Biomedical materials (Bristol, England)最新文献

{"title":"Evaluation and processing of physical properties of anti-calcified glutaraldehyde-treated three-dimensional cultured cartilage tissues.","authors":"Yohei Kitaguchi, Tomoyuki Ota, Tomoka Takao, Ryosuke Iwai, Takeshi Moriwaki, Yuki Fujisawa, Daisuke Yamada, Takeshi Takarada","doi":"10.1088/1748-605X/addbb5","DOIUrl":"10.1088/1748-605X/addbb5","url":null,"abstract":"<p><p>Reconstruction using cartilage tissue is necessary to address deformities of the nose, ears, and maxillofacial region in several cases. However, autologous cartilage tissue transplantation is limited in the amount that can be harvested owing to invasiveness to the human body. Moreover, artificial materials such as implants cannot be used in many situations, given their potential to induce reactions to foreign bodies. Therefore, there is a growing demand for biomaterials that are less likely to cause foreign body reactions. Given that a tissue with a functionally superior three-dimensional (3D) structure can replace autologous tissue and artificial materials, we have developed a 3D cultured cartilage tissue without scaffolding material and are working toward its practical application. To achieve an off-the-shelf product that allows prolonged storage, the tissue was fixed with glutaraldehyde to maintain high strength for subsequent processing and management. Although tissue fixation with glutaraldehyde may cause calcification due to the deposition of calcium phosphate, calcification can be prevented by washing with high-concentration ethanol. We generated 3D cultured cartilage tissues using induced pluripotent stem cell-derived limb bud mesenchymal cells and an original cell self-culture aggregation method. The generated tissues were subjected to an anti-calcification treatment with glutaraldehyde and 80% ethanol. The treated tissue had improved stability and strength with minimal calcification. The tissue retained its physical properties that were effectively processable and could be processed into an ear-like shape.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of copper doped<i>β</i>-TCP and GB14 coatings produced via high velocity suspension flame spraying-a biocompatibility and antimicrobial activity study.","authors":"Long-Quan R V Le, Maria Carolina Lanzino, Anika Höppel, Hermann O Mayr, Ali Al-Ahmad, Bettina Spitzmüller, Moritz Mayr, Anna Baghnavi, Michael Seidenstuecker","doi":"10.1088/1748-605X/adda82","DOIUrl":"10.1088/1748-605X/adda82","url":null,"abstract":"<p><p>Implant-associated infections and aseptic loosening of prosthesis due to insufficient secondary stability continue to present a challenging issue in arthroplasty. Potential solutions include bioactive coatings to promote osseointegration. With this in mind, this study aims to investigate and compare thin bioactive and bioresorbable<i>β</i>-tricalcium phosphate (<i>β</i>-TCP) and calcium alka-li orthophosphate (GB14) coatings, both produced via high velocity suspension flame spraying. To achieve an additional antibacterial effect and to prevent infections through aerosolized contamination, Cu-doped<i>β</i>-TCP supraparticles (SP) are incorporated into the coatings.<i>β</i>-TCP and GB14 coatings with 0.5 wt.% Cu-doped<i>β</i>-TCP SP each were investigated. According to ISO EN 10993-14, a degradation test was performed in TRIS-buffer at pH 7.4 over 120 h. Biocompatibility testing was performed on human osteoblasts using live/dead staining on days 1, 3 and 7 to simultaneously visualize viable and non-viable cells, while cytotoxicity was assessed over a 3 d period with the cytotoxicity assay. To evaluate the antibacterial efficacy, safe airborne antibacterial assays using<i>S. aureus</i>and<i>E. coli</i>were performed. Our investigations demonstrate that Cu is released from the coatings over a period of 120 h. The released Cu-amount results in a significant reduction in colony forming units across all coatings, while only negligibly imparing the behavior of the human osteoblasts. Both coatings exhibit high biocompatibility, with cell counts varying depending on the amount of Cu released. Cytotoxicity testing showed no cytotoxic effects for the samples examined.