from Theileria orientalis, Babesia bigemina, Babesia bovis, Anaplasma marginale, and bovine host DNA, confirming excellent analytical specificity. Sensitivity analysis using recombinant plasmid DNA demonstrated a detection limit of approximately ten copies per reaction. The field evaluation of the assay was done by testing 206 bovine field samples. showed that the developed assay identified 163 positive samples, exceeding the detection rates obtained by PCR (151 positives) and microscopy (116 positives). On comparison with PCR, the assay achieved 100% diagnostic sensitivity, 78.18% specificity, 92.64% positive predictive value, 100% negative predictive value, with an overall agreement of 94.17%. These findings indicate that the RPA-CRISPR/Cas12a platform is a rapid, sensitive, and accurate molecular diagnostic approach with strong potential for field application and point-of-care diagnosis of bovine tropical theileriosis, particularly in resource-constrained settings.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108371"},"PeriodicalIF":2.6,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature seasonality and spatially structured variation in cutaneous leishmaniasis in Algeria: A source-verified national panel, 2000-2024.","authors":"Kaouthar Djehaf, Mohamed Abd El Djalil Djehaf","doi":"10.1016/j.actatropica.2026.108366","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108366","url":null,"abstract":"<p><p>Algeria carries one of the highest cutaneous leishmaniasis (CL) burdens worldwide, yet the climatic structure of its provincial distribution has not been quantified nationally with year-specific covariates. We assembled a balanced panel of all 48 wilayas over 2000-2024 (1200 province-years), linking notified CL counts to annual ERA5-Land bioclimatic indicators, and verified the case series province-by-province against the primary national epidemiological bulletins. Counts were strongly overdispersed and modelled by negative binomial regression with a log-population offset and province-clustered standard errors, then extended with year fixed effects, a within-between decomposition, and BYM2 spatially structured area effects on a shared-border contiguity graph. Temperature seasonality was the dominant bioclimatic correlate (incidence rate ratio 4.28 per standard deviation, 95% CI 2.91-6.29) and the only term robust across non-spatial specifications, concentrated in the between-province component (7.64, 2.71-21.55) with no detectable within-province association (0.92, 0.77-1.10). Spatially structured area effects left the point estimate positive but its interval spanning the null (1.07, 0.93-1.22), confirmed by an independently coded full-posterior cross-check. Annual precipitation was inversely associated under naive standard errors but did not survive province clustering (p=0.063); precipitation seasonality was null under province-clustered inference, but showed a modest inverse association in its within-province component (0.90, 0.82-0.99) and under spatial adjustment (0.91, 0.83-0.98), an exploratory finding. Incidence declined 3.4% per year (0.966, 0.945-0.987), consistent with the separately curated World Health Organization series. The climatic signal in these data is a persistent geographical gradient that spatial adjustment substantially reshapes rather than an identified driver of year-to-year change.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108366"},"PeriodicalIF":2.6,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular monitoring reveals the presence of multiple insecticide resistance markers in mosquito disease vectors in Türkiye.","authors":"Naciye Sena Çağatay, Sofia Balaska, Kostas Mavridis, Mustafa Akiner, John Vontas, Nurper Guz","doi":"10.1016/j.actatropica.2026.108369","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108369","url":null,"abstract":"<p><p>Insecticide resistance in mosquito vectors often involves multiple molecular mechanisms. Here, we analysed resistance-associated mechanisms in Culex pipiens and Aedes albopictus populations from 14 locations across Türkiye using a multi-marker molecular approach. We combined species identification, biotype resolution within the Cx. pipiens complex, and molecular screening of key target-site resistance markers (kdr, CHS1, and ace-1), together with genes associated with metabolic detoxification. Within Cx. pipiens, resistance-associated marker profiles varied among populations with heterogeneous biotype composition, including