First molecular detection of Bartonella spp. in seabirds (Procellariiformes and Sphenisciformes) rescued on the Brazilian coast

IF 2.5 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-09-26 DOI:10.1016/j.actatropica.2025.107854

Daniel Antônio Braga Lee , Dália Monique Ribeiro Machado , Francyelly Monicke Bezerra de Moura , Carolina Pacheco Bertozzi , Vanessa Lanes Ribeiro , Alice Pereira , Jovêncio Mateus Sada , Anna Claudia Baumel Mongruel , Amir Salvador Alabí Córdova , Rosangela Zacarias Machado , Luis Antonio Mathias , Marcos Rogério André

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引用次数: 0

Abstract

The present study aimed to investigate the occurrence and molecular identity of Bartonella spp. in Procellariiformes and Magellanic penguins (Spheniscus magellanicus) rescued along the southern and southeastern coasts of Brazil. A total of 52 blood samples from twelve Procellariiformes species and 170 blood samples and 30 spleen samples from Magellanic penguins were collected along the coasts of Santa Catarina and São Paulo states. Following DNA extraction, all samples were screened by conventional PCR targeting the avian β-actin endogenous gene. Successfully amplified samples were further tested by qPCR targeting the 16S–23S ribosomal DNA internal transcribed spacer (ITS) region for Bartonella detection. Positive samples were subjected to additional conventional PCR assays targeting the gltA, ftsZ, groEL, nuoG, pap31, rpoB, ribC, and 16S–23S (ITS) genic regions for molecular characterization. Overall, 56 samples (22.2 %; 56/252) tested positive in the screening, including five blood samples from Procellariiformes (Thalassarche melanophris, n=3; Thalassarche chlororhynchos, n=2) and 51 biological samples (91.1 %; 51/56) from S. magellanicus (42 spleen, 9 blood). Molecular characterization was successful only for S. magellanicus, with amplification of nuoG (14.2 %; 8/56; four spleens, four blood samples), rpoB (12.5 %; 7/56; four spleens, three blood samples), and ITS (12.5 %; 7/56; four spleens, three blood samples). The obtained sequences were closely related to Bartonella henselae previously detected in humans, cats, mongooses, and ticks associated with Passeriformes. In conclusion, Bartonella genotypes circulate in Procellariiformes, and genotypes closely related to B. henselae are present in Magellanic penguins that arrive in the Brazilian coast.

查看原文本刊更多论文

首次在巴西海岸获救的海鸟（原伞形和蝶形）中检测到巴尔通体。

本研究旨在调查巴西南部和东南部沿海获救的正ellariiformes和麦哲伦企鹅（Spheniscus magellanicus）巴尔通体的发生和分子特征。在圣卡塔琳娜州和圣保罗州海岸共采集了12种原伞虫的52份血液样本和170份麦哲伦企鹅的血液样本和30份脾脏样本。DNA提取后，采用常规PCR方法对禽β-肌动蛋白内源基因进行筛选。成功扩增的样品进一步采用qPCR检测16S-23S核糖体DNA内转录间隔区（ITS），用于巴尔通体检测。阳性样品对gltA、ftsZ、groEL、nuoG、pap31、rpoB、ribC和16S-23S （ITS）基因区进行额外的常规PCR检测，进行分子表征。总体上，56份样本（22.2%,56/252）呈阳性，包括5份原胞虫（黑眼Thalassarche melanophris， n=3; chlororhynchos, n=2）的血液样本和51份麦哲伦链球菌（S. magellanicus， 42份脾脏，9份血液）的生物样本（91.1%,51/56）。只有麦哲伦链球菌分子鉴定成功，扩增了nuoG（14.2%; 8/56； 4个脾脏，4份血样）、rpoB（12.5%; 7/56； 4个脾脏，3份血样）和ITS（12.5%; 7/56； 4个脾脏，3份血样）。所获得的序列与先前在人、猫、猫鼬和蜱中检测到的与passerformes相关的亨塞巴尔通体密切相关。综上所述，巴尔通体基因型在原伞虫中传播，而在到达巴西海岸的麦哲伦企鹅中存在与亨selae密切相关的基因型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.