Acta tropica最新文献

{"title":"Insecticidal activity of phenylpropanoids from Piper sarmentosum and their interactions with glutathione S-transferase from adult mosquitoes","authors":"Arshia Hematpoor ,&nbsp;Norhayu Asib ,&nbsp;Sook Yee Liew ,&nbsp;Vannajan Sanghiran Lee ,&nbsp;Muhammad Afiq Ngadni","doi":"10.1016/j.actatropica.2025.107681","DOIUrl":"10.1016/j.actatropica.2025.107681","url":null,"abstract":"<div><div>This study investigated the insecticidal activity of three phenylpropanoids—asaricin (<strong>1</strong>), isoasarone (<strong>2</strong>), and trans-asarone (<strong>3</strong>)—isolated from the aerial parts of <em>Piper sarmentosum</em> against adult mosquito vectors: <em>Aedes aegypti, Aedes albopictus</em>, and <em>Culex quinquefasciatus</em>. Bioassay-guided fractionation of the plant’s hexane extract led to the isolation of these phenylpropanoids. Compounds <strong>1</strong> and <strong>2</strong> exhibited strong adulticidal effects against <em>Aedes</em> species, with LD₅₀ values ≤ 8.8 µg/mL and LT₅₀ values ≤ 29 min. Meanwhile, <em>Culex quinquefasciatus</em> was less susceptible to these compounds, showing LT₅₀ values ≤ 56 min. In contrast, compound <strong>3</strong> showed moderate toxicity across all species. To explore the potential resistance mechanism, glutathione <em>S</em>-transferase (GST) activity was measured and found to correlate positively with the LD₉₅ values of the compounds. Mosquitoes exposed to all isolated phenylpropanoids at LD₉₅ levels showed a significant increase in GST activity, suggesting its involvement in detoxification. Molecular docking studies further confirmed this interaction, revealing consistent binding of all three compounds to key residues (PRO11, GLU64, SER65, ARG66, and TYR105) within the GST active site. Despite increased GST activity—a known marker of metabolic resistance— compounds <strong>1</strong> and <strong>2</strong> remained highly effective, indicating that their mode of action may overcome or bypass common resistance pathways. These findings highlight the potential of <em>P. sarmentosum</em>-derived phenylpropanoids as promising botanical insecticides and underscore the importance of understanding enzyme-ligand interactions in developing effective mosquito control strategies.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107681"},"PeriodicalIF":2.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of zoonotic malaria using room-temperature stable and ready-to-use colorimetric LAMP reagents","authors":"Yee Ling Lau ,&nbsp;Mohd Lutfi Abdullah ,&nbsp;Meng Yee Lai","doi":"10.1016/j.actatropica.2025.107678","DOIUrl":"10.1016/j.actatropica.2025.107678","url":null,"abstract":"<div><div>Traditional loop-mediated isothermal amplification (LAMP) assays rely on a cold chain to preserve the stability of LAMP reagents during storage and transport. This requirement restricts the application of LAMP-based methods in resource-limited settings. To overcome this challenge, we developed a room-temperature stable, ready-to-use dried format of the LAMP diagnostic tool for detecting zoonotic malaria. In this study, we report the use of LAMP for detecting five zoonotic malaria species including <em>Plasmodium fieldi</em>, as there have been no previous publications on LAMP for <em>fieldi</em> to date. The reaction mixture was spin-dried in a vacuum container and stored with silica gel at room temperature before used. The detection limits were 1 copy/µL for <em>P. cynomolgi, P. coatneyi, P. fieldi</em>, and <em>P. inui</em>, while the detection limit for <em>P. knowlesi</em> was 10 copies/µL. Results can be visually observed through color changes, without the need for specialized equipment. Positive samples were indicated in yellow while negative reactions remained pink. The dehydrated LAMP reagents can be kept at room temperature for 15 days. Combination of the advantages of the raffinose, the dehydrated LAMP assay develop here is an ideal diagnostic tool in resource-limited settings where malaria is endemic.