Biomedical materials (Bristol, England)最新文献

{"title":"3D printed skin dressings manufactured with spongin-like collagen from marine sponges: physicochemical properties and<i>in vitro</i>biological analysis.","authors":"Amanda de Souza, Gustavo Oliva Amaral, Giovanna do Espirito Santo, Karolyne Dos Santos Jorge Sousa, Cintia Cristina Santi Martignago, Lais Caroline Souza E Silva, Lindiane Eloisa de Lima, Daniel Vitor de Souza, Matheus Almeida Cruz, Daniel Araki Ribeiro, Renata Neves Granito, Ana Claudia Muniz Renno","doi":"10.1088/1748-605X/adad29","DOIUrl":"10.1088/1748-605X/adad29","url":null,"abstract":"<p><p>The search for innovative materials for manufacturing skin dressings is constant and high demand. In this context, the present study investigated the effects of a 3D printed skin dressing made of spongin-like collagen (SC) extract from marine sponge (<i>Chondrilla caribensis</i>), used in 3 concentrations of SC and alginate (C1, C2, C3). For this proposal, the physicochemical, morphological and<i>in vitro</i>biological results were investigated. The results demonstrated that, after immersion, C2 presented a higher mass loss and C3 present a higher pH in experimental periods. Also, a higher porosity was observed for C1 and C2 skin dressings, with a higher swelling ratio for C2. For Fourier transform infrared, peaks of Amide A, -CH2, -COOH and C-O-C were seen. Moreover, the macroscopic image demonstrated a skin dressing with rough surface and grayish color that is naturally observed in<i>Chondrilla caribensis</i>. For scanning electron microscopy analysis the presence of pores could be observed for all skin dressings, with fibers disposed in layers. The<i>in vitro</i>analyses demonstrated the viability of HFF-1 and L929 cell lines 70% of the values found for cell proliferation compared to Control Group. Furthermore, the cell adhesion analysis demonstrated that both cell lines adhered to the 3 different skin dressings and non-cytotoxicity was observed. Taking together, all the results suggest that the skin dressings are biocompatible and present non-cytotoxicity in the<i>in vitro</i>studies, being considered a suitable material for tissue engineering proposals.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Material and technique fundamentals of nano-hydroxyapatite coatings towards biofunctionalization: a review.","authors":"Durgesh Phogat, Shikha Awasthi","doi":"10.1088/1748-605X/adac97","DOIUrl":"10.1088/1748-605X/adac97","url":null,"abstract":"<p><p>Hydroxyapatite (HAp) nanocoatings on titanium alloys (e.g. Ti6Al4V) have been used for prosthetic orthopaedic implants in recent decades because of their osseointegration, bioactivity, and biocompatibility. HAp is brittle with low mechanical strength and poor adhesion to metallic surfaces, which limits its durability and bioactivity. Surface modification techniques have alleviated the imperfections in biomaterials by coating the substrate. Several methods for improving the characteristics of implants, such as physical vapour deposition, the thermal spray method, the sol-gel method, microarc oxidation, and electrochemical deposition methods, have been discussed in this review. These processes provide mechanical strength without sacrificing biocompatibility and may lead to the development of new ideas for future research. This review discusses various selective additives, including carbon allotropes, ceramic materials, metallic materials, and multiple materials, to enhance tribological characteristics, biocompatibility, wear resistance, and mechanical strength. This review focuses on the fabrication of nano-HAps as coatings using selective deposition methods with controlled deposition parameters, paying special attention to recent developments in bone tissue engineering. This report is organized in such a way that it may inspire further research on surface modifications during medical treatment. The present review may help prospective investigators understand the importance of surface modifications for obtaining excellent implantation performance.