pipiens, molestus, and hybrid forms. All Cx. pipiens populations exhibited moderate to high frequencies of kdr mutations (L1014F/C; 50-80.8%). The CHS1 I1043M mutation was detected in a subset of populations, reaching frequencies of up to 64 %, whereas ace-1 G119S was detected sporadically at low frequencies (5-10%). In several populations, multiple resistance-associated markers were detected concurrently with varying frequencies, indicating potential complexity in resistance profiles. No target-site resistance mutations were detected in Ae. albopictus at the genotyped loci, and no esterase gene amplification was observed for the assessed markers. Taken together, our findings demonstrate variability in the distribution of resistance markers among mosquito populations across Türkiye. Further phenotypic and ecological investigations are needed to clarify the contribution of insecticide exposure and selection pressures to these patterns. These findings highlight the need for geographically matched and phenotypically validated surveillance frameworks to support evidence based and adaptive vector control strategies.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108369"},"PeriodicalIF":2.6,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrimer-Based Nano Artesunate/β-Glucan as a Promising Antimalarial Candidate: Insights from in Vivo Evaluations.","authors":"Mahla Noorzaei, Faride Khanabadi, Zahra Alimardan, Mostafa Akbariqomi, Taher Elmi","doi":"10.1016/j.actatropica.2026.108365","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108365","url":null,"abstract":"<p><strong>Introduction: </strong>Malaria remains a major global health burden, with increasing concerns regarding resistance to conventional antimalarial therapies, including artemisinin derivatives. This study aimed to develop and evaluate a second-generation dendrimer-based nano artesunate/β-glucan formulation designed to combine antiplasmodial activity with potential immunomodulatory effects against malaria.</p><p><strong>Methods: </strong>A nano artesunate/β-glucan nanocomposite was synthesized using a G2 dendrimer platform and characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), drug loading analysis, solubility assessment, and release kinetics. Antimalarial efficacy was evaluated in a murine Plasmodium berghei model using parasitemia monitoring, lactate dehydrogenase (LDH) assays, and survival analysis. Immunomodulatory effects were assessed through macrophage nitric oxide (NO) production and serum interferon-gamma (IFN-γ) levels. Toxicological evaluation included body weight monitoring, erythrocyte morphology and hemolysis assays, liver enzyme analysis, and histopathological examination of hepatic tissues.</p><p><strong>Findings: </strong>The synthesized nanocomposite showed a mean particle size of 157.5 nm, acceptable size distribution (PDI = 0.284), and spherical morphology. Loading into the dendrimer-based nanocomposite improved the aqueous solubility of artesunate and β-glucan by 83% and 27%, respectively, and enabled sustained drug release over 48 hours. In vivo, the nano artesunate/β-glucan formulation at 100 mg/kg and 80 mg/kg, respectively, achieved complete parasite clearance within 4 days, with no recrudescence during the 21 days of follow-up. This effect was accompanied by increased NO and IFN-gamma levels, suggesting possible immune involvement. Compared with free artesunate and chloroquine, the nanocomposite showed lower hepatotoxicity, minimal hemolysis, preserved erythrocyte morphology, and only minor liver histopathological changes.</p><p><strong>Conclusion: </strong>The dendrimer-based nano artesunate/β-glucan formulation showed potent antiplasmodial activity, improved solubility, sustained release, and a favorable acute safety profile in a murine malaria model. It may represent a promising antimalarial nano formulation, although further mechanistic and long-term safety studies are needed.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108365"},"PeriodicalIF":2.