Spatial distribution of insecticide resistance in Aedes aegypti (Diptera: Culicidae) across Burkina Faso highlights strengthened resistance management strategies

IF 2.5 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-09-20 DOI:10.1016/j.actatropica.2025.107847

Soumanaba Zongo , Hyacinthe K. Toe , Moussa W. Guelbeogo , Antoine Sanou , Alphonse Traore , Madou Tapsoba , Oumarou Tarpaga , Prosper Tiaho , Adama Gansane , N’Falé Sagnon , Brice Bicaba , Moussa Namountougou

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引用次数: 0

Abstract

Responses to recurrent dengue outbreaks rely on insecticide-based tools, effective use of which require understanding of insecticide resistance patterns. A nationwide study across all 13 regions of Burkina Faso was performed to assess countrywide resistance profiles of Aedes aegypti to major insecticide classes for informing decision-makers. WHO tube bioassays were performed under controlled conditions in a centralised location on Ae. aegypti adult females raised from eggs collected in each region. Mosquitoes were exposed to pyrethroids, carbamates, and organophosphates and mortality assessed 24-hours post-exposure. Fifty unexposed mosquitoes per region were genotyped for the F1534C, V1016I, and V410L kdr mutations, which confer resistance to pyrethroids, using melting curve qPCR analysis. Ae. aegypti populations from all 13 regions showed ubiquitous, and often strong resistance to deltamethrin. The F1534C mutation was the most prevalent with overall frequency of 0.89. The V1016I (0.25 - 0.58) and V410L (0.23 - 0.54) mutations were found at moderate and similar frequencies. Populations from seven regions showed resistance to bendiocarb. The resistance to pirimiphos-methyl and especially malathion was generally lower and less than half of the populations classified as resistant in each case. Across the four insecticides all 13 regions showed confirmed or suspected resistance to at least two insecticides and only eight of 13 showed any susceptibility, usually to an organophosphate. Thus, organophosphates, particularly malathion, appear the best overall option for control, and pyrethroid products are likely to show reduced efficacy. However, widespread multiple resistance across these three widely-used insecticide classes presents a challenge to resistance management, and newer alternative classes should be considered and testing built into monitoring programmes.

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布基纳法索埃及伊蚊（双翅目：库蚊科）杀虫剂抗性的空间分布突出表明加强了耐药性管理战略。

应对反复出现的登革热疫情依赖于以杀虫剂为基础的工具，有效使用这些工具需要了解杀虫剂耐药性模式。在布基纳法索所有13个地区开展了一项全国性研究，以评估埃及伊蚊在全国范围内对主要杀虫剂种类的抗性概况，以便向决策者提供信息。世卫组织试管生物测定在控制条件下集中进行。埃及伊蚊成年雌蚊从每个地区收集的卵中饲养。蚊子暴露于拟除虫菊酯、氨基甲酸酯和有机磷，并评估暴露后24小时的死亡率。采用熔点曲线qPCR方法，对每个地区50只未暴露的蚊子进行拟除虫菊酯类杀虫剂抗性基因F1534C、V1016I和V410L突变分型。Ae。所有13个地区的埃及伊蚊种群均对溴氰菊酯表现出普遍存在且往往很强的抗性。F1534C突变最为普遍，总频率为0.89。V1016I（0.25 ~ 0.58）和V410L（0.23 ~ 0.54）突变频率中等且相似。7个地区的种群对恶虫威表现出抗性。对甲基吡虫磷，特别是马拉硫磷的抗性一般较低，在每种情况下被分类为抗性的种群不到一半。在这四种杀虫剂中，所有13个区域都显示出对至少两种杀虫剂的确诊或疑似抗性，13个区域中只有8个显示出敏感性，通常是对一种有机磷农药。因此，有机磷，特别是马拉硫磷，似乎是控制的最佳选择，而拟除虫菊酯类产品的功效可能会降低。然而，这三种广泛使用的杀虫剂类普遍存在多重抗药性，这对抗药性管理构成挑战，应考虑采用更新的替代杀虫剂类，并在监测规划中进行检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.