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107678"},"PeriodicalIF":2.1,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence and diversity of Leptospira pathogenic serovars in synanthropic fauna from Guayaquil city in Ecuador","authors":"Solon Alberto Orlando ,&nbsp;Ericka Sanchez ,&nbsp;Naomi Mora-Jaramillo ,&nbsp;Fabiola Jiménez Valenzuela ,&nbsp;Ariana León-Sosa ,&nbsp;Ariana Rivera ,&nbsp;Emma Matamba ,&nbsp;Gabriela Macias ,&nbsp;Gabriela Martinez ,&nbsp;Ana Piña ,&nbsp;Juana Huartanga ,&nbsp;Vicente Solis ,&nbsp;Katherine Paez ,&nbsp;Patricia Sumoy Velez ,&nbsp;Fabricio Arcos ,&nbsp;Angel Sebastián Rodríguez-Pazmiño ,&nbsp;Miguel Angel García-Bereguiain","doi":"10.1016/j.actatropica.2025.107677","DOIUrl":"10.1016/j.actatropica.2025.107677","url":null,"abstract":"<div><div>Leptospirosis is a globally distributed zoonotic disease that affects animal production systems and leads to mortality among its hosts. In Ecuador, this disease is endemic, with records dated from the late 19th century, and adversely impacts numerous animal species. We assessed the seroprevalence and diversity of <em>Leptospira</em> serovars in synanthropic fauna including stray dogs, cats and rats from Guayaquil city in Ecuador using the Microagglutination Test (MAT). Our findings revealed a wide diversity of the 24 serovars studied, affecting all animal species, with Pomoma, Sejroe, Bataviae and Saxkoebing as the most prevalent ones. Moreover, the prevalence of antibodies against <em>Leptospira</em> infection was extremely high with values of 94.7 % (CI 95 %: 76.1–100), 82.1 % (CI 95 %: 66–98.1) and 83.6 % (CI 95 %: 67.2–99.9) in dogs, cats and rats, respectively. Our findings support previous reports showing that leptospirosis is endemic in Ecuador and affects multiple animal species. Moreover, our results would suggest the potential role of synanthropic fauna as a reservoir for <em>Leptospira</em> in urban settings although further research involving <em>Leptospira</em> PCR diagnosis and culture isolation is needed. Therefore, we strongly recommend incorporating a comprehensive approach for surveillance and control of leptospirosis in Ecuador from a One Health perspective.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107677"},"PeriodicalIF":2.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-technology parasite detection: The intersection of tradition and innovation","authors":"ChenYu Wang ,&nbsp;RuoFeng Xu ,&nbsp;YuXiang Zeng ,&nbsp;RuiNing He ,&nbsp;Bo Zhang ,&nbsp;YongKang Huang ,&nbsp;ShuCheng Ma ,&nbsp;YuXuan Zuo ,&nbsp;YuJun Deng ,&nbsp;YanYu Wang ,&nbsp;Tao Xiong","doi":"10.1016/j.actatropica.2025.107652","DOIUrl":"10.1016/j.actatropica.2025.107652","url":null,"abstract":"<div><div>Parasitic diseases remain a serious global public health problem, especially in the Asian, African, and Latin American regions with relatively poor medical conditions. Timely, accurate, and rapid detection is the key to parasitic disease prevention and control, and the use of novel technologies to study worm resistance is of great significance for the clinical treatment of patients. This study provides an overview of the research progress in parasite detection technologies, encompassing both traditional and emerging methods. In the future, integrating multiple technologies and adopting intelligent detection approaches will be instrumental in advancing parasitic disease detection. Future technologies aim to provide a reference for enhancing the detection of parasitic infections and contribute to the protection of human health and public health safety while offering new ideas and methods for diagnosing parasitic diseases.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107652"},"PeriodicalIF":2.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum nitric oxide in chronic Chagas disease: a scoping review","authors":"Patrícia Carla de Oliveira ,&nbsp;Karen Dyminski Parente Ribeiro ,&nbsp;Lilian Pereira Ferrari ,&nbsp;Fernanda Tomiotto-Pellissier ,&nbsp;Iara Jose de Messias Reason","doi":"10.1016/j.actatropica.2025.107673","DOIUrl":"10.1016/j.actatropica.2025.107673","url":null,"abstract":"<div><div>Nitric oxide (NO) plays a complex role in the pathophysiology of chronic Chagas disease (CD), contributing to both host defense and tissue damage. Thus, understanding its clinical implications may support therapeutic strategies. The objective of this scoping review was to map and synthesize the available evidence on the clinical impact of serum NO levels in individuals with chronic CD. A scoping review was conducted following PRISMA-ScR guidelines, in which a systematic search of PubMed, Scopus, and Web of Science was performed. The inclusion criteria were studies that quantified NO or its synthetizing enzymes (NOS) in individuals with chronic CD irrespective of the publication year or the language. Studies that did not assess the levels of NO, its metabolites