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149178632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and characterization of flea-associated bacteria: genetic diversity and co-occurrence patterns involving Bartonella, Rickettsia, and Wolbachia in Ctenocephalides felis felis from an Atlantic Forest region of Brazil.","authors":"Lizeth Fernanda Banguero Micolta, Lorena Freitas das Neves, Ana Cláudia Calchi, Michelli Inácio Gonçalves Funnicelli, Anna Claudia Baumel Mongruel, Jamille Bispo de Carvalho Teixeira, Katharine Costa Dos Santos, Thammy Vieira Bittar, George Rego Albuquerque, Rosangela Zacarias Machado, Darci Moraes Barros-Battesti, Renata Santiago Alberto Carlos, Marcos Rogério André","doi":"10.1016/j.actatropica.2026.108360","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108360","url":null,"abstract":"<p><p>Ctenocephalides felis felis is a widespread ectoparasite of dogs and cats and has been associated with transmission of several microorganisms of veterinary and public health relevance. This study investigated the molecular occurrence, genetic diversity, and co-occurrence patterns of Bartonella spp., Rickettsia spp., and Wolbachia spp. in fleas collected from dogs and cats in an Atlantic Forest area encompassing the Ilhéus and Itabuna microregions, Bahia State, northeastern Brazil. A total of 1,489 fleas were analyzed, including 1,301 collected from 81 dogs and 188 from 39 cats. PCR targeting the endogenous cox1 gene successfully amplified DNA from 95.77% of fleas collected from dogs and 97.34% of those collected from cats. Quantitative PCR (qPCR) targeting the 16S-23S rRNA internal transcribed spacer (ITS) revealed a higher molecular detection rate of Bartonella spp. in fleas from cats (34.43%) than in those from dogs (8.98%). Conversely, Rickettsia spp. was detected more frequently in fleas collected from dogs (24.8%) than from cats (3.28%). Wolbachia spp. was more frequently detected in female fleas from both dogs (52.8%) and cats (50.3%) than in male fleas (p < 0.05). Triple co-occurrence of the three microorganisms was detected in 0.08% (11/1246) of flea DNA samples positive the cox-1 endogenous gene and subjected to downstream molecular analyses for Bartonella spp., Rickettsia spp., and Wolbachia spp.. Phylogenetic analyses based on multiple molecular markers for Bartonella spp. (gltA, ftsZ, nuoG, and rpoB), Rickettsia spp. (gltA, htrA, ompA, and ompB), and Wolbachia spp. (gatB and wsp) confirmed the detection of Bartonella henselae, Bartonella clarridgeiae, Rickettsia felis, and Wolbachia spp. belonging to supergroups B and F. In fleas collected from dogs, the detection of Wolbachia spp. was associated with a higher predicted probability of Rickettsia spp. detection, whereas in fleas collected from cats it was associated with a lower predicted probability of Bartonella spp. detection. Overall, the findings revealed distinct host-associated molecular detection patterns of Bartonella spp., Rickettsia spp., and Wolbachia spp. in C. felis felis, demonstrating the circulation and genetic diversity of these bacterial agents in flea populations. The detection of zoonotic bacterial agents in fleas highlights their relevance for integrated veterinary and public health surveillance within a One Health framework.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108360"},"PeriodicalIF":2.6,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149134635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid visual detection method for Schistosoma japonicum in Oncomelania hupensis snails using recombinase-aided amplification and lateral flow dipsticks.","authors":"Yuying Ye, Yanhong Liu, Niannian Bi, Chunrong Xiong, Jianfeng Zhang, Song Zhao, Kun Yang","doi":"10.1016/j.actatropica.2026.108361","DOIUrl":"https://doi.org/10.1016/j.actatropica.2026.108361","url":null,"abstract":"<p><strong>Background: </strong>Effective surveillance of infected Oncomelania hupensis snails is pivotal for schistosomiasis elimination, yet traditional methods lack sensitivity and molecular assays require sophisticated equipment.</p><p><strong>Methods: </strong>We developed an RAA-LFD method targeting the SjG28 gene, using biotin-labeled primers and FAM-labeled probes, with isothermal amplification at 39°C for 15 min and visual readout on dipsticks.</p><p><strong>Results: </strong>The assay achieved a detection limit of 1 pg/μL genomic DNA and 10 copies/μL plasmid DNA, with no cross-reactivity against six other parasites. It detected as few as one infected snail among 50 and identified infections from day 7 post-infection.</p><p><strong>Conclusion: </strong>This equipment-free, visual RAA-LFD method offers a practical solution for field-based surveillance, with potential to strengthen schistosomiasis elimination efforts in resource-limited settings.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"108361"},"PeriodicalIF":2.6,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}