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A BMP-2 sustained-release scaffold accelerated bone regeneration in rats via the BMP-2 consistent activation maintained by a non-sulfate polysaccharide.","authors":"Jinghe Sun, Rongchun Gao, Ningbo Qin, Jingfeng Yang","doi":"10.1088/1748-605X/adad28","DOIUrl":"https://doi.org/10.1088/1748-605X/adad28","url":null,"abstract":"<p><p>Bone morphogenetic protein 2 (BMP-2) and a polysaccharide (SUP) were embedded in the calcium phosphate cement (CPC) scaffold, and the bone repair ability was evaluated. The new scaffolds were characterized using x-ray diffraction, Fourier transform-infrared, scanning electron microscopy, and energy dispersive spectroscopy analyses. CPC-BMP2-SUPH scaffold promoted the BMP-2 release by 1.21 folds of the CPC-BMP2 scaffold on day 3. SUP sustained the release of BMP-2 within 21 d. It enhanced alkaline phosphatase activity by 25.9% in comparison to the CPC scaffold. These results suggest that the SUP consistently activated and sustained BMP-2 release<i>in vitro</i>. Furthermore, the CPC-BMP2-SUPH scaffold activated the BMP-2/Smads and runt-related transcription factor 2 (Runx-2) pathways in MC3T3-E1 cells to up-regulate the levels of osteogenic relative genes (BMP-2, bone sialoprotein, collagen 1, osteocalcin, osteopontin, and Runx-2). The<i>in vivo</i>result showed that the bone defect area in the CPC-BMP2-SUPH scaffold-treated Sprague-Dawley rats lessened significantly compared with the CPC group after 4 weeks. CPC-BNP2-SUPH scaffold also improved collagen regeneration in bone. The bone surface and bone volume in the CPC-BMP2-SUPH group improved by 3.68 and 2.17-fold compared with the CPC group, respectively. In conclusion, the CPC-BMP2-SUPH scaffold represents a novel biomaterial capable of accelerating osteoblast differentiation and promoting bone injury repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold nanocages co-assembled with<i>Spinacia oleracea</i>extract combined photothermal/photodynamic therapy in 4T1 breast cancer cell line.","authors":"Dheeraj Dehariya, Anindita Tarafdar, Monika Pebam, Sri Amruthaa Sankaranarayanan, Sajmina Khatun, Aravind Kumar Rengan","doi":"10.1088/1748-605X/ada83e","DOIUrl":"10.1088/1748-605X/ada83e","url":null,"abstract":"<p><p>Photothermal therapy (PTT) and photodynamic therapy (PDT) have been emerging as potential alternatives to conventional cancer treatment modalities. Gold nanoparticles, owing to their surface plasmon resonance properties, have been promising in cancer phototherapies, and extracts from potent medicinal plants are commonly employed for the green synthesis of various nanoparticles. Some researchers have used photosensitizers like chlorophyll to promote reactive oxygen species generation. In this research, the photothermal ability of gold and the photon-absorbing capability of chlorophyll derived from<i>Spinacia oleracea</i>(<i>S. oleracea</i>) are combined to achieve the optimum results. Herein, we have synthesized the gold nanocages(AuNCs) co-assembled with<i>S. oleracea</i>extract (SPAuNCs; 70 ± 10 nm) to be employed as a PTT and PDT agent to treat triple-negative breast cancer. This study found that SPAuNCs are promising PTT and PDT agents against breast cancer cell line.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atomic-layer-deposition application for antibacterial coating of biomedical materials: surgical sutures.","authors":"Ilmutdin M Abdulagatov, Visampasha Yu Khanaliev, Razin M Ragimov, Abai M Maksumova, Мagomed А Khamidov, Naida M Abdullaeva, Naida R Mollaeva","doi":"10.1088/1748-605X/ada841","DOIUrl":"10.1088/1748-605X/ada841","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Suture-associated surgical site infection (SSI) causes bacterial pathogens to colonize on the suture surface that are highly resistant to antibiotic treatment. Conventional suture materials used in surgical practice are causing complications such as infection and chronic inflammation. Surgical suture materials with antibacterial coatings are widely used in surgical practice. However, all the widely used antibacterial agents are not permanent (limited lasting) due to their instability and release depending on environmental conditions (pH or temperature, for example). Therefore, more long-lasting (low-dose) and effective antibacterial function materials are required. In the present work, we proposed a new material and method of antibacterial coating the surgical sutures based on the atomic layer deposition (ALD) technique to enhance its antibacterial activity for treatment of the SSI. We have proposed applying a vanadium-doped TiO&lt;sub&gt;2&lt;/sub&gt;nanofilm (hybrid nanomaterial, TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;) with 27.5 nm thickness to enhance the antibacterial property of surgical sutures using the ALD technique. We have illustrated that a base coating of Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt;(seed layer) applied to the suture surface, which directly contacts the polypropylene (PP) suture, improves the adhesion of the deposited antibacterial material TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;. This provides a long-lasting antibacterial effect on the suture (a prolonged antibacterial effect of the coating material), i.e. increases the stability of the deposition (stable in water, air, in the human body, in different pH mediums, and at temperatures up to 70 °C). The sutures did not deteriorate after several wash cycles with sterilizing solvents. Also, the antibacterial agent (TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;) is nontoxic. The concentration of vanadium in the film is below the toxicity limits due to the low diffusivity of vanadium and high adhesion with the base coating material (Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt;). Sutures coated with V-doped TiO&lt;sub&gt;2&lt;/sub&gt;were characterized using scanning electron microscopy images, and elemental analysis was performed using energy dispersive spectroscopy Spectroscopy. The antibacterial activity of TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;coated sutures against two types of microorganisms,&lt;i&gt;E. coli&lt;/i&gt;and Proteus vulgaris (&lt;i&gt;Pr. Vulgaris&lt;/i&gt;) was compared to that of noncoated sutures. The quantitative assessment of antibacterial activity of suture materials with and without ALD nanocoating TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;against&lt;i&gt;E. coli&lt;/i&gt;and&lt;i&gt;Pr. Vulgaris&lt;/i&gt;has been performed. No growth of bacteria around the suture material with antibacterial TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;ALD nanocoating throughout the entire observation period of 48 and 72 h was observed. However, after 48 h, the concentration of bacteria of the&lt;i&gt;E. Coli&lt;/i&gt;around the suture material without ALD TiVO&lt;i&gt;x&lt;/i&gt;nanocoating on nutrient agar was 5.5 ± 0.3 Log CFU cm&lt;sup&gt;-3&lt;/sup&gt;, and after 72 h it was 8.0 ± 0.5 Log CFU cm&lt;sup&gt;-3","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ficus carica leaves extract loaded PLGA nanoparticles: preparation, characterization, and in vitro anticancer activity on TFK-1 cell line.","authors":"Bushra Aziz, Esmeralda Dc Bosman, Kim Mg van der Wurff-Jacobs, Cornelus F van Nostrum, Ahmat Khurshid","doi":"10.1088/1748-605X/adaff7","DOIUrl":"https://doi.org/10.1088/1748-605X/adaff7","url":null,"abstract":"<p><p>Ficus Carica extract (FC) is a natural herb that has received a lot of interest in cancer treatment due to its potential anticancer activities against various malignancies. However, due to FC's low bioavailability and low solubility, its clinical use as an anti-cancer medicine is constrained. The current study aimed to prepare FC-loaded PLGA nanoparticles (NPs) for cancer treatment. Prepared NPs were characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), zeta potential, and transmission electron microscopy (TEM). The results showed that the spherical FC-loaded PLGA NPs had a particle size (162 ± 0.7 nm), a polydispersity index (0.08 ± 0.005), and zeta potential (-4.7 ± 0.6 mV). The encapsulation and loading efficiency was found to be 56 ± 2.3% and 14 ± 1.5 %, respectively. A drug release study indicated a diffusion-based release profile. Cytotoxicity was evaluated on TFK-1 cell line, which showed that both free FC and corresponding FC concentrations in NPs were cytotoxic. Cell cycle analysis showed that the FC arrests the cells in G0/G1 phase, and the cell arrest rate is higher in FC-loaded NPs as compared to free form. A phototoxicity study also showed that the phototoxicity of FC-loaded PLGA NPs was time-dependent and enhanced in comparison to free FC. The study's results demonstrated that FC-encapsulated PLGA NPs are promising for cancer therapy as a phyto- and phototherapeutic agent-based system.