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration responses of Ankaferd Blood Stopper, platelet-rich-plasma, and<i>Momordica charantia</i>on sciatic nerve injury in the obese rats.","authors":"Mehmet Emin Önger, Stefano Geuna, Abubaker El Elhaj, Stefania Raimondo, Suleyman Kaplan","doi":"10.1088/1748-605X/add97c","DOIUrl":"10.1088/1748-605X/add97c","url":null,"abstract":"<p><p>Obesity is a prevalent and potentially fatal disorder in industrialized nations, often due to an imbalance between calorie intake and energy expenditure. The study investigates the effects of Ankaferd Blood Stopper (ABS), platelet-rich plasma (PRP), and<i>Momordica charantia</i>(MC) on obese rats with sciatic nerve injuries using stereological and electron microscopic techniques. Twenty-four female Sprague Dawley rats, aged 8-10 weeks, were divided into three groups: the obese gap ABS group (OGABS), the obese gap PRP group (OGPRP), and the obese gap MC group (OGMC). A five-mm nerve block was resected approximately 10 mm above the nerve branch. The gap region was then surrounded and closed by a collagen membrane in tube form. Materials such as ABS, PRP, and MC were injected into the tubes of each group. Electromyography and histological procedures were performed after 12 weeks of surgery to investigate their structural repair and functional promotion of nerve regeneration. A significant increase in the number of myelinated axons in OGMC was found. In addition, the latency values in OGMC were more critical than in the OGABS and OGPRP groups, indicating better myelination. PRP and ABS positively affected nerve gap repair, favoring regenerating unmyelinated axons. Due to increased amplitude values, PRP and ABS have also improved axonal area regeneration. Collagen tubes containing ABS, PRP, and MC may help repair and close peripheral nerve gaps in obese rats, which could be beneficial in closing peripheral nerve resection and demyelination. The study's findings indicate that these collagen tubes can have a therapeutic effect on peripheral nerve regeneration in obese models.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel innovations for 3D organoid culture.","authors":"Yicheng Feng, Dongyang He, Xiao An","doi":"10.1088/1748-605X/add82d","DOIUrl":"10.1088/1748-605X/add82d","url":null,"abstract":"<p><p>Organoids are functional cell-tissue complexes that mimic structural and functional characteristics of organs<i>in vitro</i>in three dimensions (3D). Mimicking the natural extracellular matrix (ECM) environment is critical for guiding stem cell fate within organoid cultures. Current organoid cultures predominantly utilize animal- or tumor-derived ECMs such as dECMs and Matrigel. However, these materials introduce batch variability and uncertainty in composition, which hinders reproducibility. In contrast, naturally derived and synthetic hydrogels with excellent biocompatibility offer precise and adjustable compositions, along with tunable mechanical properties, thereby providing robust support for organoid development and maturation. We explore innovative hydrogel designs tailored specifically for organoid cultures, emphasizing the influence and meticulous control of functional hydrogels on organoid formation, differentiation, and maturation processes. Furthermore, the review highlights the potential of functionalized hydrogel scaffolds to advance both research and industrial applications in tissue and organ engineering. As research progresses, investigations will further concentrate on improving the adjustable properties, expanding their scope of application, and more biologically compatible gelation strategies of hydrogels.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapidly curable zinc chondroitin sulfate @ methacrylated hyaluronic acid hydrogel: a novel photocurable biomaterial tailored for emergency wound management.","authors":"Shiman Li, Qili Sun, Huicheng Cao, Chunan Lu, Jialin Yu, Kangyu Chen, Chuqing Tang, Zimo Li, Shuaishuai Cao, Tenghui Zeng, Bin Tang","doi":"10.1088/1748-605X/add63b","DOIUrl":"10.1088/1748-605X/add63b","url":null,"abstract":"<p><p>In today's emergency medical field, rapid hemostasis and wound healing technologies are of paramount importance. However, traditional methods, although effective, have limitations such as slow hemostasis, susceptibility to infection, and unsuitability for irregular wounds. To address these issues, this study combined methacrylated hyaluronic acid (HAMA) with zinc chondroitin sulfate (CSZn) to successfully develop a novel sprayable photocurable hydrogel, CSZn@HAMA. Material characterization confirmed that CSZn was effectively loaded into HAMA, while retaining HAMA's photocurable and sprayable properties. This allows the CSZn@HAMA hydrogel to rapidly solidify and form a tight protective film over the wound after spraying. Further cell experiments demonstrated that this hydrogel has significant anti-inflammatory effects and can effectively promote collagen production and angiogenesis. Therefore, CSZn@HAMA has emerged as a promising biomaterial for wound management in emergency medical care.