or NOS, in addition to <em>in vivo</em> and <em>in vitro</em> experimental studies, were excluded. The findings from this scoping review showed that individuals with chronic CD have high serum NO levels, suggesting that excessive production of this marker may lead to oxidative stress and play a role in the pathophysiological process associated with cardiac and digestive complications. Combined therapies using antiparasitic drugs with antioxidants have shown a greater reduction in NO levels compared to conventional drug therapy, indicating a potential to reduce oxidative damage and consequently slow disease progression. Nonetheless, additional studies at various disease stages are necessary to confirm the effectiveness of this approach.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107673"},"PeriodicalIF":2.1,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype patterns and evolutionary rates: uncovering Japanese encephalitis virus spread across Asia's climate regions","authors":"Shijili Mambully ,&nbsp;Varsha Ramesh ,&nbsp;Swati Rani ,&nbsp;Mehnaj Khatoon ,&nbsp;Jayashree A ,&nbsp;Archana V Patil ,&nbsp;Azhahianambi Palavesam ,&nbsp;Pinaki Prasad Sengupta ,&nbsp;Sharanagouda S Patil ,&nbsp;Suresh KP","doi":"10.1016/j.actatropica.2025.107676","DOIUrl":"10.1016/j.actatropica.2025.107676","url":null,"abstract":"<div><div>Japanese Encephalitis Virus (JEV) is a highly endemic zoonotic virus, consistently found in Asia and parts of the Western Pacific, and it’s a major cause of human encephalitis. JEV belongs to a family of antigenically related viruses such as West Nile Virus (WNV), Murray Valley encephalitis virus (MVEV), and Aichi Lake Fever Virus (ALFV) and is transmitted by mosquitoes. Persistent outbreaks of the disease necessitate detailed studies to understand their transmission dynamics and develop effective prevention strategies. This study explores the evolutionary dynamics and spatial transmission of JEV, concentrating on the envelope protein (E) structural gene sequences obtained from across Asia’s diverse climatic regions. Evolutionary modeling of the JEV E gene revealed a higher evolutionary rate in tropical regions compared to temperate regions, with nucleotide substitution rates estimated at 1.12 × 10⁻³ per site per year for tropical regions and 5.284 × 10⁻⁴ for temperate regions. The time to the most recent common ancestor (tMRCA) was traced to 1796 from Korea for temperate regions, and 1865 from Indonesia for tropical regions. Among the five genotypes of JEV, Genotype I (GI) and III (GIII) were established all over Southeast Asia; moreover, GI revealed a higher evolutionary rate, reflecting its adaptability to diverse ecological niches. The phylogeographic analysis highlighted significant contributions to virus diffusion by China, Korea, and Japan in temperate zones and by Vietnam in tropical zones. By analyzing genetic sequences from various regions and time periods, this study delivered valuable intuitions into transmission pathways. The findings highlighted the necessity of ongoing surveillance and evolutionary monitoring to track the spread and emergence of novel variations of JEV, which are crucial not just for managing JEV outbreaks but also for guiding immunization programs and public health initiatives.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107676"},"PeriodicalIF":2.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-155-chitosan polyplex as a novel therapeutic modality against Leishmania major: A feasibility study","authors":"Yasamin Rahmani ,&nbsp;Samira Mohammadi-Yeganeh ,&nbsp;Farshid Yeganeh ,&nbsp;Azam Pourabbasi Ardekan ,&nbsp;Ameneh Koochaki ,&nbsp;Milad Taghizadeh-Anvar ,&nbsp;Mostafa Haji Molla Hoseini","doi":"10.1016/j.actatropica.2025.107674","DOIUrl":"10.1016/j.actatropica.2025.107674","url":null,"abstract":"<div><h3>Background</h3><div>Leishmaniasis is a neglected tropical disease affecting millions of people worldwide. Macrophages play a crucial role in the life cycle of <em>Leishmania</em> and host-parasite interactions. <em>Leishmania</em> parasites have evolved mechanisms to modulate macrophage miRNA, including miR-155, in order to evade immune responses. Modulation of miR-155 levels could potentially restore balanced immune responses while enhancing the host's ability to clear parasites.</div></div><div><h3>Objective</h3><div>This study aims to evaluate the antileishmanial effects of the miR-155 chitosan polyplex (miR-155 CP) <em>in vitro</em>.