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and development of vaginal wall mimicking poly(<i>ϵ</i>-caprolactone) based nanofibrous prosthetic mesh for pelvic organ prolapse: evaluation of biocompatibility and antibacterial ability.","authors":"Preethi Arul Murugan, Jayesh Bellare","doi":"10.1088/1748-605X/ada2d0","DOIUrl":"10.1088/1748-605X/ada2d0","url":null,"abstract":"<p><p>Mechanical non-conformance of conventionally used transvaginal non-degradable meshes has led to complications such as organ perforation, dyspareunia caused by mesh stiffness and stress shielding. In this study, we have solved the dire need to mimic the mechanical properties of the vaginal wall by designing and developing a soft and elastic mesh made of polycaprolactone (PCL), citric acid modified polyethylene glycol (PEGC) and zinc oxide (ZnO) prepared through electrospinning and tested<i>in vitro</i>and<i>in vivo</i>. The mesh containing 90:10:0.1 of PCL, PEGC and ZnO (PEGC-15 0.1ZnO mesh) conforms to the mechanical properties of the vaginal wall of the pelvic floor, has a burst strength of ∼35 N even after gamma-sterilization and 28 d of degradation in<i>in vitro</i>.<i>In vitro</i>studies using adipose-derived stem cells revealed that the PCL-PEGC-15 0.1ZnO meshes were biocompatible and supported higher collagen production than commercial mesh.<i>An in vitro</i>bacterial adhesion study showed a 2-log reduction compared to commercially available mesh for prolapse treatment. Initial biocompatibility assessment in a rabbit model also showed that the PCL-PEGC-15 0.1ZnO mesh is biocompatible and supports fibrosis throughout the mesh. The softness and flexibility of the PCL-PEGC-15 0.1ZnO mesh based on<i>in vitro</i>trials and initial<i>in vivo</i>trials show that the mesh has a potential clinical impact for pelvic floor repair treatment.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i>evaluation of bioabsorbable poly(lactic acid) (PLA) and poly-4-hydroxybutyrate (P4HB) warp-knitted spacer fabric scaffolds for osteogenic differentiation.","authors":"Flavia Caronna, Skander Limem, Ly Dang Khoa Do, William Ronan, Eimear B Dolan","doi":"10.1088/1748-605X/ada85d","DOIUrl":"10.1088/1748-605X/ada85d","url":null,"abstract":"<p><p>Bioabsorbable textile scaffolds are promising for bone tissue engineering applications. Their tuneable, porous, fibre-based architecture resembles that of native extracellular matrix, and they can sustain tissue growth while being gradually absorbed in the body. In this work, immortalized mouse calvaria preosteoblast MC3T3-E1 cells were cultured<i>in vitro</i>on two warp-knitted bioabsorbable spacer fabric scaffolds made of poly(lactic acid) (PLA) and poly-4-hydroxybutyrate (P4HB), to investigate their osteogenic properties. Scaffold structure and yarn properties were characterized after manufacturing. Cells were seeded on the two scaffolds and treated with osteogenic media for up to 35 days. Both scaffolds supported similar cell growth patterns, featuring a higher cell density on multifilament yarns, which could be beneficial to drive cell proliferation or related phenomena in localized area of the construct. The increase in alkaline phosphatase activity and the calcium deposition observed on some PLA and P4HB scaffolds after 28 and 35 days of culture, confirm their potential to support MC3T3-E1 cells differentiation, however inconsistent mineralization was observed on the scaffolds. Due to their structural and morphological features, ability to support cell attachment and growth, and their limited osteogenic potential, these PLA and P4HB bioabsorbable textile scaffolds are recommended for further investigation for bone tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing osteogenic properties with