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dressing antibacterial platinum loaded polyurethane materials by a stable hydrophilic coating with robust antithrombotic properties.","authors":"Shuai Gao, Zheng Liu, Wei Zeng, Xi Liu, Fanjun Zhang, Dimeng Wu, Yunbing Wang","doi":"10.1088/1748-605X/add6fa","DOIUrl":"10.1088/1748-605X/add6fa","url":null,"abstract":"<p><p>Thrombus formation and infection resulting from blood contact with medical materials represent significant clinical complications characterized by a mutually reinforcing relationship between the two phenomena. Consequently, the development of hydrophilic coatings that simultaneously release bactericidal agents and exhibit passive antithrombotic properties is of paramount importance. In this work, we employed a straightforward, easily executable, and amenable to scale-up strategy to synthesize an antibacterial polyurethane matrix containing platinum complex, followed by the construction of a phosphorylcholine-based hydrophilic coating on its surface via surface-initiated polymerization. The antibacterial efficacy arises from the sustained release mechanism of platinum complex ions, while the phosphorylcholine coating exhibits remarkable antithrombotic characteristics. These two functionalities operate both independently and synergistically within the material to provide robust antibacterial and antithrombotic performance.<i>In vitro</i>and<i>in vivo</i>experiments validated the effectiveness of this composite material in inhibiting bacterial growth and preventing thrombus formation. Furthermore, histological analysis along with immunological detection confirmed that the coating material has excellent biocompatibility coupled with anti-inflammatory effects. Therefore, the developed innovative coating with efficient antibacterial and antithrombotic effects shows great promise as an effective strategy for mitigating risks associated with infections and thrombus formation in clinical settings involving blood-contacting medical devices and related implants.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of novel carboxymethyl chitosan-based bioinks for 3D bioprinting of neural tissues.","authors":"Amanda C Juraski, Victor A da Silva, Ruchi Sharma, Adriano R Azzoni, Stephanie M Willerth","doi":"10.1088/1748-605X/add6f9","DOIUrl":"10.1088/1748-605X/add6f9","url":null,"abstract":"<p><p>The formulation of bioinks is critical for successful 3D bioprinting. It influences printability, stability, and cell behavior. One of the main demands in 3D bioprinting is the development of bioink formulations that can balance long-term cell viability and compositional similarities to the extracellular matrix (ECM) with rheological properties for 3D printing. To address this challenge, this study tested new bioinks using carboxymethyl chitosan (N,O-CMCS or O-CMCS), alginate, and fibrin, which are promising biomaterials due to their biocompatibility and likeness to the ECM. 3D bioprinting of neural tissues comes with additional challenges because neural cells are highly sensitive to environmental conditions. Therefore, we optimized our bioink formulations for the 3D bioprinting of neural progenitor cells derived from human induced pluripotent stem cells (hiPSC-NPC). Here we report a neural tissue constructed 3D bioprinted with a hiPSC-NPC-laden 1% N,O-CMCS, 1% alginate, and 20 mg ml^-1fibrin. This formulation exhibited uniform consistency and minimal extrusion force fluctuations (approximately 8 KPa), indicating homogeneity and optimal printability using an extrusion-based bioprinter. In contrast, O-CMCS formulations did not support neural tissue differentiation while higher concentrations of N,O-CMCS or alginate (3% w/v) resulted in increased viscosity and poorly defined scaffolds. The optimized bioink demonstrated significant water retention, swelling up to 15 times its original weight without losing structural integrity, thus providing a conducive environment for cell culture. Live/dead staining revealed over 60% cell viability over 30 d, underscoring its suitability for long-term cell applications. Immunocytochemistry confirmed that the optimized N,O-CMCS-based bioink effectively guided cells toward further differentiation into neurons and astrocytes, thus forming a 3D bioprinted construct that is able to replicate different neural cell types found in the neural tissue. The optimized bioink described in this study lays the groundwork for future works that will focus on detailing how different CMCS groups affect tissue maturation and functionality in 3D bioprinted constructs that can potentially be used for future neural tissue modeling and drug screening.