</div></div><div><h3>Methods</h3><div>The antileishmanial activity of miR-155 CP synthesized by the coacervation method was assessed against <em>Leishmania major</em> by analyzing the IL-12 and IL-10 secretion, nitric oxide (NO) and urea production, as well as cell death rate on infected RAW 264.7 cells <em>in vitro.</em></div></div><div><h3>Results</h3><div>The polyplexes were produced with high transfection efficiency. MiR-155 was up-regulated over 20-fold in healthy macrophages and 6-fold among infected macrophages. The mean cell death index among treated infected cells (65.5 ± 4.5) increased significantly compared to the infected control group (25±5). Treatment with miR-155 CP triggered the production of IL-12 and NO among uninfected and infected macrophages.</div></div><div><h3>Conclusion</h3><div>Collectively, our findings suggest that induction of miR-155 expression via miR-155 CP may influence cellular mechanisms in two ways: eliminating infected macrophages and enabling uninfected ones to manage the parasite.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107674"},"PeriodicalIF":2.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological evidence of epizootic hemorrhagic disease and serotypes of epizootic hemorrhagic disease virus in Pakistan","authors":"Muhammad Ishaq ,&nbsp;Syed M. Jamal ,&nbsp;Liana Teodori ,&nbsp;Alessandra Leone ,&nbsp;Barbara Bonfini ,&nbsp;Massimo Spedicato ,&nbsp;Giovanni Savini","doi":"10.1016/j.actatropica.2025.107675","DOIUrl":"10.1016/j.actatropica.2025.107675","url":null,"abstract":"<div><div>This study investigated seroprevalence and serotypes of Epizootic hemorrhagic disease virus (EHDV) in ruminants in North-Western Pakistan. A total of 616 serum samples were tested for the presence of antibodies against EHDV and neutralizing antibodies against 7 EHDV serotypes (EHDV-1, 2, 4, 5, 6, 7 and 8) using competitive ELISA and VNT, respectively. The overall seroprevalence of EHDV was found to be 35.9 %. Seroprevalence of EHDV in cattle, buffalo and goats was found as 66.9 %, 21.3 % and 4.1 %, respectively. In multivariate analysis, the seroprevalence of EHDV was found 12.8 (95 % CI = 6.1–27) times higher in cattle than buffalo and 3.3 (95 % CI = 1.4–10) times higher in buffalo than goats. The seroprevalence was found to be 6 (95 % CI = 3.2–11.3) times higher in old animals than in young ones. The seroprevalence was recorded as 4.8 (95 % CI = 2.3–10) and 2.1 (95 % CI = 1.3–3.6) times higher in the Southern and Central zones, respectively than in the Northern zone of the study area. Neutralizing antibodies against six different serotypes of EHDV (EHDV-1, 2, 5, 6, 7 and 8) were detected. Neutralizing antibodies against EHDV-6 was found to be the most prevalent (62.1 %) and those against EHDV-1 as the least prevalent (11.2 %). This study shows that Epizootic hemorrhagic disease (EHD) is prevalent in Pakistan and at least six different serotypes of EHDV have circulated or are circulating in the country. Virological studies are required to investigate the currently circulating serotypes of EHDV in the country.</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107675"},"PeriodicalIF":2.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “Comparative analysis of population genetics between Schistosoma japonicum-infected and uninfected Oncomelania hupensis snails using microsatellites”","authors":"Meng-Rui Huang ,&nbsp;Yu-Xin Qi ,&nbsp;Qing Xu,&nbsp;Ze-Ting Liu,&nbsp;Xiao-Yan Wu,&nbsp;Han-Xiang Zhang,&nbsp;Da-Bing Lu","doi":"10.1016/j.actatropica.2025.107658","DOIUrl":"10.1016/j.actatropica.2025.107658","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107658"},"PeriodicalIF":2.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neglecting animal sporotrichosis notification in Brazil: A challenge for one health surveillance","authors":"Paulo Ricardo Martins-Filho ,&nbsp;Silvia Maria da Silva Sant'Ana Rodrigues ,&nbsp;Thialla Andrade Carvalho ,&nbsp;Francy Waltilia Cruz Araújo ,&nbsp;André Luiz de Jesus Morais ,&nbsp;Ana Fátima Souza Melo de Andrade ,&nbsp;Danilo Santos de Sousa ,&nbsp;Martha Débora Lira Tenório ,&nbsp;Janiele de Sá Ferreira ,&nbsp;Roseane Nunes de Santana Campos","doi":"10.1016/j.actatropica.2025.107665","DOIUrl":"10.1016/j.actatropica.2025.107665","url":null,"abstract":"","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"267 ","pages":"Article 107665"},"PeriodicalIF":2.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}