gelatin/chitosan hydrogel encapsulating lithium-coated titanium oxide hollow sphere particles loaded with quercetin.","authors":"Qingjie Wang, Liang Zhang","doi":"10.1088/1748-605X/adae6e","DOIUrl":"https://doi.org/10.1088/1748-605X/adae6e","url":null,"abstract":"<p><p>Metallic oxides especially lithium and titanium oxides are well known for their osteogenic properties. When combined in the right proportions, metallic oxides can have an even greater impact. However, releasing ions from oxides can lead to oxidative stress, which is harmful to cell growth. By reducing oxidative stress, we can enhance these ions' therapeutic and bone-forming properties. In our study, we have developed a novel combination of titanium oxide coated with lithium oxide to release ions simultaneously. We engineered hollow sphere titanium oxide particles to carry Quercetin (QC), a natural antioxidant. These particles were then incorporated into a gelatin/chitosan-based hydrogel, which was further functionalized with carbon nanotubes (CNTs) which induced conductivity and improved mechanical properties. In drug release experiments, we found that QC was released steadily from the hydrogel, in contrast to a control group where the drug was simply mixed in with hydrogel indicating the significance of a secondary carrier. Additionally, our cytotoxicity tests demonstrated the importance of delivering QC alongside lithium and titanium ions, as this combination reduced toxicity and enhanced bone-forming activity. Finally, our study showed that the hydrogel containing drug-loaded hollow sphere particles was able to promote bone formation, as evidenced by osteogenic differentiation studies. This innovative approach holds promise for improving bone regeneration therapies in the future.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atelocollagen-based hydrogel loaded with<i>Cotinus coggygria</i>extract for treatment of type 2 diabetic wounds.","authors":"Candan Yilmaz Ozdogan, Halime Kenar, Huseyin Uzuner, Aynur Karadenizli","doi":"10.1088/1748-605X/ada7b5","DOIUrl":"10.1088/1748-605X/ada7b5","url":null,"abstract":"<p><p>Diabetes, a chronic metabolic disease, causes complications such as chronic wounds, which are difficult to cure. New treatments have been investigated to accelerate wound healing. In this study, a novel wound dressing from fibroblast-laden atelocollagen-based hydrogel with<i>Cotinus coggygria</i>extract was developed for diabetic wound healing. The antimicrobial activity of<i>C. coggygria</i>hexane (H), dichloromethane (DCM), dichloromethane:methanol (DCM-M), methanol (M), distilled water (DW) and traditional (T) extracts against<i>Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis</i>and<i>Candida albicans</i>, as well as their cytotoxic effects on fibroblasts were determined. While fibroblast growth was significantly (<i>p</i>< 0.05) promoted with DCM (121.41 ± 1.04%), M (109.40 ± 5.89%) and DW (121.83 ± 6.37%) extracts at their lowest concentrations, 2000 μg ml^-1DCM and 7.8 μg ml^-1T extracts had both non-cytotoxic and antifungal effects. An atelocollagen-based hydrogel was produced by thermal crosslinking, and its pore size (38.75 ± 7.67 μm), water content (96.63 ± 0.24%) and swelling ratio (27.21 ± 4.08%) were found to be suitable for wound dressings. A significant increase in the deoxyribonucleic acid amount (28.27 ± 1.41%) was observed in the plain hydrogel loaded with fibroblasts after 9 d of incubation, and the hydrogel had an extensively interconnected cellular network. The hydrogels containing DW and T extracts were applied to wounds generated in an<i>in vitro</i>3D type-2-diabetic human skin model. Although the incubation period was not sufficient for closure of the wounds in either of the treatments, the hydrogel with T extract stimulated more fibroblast migration. In the fibroblast-laden version of the hydrogel with T extract, no wound closure was observed but more keratinocytes migrated to the wound region. These positive outcomes underline the potential of the developed wound dressing as a powerful alternative to improve diabetic wound healing in clinical practice.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}