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red blood cell membrane-camouflaged nanocarriers for the delivery of piperlongumine to treat triple-negative breast cancer.","authors":"Chenxi Li, Jiaxin Zhang, Xianxian Yao, Yuxin Huang, Yichen Zhang, Wuli Yang","doi":"10.1088/1748-605X/add4da","DOIUrl":"10.1088/1748-605X/add4da","url":null,"abstract":"<p><p>The application of the conventional drugs for triple-negative breast cancer (TNBC) treatment in chemotherapy is limited due to their intrinsic drawbacks such as short drug half-life, lack of tumor selectivity and systemic toxicity. Herein, an effective nanoparticle drug delivery system (NDDS) of red blood cell (RBC) membrane-camouflaged piperlongumine (PL)-loaded iron oxide (Fe₃O₄) magnetic nanoparticles (Fe₃O₄-PL@RBC) was rationally designed as an effective drug delivery platform for<i>in vivo</i>TNBC treatment. The Fe₃O₄-PL@RBC showed considerable cytotoxicity against MDA-MB-231 cells, inducing intracellular accumulation of reactive oxygen species, mitochondrial dysfunction and apoptosis. Furthermore, transcriptomic analyses and western blotting analysis demonstrated that the Fe₃O₄-PL@RBC induced apoptosis through the inhibition of PI3K/AKT/mTOR pathway and downregulation of Bcl-2 protein. In MDA-MB-231 tumor models, the RBC membrane coating in Fe₃O₄-PL@RBC effectively prolonged the circulation time and sufficient enrichment at the tumor sites. And the Fe₃O₄-PL@RBC significantly inhibited tumor growth with good biosafety. This study provides guidance for the rational design of effective Fe₃O₄-based NDDS for TNBC treatment.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in anti-tumor mechanisms and biological applications of vanadium compounds.","authors":"Xinhao Dang, Yan Xue, Siying Zhang, Menglan Chen, Kangliang Sheng, Jie Ma, Shan Gao, Yongzhong Wang","doi":"10.1088/1748-605X/add3e5","DOIUrl":"https://doi.org/10.1088/1748-605X/add3e5","url":null,"abstract":"<p><p>Vanadium, a transition metal, has emerged as a promising element in the development of therapeutic drugs. While not an essential element for life, vanadium compounds have demonstrated significant potential as anticancer agents. Current evidence suggests that these compounds exert their anti-tumor effects through multiple mechanisms, including DNA damage, cell cycle regulation, induction of apoptosis and autophagy, inhibition of metastasis and invasion, and disruption of mitochondrial function. Furthermore, vanadium compounds have shown efficacy against a wide range of cancers, such as melanoma, breast, colorectal, pancreatic, liver, and central nervous system tumors, as well as oral squamous cell carcinoma. This review aims to comprehensively examine the anti-tumor properties and underlying mechanisms of various vanadium compounds while also providing an overview of their current biological applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprosthetic heart valves with zwitterionic copolymer grafting to improve the properties of endothelialization and anti-calcification.","authors":"Daoyang Zhu, Yu Luo, Shenyu Huang, Lie Ma","doi":"10.1088/1748-605X/add3e7","DOIUrl":"https://doi.org/10.1088/1748-605X/add3e7","url":null,"abstract":"<p><p>Heart valve replacement surgery has been the most effective treatment for severe valvular heart disease. Bioprosthetic heart valves (BHVs) crosslinked by glutaraldehyde (GA) have non-negligible advantages in clinical applications. However, structural valve degeneration, calcification, insufficient re-endothelialization and other factors lead to a shortened service life of BHVs. In this study, GA-crosslinked decellularized heart valves (GADHVs) were grafted with zwitterionic copolymer (PSBG) of [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide and glycidyl methacrylate, and further treated with Arg-Glu-Asp-Val (REDV) peptide to obtain REDV-PSBG-GADHVs with anti-fouling ability and endothelial cell affinity. REDV-PSBG-GADHVs exhibited good collagen stability, reliable mechanical property and excellent hemocompatibility. Moreover,<i>in vitro</i>and<i>in vivo</i>experiments demonstrated that REDV-PSBG-GADHVs exhibited better endothelialization property, lower immune responses and reduced calcification than GADHVs. This modified strategy for heart valve fabrication, which can improve the effect of anti-calcification and endothelialization while maintaining the original advantages of BHVs, shows great potential for application in heart valve replacement.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}